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P00368

- DHE3_CHICK

UniProt

P00368 - DHE3_CHICK

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Protein
Glutamate dehydrogenase 1, mitochondrial
Gene
GLUD1, GLUD
Organism
Gallus gallus (Chicken)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at protein leveli

Functioni

Mitochondrial glutamate dehydrogenase that converts L-glutamate into alpha-ketoglutarate. Plays a key role in glutamine anaplerosis by producing alpha-ketoglutarate, an important intermediate in the tricarboxylic acid cycle By similarity.

Catalytic activityi

L-glutamate + H2O + NAD(P)+ = 2-oxoglutarate + NH3 + NAD(P)H.

Enzyme regulationi

Subject to allosteric regulation. Activated by ADP. Inhibited by GTP and ATP By similarity.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei93 – 931Substrate By similarity
Binding sitei117 – 1171Substrate By similarity
Binding sitei122 – 1221NAD By similarity
Active sitei129 – 12911 Publication
Binding sitei198 – 1981NAD By similarity
Binding sitei212 – 2121GTP By similarity
Binding sitei216 – 2161GTP By similarity
Binding sitei265 – 2651GTP By similarity
Binding sitei268 – 2681GTP By similarity
Binding sitei384 – 3841Substrate By similarity
Binding sitei389 – 3891NAD By similarity
Binding sitei395 – 3951ADP By similarity
Binding sitei461 – 4611ADP By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi87 – 893NAD By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. GTP binding Source: UniProtKB-KW
  3. glutamate dehydrogenase [NAD(P)+] activity Source: UniProtKB
Complete GO annotation...

GO - Biological processi

  1. glutamine metabolic process Source: UniProtKB
  2. tricarboxylic acid metabolic process Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Ligandi

ATP-binding, GTP-binding, NADP, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Glutamate dehydrogenase 1, mitochondrial (EC:1.4.1.3)
Short name:
GDH 1
Gene namesi
Name:GLUD1
Synonyms:GLUD
OrganismiGallus gallus (Chicken)
Taxonomic identifieri9031 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiTestudines + Archosauria groupArchosauriaDinosauriaSaurischiaTheropodaCoelurosauriaAvesNeognathaeGalliformesPhasianidaePhasianinaeGallus
ProteomesiUP000000539: Unplaced

Subcellular locationi

GO - Cellular componenti

  1. mitochondrial matrix Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 503503Glutamate dehydrogenase 1, mitochondrial
PRO_0000182742Add
BLAST

Proteomic databases

PaxDbiP00368.
PRIDEiP00368.

Interactioni

Subunit structurei

Homohexamer.

Protein-protein interaction databases

STRINGi9031.ENSGALP00000003139.

Structurei

3D structure databases

ProteinModelPortaliP00368.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0334.
HOGENOMiHOG000243801.
HOVERGENiHBG005479.
InParanoidiP00368.
PhylomeDBiP00368.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
InterProiIPR006095. Glu/Leu/Phe/Val_DH.
IPR006096. Glu/Leu/Phe/Val_DH_C.
IPR006097. Glu/Leu/Phe/Val_DH_dimer_dom.
IPR014362. Glu_DH.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PfamiPF00208. ELFV_dehydrog. 1 hit.
PF02812. ELFV_dehydrog_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000185. Glu_DH. 1 hit.
PRINTSiPR00082. GLFDHDRGNASE.
SMARTiSM00839. ELFV_dehydrog. 1 hit.
[Graphical view]
PROSITEiPS00074. GLFV_DEHYDROGENASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P00368-1 [UniParc]FASTAAdd to Basket

« Hide

CZAADKEDDP NFFKMVEGFF DRGASIVEDK LVEGLRTRQS MEQRRHRVRG    50
ILRIIKPCNH VLSVSFPIKR DDGZWEVIEG YRAQHSHQRT PCKGGIRYSL 100
DVSVDEVKAL ASLMTYKCAV VDVPFGGAKA GVKINPKNYT DEDLEKITRR 150
FTMELAKKGF IGPGVDVPAP NMSTGEREMS WIADTYASTI GHYDINAHAC 200
VTKPGISQGG IHGRISATGR GLFGHIENFI ENASYMSILG MTPGFGDKTF 250
AVQGFGNVGL HSMRYLHRFG AKCVAVGEFD GSIWNPDGID PKELEDYKLQ 300
HGTIMGFPKA QKLEGSILET DCDILIPAAS EKQLTKANAH KVKAKIIAEG 350
ANGPTTPQAD KIFLERNIMV IPDLYLNAGG VTVSAFZZKN LNHVSYGRLT 400
FKYERDSNYH LLMSVQESLE RKFGKHGGTI PVVPTAEFQD RISGASEKDI 450
VHSGLAYTME RSARQIMRTA MKYNLGLDLR TAAYVNAIEK VFKVYNEAGL 500
TFT 503
Length:503
Mass (Da):55,712
Last modified:July 21, 1986 - v1
Checksum:i340DDB45BB0A5B04
GO

Sequence databases

PIRiA00380. DECHE.
UniGeneiGga.16450.

Cross-referencesi

Sequence databases

PIRi A00380. DECHE.
UniGenei Gga.16450.

3D structure databases

ProteinModelPortali P00368.
ModBasei Search...

Protein-protein interaction databases

STRINGi 9031.ENSGALP00000003139.

Proteomic databases

PaxDbi P00368.
PRIDEi P00368.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Phylogenomic databases

eggNOGi COG0334.
HOGENOMi HOG000243801.
HOVERGENi HBG005479.
InParanoidi P00368.
PhylomeDBi P00368.

Miscellaneous databases

PROi P00368.

Family and domain databases

Gene3Di 3.40.50.720. 1 hit.
InterProi IPR006095. Glu/Leu/Phe/Val_DH.
IPR006096. Glu/Leu/Phe/Val_DH_C.
IPR006097. Glu/Leu/Phe/Val_DH_dimer_dom.
IPR014362. Glu_DH.
IPR016040. NAD(P)-bd_dom.
[Graphical view ]
Pfami PF00208. ELFV_dehydrog. 1 hit.
PF02812. ELFV_dehydrog_N. 1 hit.
[Graphical view ]
PIRSFi PIRSF000185. Glu_DH. 1 hit.
PRINTSi PR00082. GLFDHDRGNASE.
SMARTi SM00839. ELFV_dehydrog. 1 hit.
[Graphical view ]
PROSITEi PS00074. GLFV_DEHYDROGENASE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Amino acd sequence of chicken liver glutamate dehydrogenase."
    Moon K., Piszkiewicz D., Smith E.L.
    J. Biol. Chem. 248:3093-3107(1973) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE, ACTIVE SITE.
    Tissue: Liver.

Entry informationi

Entry nameiDHE3_CHICK
AccessioniPrimary (citable) accession number: P00368
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: July 21, 1986
Last modified: April 16, 2014
This is version 97 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Miscellaneous

ADP can occupy the NADH binding site and activate the enzyme By similarity.

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi