Reviewed, UniProtKB/Swiss-Prot P00370 (DHE4_ECOLI)

Names and origin

Protein names

Recommended name:
    NADP-specific glutamate dehydrogenase
      Short name=NADP-GDH
    EC=1.4.1.4

Gene names

Name:	gdhA
Ordered Locus Names:	b1761, JW1750

Organism

Escherichia coli (strain K12) [Complete proteome] [HAMAP]

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	447 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Catalytic activity	L-glutamate + H2O + NADP+ = 2-oxoglutarate + NH3 + NADPH.
Subunit structure	Homohexamer.
Sequence similarities	Belongs to the Glu/Leu/Phe/Val dehydrogenases family.

Ontologies

Keywords
Ligand	NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome Direct protein sequencing
Gene Ontology (GO)
Biological process	amino acid metabolic process Inferred from electronic annotation. Source: InterPro oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	binding Inferred from electronic annotation. Source: InterPro glutamate dehydrogenase (NADP+) activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 447	447	NADP-specific glutamate dehydrogenase		PRO_0000182769
Sites
	Active site	128	1	By similarity
Experimental info
	Sequence conflict	385	1	A → P in AAA23868. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	P00370-1 [UniParc]. Last modified July 21, 1986. Version 1. Checksum: 7CB861D282C533C6	FASTA	447	48,581
	10 20 30 40 50 60 MDQTYSLESF LNHVQKRDPN QTEFAQAVRE VMTTLWPFLE QNPKYRQMSL LERLVEPERV 70 80 90 100 110 120 IQFRVVWVDD RNQIQVNRAW RVQFSSAIGP YKGGMRFHPS VNLSILKFLG FEQTFKNALT 130 140 150 160 170 180 TLPMGGGKGG SDFDPKGKSE GEVMRFCQAL MTELYRHLGA DTDVPAGDIG VGGREVGFMA 190 200 210 220 230 240 GMMKKLSNNT ACVFTGKGLS FGGSLIRPEA TGYGLVYFTE AMLKRHGMGF EGMRVSVSGS 250 260 270 280 290 300 GNVAQYAIEK AMEFGARVIT ASDSSGTVVD ESGFTKEKLA RLIEIKASRD GRVADYAKEF 310 320 330 340 350 360 GLVYLEGQQP WSLPVDIALP CATQNELDVD AAHQLIANGV KAVAEGANMP TTIEATELFQ 370 380 390 400 410 420 QAGVLFAPGK AANAGGVATS GLEMAQNAAR LGWKAEKVDA RLHHIMLDIH HACVEHGGEG 430 440 EQTNYVQGAN IAGFVKVADA MLAQGVI

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Complete nucleotide sequence of the Escherichia coli gdhA gene." McPherson M.J., Wootton J.C. Nucleic Acids Res. 11:5257-5266(1983) [PubMed: 6308576] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 1-36. Strain: K12.
[2]	"Complete nucleotide sequence of the glutamate dehydrogenase gene from Escherichia coli K-12." Valle F., Becerril B., Chen E., Seeburg P.H., Heyneker H., Bolivar F. Gene 27:193-199(1984) [PubMed: 6373501] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"A 570-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 28.0-40.1 min region on the linkage map." Aiba H., Baba T., Fujita K., Hayashi K., Inada T., Isono K., Itoh T., Kasai H., Kashimoto K., Kimura S., Kitakawa M., Kitagawa M., Makino K., Miki T., Mizobuchi K., Mori H., Mori T., Motomura K. , Nakade S., Nakamura Y., Nashimoto H., Nishio Y., Oshima T., Saito N., Sampei G., Seki Y., Sivasundaram S., Tagami H., Takeda J., Takemoto K., Takeuchi Y., Wada C., Yamamoto Y., Horiuchi T. DNA Res. 3:363-377(1996) [PubMed: 9097039] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[4]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[5]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[6]	"Comparing the predicted and observed properties of proteins encoded in the genome of Escherichia coli K-12." Link A.J., Robison K., Church G.M. Electrophoresis 18:1259-1313(1997) [PubMed: 9298646] [Abstract] Cited for: PROTEIN SEQUENCE OF 1-12. Strain: K12 / EMG2.

Cross-references

Sequence databases
EMBLGenBankDDBJ	J01615 Genomic DNA. Translation: AAA87979.1. K02499 Genomic DNA. Translation: AAA23868.1. U00096 Genomic DNA. Translation: AAC74831.1. AP009048 Genomic DNA. Translation: BAA15550.1.
PIR	DEECEN. A00382.
RefSeq	AP_002380.1. NP_416275.1.
3D structure databases
HSSP	HSSP built from PDB template 1AUP based on UniProtKB P24295.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP:9756N.
2-D gel databases
SWISS-2DPAGE	P00370.
ECO2DBASE	G043.6. 6TH EDITION. G043.7. 6TH EDITION.
Genome annotation databases
GeneID	946802.
GenomeReviews	Gene locus JW1750 in contig AP009048_GR. Gene locus b1761 in contig U00096_GR.
KEGG	ecj:JW1750. eco:b1761.
Organism-specific databases
EchoBASE	EB0367.
EcoGene	EG10372. gdhA.
CMR	Search...
Phylogenomic databases
HOGENOM	P00370.
OMA	P00370. EMAQNAS.
Enzyme and pathway databases
BioCyc	EcoCyc:GDHA-MON. MetaCyc:GDHA-MON.
Family and domain databases
InterPro	IPR006095. Glu/Leu/Phe/Val_DH. IPR006096. Glu/Leu/Phe/Val_DH_C. IPR006097. Glu/Leu/Phe/Val_DH_dimer. IPR014362. Glu_DH. IPR016040. NAD(P)-bd_dom. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
PANTHER	PTHR11606:SF2. GLFV_DH. 1 hit.
Pfam	PF00208. ELFV_dehydrog. 1 hit. PF02812. ELFV_dehydrog_N. 1 hit. [Graphical view]
PIRSF	PIRSF000185. Glu_DH. 1 hit.
PRINTS	PR00082. GLFDHDRGNASE.
PROSITE	PS00074. GLFV_DEHYDROGENASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DHE4_ECOLI

Accession

Primary (citable) accession number: P00370
Secondary accession number(s): P78173

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 21, 1986
Last sequence update:	July 21, 1986
Last modified:	June 16, 2009
This is version 77 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)