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P00369

- DHE4_NEUCR

UniProt

P00369 - DHE4_NEUCR

Protein

NADP-specific glutamate dehydrogenase

Gene

gdh

Organism
Neurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 115 (01 Oct 2014)
      Sequence version 3 (23 Jan 2007)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    L-glutamate + H2O + NADP+ = 2-oxoglutarate + NH3 + NADPH.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei114 – 1141PROSITE-ProRule annotation

    GO - Molecular functioni

    1. glutamate dehydrogenase (NADP+) activity Source: UniProtKB-EC

    GO - Biological processi

    1. ammonia assimilation cycle Source: EnsemblFungi
    2. glutamate biosynthetic process Source: EnsemblFungi

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Ligandi

    NADP

    Enzyme and pathway databases

    BioCyciMetaCyc:MONOMER-13461.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    NADP-specific glutamate dehydrogenase (EC:1.4.1.4)
    Short name:
    NADP-GDH
    Alternative name(s):
    NADP-dependent glutamate dehydrogenase
    Gene namesi
    Name:gdh
    Synonyms:am
    ORF Names:18F11.230, NCU01195
    OrganismiNeurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987)
    Taxonomic identifieri367110 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaPezizomycotinaSordariomycetesSordariomycetidaeSordarialesSordariaceaeNeurospora
    ProteomesiUP000001805: Chromosome 2, Linkage Group V

    Subcellular locationi

    GO - Cellular componenti

    1. mitochondrion Source: EnsemblFungi
    2. nucleus Source: EnsemblFungi

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11Removed1 Publication
    Chaini2 – 454453NADP-specific glutamate dehydrogenasePRO_0000182790Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei2 – 21N-acetylserine1 Publication

    Keywords - PTMi

    Acetylation

    Proteomic databases

    PRIDEiP00369.

    Interactioni

    Subunit structurei

    Homohexamer.

    Protein-protein interaction databases

    STRINGi5141.NCU01195.1.

    Structurei

    3D structure databases

    ProteinModelPortaliP00369.
    SMRiP00369. Positions 3-450.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiCOG0334.
    HOGENOMiHOG000243799.
    KOiK00262.
    OMAiIMLDIHA.
    OrthoDBiEOG7XSTPW.

    Family and domain databases

    Gene3Di3.40.50.720. 1 hit.
    InterProiIPR006095. Glu/Leu/Phe/Val_DH.
    IPR006096. Glu/Leu/Phe/Val_DH_C.
    IPR006097. Glu/Leu/Phe/Val_DH_dimer_dom.
    IPR014362. Glu_DH.
    IPR016040. NAD(P)-bd_dom.
    [Graphical view]
    PfamiPF00208. ELFV_dehydrog. 1 hit.
    PF02812. ELFV_dehydrog_N. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000185. Glu_DH. 1 hit.
    PRINTSiPR00082. GLFDHDRGNASE.
    SMARTiSM00839. ELFV_dehydrog. 1 hit.
    [Graphical view]
    PROSITEiPS00074. GLFV_DEHYDROGENASE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P00369-1 [UniParc]FASTAAdd to Basket

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    MSNLPSEPEF EQAYKELAYT LENSSLFQKH PEYRTALTVA SIPERVIQFR    50
    VVWEDDNGNV QVNRGYRVQF NSALGPYKGG LRLHPSVNLS ILKFLGFEQI 100
    FKNALTGLSM GGGKGGADFD PKGKSDAEIR RFCCAFMAEL HKHIGADTDV 150
    PAGDIGVGGR EIGYMFGAYR KAANRFEGVL TGKGLSWGGS LIRPEATGYG 200
    LVYYVGHMLE YSGAGSYAGK RVALSGSGNV AQYAALKLIE LGATVVSLSD 250
    SKGALVATGE SGITVEDINA VMAIKEARQS LTSFQHAGHL KWIEGARPWL 300
    HVGKVDIALP CATQNEVSKE EAEGLLAAGC KFVAEGSNMG CTLEAIEVFE 350
    NNRKEKKGEA VWYAPGKAAN CGGVAVSGLE MAQNSQRLNW TQAEVDEKLK 400
    DIMKNAFFNG LNTAKTYVEA AEGELPSLVA GSNIAGFVKV AQAMHDQGDW 450
    WSKN 454
    Length:454
    Mass (Da):48,853
    Last modified:January 23, 2007 - v3
    Checksum:i908B56A63837DA28
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti57 – 571N → D AA sequence (PubMed:1002)Curated
    Sequence conflicti314 – 3152QN → ED AA sequence (PubMed:1002)Curated
    Sequence conflicti451 – 4511Missing AA sequence (PubMed:1002)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    K01409 Genomic DNA. Translation: AAA33558.1.
    AL670011 Genomic DNA. Translation: CAD21426.1.
    CM002240 Genomic DNA. Translation: EAA32325.1.
    K01653 Genomic DNA. Translation: AAA33557.1.
    K02468 Genomic DNA. Translation: AAA33559.1.
    PIRiA91506. DENCEN.
    RefSeqiXP_961561.1. XM_956468.2.
    UniGeneiNcr.24998.

    Genome annotation databases

    EnsemblFungiiEFNCRT00000004414; EFNCRP00000004414; EFNCRG00000004408.
    GeneIDi3877684.
    KEGGincr:NCU01195.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    K01409 Genomic DNA. Translation: AAA33558.1 .
    AL670011 Genomic DNA. Translation: CAD21426.1 .
    CM002240 Genomic DNA. Translation: EAA32325.1 .
    K01653 Genomic DNA. Translation: AAA33557.1 .
    K02468 Genomic DNA. Translation: AAA33559.1 .
    PIRi A91506. DENCEN.
    RefSeqi XP_961561.1. XM_956468.2.
    UniGenei Ncr.24998.

    3D structure databases

    ProteinModelPortali P00369.
    SMRi P00369. Positions 3-450.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 5141.NCU01195.1.

    Proteomic databases

    PRIDEi P00369.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii EFNCRT00000004414 ; EFNCRP00000004414 ; EFNCRG00000004408 .
    GeneIDi 3877684.
    KEGGi ncr:NCU01195.

    Phylogenomic databases

    eggNOGi COG0334.
    HOGENOMi HOG000243799.
    KOi K00262.
    OMAi IMLDIHA.
    OrthoDBi EOG7XSTPW.

    Enzyme and pathway databases

    BioCyci MetaCyc:MONOMER-13461.

    Family and domain databases

    Gene3Di 3.40.50.720. 1 hit.
    InterProi IPR006095. Glu/Leu/Phe/Val_DH.
    IPR006096. Glu/Leu/Phe/Val_DH_C.
    IPR006097. Glu/Leu/Phe/Val_DH_dimer_dom.
    IPR014362. Glu_DH.
    IPR016040. NAD(P)-bd_dom.
    [Graphical view ]
    Pfami PF00208. ELFV_dehydrog. 1 hit.
    PF02812. ELFV_dehydrog_N. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF000185. Glu_DH. 1 hit.
    PRINTSi PR00082. GLFDHDRGNASE.
    SMARTi SM00839. ELFV_dehydrog. 1 hit.
    [Graphical view ]
    PROSITEi PS00074. GLFV_DEHYDROGENASE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The complete nucleotide sequence of the Neurospora crassa am (NADP-specific glutamate dehydrogenase) gene."
      Kinnaird J.H., Fincham J.R.S.
      Gene 26:253-260(1983) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. "What's in the genome of a filamentous fungus? Analysis of the Neurospora genome sequence."
      Mannhaupt G., Montrone C., Haase D., Mewes H.-W., Aign V., Hoheisel J.D., Fartmann B., Nyakatura G., Kempken F., Maier J., Schulte U.
      Nucleic Acids Res. 31:1944-1954(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987.
    3. "The genome sequence of the filamentous fungus Neurospora crassa."
      Galagan J.E., Calvo S.E., Borkovich K.A., Selker E.U., Read N.D., Jaffe D.B., FitzHugh W., Ma L.-J., Smirnov S., Purcell S., Rehman B., Elkins T., Engels R., Wang S., Nielsen C.B., Butler J., Endrizzi M., Qui D.
      , Ianakiev P., Bell-Pedersen D., Nelson M.A., Werner-Washburne M., Selitrennikoff C.P., Kinsey J.A., Braun E.L., Zelter A., Schulte U., Kothe G.O., Jedd G., Mewes H.-W., Staben C., Marcotte E., Greenberg D., Roy A., Foley K., Naylor J., Stange-Thomann N., Barrett R., Gnerre S., Kamal M., Kamvysselis M., Mauceli E.W., Bielke C., Rudd S., Frishman D., Krystofova S., Rasmussen C., Metzenberg R.L., Perkins D.D., Kroken S., Cogoni C., Macino G., Catcheside D.E.A., Li W., Pratt R.J., Osmani S.A., DeSouza C.P.C., Glass N.L., Orbach M.J., Berglund J.A., Voelker R., Yarden O., Plamann M., Seiler S., Dunlap J.C., Radford A., Aramayo R., Natvig D.O., Alex L.A., Mannhaupt G., Ebbole D.J., Freitag M., Paulsen I., Sachs M.S., Lander E.S., Nusbaum C., Birren B.W.
      Nature 422:859-868(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987.
    4. "The amino acid sequence of Neurospora NADP-specific glutamate dehydrogenase. Peptic and chymotryptic peptides and the complete sequence."
      Holder A.A., Wootton J.C., Baron A.J., Chambers G.K., Fincham J.R.S.
      Biochem. J. 149:757-773(1975) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 2-454.
    5. "Cloning of the am (glutamate dehydrogenase) gene of Neurospora crassa through the use of a synthetic DNA probe."
      Kinnaird J.H., Keighren M.A., Kinsey J.A., Eaton M., Fincham J.R.S.
      Gene 20:387-396(1982) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-23.
    6. "An unstable mutant gene of the am locus of Neurospora results from a small duplication."
      Rambosek J.A., Kinsey J.A.
      Gene 27:101-107(1984) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-25.

    Entry informationi

    Entry nameiDHE4_NEUCR
    AccessioniPrimary (citable) accession number: P00369
    Secondary accession number(s): Q7RVE1
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 21, 1986
    Last sequence update: January 23, 2007
    Last modified: October 1, 2014
    This is version 115 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3