Reviewed, UniProtKB/Swiss-Prot P00376 (DYR_BOVIN)

Names and origin

Protein names

Recommended name:
    Dihydrofolate reductase
    EC=1.5.1.3

Gene names

Name:

DHFR

Organism

Bos taurus (Bovine)

Taxonomic identifier

9913 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Cetartiodactyla › Ruminantia › Pecora › Bovidae › Bovinae › Bos

Protein attributes

Sequence length	187 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Catalytic activity	5,6,7,8-tetrahydrofolate + NADP+ = 7,8-dihydrofolate + NADPH.
Pathway	Cofactor biosynthesis; tetrahydrofolate biosynthesis; tetrahydrofolate from dihydrofolate: step 1/1.
Miscellaneous	The reaction catalyzed by this enzyme represents an essential step for de novo glycine and purine synthesis, DNA precursor synthesis, and for the conversion of dUMP to dTMP.
Sequence similarities	Belongs to the dihydrofolate reductase family. Contains 1 DHFR (dihydrofolate reductase) domain.

Ontologies

Keywords
Biological process	One-carbon metabolism
Ligand	NADP
Molecular function	Oxidoreductase
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	glycine biosynthetic process Inferred from electronic annotation. Source: InterPro nucleotide biosynthetic process Inferred from electronic annotation. Source: InterPro one-carbon compound metabolic process Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	NADP or NADPH binding Inferred from electronic annotation. Source: InterPro dihydrofolate reductase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed Ref.2
	Chain	2 – 187	186	Dihydrofolate reductase		PRO_0000186361
Regions
	Domain	4 – 185	182	DHFR
Experimental info
	Sequence conflict	22	1	D → N in AAI14163. Ref.1
	Sequence conflict	102	1	E → Q AA sequence Ref.2

Sequences

	Sequence		Length	Mass (Da)
	P00376-1 [UniParc]. Last modified January 23, 2007. Version 3. Checksum: 0727DD855C8A7CD8	FASTA	187	21,604
	10 20 30 40 50 60 MVRPLNCIVA VSQNMGIGKN GDLPWPPLRN EFQYFQRMTT VSSVEGKQNL VIMGRKTWFS 70 80 90 100 110 120 IPEKNRPLKD RINIVLSREL KEPPKGAHFL AKSLDDALEL IEDPELTNKV DVVWIVGGSS 130 140 150 160 170 180 VYKEAMNKPG HVRLFVTRIM QEFESDAFFP EIDFEKYKLL PEYPGVPLDV QEEKGIKYKF EVYEKNN

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	NIH - Mammalian Gene Collection (MGC) project Submitted (FEB-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: Hereford. Tissue: Heart ventricle.
[2]	"Structure of dihydrofolate reductase: primary sequence of the bovine liver enzyme." Lai P.-H., Pan Y.-C.E., Gleisner J.M., Peterson D.L., Williams K.R., Blakley R.L. Biochemistry 21:3284-3294(1982) [PubMed: 7115669] [Abstract] Cited for: PROTEIN SEQUENCE OF 2-187. Tissue: Liver.

Cross-references

Sequence databases
EMBLGenBankDDBJ	BC114162 mRNA. Translation: AAI14163.1.
IPI	IPI00742596.
PIR	RDBOD. A00388.
RefSeq	NP_001071351.1.
UniGene	Bt.18730
3D structure databases
HSSP	HSSP built from PDB template 1DHF based on UniProtKB P00374.
SMR	P00376. Positions 2-187.
ModBase	Search...
Genome annotation databases
Ensembl	ENSBTAG00000007681. Bos taurus. [Contig view]
GeneID	508809.
KEGG	bta:508809.
Phylogenomic databases
HOVERGEN	P00376.
Enzyme and pathway databases
BRENDA	1.5.1.3. 251.
Family and domain databases
InterPro	IPR012259. DHFR. IPR001796. DHFR_reg. IPR017925. Dihydrofolate_reductase_CS. [Graphical view]
Pfam	PF00186. DHFR_1. 1 hit. [Graphical view]
PRINTS	PR00070. DHFR.
PROSITE	PS00075. DHFR_1. 1 hit. PS51330. DHFR_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DYR_BOVIN

Accession

Primary (citable) accession number: P00376
Secondary accession number(s): Q29RI1

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 21, 1986
Last sequence update:	January 23, 2007
Last modified:	June 16, 2009
This is version 60 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)