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Protein

Dihydrofolate reductase type 1

Gene

dhfrI

Organism
Escherichia coli
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Key enzyme in folate metabolism. Catalyzes an essential reaction for de novo glycine and purine synthesis, and for DNA precursor synthesis (By similarity).By similarity

Catalytic activityi

5,6,7,8-tetrahydrofolate + NADP+ = 7,8-dihydrofolate + NADPH.PROSITE-ProRule annotation

Pathwayi: tetrahydrofolate biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes 5,6,7,8-tetrahydrofolate from 7,8-dihydrofolate.
Proteins known to be involved in this subpathway in this organism are:
  1. Dihydrofolate reductase type 8 (dhfrVIII), Dihydrofolate reductase type 9 (dhfrIX), Dihydrofolate reductase (ECs0051), Dihydrofolate reductase (APZ14_00990), Dihydrofolate reductase (dfrB2), Dihydrofolate reductase (dfrA26), Dihydrofolate reductase (dfrA12), Dihydrofolate reductase (dfrA12), Dihydrofolate reductase type A10 (dfrA10), Dihydrofolate reductase (dfrA21), Dihydrofolate reductase (dfr2d), Dihydrofolate reductase (dfr2d), Dihydrofolate reductase type A13 (dfrA13), Dihydrofolate reductase (folA), Dihydrofolate reductase (folA), Dihydrofolate reductase (folA), Dihydrofolate reductase type 2, Dihydrofolate reductase type 2, Dihydrofolate reductase (dfr2a), Dihydrofolate reductase type 2, Dihydrofolate reductase type 7 (dhfrVII), Dihydrofolate reductase (dfrB3), Dihydrofolate reductase (folA), Dihydrofolate reductase (folA), Dihydrofolate reductase type 1 (dhfrI), Dihydrofolate reductase (dfr22), Dihydrofolate reductase type 5 (dhfrV), Dihydrofolate reductase (dfrA12), Dihydrofolate reductase (dfrA12), Dihydrofolate reductase type 15 (dhfrXV), Dihydrofolate reductase (folA), Dihydrofolate reductase (dfrII), Dihydrofolate reductase (dfrA12), Dihydrofolate reductase (dfrA12), Dihydrofolate reductase (dfrA12), Dihydrofolate reductase (dfrA12), Dihydrofolate reductase (folA), Dihydrofolate reductase (dfr22), Dihydrofolate reductase (dfrA12)
This subpathway is part of the pathway tetrahydrofolate biosynthesis, which is itself part of Cofactor biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes 5,6,7,8-tetrahydrofolate from 7,8-dihydrofolate, the pathway tetrahydrofolate biosynthesis and in Cofactor biosynthesis.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Antibiotic resistance, Methotrexate resistance, One-carbon metabolism, Trimethoprim resistance

Keywords - Ligandi

NADP

Enzyme and pathway databases

UniPathwayiUPA00077; UER00158.

Names & Taxonomyi

Protein namesi
Recommended name:
Dihydrofolate reductase type 1 (EC:1.5.1.3)
Alternative name(s):
Dihydrofolate reductase type I
Trimethoprim resistance protein
Gene namesi
Name:dhfrI
Encoded oniPlasmid IncI1 R4832 Publications
Plasmid pLMO1501 Publication
Plasmid pLMO2291 Publication
OrganismiEscherichia coli
Taxonomic identifieri562 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia

Pathology & Biotechi

Chemistry databases

ChEMBLiCHEMBL2627.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001864201 – 157Dihydrofolate reductase type 1Add BLAST157

Interactioni

Subunit structurei

Homodimer.

Chemistry databases

BindingDBiP00382.

Structurei

3D structure databases

ProteinModelPortaliP00382.
SMRiP00382.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini2 – 156DHFRPROSITE-ProRule annotationAdd BLAST155

Sequence similaritiesi

Belongs to the dihydrofolate reductase family.Curated
Contains 1 DHFR (dihydrofolate reductase) domain.PROSITE-ProRule annotation

Phylogenomic databases

KOiK18589.

Family and domain databases

CDDicd00209. DHFR. 1 hit.
Gene3Di3.40.430.10. 1 hit.
InterProiIPR012259. DHFR.
IPR024072. DHFR-like_dom.
IPR017925. DHFR_CS.
IPR001796. DHFR_dom.
[Graphical view]
PfamiPF00186. DHFR_1. 1 hit.
[Graphical view]
PRINTSiPR00070. DHFR.
SUPFAMiSSF53597. SSF53597. 1 hit.
PROSITEiPS00075. DHFR_1. 1 hit.
PS51330. DHFR_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P00382-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKLSLMVAIS KNGVIGNGPD IPWSAKGEQL LFKAITYNQW LLVGRKTFES
60 70 80 90 100
MGALPNRKYA VVTRSSFTSD NENVLIFPSI KDALTNLKKI TDHVIVSGGG
110 120 130 140 150
EIYKSLIDQV DTLHISTIDI EPEGDVYFPE IPSNFRPVFT QDFASNINYS

YQIWQKG
Length:157
Mass (Da):17,575
Last modified:July 21, 1986 - v1
Checksum:i487F818A40E4991B
GO

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural varianti75L → V in plasmid pLMO229. 1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X00926 Genomic DNA. Translation: CAA25445.1.
X17477 Genomic DNA. Translation: CAA35509.1.
X17478 Genomic DNA. Translation: CAA35512.1.
PIRiS03651. RDECD7.
RefSeqiNP_065309.1. NC_002525.1.
WP_000777554.1. NZ_LXIU01000334.1.
YP_004422906.1. NC_015472.1.
YP_190212.1. NC_006671.1.

Genome annotation databases

GeneIDi10549022.
1238688.
3244885.
KEGGiag:CAA25445.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X00926 Genomic DNA. Translation: CAA25445.1.
X17477 Genomic DNA. Translation: CAA35509.1.
X17478 Genomic DNA. Translation: CAA35512.1.
PIRiS03651. RDECD7.
RefSeqiNP_065309.1. NC_002525.1.
WP_000777554.1. NZ_LXIU01000334.1.
YP_004422906.1. NC_015472.1.
YP_190212.1. NC_006671.1.

3D structure databases

ProteinModelPortaliP00382.
SMRiP00382.
ModBaseiSearch...
MobiDBiSearch...

Chemistry databases

BindingDBiP00382.
ChEMBLiCHEMBL2627.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi10549022.
1238688.
3244885.
KEGGiag:CAA25445.

Phylogenomic databases

KOiK18589.

Enzyme and pathway databases

UniPathwayiUPA00077; UER00158.

Miscellaneous databases

PROiP00382.

Family and domain databases

CDDicd00209. DHFR. 1 hit.
Gene3Di3.40.430.10. 1 hit.
InterProiIPR012259. DHFR.
IPR024072. DHFR-like_dom.
IPR017925. DHFR_CS.
IPR001796. DHFR_dom.
[Graphical view]
PfamiPF00186. DHFR_1. 1 hit.
[Graphical view]
PRINTSiPR00070. DHFR.
SUPFAMiSSF53597. SSF53597. 1 hit.
PROSITEiPS00075. DHFR_1. 1 hit.
PS51330. DHFR_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiDYR1_ECOLX
AccessioniPrimary (citable) accession number: P00382
Secondary accession number(s): P13923
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: July 21, 1986
Last modified: November 30, 2016
This is version 102 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Direct protein sequencing, Plasmid, Transposable element

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.