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P00369 (DHE4_NEUCR) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 113. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
NADP-specific glutamate dehydrogenase

Short name=NADP-GDH
EC=1.4.1.4
Alternative name(s):
NADP-dependent glutamate dehydrogenase
Gene names
Name:gdh
Synonyms:am
ORF Names:18F11.230, NCU01195
OrganismNeurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987) [Reference proteome]
Taxonomic identifier367110 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaPezizomycotinaSordariomycetesSordariomycetidaeSordarialesSordariaceaeNeurospora

Protein attributes

Sequence length454 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Catalytic activity

L-glutamate + H2O + NADP+ = 2-oxoglutarate + NH3 + NADPH.

Subunit structure

Homohexamer.

Sequence similarities

Belongs to the Glu/Leu/Phe/Val dehydrogenases family.

Ontologies

Keywords
   LigandNADP
   Molecular functionOxidoreductase
   PTMAcetylation
   Technical termComplete proteome
Direct protein sequencing
Reference proteome
Gene Ontology (GO)
   Biological_processammonia assimilation cycle

Inferred from electronic annotation. Source: EnsemblFungi

glutamate biosynthetic process

Inferred from electronic annotation. Source: EnsemblFungi

   Cellular_componentmitochondrion

Inferred from electronic annotation. Source: EnsemblFungi

nucleus

Inferred from electronic annotation. Source: EnsemblFungi

   Molecular_functionglutamate dehydrogenase (NADP+) activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed Ref.4
Chain2 – 454453NADP-specific glutamate dehydrogenase
PRO_0000182790

Sites

Active site1141 By similarity

Amino acid modifications

Modified residue21N-acetylserine Ref.4

Experimental info

Sequence conflict571N → D AA sequence Ref.4
Sequence conflict314 – 3152QN → ED AA sequence Ref.4
Sequence conflict4511Missing AA sequence Ref.4

Sequences

Sequence LengthMass (Da)Tools
P00369 [UniParc].

Last modified January 23, 2007. Version 3.
Checksum: 908B56A63837DA28

FASTA45448,853
        10         20         30         40         50         60 
MSNLPSEPEF EQAYKELAYT LENSSLFQKH PEYRTALTVA SIPERVIQFR VVWEDDNGNV 

        70         80         90        100        110        120 
QVNRGYRVQF NSALGPYKGG LRLHPSVNLS ILKFLGFEQI FKNALTGLSM GGGKGGADFD 

       130        140        150        160        170        180 
PKGKSDAEIR RFCCAFMAEL HKHIGADTDV PAGDIGVGGR EIGYMFGAYR KAANRFEGVL 

       190        200        210        220        230        240 
TGKGLSWGGS LIRPEATGYG LVYYVGHMLE YSGAGSYAGK RVALSGSGNV AQYAALKLIE 

       250        260        270        280        290        300 
LGATVVSLSD SKGALVATGE SGITVEDINA VMAIKEARQS LTSFQHAGHL KWIEGARPWL 

       310        320        330        340        350        360 
HVGKVDIALP CATQNEVSKE EAEGLLAAGC KFVAEGSNMG CTLEAIEVFE NNRKEKKGEA 

       370        380        390        400        410        420 
VWYAPGKAAN CGGVAVSGLE MAQNSQRLNW TQAEVDEKLK DIMKNAFFNG LNTAKTYVEA 

       430        440        450 
AEGELPSLVA GSNIAGFVKV AQAMHDQGDW WSKN 

« Hide

References

« Hide 'large scale' references
[1]"The complete nucleotide sequence of the Neurospora crassa am (NADP-specific glutamate dehydrogenase) gene."
Kinnaird J.H., Fincham J.R.S.
Gene 26:253-260(1983) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"What's in the genome of a filamentous fungus? Analysis of the Neurospora genome sequence."
Mannhaupt G., Montrone C., Haase D., Mewes H.-W., Aign V., Hoheisel J.D., Fartmann B., Nyakatura G., Kempken F., Maier J., Schulte U.
Nucleic Acids Res. 31:1944-1954(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987.
[3]"The genome sequence of the filamentous fungus Neurospora crassa."
Galagan J.E., Calvo S.E., Borkovich K.A., Selker E.U., Read N.D., Jaffe D.B., FitzHugh W., Ma L.-J., Smirnov S., Purcell S., Rehman B., Elkins T., Engels R., Wang S., Nielsen C.B., Butler J., Endrizzi M., Qui D. expand/collapse author list , Ianakiev P., Bell-Pedersen D., Nelson M.A., Werner-Washburne M., Selitrennikoff C.P., Kinsey J.A., Braun E.L., Zelter A., Schulte U., Kothe G.O., Jedd G., Mewes H.-W., Staben C., Marcotte E., Greenberg D., Roy A., Foley K., Naylor J., Stange-Thomann N., Barrett R., Gnerre S., Kamal M., Kamvysselis M., Mauceli E.W., Bielke C., Rudd S., Frishman D., Krystofova S., Rasmussen C., Metzenberg R.L., Perkins D.D., Kroken S., Cogoni C., Macino G., Catcheside D.E.A., Li W., Pratt R.J., Osmani S.A., DeSouza C.P.C., Glass N.L., Orbach M.J., Berglund J.A., Voelker R., Yarden O., Plamann M., Seiler S., Dunlap J.C., Radford A., Aramayo R., Natvig D.O., Alex L.A., Mannhaupt G., Ebbole D.J., Freitag M., Paulsen I., Sachs M.S., Lander E.S., Nusbaum C., Birren B.W.
Nature 422:859-868(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987.
[4]"The amino acid sequence of Neurospora NADP-specific glutamate dehydrogenase. Peptic and chymotryptic peptides and the complete sequence."
Holder A.A., Wootton J.C., Baron A.J., Chambers G.K., Fincham J.R.S.
Biochem. J. 149:757-773(1975) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 2-454.
[5]"Cloning of the am (glutamate dehydrogenase) gene of Neurospora crassa through the use of a synthetic DNA probe."
Kinnaird J.H., Keighren M.A., Kinsey J.A., Eaton M., Fincham J.R.S.
Gene 20:387-396(1982) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-23.
[6]"An unstable mutant gene of the am locus of Neurospora results from a small duplication."
Rambosek J.A., Kinsey J.A.
Gene 27:101-107(1984) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-25.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
K01409 Genomic DNA. Translation: AAA33558.1.
AL670011 Genomic DNA. Translation: CAD21426.1.
CM002240 Genomic DNA. Translation: EAA32325.1.
K01653 Genomic DNA. Translation: AAA33557.1.
K02468 Genomic DNA. Translation: AAA33559.1.
PIRDENCEN. A91506.
RefSeqXP_961561.1. XM_956468.2.
UniGeneNcr.24998.

3D structure databases

ProteinModelPortalP00369.
SMRP00369. Positions 3-450.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING5141.NCU01195.1.

Proteomic databases

PRIDEP00369.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiEFNCRT00000004414; EFNCRP00000004414; EFNCRG00000004408.
GeneID3877684.
KEGGncr:NCU01195.

Phylogenomic databases

eggNOGCOG0334.
HOGENOMHOG000243799.
KOK00262.
OMAVAIYTIE.
OrthoDBEOG7XSTPW.

Enzyme and pathway databases

BioCycMetaCyc:MONOMER-13461.

Family and domain databases

Gene3D3.40.50.720. 1 hit.
InterProIPR006095. Glu/Leu/Phe/Val_DH.
IPR006096. Glu/Leu/Phe/Val_DH_C.
IPR006097. Glu/Leu/Phe/Val_DH_dimer_dom.
IPR014362. Glu_DH.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PfamPF00208. ELFV_dehydrog. 1 hit.
PF02812. ELFV_dehydrog_N. 1 hit.
[Graphical view]
PIRSFPIRSF000185. Glu_DH. 1 hit.
PRINTSPR00082. GLFDHDRGNASE.
SMARTSM00839. ELFV_dehydrog. 1 hit.
[Graphical view]
PROSITEPS00074. GLFV_DEHYDROGENASE. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameDHE4_NEUCR
AccessionPrimary (citable) accession number: P00369
Secondary accession number(s): Q7RVE1
Entry history
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: January 23, 2007
Last modified: February 19, 2014
This is version 113 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families