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Protein

D-amino-acid oxidase

Gene

DAO

Organism
Sus scrofa (Pig)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Regulates the level of the neuromodulator D-serine in the brain. Has high activity towards D-DOPA and contributes to dopamine synthesis. Could act as a detoxifying agent which removes D-amino acids accumulated during aging. Acts on a variety of D-amino acids with a preference for those having small hydrophobic side chains followed by those bearing polar, aromatic, and basic groups. Does not act on acidic amino acids.

Catalytic activityi

A D-amino acid + H2O + O2 = a 2-oxo acid + NH3 + H2O2.3 Publications

Cofactori

FAD1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei53 – 531Substrate
Binding sitei164 – 1641FAD; via amide nitrogen and carbonyl oxygen1 Publication
Binding sitei182 – 1821FAD1 Publication
Binding sitei217 – 2171Substrate
Binding sitei228 – 2281Substrate
Binding sitei283 – 2831Substrate
Binding sitei313 – 3131Substrate; via carbonyl oxygen
Binding sitei317 – 3171FAD1 Publication

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi3 – 1715FAD1 PublicationAdd
BLAST
Nucleotide bindingi37 – 382FAD1 Publication
Nucleotide bindingi44 – 452FAD1 Publication
Nucleotide bindingi49 – 513FAD1 Publication
Nucleotide bindingi312 – 3165FAD1 Publication

GO - Molecular functioni

  • D-amino-acid oxidase activity Source: UniProtKB
  • FAD binding Source: Ensembl

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Ligandi

FAD, Flavoprotein

Enzyme and pathway databases

BRENDAi1.4.3.3. 6170.
ReactomeiR-SSC-389661. Glyoxylate metabolism and glycine degradation.
SABIO-RKP00371.

Names & Taxonomyi

Protein namesi
Recommended name:
D-amino-acid oxidase (EC:1.4.3.3)
Short name:
DAAO
Short name:
DAMOX
Short name:
DAO
Gene namesi
Name:DAO
OrganismiSus scrofa (Pig)
Taxonomic identifieri9823 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaSuinaSuidaeSus
Proteomesi
  • UP000008227 Componenti: Chromosome 14

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Peroxisome

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi55 – 551Y → F: No effect. 1 Publication
Mutagenesisi110 – 1101M → L: No effect. 1 Publication
Mutagenesisi217 – 2171H → L: No effect. 1 Publication
Mutagenesisi228 – 2281Y → F: Reduces activity. 1 Publication
Mutagenesisi307 – 3071H → L: Reduces activity. 1 Publication

Chemistry

ChEMBLiCHEMBL6172.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 347347D-amino-acid oxidasePRO_0000162763Add
BLAST

Proteomic databases

PaxDbiP00371.
PeptideAtlasiP00371.

Miscellaneous databases

PMAP-CutDBP00371.

Expressioni

Gene expression databases

BgeeiENSSSCG00000009942.
GenevisibleiP00371. SS.

Interactioni

Subunit structurei

Homodimer.2 Publications

Protein-protein interaction databases

STRINGi9823.ENSSSCP00000010609.

Chemistry

BindingDBiP00371.

Structurei

Secondary structure

1
347
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi2 – 65Combined sources
Helixi10 – 2314Combined sources
Turni24 – 263Combined sources
Beta strandi27 – 293Combined sources
Beta strandi31 – 377Combined sources
Helixi40 – 423Combined sources
Helixi44 – 474Combined sources
Helixi63 – 7715Combined sources
Turni78 – 803Combined sources
Turni82 – 843Combined sources
Helixi85 – 873Combined sources
Beta strandi89 – 10012Combined sources
Turni106 – 1105Combined sources
Beta strandi111 – 1166Combined sources
Helixi119 – 1224Combined sources
Beta strandi125 – 1273Combined sources
Beta strandi129 – 13911Combined sources
Helixi141 – 15414Combined sources
Beta strandi158 – 1614Combined sources
Helixi167 – 1726Combined sources
Beta strandi176 – 1805Combined sources
Helixi183 – 1886Combined sources
Beta strandi196 – 20611Combined sources
Beta strandi212 – 2165Combined sources
Turni219 – 2213Combined sources
Turni222 – 2243Combined sources
Beta strandi228 – 2314Combined sources
Beta strandi233 – 2397Combined sources
Helixi253 – 26614Combined sources
Helixi268 – 2725Combined sources
Beta strandi274 – 28512Combined sources
Beta strandi290 – 2989Combined sources
Beta strandi301 – 3099Combined sources
Helixi312 – 3143Combined sources
Helixi315 – 33622Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1AN9X-ray2.50A/B1-340[»]
1DAOX-ray3.20A/B/C/D/E/F/G/H1-347[»]
1DDOX-ray3.10A/B/C/D/E/F/G/H1-347[»]
1EVIX-ray2.50A/B1-340[»]
1KIFX-ray2.60A/B/C/D/E/F/G/H1-347[»]
1VE9X-ray2.50A/B1-347[»]
3WGTX-ray1.88A/B1-347[»]
4YJDX-ray2.30A/B1-340[»]
4YJFX-ray2.20A1-341[»]
B1-339[»]
4YJGX-ray2.50A1-341[»]
B1-340[»]
4YJHX-ray2.70A/B1-340[»]
ProteinModelPortaliP00371.
SMRiP00371. Positions 1-340.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP00371.

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi345 – 3473Microbody targeting signal

Sequence similaritiesi

Belongs to the DAMOX/DASOX family.Curated

Phylogenomic databases

eggNOGiKOG3923. Eukaryota.
COG0665. LUCA.
GeneTreeiENSGT00390000018635.
HOGENOMiHOG000046303.
HOVERGENiHBG003493.
InParanoidiP00371.
KOiK00273.
OMAiRARCIHN.
OrthoDBiEOG091G0G0Y.
TreeFamiTF313887.

Family and domain databases

Gene3Di3.40.50.720. 3 hits.
InterProiIPR006181. D-amino_acid_oxidase_CS.
IPR023209. DAO.
IPR006076. FAD-dep_OxRdtase.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PANTHERiPTHR11530. PTHR11530. 1 hit.
PfamiPF01266. DAO. 1 hit.
[Graphical view]
PIRSFiPIRSF000189. D-aa_oxidase. 1 hit.
PROSITEiPS00677. DAO. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P00371-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRVVVIGAGV IGLSTALCIH ERYHSVLQPL DVKVYADRFT PFTTTDVAAG
60 70 80 90 100
LWQPYTSEPS NPQEANWNQQ TFNYLLSHIG SPNAANMGLT PVSGYNLFRE
110 120 130 140 150
AVPDPYWKDM VLGFRKLTPR ELDMFPDYRY GWFNTSLILE GRKYLQWLTE
160 170 180 190 200
RLTERGVKFF LRKVESFEEV ARGGADVIIN CTGVWAGVLQ PDPLLQPGRG
210 220 230 240 250
QIIKVDAPWL KNFIITHDLE RGIYNSPYII PGLQAVTLGG TFQVGNWNEI
260 270 280 290 300
NNIQDHNTIW EGCCRLEPTL KDAKIVGEYT GFRPVRPQVR LEREQLRFGS
310 320 330 340
SNTEVIHNYG HGGYGLTIHW GCALEVAKLF GKVLEERNLL TMPPSHL
Length:347
Mass (Da):39,336
Last modified:July 1, 1989 - v2
Checksum:i0EC6577BDB2BF46C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M16972 mRNA. Translation: AAA30985.1.
M18447
, M18444, M18445, M18446 Genomic DNA. Translation: AAA31025.1.
M18448 mRNA. Translation: AAA31026.1.
PIRiA29598. OXPGDA.
A33798.
RefSeqiNP_999231.1. NM_214066.2.
UniGeneiSsc.232.

Genome annotation databases

EnsembliENSSSCT00000010894; ENSSSCP00000010609; ENSSSCG00000009942.
GeneIDi397134.
KEGGissc:397134.

Cross-referencesi

Web resourcesi

Worthington enzyme manual

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M16972 mRNA. Translation: AAA30985.1.
M18447
, M18444, M18445, M18446 Genomic DNA. Translation: AAA31025.1.
M18448 mRNA. Translation: AAA31026.1.
PIRiA29598. OXPGDA.
A33798.
RefSeqiNP_999231.1. NM_214066.2.
UniGeneiSsc.232.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1AN9X-ray2.50A/B1-340[»]
1DAOX-ray3.20A/B/C/D/E/F/G/H1-347[»]
1DDOX-ray3.10A/B/C/D/E/F/G/H1-347[»]
1EVIX-ray2.50A/B1-340[»]
1KIFX-ray2.60A/B/C/D/E/F/G/H1-347[»]
1VE9X-ray2.50A/B1-347[»]
3WGTX-ray1.88A/B1-347[»]
4YJDX-ray2.30A/B1-340[»]
4YJFX-ray2.20A1-341[»]
B1-339[»]
4YJGX-ray2.50A1-341[»]
B1-340[»]
4YJHX-ray2.70A/B1-340[»]
ProteinModelPortaliP00371.
SMRiP00371. Positions 1-340.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9823.ENSSSCP00000010609.

Chemistry

BindingDBiP00371.
ChEMBLiCHEMBL6172.

Proteomic databases

PaxDbiP00371.
PeptideAtlasiP00371.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSSSCT00000010894; ENSSSCP00000010609; ENSSSCG00000009942.
GeneIDi397134.
KEGGissc:397134.

Organism-specific databases

CTDi1610.

Phylogenomic databases

eggNOGiKOG3923. Eukaryota.
COG0665. LUCA.
GeneTreeiENSGT00390000018635.
HOGENOMiHOG000046303.
HOVERGENiHBG003493.
InParanoidiP00371.
KOiK00273.
OMAiRARCIHN.
OrthoDBiEOG091G0G0Y.
TreeFamiTF313887.

Enzyme and pathway databases

BRENDAi1.4.3.3. 6170.
ReactomeiR-SSC-389661. Glyoxylate metabolism and glycine degradation.
SABIO-RKP00371.

Miscellaneous databases

EvolutionaryTraceiP00371.
PMAP-CutDBP00371.
PROiP00371.

Gene expression databases

BgeeiENSSSCG00000009942.
GenevisibleiP00371. SS.

Family and domain databases

Gene3Di3.40.50.720. 3 hits.
InterProiIPR006181. D-amino_acid_oxidase_CS.
IPR023209. DAO.
IPR006076. FAD-dep_OxRdtase.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PANTHERiPTHR11530. PTHR11530. 1 hit.
PfamiPF01266. DAO. 1 hit.
[Graphical view]
PIRSFiPIRSF000189. D-aa_oxidase. 1 hit.
PROSITEiPS00677. DAO. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiOXDA_PIG
AccessioniPrimary (citable) accession number: P00371
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: July 1, 1989
Last modified: September 7, 2016
This is version 139 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.