Glyceraldehyde-3-phosphate dehydrogenase 2 - Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Contribute

Send feedback

Read comments (?) or add your own

P00358 (G3P2_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified December 14, 2011. Version 132. History...

Clusters with 100%, 90%, 50% identity |

Documents (4) |

Third-party data

text xml rdf/xml gff fasta

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Glyceraldehyde-3-phosphate dehydrogenase 2
Short name=GAPDH 2
EC=1.2.1.12

Gene names

Name:	TDH2
Synonyms:	GPD2
Ordered Locus Names:	YJR009C
ORF Names:	J1433

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	332 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Catalytic activity	D-glyceraldehyde 3-phosphate + phosphate + NAD⁺ = 3-phospho-D-glyceroyl phosphate + NADH.
Pathway	Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 1/5.
Subunit structure	Homotetramer.
Subcellular location	Cytoplasm.
Miscellaneous	There are three genes for G3PDH in yeast. Present with 121000 molecules/cell in log phase SD medium. Ref.7
Sequence similarities	Belongs to the glyceraldehyde-3-phosphate dehydrogenase family.

Ontologies

Keywords
Biological process	Glycolysis
Cellular component	Cytoplasm
Ligand	NAD
Molecular function	Oxidoreductase
PTM	Phosphoprotein
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological process	apoptotic process Inferred from mutant phenotype. Source: SGD gluconeogenesis Inferred from expression pattern. Source: SGD glycolysis Inferred from expression pattern. Source: SGD reactive oxygen species metabolic process Inferred from mutant phenotype. Source: SGD
Cellular component	fungal-type cell wall Inferred from direct assay. Source: SGD lipid particle Inferred from direct assay. Source: SGD mitochondrion Inferred from direct assay. Source: SGD plasma membrane enriched fraction Inferred from direct assay. Source: SGD
Molecular function	NAD binding Inferred from electronic annotation. Source: InterPro NADP binding Inferred from electronic annotation. Source: InterPro glyceraldehyde-3-phosphate dehydrogenase (NAD+) (phosphorylating) activity Inferred from direct assay. Source: SGD
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Initiator methionine

Removed Ref.6

Chain

2 – 332

331

Glyceraldehyde-3-phosphate dehydrogenase 2

PRO_0000145590

Regions

Nucleotide binding

11 – 12

NAD By similarity

Region

149 – 151

Glyceraldehyde 3-phosphate binding By similarity

Region

209 – 210

Glyceraldehyde 3-phosphate binding By similarity

Sites

Active site

150

Nucleophile By similarity

Binding site

NAD By similarity

Binding site

NAD; via carbonyl oxygen By similarity

Binding site

180

Glyceraldehyde 3-phosphate By similarity

Binding site

232

Glyceraldehyde 3-phosphate By similarity

Binding site

314

NAD By similarity

Site

177

Activates thiol group during catalysis By similarity

Amino acid modifications

Modified residue

Phosphoserine Ref.9

Modified residue

Phosphothreonine Ref.9

Modified residue

Phosphotyrosine Ref.9

Modified residue

Phosphoserine Ref.9

Modified residue

149

Phosphoserine Ref.8

Modified residue

201

Phosphoserine Ref.8

Modified residue

310

Phosphoserine Ref.9

Modified residue

319

Phosphoserine Ref.9

Modified residue

320

Phosphothreonine Ref.9

Experimental info

Sequence conflict

E → A AA sequence Ref.5

Sequences

Sequence

Length

Mass (Da)

Tools

P00358 [UniParc].

Last modified January 23, 2007. Version 3.
Checksum: 9A99DCB26672541E
Show »

FASTA

332

35,847

        10         20         30         40         50         60 
MVRVAINGFG RIGRLVMRIA LQRKNVEVVA LNDPFISNDY SAYMFKYDST HGRYAGEVSH 

        70         80         90        100        110        120 
DDKHIIVDGH KIATFQERDP ANLPWASLNI DIAIDSTGVF KELDTAQKHI DAGAKKVVIT 

       130        140        150        160        170        180 
APSSTAPMFV MGVNEEKYTS DLKIVSNASC TTNCLAPLAK VINDAFGIEE GLMTTVHSMT 

       190        200        210        220        230        240 
ATQKTVDGPS HKDWRGGRTA SGNIIPSSTG AAKAVGKVLP ELQGKLTGMA FRVPTVDVSV 

       250        260        270        280        290        300 
VDLTVKLNKE TTYDEIKKVV KAAAEGKLKG VLGYTEDAVV SSDFLGDSNS SIFDAAAGIQ 

       310        320        330 
LSPKFVKLVS WYDNEYGYST RVVDLVEHVA KA

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"TDH2 is linked to MET3 on chromosome X of Saccharomyces cerevisiae." Mountain H.A., Korch C. Yeast 7:873-880(1991) [PubMed: 1789010] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[2]	"Structural comparison of two nontandemly repeated yeast glyceraldehyde-3-phosphate dehydrogenase genes." Holland J.P., Holland M.J. J. Biol. Chem. 255:2596-2605(1980) [PubMed: 6244283] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"Complete nucleotide sequence of Saccharomyces cerevisiae chromosome X." Galibert F., Alexandraki D., Baur A., Boles E., Chalwatzis N., Chuat J.-C., Coster F., Cziepluch C., de Haan M., Domdey H., Durand P., Entian K.-D., Gatius M., Goffeau A., Grivell L.A., Hennemann A., Herbert C.J., Heumann K. Karpfinger-Hartl L. EMBO J. 15:2031-2049(1996) [PubMed: 8641269] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[4]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[5]	"Gene linkage of two-dimensional polyacrylamide gel electrophoresis resolved proteins from isogene families in Saccharomyces cerevisiae by microsequencing of in-gel trypsin generated peptides." Norbeck J., Blomberg A. Electrophoresis 16:149-156(1995) [PubMed: 7737086] [Abstract] Cited for: PROTEIN SEQUENCE OF 24-37; 72-77; 81-86; 199-213; 226-232 AND 322-331. Strain: ATCC 38531 / Y41.
[6]	Frutiger S., Hughes G.J., Sanchez J.-C., Hochstrasser D.F. Submitted (FEB-1996) to UniProtKB Cited for: PROTEIN SEQUENCE OF 2-13. Strain: ATCC 26786 / X2180-1A.
[7]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[8]	"Quantitative phosphoproteomics applied to the yeast pheromone signaling pathway." Gruhler A., Olsen J.V., Mohammed S., Mortensen P., Faergeman N.J., Mann M., Jensen O.N. Mol. Cell. Proteomics 4:310-327(2005) [PubMed: 15665377] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-149 AND SER-201, MASS SPECTROMETRY. Strain: YAL6B.
[9]	"Analysis of phosphorylation sites on proteins from Saccharomyces cerevisiae by electron transfer dissociation (ETD) mass spectrometry." Chi A., Huttenhower C., Geer L.Y., Coon J.J., Syka J.E.P., Bai D.L., Shabanowitz J., Burke D.J., Troyanskaya O.G., Hunt D.F. Proc. Natl. Acad. Sci. U.S.A. 104:2193-2198(2007) [PubMed: 17287358] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-49; THR-50; TYR-54; SER-59; SER-310; SER-319 AND THR-320, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X60157 Genomic DNA. Translation: CAA42725.1. V01301 Genomic DNA. Translation: CAA24608.1. Sequence problems. X87611 Genomic DNA. Translation: CAA60931.1. Z49509 Genomic DNA. Translation: CAA89531.1. BK006943 Genomic DNA. Translation: DAA08800.1.
PIR	DEBYG1. S57024.
RefSeq	NP_012542.1. NM_001181666.1.
3D structure databases
ProteinModelPortal	P00358.
SMR	P00358. Positions 1-330.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-1951N.
IntAct	P00358. 27 interactions.
MINT	MINT-399020.
STRING	P00358.
2D gel databases
SWISS-2DPAGE	P00358.
Proteomic databases
PeptideAtlas	P00358.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YJR009C; YJR009C; YJR009C.
GeneID	853465.
KEGG	sce:YJR009C.
NMPDR	fig\|4932.3.peg.3516.
Organism-specific databases
SGD	S000003769. TDH2.
Phylogenomic databases
eggNOG	fuNOG04196.
GeneTree	EFGT00050000004847.
HOGENOM	HBG571736.
OMA	CSINEIS.
OrthoDB	EOG4578GC.
Gene expression databases
Genevestigator	P00358.
GermOnline	YJR009C. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR020831. GlycerAld/Erythrose_P_DH. IPR020830. GlycerAld_3-P_DH_AS. IPR020829. GlycerAld_3-P_DH_cat. IPR020828. GlycerAld_3-P_DH_NAD(P)-bd. IPR006424. Glyceraldehyde-3-P_DH_1. IPR016040. NAD(P)-bd_dom. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
KO	K00134.
PANTHER	PTHR10836. GAP_DH. 1 hit.
Pfam	PF02800. Gp_dh_C. 1 hit. PF00044. Gp_dh_N. 1 hit. [Graphical view]
PIRSF	PIRSF000149. GAP_DH. 1 hit.
PRINTS	PR00078. G3PDHDRGNASE.
SMART	SM00846. Gp_dh_N. 1 hit. [Graphical view]
TIGRFAMs	TIGR01534. GAPDH-I. 1 hit.
PROSITE	PS00071. GAPDH. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	974053.

Entry information

Entry name

G3P2_YEAST

Accession

Primary (citable) accession number: P00358
Secondary accession number(s): D6VWI4

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 21, 1986
Last sequence update:	January 23, 2007
Last modified:	December 14, 2011
This is version 132 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program

Relevant documents

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

Yeast chromosome X

Yeast (Saccharomyces cerevisiae) chromosome X: entries and gene names

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Sites

Amino acid modifications

Experimental info

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

2D gel databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents