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P00360

- G3P1_YEAST

UniProt

P00360 - G3P1_YEAST

Protein

Glyceraldehyde-3-phosphate dehydrogenase 1

Gene

TDH1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 157 (01 Oct 2014)
      Sequence version 3 (23 Jan 2007)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    D-glyceraldehyde 3-phosphate + phosphate + NAD+ = 3-phospho-D-glyceroyl phosphate + NADH.PROSITE-ProRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei33 – 331NADBy similarity
    Binding sitei78 – 781NAD; via carbonyl oxygenBy similarity
    Active sitei150 – 1501NucleophilePROSITE-ProRule annotation
    Sitei177 – 1771Activates thiol group during catalysisBy similarity
    Binding sitei180 – 1801Glyceraldehyde 3-phosphateBy similarity
    Binding sitei232 – 2321Glyceraldehyde 3-phosphateBy similarity
    Binding sitei314 – 3141NADBy similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi11 – 122NADBy similarity

    GO - Molecular functioni

    1. glyceraldehyde-3-phosphate dehydrogenase (NAD+) (phosphorylating) activity Source: SGD
    2. NAD binding Source: InterPro
    3. NADP binding Source: InterPro

    GO - Biological processi

    1. gluconeogenesis Source: SGD
    2. glycolytic process Source: SGD

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Biological processi

    Glycolysis

    Keywords - Ligandi

    NAD

    Enzyme and pathway databases

    BioCyciYEAST:YJL052W-MONOMER.
    SABIO-RKP00360.
    UniPathwayiUPA00109; UER00184.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Glyceraldehyde-3-phosphate dehydrogenase 1 (EC:1.2.1.12)
    Short name:
    GAPDH 1
    Gene namesi
    Name:TDH1
    Synonyms:GPD1, SSS2
    Ordered Locus Names:YJL052W
    ORF Names:J1154
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome X

    Organism-specific databases

    SGDiS000003588. TDH1.

    Subcellular locationi

    Cytoplasm 1 Publication

    GO - Cellular componenti

    1. cytoplasm Source: SGD
    2. fungal-type cell wall Source: SGD
    3. lipid particle Source: SGD
    4. mitochondrion Source: SGD

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 332332Glyceraldehyde-3-phosphate dehydrogenase 1PRO_0000145589Add
    BLAST

    Proteomic databases

    MaxQBiP00360.
    PaxDbiP00360.
    PeptideAtlasiP00360.
    PRIDEiP00360.

    2D gel databases

    SWISS-2DPAGEP00360.

    Expressioni

    Gene expression databases

    GenevestigatoriP00360.

    Interactioni

    Subunit structurei

    Homotetramer.

    Protein-protein interaction databases

    BioGridi33703. 59 interactions.
    DIPiDIP-4304N.
    IntActiP00360. 23 interactions.
    MINTiMINT-491516.
    STRINGi4932.YJL052W.

    Structurei

    3D structure databases

    ProteinModelPortaliP00360.
    SMRiP00360. Positions 1-332.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni149 – 1513Glyceraldehyde 3-phosphate bindingBy similarity
    Regioni209 – 2102Glyceraldehyde 3-phosphate bindingBy similarity

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiCOG0057.
    GeneTreeiENSGT00720000108591.
    HOGENOMiHOG000071678.
    KOiK00134.
    OMAiALRVVIC.
    OrthoDBiEOG70W3Q6.

    Family and domain databases

    Gene3Di3.40.50.720. 1 hit.
    InterProiIPR020831. GlycerAld/Erythrose_P_DH.
    IPR020830. GlycerAld_3-P_DH_AS.
    IPR020829. GlycerAld_3-P_DH_cat.
    IPR020828. GlycerAld_3-P_DH_NAD(P)-bd.
    IPR006424. Glyceraldehyde-3-P_DH_1.
    IPR016040. NAD(P)-bd_dom.
    [Graphical view]
    PANTHERiPTHR10836. PTHR10836. 1 hit.
    PfamiPF02800. Gp_dh_C. 1 hit.
    PF00044. Gp_dh_N. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000149. GAP_DH. 1 hit.
    PRINTSiPR00078. G3PDHDRGNASE.
    SMARTiSM00846. Gp_dh_N. 1 hit.
    [Graphical view]
    TIGRFAMsiTIGR01534. GAPDH-I. 1 hit.
    PROSITEiPS00071. GAPDH. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P00360-1 [UniParc]FASTAAdd to Basket

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    MIRIAINGFG RIGRLVLRLA LQRKDIEVVA VNDPFISNDY AAYMVKYDST    50
    HGRYKGTVSH DDKHIIIDGV KIATYQERDP ANLPWGSLKI DVAVDSTGVF 100
    KELDTAQKHI DAGAKKVVIT APSSSAPMFV VGVNHTKYTP DKKIVSNASC 150
    TTNCLAPLAK VINDAFGIEE GLMTTVHSMT ATQKTVDGPS HKDWRGGRTA 200
    SGNIIPSSTG AAKAVGKVLP ELQGKLTGMA FRVPTVDVSV VDLTVKLEKE 250
    ATYDQIKKAV KAAAEGPMKG VLGYTEDAVV SSDFLGDTHA SIFDASAGIQ 300
    LSPKFVKLIS WYDNEYGYSA RVVDLIEYVA KA 332
    Length:332
    Mass (Da):35,750
    Last modified:January 23, 2007 - v3
    Checksum:i4C116C86EAF3DB70
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti248 – 2481E → A in CAA24609. (PubMed:6833300)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    V01302 Genomic DNA. Translation: CAA24609.1.
    Z49327 Genomic DNA. Translation: CAA89343.1.
    AY693001 Genomic DNA. Translation: AAT93020.1.
    BK006943 Genomic DNA. Translation: DAA08747.1.
    PIRiS56824. DEBYG3.
    RefSeqiNP_012483.3. NM_001181485.3.

    Genome annotation databases

    EnsemblFungiiYJL052W; YJL052W; YJL052W.
    GeneIDi853395.
    KEGGisce:YJL052W.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    V01302 Genomic DNA. Translation: CAA24609.1 .
    Z49327 Genomic DNA. Translation: CAA89343.1 .
    AY693001 Genomic DNA. Translation: AAT93020.1 .
    BK006943 Genomic DNA. Translation: DAA08747.1 .
    PIRi S56824. DEBYG3.
    RefSeqi NP_012483.3. NM_001181485.3.

    3D structure databases

    ProteinModelPortali P00360.
    SMRi P00360. Positions 1-332.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 33703. 59 interactions.
    DIPi DIP-4304N.
    IntActi P00360. 23 interactions.
    MINTi MINT-491516.
    STRINGi 4932.YJL052W.

    2D gel databases

    SWISS-2DPAGE P00360.

    Proteomic databases

    MaxQBi P00360.
    PaxDbi P00360.
    PeptideAtlasi P00360.
    PRIDEi P00360.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YJL052W ; YJL052W ; YJL052W .
    GeneIDi 853395.
    KEGGi sce:YJL052W.

    Organism-specific databases

    SGDi S000003588. TDH1.

    Phylogenomic databases

    eggNOGi COG0057.
    GeneTreei ENSGT00720000108591.
    HOGENOMi HOG000071678.
    KOi K00134.
    OMAi ALRVVIC.
    OrthoDBi EOG70W3Q6.

    Enzyme and pathway databases

    UniPathwayi UPA00109 ; UER00184 .
    BioCyci YEAST:YJL052W-MONOMER.
    SABIO-RK P00360.

    Miscellaneous databases

    NextBioi 973876.
    PROi P00360.

    Gene expression databases

    Genevestigatori P00360.

    Family and domain databases

    Gene3Di 3.40.50.720. 1 hit.
    InterProi IPR020831. GlycerAld/Erythrose_P_DH.
    IPR020830. GlycerAld_3-P_DH_AS.
    IPR020829. GlycerAld_3-P_DH_cat.
    IPR020828. GlycerAld_3-P_DH_NAD(P)-bd.
    IPR006424. Glyceraldehyde-3-P_DH_1.
    IPR016040. NAD(P)-bd_dom.
    [Graphical view ]
    PANTHERi PTHR10836. PTHR10836. 1 hit.
    Pfami PF02800. Gp_dh_C. 1 hit.
    PF00044. Gp_dh_N. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF000149. GAP_DH. 1 hit.
    PRINTSi PR00078. G3PDHDRGNASE.
    SMARTi SM00846. Gp_dh_N. 1 hit.
    [Graphical view ]
    TIGRFAMsi TIGR01534. GAPDH-I. 1 hit.
    PROSITEi PS00071. GAPDH. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Homologous nucleotide sequences at the 5' termini of messenger RNAs synthesized from the yeast enolase and glyceraldehyde-3-phosphate dehydrogenase gene families. The primary structure of a third yeast glyceraldehyde-3-phosphate dehydrogenase gene."
      Holland J.P., Labieniec L., Swimmer C., Holland M.J.
      J. Biol. Chem. 258:5291-5299(1983) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. "Complete nucleotide sequence of Saccharomyces cerevisiae chromosome X."
      Galibert F., Alexandraki D., Baur A., Boles E., Chalwatzis N., Chuat J.-C., Coster F., Cziepluch C., de Haan M., Domdey H., Durand P., Entian K.-D., Gatius M., Goffeau A., Grivell L.A., Hennemann A., Herbert C.J., Heumann K.
      , Hilger F., Hollenberg C.P., Huang M.-E., Jacq C., Jauniaux J.-C., Katsoulou C., Kirchrath L., Kleine K., Kordes E., Koetter P., Liebl S., Louis E.J., Manus V., Mewes H.-W., Miosga T., Obermaier B., Perea J., Pohl T.M., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rasmussen S.W., Rose M., Rossau R., Schaaff-Gerstenschlaeger I., Smits P.H.M., Scarcez T., Soriano N., To Van D., Tzermia M., Van Broekhoven A., Vandenbol M., Wedler H., von Wettstein D., Wambutt R., Zagulski M., Zollner A., Karpfinger-Hartl L.
      EMBO J. 15:2031-2049(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    3. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    4. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    5. "Two-dimensional electrophoretic separation of yeast proteins using a non-linear wide range (pH 3-10) immobilized pH gradient in the first dimension; reproducibility and evidence for isoelectric focusing of alkaline (pI > 7) proteins."
      Norbeck J., Blomberg A.
      Yeast 13:1519-1534(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 64-70 AND 218-225.
      Strain: ATCC 44827 / SKQ2N.
    6. "Divergence of glyceraldehyde-3-phosphate dehydrogenase isozymes in Saccharomyces cerevisiae complex."
      Kadokura T., Ito T., Takano S., Nakazato A., Hara H., Watanabe S., Kudo T., Takeda M., Kaneko T.
      Syst. Appl. Microbiol. 23:198-205(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF N-TERMINUS.
    7. "Yeast mitochondrial dehydrogenases are associated in a supramolecular complex."
      Grandier-Vazeille X., Bathany K., Chaignepain S., Camougrand N., Manon S., Schmitter J.-M.
      Biochemistry 40:9758-9769(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBCELLULAR LOCATION.
    8. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiG3P1_YEAST
    AccessioniPrimary (citable) accession number: P00360
    Secondary accession number(s): D6VWD1
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 21, 1986
    Last sequence update: January 23, 2007
    Last modified: October 1, 2014
    This is version 157 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    There are three genes for G3PDH in yeast.
    Present with 120000 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families
    3. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    4. Yeast chromosome X
      Yeast (Saccharomyces cerevisiae) chromosome X: entries and gene names

    External Data

    Dasty 3