##gff-version 3
P00383	UniProtKB	Chain	1	78	.	.	.	ID=PRO_0000186435;Note=Dihydrofolate reductase type 2	
P00383	UniProtKB	Binding site	32	36	.	.	.	Ontology_term=ECO:0000269,ECO:0000269,ECO:0007744,ECO:0007744,ECO:0007744;evidence=ECO:0000269|PubMed:17473013,ECO:0000269|PubMed:18052202,ECO:0007744|PDB:2P4T,ECO:0007744|PDB:2RK1,ECO:0007744|PDB:2RK2;Dbxref=PMID:17473013,PMID:18052202	
P00383	UniProtKB	Binding site	66	69	.	.	.	Ontology_term=ECO:0000269,ECO:0000269,ECO:0007744,ECO:0007744,ECO:0007744;evidence=ECO:0000269|PubMed:17473013,ECO:0000269|PubMed:18052202,ECO:0007744|PDB:2P4T,ECO:0007744|PDB:2RK1,ECO:0007744|PDB:2RK2;Dbxref=PMID:17473013,PMID:18052202	
P00383	UniProtKB	Binding site	68	68	.	.	.	Ontology_term=ECO:0000269,ECO:0007744;evidence=ECO:0000269|PubMed:18052202,ECO:0007744|PDB:2RK1;Dbxref=PMID:18052202	
P00383	UniProtKB	Mutagenesis	65	65	.	.	.	Note=No effect. S->A;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:11560482;Dbxref=PMID:11560482	
P00383	UniProtKB	Mutagenesis	67	67	.	.	.	Note=Decreases affinity for NADPH and dihydrofolate about 9-fold. Q->C;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:11560482;Dbxref=PMID:11560482	
P00383	UniProtKB	Mutagenesis	67	67	.	.	.	Note=Increases affinity for dihydrofolate 36-fold. Increases affinity for NADPH 110-fold. Q->H;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:11560482;Dbxref=PMID:11560482	
P00383	UniProtKB	Mutagenesis	68	68	.	.	.	Note=Decreases affinity for dihydrofolate about 5-fold. Decreases affinity for NADPH about 7-fold. I->L%2CM;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:11560482;Dbxref=PMID:11560482	
P00383	UniProtKB	Mutagenesis	69	69	.	.	.	Note=Decreases affinity for dihydrofolate about 9-fold. Decreases affinity for NADPH about 22-fold. Y->F;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:11560482;Dbxref=PMID:11560482	
P00383	UniProtKB	Mutagenesis	69	69	.	.	.	Note=Decreases affinity for dihydrofolate about 9-fold. Decreases affinity for NADPH about 60-fold. Y->H;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:11560482;Dbxref=PMID:11560482	
P00383	UniProtKB	Beta strand	29	36	.	.	.	Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:2RH2	
P00383	UniProtKB	Beta strand	39	46	.	.	.	Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:2RH2	
P00383	UniProtKB	Beta strand	52	62	.	.	.	Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:2RH2	
P00383	UniProtKB	Beta strand	66	70	.	.	.	Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:2RH2	
P00383	UniProtKB	Helix	71	73	.	.	.	Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:2RH2	
P00383	UniProtKB	Beta strand	74	76	.	.	.	Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:2RH2