P00380 (DYR_ENTFC) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Dihydrofolate reductase
EC=1.5.1.3

Gene names

Name:

folA

Organism

Enterococcus faecium (Streptococcus faecium)

Taxonomic identifier

1352 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Lactobacillales › Enterococcaceae › Enterococcus

Protein attributes

Sequence length	167 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Key enzyme in folate metabolism. Catalyzes an essential reaction for de novo glycine and purine synthesis, and for DNA precursor synthesis By similarity.
Catalytic activity	5,6,7,8-tetrahydrofolate + NADP+ = 7,8-dihydrofolate + NADPH.
Pathway	Cofactor biosynthesis; tetrahydrofolate biosynthesis; 5,6,7,8-tetrahydrofolate from 7,8-dihydrofolate: step 1/1.
Sequence similarities	Belongs to the dihydrofolate reductase family. Contains 1 DHFR (dihydrofolate reductase) domain.

Ontologies

Keywords
Biological process	One-carbon metabolism
Ligand	NADP
Molecular function	Oxidoreductase
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	glycine biosynthetic process Inferred from electronic annotation. Source: InterPro nucleotide biosynthetic process Inferred from electronic annotation. Source: InterPro one-carbon metabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	NADP binding Inferred from electronic annotation. Source: InterPro dihydrofolate reductase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 167	167	Dihydrofolate reductase		PRO_0000186390
Regions
	Domain	1 – 162	162	DHFR
	Nucleotide binding	13 – 19	7	NADP By similarity
	Nucleotide binding	45 – 46	2	NADP By similarity
	Nucleotide binding	64 – 65	2	NADP By similarity
	Nucleotide binding	99 – 106	8	NADP By similarity
Sites
	Binding site	7	1	NADP; via amide nitrogen and carbonyl oxygen By similarity
	Binding site	27	1	Substrate By similarity
	Binding site	58	1	Substrate By similarity
	Binding site	117	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	P00380 [UniParc]. Last modified July 21, 1986. Version 1. Checksum: E00C2CF3BC420585	FASTA	167	19,583
	10 20 30 40 50 60 MFISMWAQDK NGLIGKDGLL PWRLPNDMRF FREHTMDKIL VMGRKTYEGM GKLSLPYRHI 70 80 90 100 110 120 IVLTTQKDFK VEKNAEVLHS IDELLAYAKD IPEDIYVSGG SRIFQALLPE TKIIWRTLID 130 140 150 160 AEFEGDTFIG EIDFTSFELV EEHEGIVNQE NQYPHRFQKW QKMSKVV

References

[1]	"The structure of the mutant dihydrofolate reductase from Streptococcus faecium. Amino acid sequence of peptide CNBr 7 and complete sequence of the protein." Peterson D.L., Gleisner J.M., Blakley R.L. J. Biol. Chem. 250:4945-4954(1975) [PubMed: 1097435] [Abstract] Cited for: PROTEIN SEQUENCE. Strain: A / var. Durans.
+	Additional computationally mapped references.

Cross-references

Sequence databases
PIR	RDSODF. A00392.
3D structure databases
ProteinModelPortal	P00380.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR012259. DHFR. IPR024072. DHFR-like_dom. IPR017925. DHFR_CS. IPR001796. DHFR_dom. [Graphical view]
Gene3D	G3DSA:3.40.430.10. G3DSA:3.40.430.10. 1 hit.
PANTHER	PTHR11549:SF1. DHFR. 1 hit.
Pfam	PF00186. DHFR_1. 1 hit. [Graphical view]
PIRSF	PIRSF000194. DHFR. 1 hit.
PRINTS	PR00070. DHFR.
SUPFAM	SSF53597. SSF53597. 1 hit.
PROSITE	PS00075. DHFR_1. 1 hit. PS51330. DHFR_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DYR_ENTFC

Accession

Primary (citable) accession number: P00380

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 21, 1986
Last sequence update:	July 21, 1986
Last modified:	January 25, 2012
This is version 64 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program