Reviewed, UniProtKB/Swiss-Prot P00369 (DHE4_NEUCR)

Names and origin

Protein names

Recommended name:
    NADP-specific glutamate dehydrogenase
      Short name=NADP-GDH
    EC=1.4.1.4
Alternative name(s):
    NADP-dependent glutamate dehydrogenase

Gene names

Name:	gdh
Synonyms:	am
ORF Names:	18F11.230, NCU01195

Organism

Neurospora crassa [Complete proteome]

Taxonomic identifier

5141 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Pezizomycotina › Sordariomycetes › Sordariomycetidae › Sordariales › Sordariaceae › Neurospora

Protein attributes

Sequence length	454 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Catalytic activity	L-glutamate + H(2)O + NADP(+) = 2-oxoglutarate + NH(3) + NADPH.
Subunit structure	Homohexamer.
Sequence similarities	Belongs to the Glu/Leu/Phe/Val dehydrogenases family.

Ontologies

Keywords
Ligand	NADP
Molecular function	Oxidoreductase
PTM	Acetylation
Technical term	Complete proteome Direct protein sequencing
Gene Ontology (GO)
Biological process	amino acid metabolic process Inferred from electronic annotation. Source: InterPro oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	binding Inferred from electronic annotation. Source: InterPro glutamate dehydrogenase (NADP+) activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed
	Chain	2 – 454	453	NADP-specific glutamate dehydrogenase		PRO_0000182790
Sites
	Active site	114	1	By similarity
Amino acid modifications
	Modified residue	2	1	N-acetylserine
Experimental info
	Sequence conflict	57	1	N → D AA sequence Ref.4
	Sequence conflict	314 – 315	2	QN → ED AA sequence Ref.4
	Sequence conflict	451	1	Missing AA sequence Ref.4

Sequences

	Sequence		Length	Mass (Da)
	P00369-1 [UniParc]. Last modified January 23, 2007. Version 3. Checksum: 908B56A63837DA28	FASTA	454	48,853
	10 20 30 40 50 60 MSNLPSEPEF EQAYKELAYT LENSSLFQKH PEYRTALTVA SIPERVIQFR VVWEDDNGNV 70 80 90 100 110 120 QVNRGYRVQF NSALGPYKGG LRLHPSVNLS ILKFLGFEQI FKNALTGLSM GGGKGGADFD 130 140 150 160 170 180 PKGKSDAEIR RFCCAFMAEL HKHIGADTDV PAGDIGVGGR EIGYMFGAYR KAANRFEGVL 190 200 210 220 230 240 TGKGLSWGGS LIRPEATGYG LVYYVGHMLE YSGAGSYAGK RVALSGSGNV AQYAALKLIE 250 260 270 280 290 300 LGATVVSLSD SKGALVATGE SGITVEDINA VMAIKEARQS LTSFQHAGHL KWIEGARPWL 310 320 330 340 350 360 HVGKVDIALP CATQNEVSKE EAEGLLAAGC KFVAEGSNMG CTLEAIEVFE NNRKEKKGEA 370 380 390 400 410 420 VWYAPGKAAN CGGVAVSGLE MAQNSQRLNW TQAEVDEKLK DIMKNAFFNG LNTAKTYVEA 430 440 450 AEGELPSLVA GSNIAGFVKV AQAMHDQGDW WSKN

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The complete nucleotide sequence of the Neurospora crassa am (NADP-specific glutamate dehydrogenase) gene." Kinnaird J.H., Fincham J.R.S. Gene 26:253-260(1983) [PubMed: 6231215] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"What's in the genome of a filamentous fungus? Analysis of the Neurospora genome sequence." Mannhaupt G., Montrone C., Haase D., Mewes H.-W., Aign V., Hoheisel J.D., Fartmann B., Nyakatura G., Kempken F., Maier J., Schulte U. Nucleic Acids Res. 31:1944-1954(2003) [PubMed: 12655011] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987.
[3]	"The genome sequence of the filamentous fungus Neurospora crassa." Galagan J.E., Calvo S.E., Borkovich K.A., Selker E.U., Read N.D., Jaffe D.B., FitzHugh W., Ma L.-J., Smirnov S., Purcell S., Rehman B., Elkins T., Engels R., Wang S., Nielsen C.B., Butler J., Endrizzi M., Qui D. , Ianakiev P., Bell-Pedersen D., Nelson M.A., Werner-Washburne M., Selitrennikoff C.P., Kinsey J.A., Braun E.L., Zelter A., Schulte U., Kothe G.O., Jedd G., Mewes H.-W., Staben C., Marcotte E., Greenberg D., Roy A., Foley K., Naylor J., Stange-Thomann N., Barrett R., Gnerre S., Kamal M., Kamvysselis M., Mauceli E.W., Bielke C., Rudd S., Frishman D., Krystofova S., Rasmussen C., Metzenberg R.L., Perkins D.D., Kroken S., Cogoni C., Macino G., Catcheside D.E.A., Li W., Pratt R.J., Osmani S.A., DeSouza C.P.C., Glass N.L., Orbach M.J., Berglund J.A., Voelker R., Yarden O., Plamann M., Seiler S., Dunlap J.C., Radford A., Aramayo R., Natvig D.O., Alex L.A., Mannhaupt G., Ebbole D.J., Freitag M., Paulsen I., Sachs M.S., Lander E.S., Nusbaum C., Birren B.W. Nature 422:859-868(2003) [PubMed: 12712197] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987.
[4]	"The amino acid sequence of Neurospora NADP-specific glutamate dehydrogenase. Peptic and chymotryptic peptides and the complete sequence." Holder A.A., Wootton J.C., Baron A.J., Chambers G.K., Fincham J.R.S. Biochem. J. 149:757-773(1975) [PubMed: 1002] [Abstract] Cited for: PROTEIN SEQUENCE OF 2-454.
[5]	"Cloning of the am (glutamate dehydrogenase) gene of Neurospora crassa through the use of a synthetic DNA probe." Kinnaird J.H., Keighren M.A., Kinsey J.A., Eaton M., Fincham J.R.S. Gene 20:387-396(1982) [PubMed: 6299898] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-23.
[6]	"An unstable mutant gene of the am locus of Neurospora results from a small duplication." Rambosek J.A., Kinsey J.A. Gene 27:101-107(1984) [PubMed: 6325296] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-25.

Cross-references

Sequence databases
EMBLGenBankDDBJ	K01409 Genomic DNA. Translation: AAA33558.1. AL670011 Genomic DNA. Translation: CAD21426.1. AABX02000015 Genomic DNA. Translation: EAA32325.1. K01653 Genomic DNA. Translation: AAA33557.1. K02468 Genomic DNA. Translation: AAA33559.1.
PIR	DENCEN. A91506.
RefSeq	XP_961561.1.
3D structure databases
HSSP	HSSP built from PDB template 1AUP based on UniProtKB P24295.
ModBase	Search...
Genome annotation databases
GeneID	3877684.
KEGG	ncr:NCU01195.
NMPDR	fig|5141.1.peg.4621.
Enzyme and pathway databases
BioCyc	MetaCyc:MON-13461.
Family and domain databases
InterPro	IPR006095. Glu/Leu/Phe/Val_DHase. IPR006096. Glu/Leu/Phe/Val_DHase_C. IPR006097. Glu/Leu/Phe/Val_DHase_dimer. IPR014362. Glu_DHase. IPR016040. NAD(P)-bd. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
PANTHER	PTHR11606:SF2. GLFV_DH. 1 hit.
Pfam	PF00208. ELFV_dehydrog. 1 hit. PF02812. ELFV_dehydrog_N. 1 hit. [Graphical view]
PIRSF	PIRSF000185. Glu_DH. 1 hit.
PRINTS	PR00082. GLFDHDRGNASE.
PROSITE	PS00074. GLFV_DEHYDROGENASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DHE4_NEUCR

Accession

Primary (citable) accession number: P00369
Secondary accession number(s): Q7RVE1

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 21, 1986
Last sequence update:	January 23, 2007
Last modified:	November 25, 2008
This is version 78 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)