NAD-specific glutamate dehydrogenase

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Reviewed, UniProtKB/Swiss-Prot P00365 (DHE2_NEUCR)

Last modified January 19, 2010. Version 76. History...

Clusters with 100%, 90%, 50% identity |

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Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein names

Recommended name:
    NAD-specific glutamate dehydrogenase
      Short name=NAD-GDH
    EC=1.4.1.2

Gene names

Name:	gdh-1
ORF Names:	NCU00461

Organism

Neurospora crassa [Complete proteome]

Taxonomic identifier

5141 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Pezizomycotina › Sordariomycetes › Sordariomycetidae › Sordariales › Sordariaceae › Neurospora

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Protein attributes

Sequence length	1050 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level.

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General annotation (Comments)

Catalytic activity	L-glutamate + H₂O + NAD⁺ = 2-oxoglutarate + NH₃ + NADH.
Subunit structure	Homotetramer.
Sequence similarities	Belongs to the Glu/Leu/Phe/Val dehydrogenases family.

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Ontologies

Keywords
Ligand	NAD
Molecular function	Oxidoreductase
Technical term	Complete proteome Direct protein sequencing
Gene Ontology (GO)
Biological process	glutamate catabolic process to 2-oxoglutarate Inferred from electronic annotation. Source: InterPro oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	binding Inferred from electronic annotation. Source: InterPro glutamate dehydrogenase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

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Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 1050

1050

NAD-specific glutamate dehydrogenase

PRO_0000182732

Sites

Active site

594

By similarity

Experimental info

Sequence conflict

3 – 5

SPS → APD AA sequence Ref.3

Sequence conflict

H → R AA sequence Ref.3

Sequence conflict

31 – 41

HVSYPKVNGNG → VPYSKVDGGN AA sequence Ref.3

Sequence conflict

V → Q AA sequence Ref.3

Sequence conflict

113 – 114

TS → ST AA sequence Ref.3

Sequence conflict

153

T → TTN AA sequence Ref.3

Sequence conflict

154

Missing in AAB28355. Ref.1

Sequence conflict

216

P → PEGDP AA sequence Ref.3

Sequence conflict

345 – 347

LPQ → PQL AA sequence Ref.3

Sequence conflict

351 – 352

HN → NH AA sequence Ref.3

Sequence conflict

461 – 464

EVLS → SEVL AA sequence Ref.4

Sequence conflict

637

L → V in AAB28355. Ref.1

Sequence conflict

660

T → I in AAB28355. Ref.1

Sequence conflict

708 – 709

Missing in AAB28355. Ref.1

Sequence conflict

753

N → D AA sequence Ref.4

Sequence conflict

788

D → N in AAA33601. Ref.5

Sequence conflict

799 – 801

VVH → HV AA sequence Ref.4

Sequence conflict

845 – 846

ST → TS AA sequence Ref.4

Sequence conflict

909

D → N AA sequence Ref.4

Sequence conflict

1024

V → VE AA sequence Ref.4

Sequence conflict

1035

Missing AA sequence Ref.4

Sequence conflict

1038 – 1039

DF → AD AA sequence Ref.4

Sequence conflict

1041

S → T in AAB28355. Ref.1

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Sequences

Sequence

Length

Mass (Da)

Tools

P00365-1 [UniParc].

Last modified June 7, 2005. Version 4.
Checksum: CDCACCFD221D2CB3
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FASTA

1,050

118,584

        10         20         30         40         50         60 
MDSPSAPVPA HKLVDRLKDQ TPRHPSPQPT HVSYPKVNGN GHRVLRSATV GYVAPVFQGK 

        70         80         90        100        110        120 
AEQMKQVKNI IVQGGWIPET LVDGQIAWFY NELGIDDVYF QLENPQAVAN HITSLYAAKV 

       130        140        150        160        170        180 
AAFSREDKRE EIRLDMEASD HAIYIDTSEP GMTSFDGPRY EHRLESKYLD GDDTSKRFRV 

       190        200        210        220        230        240 
ETFRSPGVLG QKENSKAALR CYFVYQCLFV DSNADPKETR LEVISDRMFL AKATKNTKQI 

       250        260        270        280        290        300 
YQDIIQVAVS RHGPVIEVFD IEGSEEMRLV VAFRSRTAKG IFSALSDLYH YYGVTSSRKY 

       310        320        330        340        350        360 
VEQFSNGITV MSIYLRPAAN IDGKHPPLEQ SIHQITKEIS LLYCLPQNKF HNMFASGELS 

       370        380        390        400        410        420 
LQETIYAHCV WVFVQHFLNR LGTEYTSLIA ALDPKNNSHV EILSKMKKRL RTETFTPDYI 

       430        440        450        460        470        480 
LEIISSHPQL VRALYASFAS VHLRVGSDYD RHLIAPTPVM EVLSDARLKE KITKDVSNEH 

       490        500        510        520        530        540 
EEMVMTAFRV FNNAVLKTNF FTPTKVALSF RLNPSFLPEV EYPKPLYGMF LVITSESRGF 

       550        560        570        580        590        600 
HLRFKDIARG GIRIVKSRSK EAYQINARNL FDENYGLAST QQRKNKDIPE GGSKGVILLD 

       610        620        630        640        650        660 
PKQQDRHREA FEKYIDSILD LLLKAETPGI KNPIVDLYGK EEILFMGPDE NTADLVDWAT 

       670        680        690        700        710        720 
EHARARGAPW WKSFFTGKSP RLGGIPHDSY GMTTLSVREY VKGIYRKLEL DPSKIRKMQT 

       730        740        750        760        770        780 
GGPDGDLGSN EILLSNETYT AIVDGSGVLC DPNGIDKDEL RRLAKARAMI SNFDIAKLSK 

       790        800        810        820        830        840 
DGYRVLCDDT NVTLPNGEVV HNGTAFRNTY HLRDNGITDM FVPCGGRPES IDLSSVNKLI 

       850        860        870        880        890        900 
KDGKSTIPYI VEGANLFITQ DAKLRLEEAG CIVYKDASAN KGGVTSSSLE VLASLSFDDK 

       910        920        930        940        950        960 
GFVTHMCHDS RGNAPEFYQA YVKEVQNKIQ DNARLEFEAI WREHEQTGLP RSVLSDKLSL 

       970        980        990       1000       1010       1020 
AITSLDEDLQ RSELWDNEKI RRSVLADALP NLLINKIGLD TIIERVPDSY LRAIFGSYLA 

      1030       1040       1050 
SRFVYEFGSS PSQFAFYDFM SKRMGNINKE

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References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"NAD(+)-specific glutamate dehydrogenase of Neurospora crassa: cloning, complete nucleotide sequence, and gene mapping." Kapoor M., Vijayaraghavan Y., Kadonaga R., LaRue K.E. Biochem. Cell Biol. 71:205-219(1993) [PubMed: 8398079] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"The genome sequence of the filamentous fungus Neurospora crassa." Galagan J.E., Calvo S.E., Borkovich K.A., Selker E.U., Read N.D., Jaffe D.B., FitzHugh W., Ma L.-J., Smirnov S., Purcell S., Rehman B., Elkins T., Engels R., Wang S., Nielsen C.B., Butler J., Endrizzi M., Qui D. Birren B.W. Nature 422:859-868(2003) [PubMed: 12712197] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987.
[3]	"Nicotinamide adenine dinucleotide-specific glutamate dehydrogenase of Neurospora crassa. Isolation and sequences of several cyanogen bromide peptides from the NH2-terminal portion of the peptide chain." Haberland M.E., Smith E.L. J. Biol. Chem. 255:7984-7992(1980) [PubMed: 6447150] [Abstract] Cited for: PROTEIN SEQUENCE OF 1-44 AND 47-353.
[4]	"Nicotinamide adenine dinucleotide-specific glutamate dehydrogenase of Neurospora. IV. The COOH-terminal 669 residues of the peptide chain; comparison with other glutamate dehydrogenases." Austen B.M., Haberland M.E., Nyc J.F., Smith E.L. J. Biol. Chem. 252:8142-8149(1977) [PubMed: 21191] [Abstract] Cited for: PROTEIN SEQUENCE OF 381-1050.
[5]	"NAD-specific glutamate dehydrogenase of Neurospora crassa. cDNA cloning and gene expression during derepression." Vierula P.J., Kapoor M. J. Biol. Chem. 264:1108-1114(1989) [PubMed: 2521336] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 642-948.

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Cross-references

Sequence databases
EMBL GenBank DDBJ	S66039 Genomic DNA. Translation: AAB28355.1. AABX02000001 Genomic DNA. Translation: EAA27544.1. M23436 mRNA. Translation: AAA33601.1.
PIR	DENCED. A92284. T46599.
RefSeq	XP_956780.1.
3D structure databases
SMR	P00365. Positions 479-975.
ModBase	Search...
Protein-protein interaction databases
STRING	P00365.
Genome annotation databases
GeneID	3872927.
KEGG	ncr:NCU00461.
NMPDR	fig\|5141.1.peg.480.
Phylogenomic databases
eggNOG	fuNOG04119.
OrthoDB	EOG925798.
PhylomeDB	P00365.
Enzyme and pathway databases
BRENDA	1.4.1.2. 266.
Family and domain databases
InterPro	IPR006095. Glu/Leu/Phe/Val_DH. IPR006096. Glu/Leu/Phe/Val_DH_C. IPR016210. Glu_DH_NAD-dep. IPR016040. NAD(P)-bd_dom. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
Pfam	PF00208. ELFV_dehydrog. 1 hit. [Graphical view]
PIRSF	PIRSF000184. GDH_NAD. 1 hit.
SMART	SM00839. ELFV_dehydrog. 1 hit. [Graphical view]
PROSITE	PS00074. GLFV_DEHYDROGENASE. 1 hit. [Graphical view]
ProtoNet	Search...

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Entry information

Entry name

DHE2_NEUCR

Accession

Primary (citable) accession number: P00365
Secondary accession number(s): Q02222, Q7RUZ7

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 21, 1986
Last sequence update:	June 7, 2005
Last modified:	January 19, 2010
This is version 76 of the entry and version 4 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)

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Relevant documents

SIMILARITY comments

Index of protein domains and families

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Sites

Experimental info

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Genome annotation databases

Phylogenomic databases

Enzyme and pathway databases

Family and domain databases

Entry information

Relevant documents