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P00382 (DYR1_ECOLX) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 85. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Dihydrofolate reductase type 1

EC=1.5.1.3
Alternative name(s):
Dihydrofolate reductase type I
Trimethoprim resistance protein
Gene names
Name:dhfrI
Encoded onPlasmid IncI1 R483 Ref.2 Ref.4
Plasmid pLMO150 Ref.3
Plasmid pLMO229 Ref.3
OrganismEscherichia coli
Taxonomic identifier562 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia

Protein attributes

Sequence length157 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Key enzyme in folate metabolism. Catalyzes an essential reaction for de novo glycine and purine synthesis, and for DNA precursor synthesis By similarity.

Catalytic activity

5,6,7,8-tetrahydrofolate + NADP+ = 7,8-dihydrofolate + NADPH.

Pathway

Cofactor biosynthesis; tetrahydrofolate biosynthesis; 5,6,7,8-tetrahydrofolate from 7,8-dihydrofolate: step 1/1.

Subunit structure

Homodimer.

Sequence similarities

Belongs to the dihydrofolate reductase family.

Contains 1 DHFR (dihydrofolate reductase) domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 157157Dihydrofolate reductase type 1
PRO_0000186420

Regions

Domain2 – 156155DHFR

Natural variations

Natural variant751L → V in plasmid pLMO229.

Sequences

Sequence LengthMass (Da)Tools
P00382 [UniParc].

Last modified July 21, 1986. Version 1.
Checksum: 487F818A40E4991B

FASTA15717,575
        10         20         30         40         50         60 
MKLSLMVAIS KNGVIGNGPD IPWSAKGEQL LFKAITYNQW LLVGRKTFES MGALPNRKYA 

        70         80         90        100        110        120 
VVTRSSFTSD NENVLIFPSI KDALTNLKKI TDHVIVSGGG EIYKSLIDQV DTLHISTIDI 

       130        140        150 
EPEGDVYFPE IPSNFRPVFT QDFASNINYS YQIWQKG 

« Hide

References

[1]"The nucleotide sequence of the trimethoprim-resistant dihydrofolate reductase gene harbored by Tn7."
Fling M.E., Richards C.
Nucleic Acids Res. 11:5147-5158(1983) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Transposon: Tn7.
[2]"Expression of the plasmid-encoded type I dihydrofolate reductase gene in cultured mammalian cells: a novel selectable marker."
Simonsen C.S., Walter M., Levinson A.D.
Nucleic Acids Res. 16:2235-2246(1988) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]"The dhfrI trimethoprim resistance gene of Tn7 can be found at specific sites in other genetic surroundings."
Sundstroem L., Skoeld O.
Antimicrob. Agents Chemother. 34:642-650(1990) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[4]"R plasmid dihydrofolate reductase with a dimeric subunit structure."
Novak P., Stone D., Burchall J.J.
J. Biol. Chem. 258:10956-10959(1983) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 1-34.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X00926 Genomic DNA. Translation: CAA25445.1.
X17477 Genomic DNA. Translation: CAA35509.1.
X17478 Genomic DNA. Translation: CAA35512.1.
PIRRDECD7. S03651.
RefSeqNP_065309.1. NC_002525.1.
YP_004422906.1. NC_015472.1.
YP_190212.1. NC_006671.1.

3D structure databases

ProteinModelPortalP00382.
ModBaseSearch...
MobiDBSearch...

Chemistry

ChEMBLCHEMBL2627.

Proteomic databases

PRIDEP00382.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID10549022.
1238688.
3244885.

Enzyme and pathway databases

UniPathwayUPA00077; UER00158.

Family and domain databases

Gene3D3.40.430.10. 1 hit.
InterProIPR012259. DHFR.
IPR024072. DHFR-like_dom.
IPR017925. DHFR_CS.
IPR001796. DHFR_dom.
[Graphical view]
PfamPF00186. DHFR_1. 1 hit.
[Graphical view]
PRINTSPR00070. DHFR.
SUPFAMSSF53597. SSF53597. 1 hit.
PROSITEPS00075. DHFR_1. 1 hit.
PS51330. DHFR_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameDYR1_ECOLX
AccessionPrimary (citable) accession number: P00382
Secondary accession number(s): P13923
Entry history
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: July 21, 1986
Last modified: July 9, 2014
This is version 85 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways