Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

NADP-specific glutamate dehydrogenase

Gene

gdh

Organism
Neurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

L-glutamate + H2O + NADP+ = 2-oxoglutarate + NH3 + NADPH.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei114PROSITE-ProRule annotation1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Ligandi

NADP

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-13461.

Names & Taxonomyi

Protein namesi
Recommended name:
NADP-specific glutamate dehydrogenase (EC:1.4.1.4)
Short name:
NADP-GDH
Alternative name(s):
NADP-dependent glutamate dehydrogenase
Gene namesi
Name:gdh
Synonyms:am
ORF Names:18F11.230, NCU01195
OrganismiNeurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987)
Taxonomic identifieri367110 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaPezizomycotinaSordariomycetesSordariomycetidaeSordarialesSordariaceaeNeurospora
Proteomesi
  • UP000001805 Componenti: Chromosome 2, Linkage Group V

Organism-specific databases

EuPathDBiFungiDB:NCU01195.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved1 Publication
ChainiPRO_00001827902 – 454NADP-specific glutamate dehydrogenaseAdd BLAST453

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylserine1 Publication1

Keywords - PTMi

Acetylation

Proteomic databases

PRIDEiP00369.

Interactioni

Subunit structurei

Homohexamer.

Structurei

3D structure databases

ProteinModelPortaliP00369.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

HOGENOMiHOG000243799.
InParanoidiP00369.
KOiK00262.
OMAiVPWVDDA.
OrthoDBiEOG092C2DRP.

Family and domain databases

CDDicd05313. NAD_bind_2_Glu_DH. 1 hit.
Gene3Di3.40.50.720. 1 hit.
InterProiIPR006095. Glu/Leu/Phe/Val_DH.
IPR033524. Glu/Leu/Phe/Val_DH_AS.
IPR006096. Glu/Leu/Phe/Val_DH_C.
IPR006097. Glu/Leu/Phe/Val_DH_dimer_dom.
IPR014362. Glu_DH.
IPR016040. NAD(P)-bd_dom.
IPR033922. NAD_bind_Glu_DH.
[Graphical view]
PfamiPF00208. ELFV_dehydrog. 1 hit.
PF02812. ELFV_dehydrog_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000185. Glu_DH. 1 hit.
PRINTSiPR00082. GLFDHDRGNASE.
SMARTiSM00839. ELFV_dehydrog. 1 hit.
[Graphical view]
SUPFAMiSSF51735. SSF51735. 1 hit.
PROSITEiPS00074. GLFV_DEHYDROGENASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P00369-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSNLPSEPEF EQAYKELAYT LENSSLFQKH PEYRTALTVA SIPERVIQFR
60 70 80 90 100
VVWEDDNGNV QVNRGYRVQF NSALGPYKGG LRLHPSVNLS ILKFLGFEQI
110 120 130 140 150
FKNALTGLSM GGGKGGADFD PKGKSDAEIR RFCCAFMAEL HKHIGADTDV
160 170 180 190 200
PAGDIGVGGR EIGYMFGAYR KAANRFEGVL TGKGLSWGGS LIRPEATGYG
210 220 230 240 250
LVYYVGHMLE YSGAGSYAGK RVALSGSGNV AQYAALKLIE LGATVVSLSD
260 270 280 290 300
SKGALVATGE SGITVEDINA VMAIKEARQS LTSFQHAGHL KWIEGARPWL
310 320 330 340 350
HVGKVDIALP CATQNEVSKE EAEGLLAAGC KFVAEGSNMG CTLEAIEVFE
360 370 380 390 400
NNRKEKKGEA VWYAPGKAAN CGGVAVSGLE MAQNSQRLNW TQAEVDEKLK
410 420 430 440 450
DIMKNAFFNG LNTAKTYVEA AEGELPSLVA GSNIAGFVKV AQAMHDQGDW

WSKN
Length:454
Mass (Da):48,853
Last modified:January 23, 2007 - v3
Checksum:i908B56A63837DA28
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti57N → D AA sequence (PubMed:1002).Curated1
Sequence conflicti314 – 315QN → ED AA sequence (PubMed:1002).Curated2
Sequence conflicti451Missing AA sequence (PubMed:1002).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
K01409 Genomic DNA. Translation: AAA33558.1.
AL670011 Genomic DNA. Translation: CAD21426.1.
CM002240 Genomic DNA. Translation: EAA32325.1.
K01653 Genomic DNA. Translation: AAA33557.1.
K02468 Genomic DNA. Translation: AAA33559.1.
PIRiA91506. DENCEN.
RefSeqiXP_961561.1. XM_956468.3.

Genome annotation databases

EnsemblFungiiEAA32325; EAA32325; NCU01195.
GeneIDi3877684.
KEGGincr:NCU01195.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
K01409 Genomic DNA. Translation: AAA33558.1.
AL670011 Genomic DNA. Translation: CAD21426.1.
CM002240 Genomic DNA. Translation: EAA32325.1.
K01653 Genomic DNA. Translation: AAA33557.1.
K02468 Genomic DNA. Translation: AAA33559.1.
PIRiA91506. DENCEN.
RefSeqiXP_961561.1. XM_956468.3.

3D structure databases

ProteinModelPortaliP00369.
ModBaseiSearch...
MobiDBiSearch...

Proteomic databases

PRIDEiP00369.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiEAA32325; EAA32325; NCU01195.
GeneIDi3877684.
KEGGincr:NCU01195.

Organism-specific databases

EuPathDBiFungiDB:NCU01195.

Phylogenomic databases

HOGENOMiHOG000243799.
InParanoidiP00369.
KOiK00262.
OMAiVPWVDDA.
OrthoDBiEOG092C2DRP.

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-13461.

Family and domain databases

CDDicd05313. NAD_bind_2_Glu_DH. 1 hit.
Gene3Di3.40.50.720. 1 hit.
InterProiIPR006095. Glu/Leu/Phe/Val_DH.
IPR033524. Glu/Leu/Phe/Val_DH_AS.
IPR006096. Glu/Leu/Phe/Val_DH_C.
IPR006097. Glu/Leu/Phe/Val_DH_dimer_dom.
IPR014362. Glu_DH.
IPR016040. NAD(P)-bd_dom.
IPR033922. NAD_bind_Glu_DH.
[Graphical view]
PfamiPF00208. ELFV_dehydrog. 1 hit.
PF02812. ELFV_dehydrog_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000185. Glu_DH. 1 hit.
PRINTSiPR00082. GLFDHDRGNASE.
SMARTiSM00839. ELFV_dehydrog. 1 hit.
[Graphical view]
SUPFAMiSSF51735. SSF51735. 1 hit.
PROSITEiPS00074. GLFV_DEHYDROGENASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiDHE4_NEUCR
AccessioniPrimary (citable) accession number: P00369
Secondary accession number(s): Q7RVE1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: January 23, 2007
Last modified: November 30, 2016
This is version 131 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.