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P00368

- DHE3_CHICK

UniProt

P00368 - DHE3_CHICK

Protein

Glutamate dehydrogenase 1, mitochondrial

Gene

GLUD1

Organism
Gallus gallus (Chicken)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 98 (01 Oct 2014)
      Sequence version 1 (21 Jul 1986)
      Previous versions | rss
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    Functioni

    Mitochondrial glutamate dehydrogenase that converts L-glutamate into alpha-ketoglutarate. Plays a key role in glutamine anaplerosis by producing alpha-ketoglutarate, an important intermediate in the tricarboxylic acid cycle By similarity.By similarity

    Catalytic activityi

    L-glutamate + H2O + NAD(P)+ = 2-oxoglutarate + NH3 + NAD(P)H.PROSITE-ProRule annotation

    Enzyme regulationi

    Subject to allosteric regulation. Activated by ADP. Inhibited by GTP and ATP By similarity.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei93 – 931SubstrateBy similarity
    Binding sitei117 – 1171SubstrateBy similarity
    Binding sitei122 – 1221NADBy similarity
    Active sitei129 – 12911 PublicationPROSITE-ProRule annotation
    Binding sitei198 – 1981NADBy similarity
    Binding sitei212 – 2121GTPBy similarity
    Binding sitei216 – 2161GTPBy similarity
    Binding sitei265 – 2651GTPBy similarity
    Binding sitei268 – 2681GTPBy similarity
    Binding sitei384 – 3841SubstrateBy similarity
    Binding sitei389 – 3891NADBy similarity
    Binding sitei395 – 3951ADPBy similarity
    Binding sitei461 – 4611ADPBy similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi87 – 893NADBy similarity

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. glutamate dehydrogenase [NAD(P)+] activity Source: UniProtKB
    3. GTP binding Source: UniProtKB-KW

    GO - Biological processi

    1. glutamine metabolic process Source: UniProtKB
    2. tricarboxylic acid metabolic process Source: UniProtKB

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Ligandi

    ATP-binding, GTP-binding, NADP, Nucleotide-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Glutamate dehydrogenase 1, mitochondrial (EC:1.4.1.3)
    Short name:
    GDH 1
    Gene namesi
    Name:GLUD1
    Synonyms:GLUD
    OrganismiGallus gallus (Chicken)
    Taxonomic identifieri9031 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiTestudines + Archosauria groupArchosauriaDinosauriaSaurischiaTheropodaCoelurosauriaAvesNeognathaeGalliformesPhasianidaePhasianinaeGallus
    ProteomesiUP000000539: Unplaced

    Subcellular locationi

    GO - Cellular componenti

    1. mitochondrial matrix Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Mitochondrion

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 503503Glutamate dehydrogenase 1, mitochondrialPRO_0000182742Add
    BLAST

    Proteomic databases

    PaxDbiP00368.
    PRIDEiP00368.

    Interactioni

    Subunit structurei

    Homohexamer.

    Protein-protein interaction databases

    STRINGi9031.ENSGALP00000003139.

    Structurei

    3D structure databases

    ProteinModelPortaliP00368.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiCOG0334.
    HOGENOMiHOG000243801.
    HOVERGENiHBG005479.
    InParanoidiP00368.
    PhylomeDBiP00368.

    Family and domain databases

    Gene3Di3.40.50.720. 1 hit.
    InterProiIPR006095. Glu/Leu/Phe/Val_DH.
    IPR006096. Glu/Leu/Phe/Val_DH_C.
    IPR006097. Glu/Leu/Phe/Val_DH_dimer_dom.
    IPR014362. Glu_DH.
    IPR016040. NAD(P)-bd_dom.
    [Graphical view]
    PfamiPF00208. ELFV_dehydrog. 1 hit.
    PF02812. ELFV_dehydrog_N. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000185. Glu_DH. 1 hit.
    PRINTSiPR00082. GLFDHDRGNASE.
    SMARTiSM00839. ELFV_dehydrog. 1 hit.
    [Graphical view]
    PROSITEiPS00074. GLFV_DEHYDROGENASE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P00368-1 [UniParc]FASTAAdd to Basket

    « Hide

    CZAADKEDDP NFFKMVEGFF DRGASIVEDK LVEGLRTRQS MEQRRHRVRG    50
    ILRIIKPCNH VLSVSFPIKR DDGZWEVIEG YRAQHSHQRT PCKGGIRYSL 100
    DVSVDEVKAL ASLMTYKCAV VDVPFGGAKA GVKINPKNYT DEDLEKITRR 150
    FTMELAKKGF IGPGVDVPAP NMSTGEREMS WIADTYASTI GHYDINAHAC 200
    VTKPGISQGG IHGRISATGR GLFGHIENFI ENASYMSILG MTPGFGDKTF 250
    AVQGFGNVGL HSMRYLHRFG AKCVAVGEFD GSIWNPDGID PKELEDYKLQ 300
    HGTIMGFPKA QKLEGSILET DCDILIPAAS EKQLTKANAH KVKAKIIAEG 350
    ANGPTTPQAD KIFLERNIMV IPDLYLNAGG VTVSAFZZKN LNHVSYGRLT 400
    FKYERDSNYH LLMSVQESLE RKFGKHGGTI PVVPTAEFQD RISGASEKDI 450
    VHSGLAYTME RSARQIMRTA MKYNLGLDLR TAAYVNAIEK VFKVYNEAGL 500
    TFT 503
    Length:503
    Mass (Da):55,712
    Last modified:July 21, 1986 - v1
    Checksum:i340DDB45BB0A5B04
    GO

    Sequence databases

    PIRiA00380. DECHE.
    UniGeneiGga.16450.

    Cross-referencesi

    Sequence databases

    PIRi A00380. DECHE.
    UniGenei Gga.16450.

    3D structure databases

    ProteinModelPortali P00368.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 9031.ENSGALP00000003139.

    Proteomic databases

    PaxDbi P00368.
    PRIDEi P00368.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Phylogenomic databases

    eggNOGi COG0334.
    HOGENOMi HOG000243801.
    HOVERGENi HBG005479.
    InParanoidi P00368.
    PhylomeDBi P00368.

    Miscellaneous databases

    PROi P00368.

    Family and domain databases

    Gene3Di 3.40.50.720. 1 hit.
    InterProi IPR006095. Glu/Leu/Phe/Val_DH.
    IPR006096. Glu/Leu/Phe/Val_DH_C.
    IPR006097. Glu/Leu/Phe/Val_DH_dimer_dom.
    IPR014362. Glu_DH.
    IPR016040. NAD(P)-bd_dom.
    [Graphical view ]
    Pfami PF00208. ELFV_dehydrog. 1 hit.
    PF02812. ELFV_dehydrog_N. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF000185. Glu_DH. 1 hit.
    PRINTSi PR00082. GLFDHDRGNASE.
    SMARTi SM00839. ELFV_dehydrog. 1 hit.
    [Graphical view ]
    PROSITEi PS00074. GLFV_DEHYDROGENASE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Amino acd sequence of chicken liver glutamate dehydrogenase."
      Moon K., Piszkiewicz D., Smith E.L.
      J. Biol. Chem. 248:3093-3107(1973) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE, ACTIVE SITE.
      Tissue: Liver.

    Entry informationi

    Entry nameiDHE3_CHICK
    AccessioniPrimary (citable) accession number: P00368
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 21, 1986
    Last sequence update: July 21, 1986
    Last modified: October 1, 2014
    This is version 98 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    ADP can occupy the NADH binding site and activate the enzyme.By similarity

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3