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P00376 (DYR_BOVIN) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 94. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Dihydrofolate reductase

EC=1.5.1.3
Gene names
Name:DHFR
OrganismBos taurus (Bovine) [Reference proteome]
Taxonomic identifier9913 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos

Protein attributes

Sequence length187 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Key enzyme in folate metabolism. Contributes to the de novo mitochondrial thymidylate biosynthesis pathway. Catalyzes an essential reaction for de novo glycine and purine synthesis, and for DNA precursor synthesis. Binds its own mRNA and that of DHFRL1 By similarity.

Catalytic activity

5,6,7,8-tetrahydrofolate + NADP+ = 7,8-dihydrofolate + NADPH.

Pathway

Cofactor biosynthesis; tetrahydrofolate biosynthesis; 5,6,7,8-tetrahydrofolate from 7,8-dihydrofolate: step 1/1.

Subunit structure

Homodimer By similarity.

Sequence similarities

Belongs to the dihydrofolate reductase family.

Contains 1 DHFR (dihydrofolate reductase) domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed Ref.2
Chain2 – 187186Dihydrofolate reductase
PRO_0000186361

Regions

Domain4 – 185182DHFR
Nucleotide binding16 – 227NADP By similarity
Nucleotide binding55 – 573NADP By similarity
Nucleotide binding77 – 793NADP By similarity
Nucleotide binding117 – 1248NADP By similarity
Region31 – 366Substrate binding By similarity

Sites

Binding site101NADP; via amide nitrogen and carbonyl oxygen By similarity
Binding site711Substrate By similarity

Experimental info

Sequence conflict221D → N in AAI14163. Ref.1
Sequence conflict1021E → Q AA sequence Ref.2

Sequences

Sequence LengthMass (Da)Tools
P00376 [UniParc].

Last modified January 23, 2007. Version 3.
Checksum: 0727DD855C8A7CD8

FASTA18721,604
        10         20         30         40         50         60 
MVRPLNCIVA VSQNMGIGKN GDLPWPPLRN EFQYFQRMTT VSSVEGKQNL VIMGRKTWFS 

        70         80         90        100        110        120 
IPEKNRPLKD RINIVLSREL KEPPKGAHFL AKSLDDALEL IEDPELTNKV DVVWIVGGSS 

       130        140        150        160        170        180 
VYKEAMNKPG HVRLFVTRIM QEFESDAFFP EIDFEKYKLL PEYPGVPLDV QEEKGIKYKF 


EVYEKNN 

« Hide

References

« Hide 'large scale' references
[1]NIH - Mammalian Gene Collection (MGC) project
Submitted (FEB-2006) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: Hereford.
Tissue: Heart ventricle.
[2]"Structure of dihydrofolate reductase: primary sequence of the bovine liver enzyme."
Lai P.-H., Pan Y.-C.E., Gleisner J.M., Peterson D.L., Williams K.R., Blakley R.L.
Biochemistry 21:3284-3294(1982) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 2-187.
Tissue: Liver.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
BC114162 mRNA. Translation: AAI14163.1.
PIRRDBOD. A00388.
RefSeqNP_001071351.1. NM_001077883.1.
UniGeneBt.18730.

3D structure databases

ProteinModelPortalP00376.
SMRP00376. Positions 2-187.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING9913.ENSBTAP00000010103.

Chemistry

BindingDBP00376.
ChEMBLCHEMBL1075051.

Proteomic databases

PaxDbP00376.
PRIDEP00376.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSBTAT00000010103; ENSBTAP00000010103; ENSBTAG00000007681.
GeneID508809.
KEGGbta:508809.

Organism-specific databases

CTD1719.

Phylogenomic databases

eggNOGCOG0262.
GeneTreeENSGT00390000010283.
HOGENOMHOG000040235.
HOVERGENHBG000773.
InParanoidP00376.
KOK00287.
OrthoDBEOG7V1FS3.
TreeFamTF317636.

Enzyme and pathway databases

SABIO-RKP00376.
UniPathwayUPA00077; UER00158.

Family and domain databases

Gene3D3.40.430.10. 1 hit.
InterProIPR012259. DHFR.
IPR024072. DHFR-like_dom.
IPR017925. DHFR_CS.
IPR001796. DHFR_dom.
[Graphical view]
PfamPF00186. DHFR_1. 1 hit.
[Graphical view]
PRINTSPR00070. DHFR.
SUPFAMSSF53597. SSF53597. 1 hit.
PROSITEPS00075. DHFR_1. 1 hit.
PS51330. DHFR_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio20868689.

Entry information

Entry nameDYR_BOVIN
AccessionPrimary (citable) accession number: P00376
Secondary accession number(s): Q29RI1
Entry history
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: January 23, 2007
Last modified: February 19, 2014
This is version 94 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways