SubmitCancel

Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

P00369

- DHE4_NEUCR

UniProt

P00369 - DHE4_NEUCR

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein
NADP-specific glutamate dehydrogenase
Gene
gdh, am, 18F11.230, NCU01195
Organism
Neurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at protein leveli

Functioni

Catalytic activityi

L-glutamate + H2O + NADP+ = 2-oxoglutarate + NH3 + NADPH.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei114 – 1141 By similarity

GO - Molecular functioni

  1. glutamate dehydrogenase (NADP+) activity Source: UniProtKB-EC

GO - Biological processi

  1. ammonia assimilation cycle Source: EnsemblFungi
  2. glutamate biosynthetic process Source: EnsemblFungi
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Ligandi

NADP

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-13461.

Names & Taxonomyi

Protein namesi
Recommended name:
NADP-specific glutamate dehydrogenase (EC:1.4.1.4)
Short name:
NADP-GDH
Alternative name(s):
NADP-dependent glutamate dehydrogenase
Gene namesi
Name:gdh
Synonyms:am
ORF Names:18F11.230, NCU01195
OrganismiNeurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987)
Taxonomic identifieri367110 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaPezizomycotinaSordariomycetesSordariomycetidaeSordarialesSordariaceaeNeurospora
ProteomesiUP000001805: Chromosome 2, Linkage Group V

Subcellular locationi

GO - Cellular componenti

  1. mitochondrion Source: EnsemblFungi
  2. nucleus Source: EnsemblFungi
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed1 Publication
Chaini2 – 454453NADP-specific glutamate dehydrogenase
PRO_0000182790Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylserine1 Publication

Keywords - PTMi

Acetylation

Proteomic databases

PRIDEiP00369.

Interactioni

Subunit structurei

Homohexamer.

Protein-protein interaction databases

STRINGi5141.NCU01195.1.

Structurei

3D structure databases

ProteinModelPortaliP00369.
SMRiP00369. Positions 3-450.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0334.
HOGENOMiHOG000243799.
KOiK00262.
OMAiIMLDIHA.
OrthoDBiEOG7XSTPW.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
InterProiIPR006095. Glu/Leu/Phe/Val_DH.
IPR006096. Glu/Leu/Phe/Val_DH_C.
IPR006097. Glu/Leu/Phe/Val_DH_dimer_dom.
IPR014362. Glu_DH.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PfamiPF00208. ELFV_dehydrog. 1 hit.
PF02812. ELFV_dehydrog_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000185. Glu_DH. 1 hit.
PRINTSiPR00082. GLFDHDRGNASE.
SMARTiSM00839. ELFV_dehydrog. 1 hit.
[Graphical view]
PROSITEiPS00074. GLFV_DEHYDROGENASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P00369-1 [UniParc]FASTAAdd to Basket

« Hide

MSNLPSEPEF EQAYKELAYT LENSSLFQKH PEYRTALTVA SIPERVIQFR    50
VVWEDDNGNV QVNRGYRVQF NSALGPYKGG LRLHPSVNLS ILKFLGFEQI 100
FKNALTGLSM GGGKGGADFD PKGKSDAEIR RFCCAFMAEL HKHIGADTDV 150
PAGDIGVGGR EIGYMFGAYR KAANRFEGVL TGKGLSWGGS LIRPEATGYG 200
LVYYVGHMLE YSGAGSYAGK RVALSGSGNV AQYAALKLIE LGATVVSLSD 250
SKGALVATGE SGITVEDINA VMAIKEARQS LTSFQHAGHL KWIEGARPWL 300
HVGKVDIALP CATQNEVSKE EAEGLLAAGC KFVAEGSNMG CTLEAIEVFE 350
NNRKEKKGEA VWYAPGKAAN CGGVAVSGLE MAQNSQRLNW TQAEVDEKLK 400
DIMKNAFFNG LNTAKTYVEA AEGELPSLVA GSNIAGFVKV AQAMHDQGDW 450
WSKN 454
Length:454
Mass (Da):48,853
Last modified:January 23, 2007 - v3
Checksum:i908B56A63837DA28
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti57 – 571N → D AA sequence 1 Publication
Sequence conflicti314 – 3152QN → ED AA sequence 1 Publication
Sequence conflicti451 – 4511Missing AA sequence 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
K01409 Genomic DNA. Translation: AAA33558.1.
AL670011 Genomic DNA. Translation: CAD21426.1.
CM002240 Genomic DNA. Translation: EAA32325.1.
K01653 Genomic DNA. Translation: AAA33557.1.
K02468 Genomic DNA. Translation: AAA33559.1.
PIRiA91506. DENCEN.
RefSeqiXP_961561.1. XM_956468.2.
UniGeneiNcr.24998.

Genome annotation databases

EnsemblFungiiEFNCRT00000004414; EFNCRP00000004414; EFNCRG00000004408.
GeneIDi3877684.
KEGGincr:NCU01195.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
K01409 Genomic DNA. Translation: AAA33558.1 .
AL670011 Genomic DNA. Translation: CAD21426.1 .
CM002240 Genomic DNA. Translation: EAA32325.1 .
K01653 Genomic DNA. Translation: AAA33557.1 .
K02468 Genomic DNA. Translation: AAA33559.1 .
PIRi A91506. DENCEN.
RefSeqi XP_961561.1. XM_956468.2.
UniGenei Ncr.24998.

3D structure databases

ProteinModelPortali P00369.
SMRi P00369. Positions 3-450.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 5141.NCU01195.1.

Proteomic databases

PRIDEi P00369.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii EFNCRT00000004414 ; EFNCRP00000004414 ; EFNCRG00000004408 .
GeneIDi 3877684.
KEGGi ncr:NCU01195.

Phylogenomic databases

eggNOGi COG0334.
HOGENOMi HOG000243799.
KOi K00262.
OMAi IMLDIHA.
OrthoDBi EOG7XSTPW.

Enzyme and pathway databases

BioCyci MetaCyc:MONOMER-13461.

Family and domain databases

Gene3Di 3.40.50.720. 1 hit.
InterProi IPR006095. Glu/Leu/Phe/Val_DH.
IPR006096. Glu/Leu/Phe/Val_DH_C.
IPR006097. Glu/Leu/Phe/Val_DH_dimer_dom.
IPR014362. Glu_DH.
IPR016040. NAD(P)-bd_dom.
[Graphical view ]
Pfami PF00208. ELFV_dehydrog. 1 hit.
PF02812. ELFV_dehydrog_N. 1 hit.
[Graphical view ]
PIRSFi PIRSF000185. Glu_DH. 1 hit.
PRINTSi PR00082. GLFDHDRGNASE.
SMARTi SM00839. ELFV_dehydrog. 1 hit.
[Graphical view ]
PROSITEi PS00074. GLFV_DEHYDROGENASE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The complete nucleotide sequence of the Neurospora crassa am (NADP-specific glutamate dehydrogenase) gene."
    Kinnaird J.H., Fincham J.R.S.
    Gene 26:253-260(1983) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "What's in the genome of a filamentous fungus? Analysis of the Neurospora genome sequence."
    Mannhaupt G., Montrone C., Haase D., Mewes H.-W., Aign V., Hoheisel J.D., Fartmann B., Nyakatura G., Kempken F., Maier J., Schulte U.
    Nucleic Acids Res. 31:1944-1954(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987.
  3. "The genome sequence of the filamentous fungus Neurospora crassa."
    Galagan J.E., Calvo S.E., Borkovich K.A., Selker E.U., Read N.D., Jaffe D.B., FitzHugh W., Ma L.-J., Smirnov S., Purcell S., Rehman B., Elkins T., Engels R., Wang S., Nielsen C.B., Butler J., Endrizzi M., Qui D.
    , Ianakiev P., Bell-Pedersen D., Nelson M.A., Werner-Washburne M., Selitrennikoff C.P., Kinsey J.A., Braun E.L., Zelter A., Schulte U., Kothe G.O., Jedd G., Mewes H.-W., Staben C., Marcotte E., Greenberg D., Roy A., Foley K., Naylor J., Stange-Thomann N., Barrett R., Gnerre S., Kamal M., Kamvysselis M., Mauceli E.W., Bielke C., Rudd S., Frishman D., Krystofova S., Rasmussen C., Metzenberg R.L., Perkins D.D., Kroken S., Cogoni C., Macino G., Catcheside D.E.A., Li W., Pratt R.J., Osmani S.A., DeSouza C.P.C., Glass N.L., Orbach M.J., Berglund J.A., Voelker R., Yarden O., Plamann M., Seiler S., Dunlap J.C., Radford A., Aramayo R., Natvig D.O., Alex L.A., Mannhaupt G., Ebbole D.J., Freitag M., Paulsen I., Sachs M.S., Lander E.S., Nusbaum C., Birren B.W.
    Nature 422:859-868(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987.
  4. "The amino acid sequence of Neurospora NADP-specific glutamate dehydrogenase. Peptic and chymotryptic peptides and the complete sequence."
    Holder A.A., Wootton J.C., Baron A.J., Chambers G.K., Fincham J.R.S.
    Biochem. J. 149:757-773(1975) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 2-454.
  5. "Cloning of the am (glutamate dehydrogenase) gene of Neurospora crassa through the use of a synthetic DNA probe."
    Kinnaird J.H., Keighren M.A., Kinsey J.A., Eaton M., Fincham J.R.S.
    Gene 20:387-396(1982) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-23.
  6. "An unstable mutant gene of the am locus of Neurospora results from a small duplication."
    Rambosek J.A., Kinsey J.A.
    Gene 27:101-107(1984) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-25.

Entry informationi

Entry nameiDHE4_NEUCR
AccessioniPrimary (citable) accession number: P00369
Secondary accession number(s): Q7RVE1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: January 23, 2007
Last modified: May 14, 2014
This is version 114 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi