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Protein

Glyceraldehyde-3-phosphate dehydrogenase 2

Gene

TDH2

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

D-glyceraldehyde 3-phosphate + phosphate + NAD+ = 3-phospho-D-glyceroyl phosphate + NADH.PROSITE-ProRule annotation

Pathwayi: glycolysis

This protein is involved in step 1 of the subpathway that synthesizes pyruvate from D-glyceraldehyde 3-phosphate.
Proteins known to be involved in the 5 steps of the subpathway in this organism are:
  1. Glyceraldehyde-3-phosphate dehydrogenase 1 (TDH1), Glyceraldehyde-3-phosphate dehydrogenase 2 (TDH2), Glyceraldehyde-3-phosphate dehydrogenase 3 (TDH3)
  2. Phosphoglycerate kinase (PGK1)
  3. Phosphoglycerate mutase 2 (GPM2), Phosphoglycerate mutase 3 (GPM3), Phosphoglycerate mutase 1 (GPM1)
  4. Enolase 2 (ENO2), Enolase-related protein 2 (ERR2), Enolase-related protein 3 (ERR3), Enolase-related protein 1 (ERR1), Enolase 1 (ENO1)
  5. Pyruvate kinase 2 (PYK2), Pyruvate kinase 1 (CDC19)
This subpathway is part of the pathway glycolysis, which is itself part of Carbohydrate degradation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes pyruvate from D-glyceraldehyde 3-phosphate, the pathway glycolysis and in Carbohydrate degradation.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei33NADBy similarity1
Binding sitei78NAD; via carbonyl oxygenBy similarity1
Active sitei150NucleophilePROSITE-ProRule annotation1
Sitei177Activates thiol group during catalysisBy similarity1
Binding sitei180Glyceraldehyde 3-phosphateBy similarity1
Binding sitei232Glyceraldehyde 3-phosphateBy similarity1
Binding sitei314NADBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi11 – 12NADBy similarity2

GO - Molecular functioni

  • glyceraldehyde-3-phosphate dehydrogenase (NAD+) (phosphorylating) activity Source: SGD
  • NAD binding Source: InterPro
  • NADP binding Source: InterPro
  • promoter-specific chromatin binding Source: SGD

GO - Biological processi

  • apoptotic process Source: SGD
  • gluconeogenesis Source: SGD
  • glycolytic process Source: SGD
  • reactive oxygen species metabolic process Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Glycolysis

Keywords - Ligandi

NAD

Enzyme and pathway databases

BioCyciYEAST:YJR009C-MONOMER.
SABIO-RKP00358.
UniPathwayiUPA00109; UER00184.

Names & Taxonomyi

Protein namesi
Recommended name:
Glyceraldehyde-3-phosphate dehydrogenase 2 (EC:1.2.1.12)
Short name:
GAPDH 2
Gene namesi
Name:TDH2
Synonyms:GPD2
Ordered Locus Names:YJR009C
ORF Names:J1433
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome X

Organism-specific databases

EuPathDBiFungiDB:YJR009C.
SGDiS000003769. TDH2.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: SGD
  • fungal-type cell wall Source: SGD
  • lipid particle Source: SGD
  • mitochondrion Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved1 Publication
ChainiPRO_00001455902 – 332Glyceraldehyde-3-phosphate dehydrogenase 2Add BLAST331

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Cross-linki46Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)By similarity
Cross-linki63Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)Combined sources
Modified residuei302PhosphoserineBy similarity1

Keywords - PTMi

Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

MaxQBiP00358.
PRIDEiP00358.
TopDownProteomicsiP00358.

2D gel databases

SWISS-2DPAGEP00358.

PTM databases

iPTMnetiP00358.

Interactioni

Subunit structurei

Homotetramer.

Binary interactionsi

WithEntry#Exp.IntActNotes
UBI4P0CG632EBI-7212,EBI-7000452

Protein-protein interaction databases

BioGridi33765. 97 interactors.
DIPiDIP-1951N.
IntActiP00358. 33 interactors.
MINTiMINT-399020.

Structurei

3D structure databases

ProteinModelPortaliP00358.
SMRiP00358.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni149 – 151Glyceraldehyde 3-phosphate bindingBy similarity3
Regioni209 – 210Glyceraldehyde 3-phosphate bindingBy similarity2

Sequence similaritiesi

Phylogenomic databases

GeneTreeiENSGT00860000134940.
HOGENOMiHOG000071678.
InParanoidiP00358.
KOiK00134.
OMAiQKSATYD.
OrthoDBiEOG092C2MZH.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
InterProiIPR020831. GlycerAld/Erythrose_P_DH.
IPR020830. GlycerAld_3-P_DH_AS.
IPR020829. GlycerAld_3-P_DH_cat.
IPR020828. GlycerAld_3-P_DH_NAD(P)-bd.
IPR006424. Glyceraldehyde-3-P_DH_1.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PANTHERiPTHR10836. PTHR10836. 1 hit.
PfamiPF02800. Gp_dh_C. 1 hit.
PF00044. Gp_dh_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000149. GAP_DH. 1 hit.
PRINTSiPR00078. G3PDHDRGNASE.
SMARTiSM00846. Gp_dh_N. 1 hit.
[Graphical view]
SUPFAMiSSF51735. SSF51735. 1 hit.
TIGRFAMsiTIGR01534. GAPDH-I. 1 hit.
PROSITEiPS00071. GAPDH. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P00358-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVRVAINGFG RIGRLVMRIA LQRKNVEVVA LNDPFISNDY SAYMFKYDST
60 70 80 90 100
HGRYAGEVSH DDKHIIVDGH KIATFQERDP ANLPWASLNI DIAIDSTGVF
110 120 130 140 150
KELDTAQKHI DAGAKKVVIT APSSTAPMFV MGVNEEKYTS DLKIVSNASC
160 170 180 190 200
TTNCLAPLAK VINDAFGIEE GLMTTVHSMT ATQKTVDGPS HKDWRGGRTA
210 220 230 240 250
SGNIIPSSTG AAKAVGKVLP ELQGKLTGMA FRVPTVDVSV VDLTVKLNKE
260 270 280 290 300
TTYDEIKKVV KAAAEGKLKG VLGYTEDAVV SSDFLGDSNS SIFDAAAGIQ
310 320 330
LSPKFVKLVS WYDNEYGYST RVVDLVEHVA KA
Length:332
Mass (Da):35,847
Last modified:January 23, 2007 - v3
Checksum:i9A99DCB26672541E
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti77E → A AA sequence (PubMed:7737086).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X60157 Genomic DNA. Translation: CAA42725.1.
V01301 Genomic DNA. Translation: CAA24608.1. Sequence problems.
X87611 Genomic DNA. Translation: CAA60931.1.
Z49509 Genomic DNA. Translation: CAA89531.1.
BK006943 Genomic DNA. Translation: DAA08800.1.
PIRiS57024. DEBYG1.
RefSeqiNP_012542.1. NM_001181666.1.

Genome annotation databases

EnsemblFungiiYJR009C; YJR009C; YJR009C.
GeneIDi853465.
KEGGisce:YJR009C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X60157 Genomic DNA. Translation: CAA42725.1.
V01301 Genomic DNA. Translation: CAA24608.1. Sequence problems.
X87611 Genomic DNA. Translation: CAA60931.1.
Z49509 Genomic DNA. Translation: CAA89531.1.
BK006943 Genomic DNA. Translation: DAA08800.1.
PIRiS57024. DEBYG1.
RefSeqiNP_012542.1. NM_001181666.1.

3D structure databases

ProteinModelPortaliP00358.
SMRiP00358.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi33765. 97 interactors.
DIPiDIP-1951N.
IntActiP00358. 33 interactors.
MINTiMINT-399020.

PTM databases

iPTMnetiP00358.

2D gel databases

SWISS-2DPAGEP00358.

Proteomic databases

MaxQBiP00358.
PRIDEiP00358.
TopDownProteomicsiP00358.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYJR009C; YJR009C; YJR009C.
GeneIDi853465.
KEGGisce:YJR009C.

Organism-specific databases

EuPathDBiFungiDB:YJR009C.
SGDiS000003769. TDH2.

Phylogenomic databases

GeneTreeiENSGT00860000134940.
HOGENOMiHOG000071678.
InParanoidiP00358.
KOiK00134.
OMAiQKSATYD.
OrthoDBiEOG092C2MZH.

Enzyme and pathway databases

UniPathwayiUPA00109; UER00184.
BioCyciYEAST:YJR009C-MONOMER.
SABIO-RKP00358.

Miscellaneous databases

PROiP00358.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
InterProiIPR020831. GlycerAld/Erythrose_P_DH.
IPR020830. GlycerAld_3-P_DH_AS.
IPR020829. GlycerAld_3-P_DH_cat.
IPR020828. GlycerAld_3-P_DH_NAD(P)-bd.
IPR006424. Glyceraldehyde-3-P_DH_1.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PANTHERiPTHR10836. PTHR10836. 1 hit.
PfamiPF02800. Gp_dh_C. 1 hit.
PF00044. Gp_dh_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000149. GAP_DH. 1 hit.
PRINTSiPR00078. G3PDHDRGNASE.
SMARTiSM00846. Gp_dh_N. 1 hit.
[Graphical view]
SUPFAMiSSF51735. SSF51735. 1 hit.
TIGRFAMsiTIGR01534. GAPDH-I. 1 hit.
PROSITEiPS00071. GAPDH. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiG3P2_YEAST
AccessioniPrimary (citable) accession number: P00358
Secondary accession number(s): D6VWI4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: January 23, 2007
Last modified: November 30, 2016
This is version 177 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

There are three genes for G3PDH in yeast.
Present with 121000 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome X
    Yeast (Saccharomyces cerevisiae) chromosome X: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.