Reviewed, UniProtKB/Swiss-Prot P00349 (6PGD_SHEEP)

Names and origin

Protein names

Recommended name:
    6-phosphogluconate dehydrogenase, decarboxylating
    EC=1.1.1.44

Gene names

Name:

PGD

Organism

Ovis aries (Sheep)

Taxonomic identifier

9940 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Cetartiodactyla › Ruminantia › Pecora › Bovidae › Caprinae › Ovis

Protein attributes

Sequence length	483 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Catalytic activity	6-phospho-D-gluconate + NADP+ = D-ribulose 5-phosphate + CO2 + NADPH.
Pathway	Carbohydrate degradation; pentose phosphate pathway; D-ribulose 5-phosphate from D-glucose 6-phosphate (oxidative stage): step 3/3.
Subunit structure	Homodimer.
Sequence similarities	Belongs to the 6-phosphogluconate dehydrogenase family.
Sequence caution	The sequence described in Ref.2 differs from that shown. Reason: Miscellaneous discrepancy. Incorrect CNBR peptide order. Many sequencing errors.

Ontologies

Keywords
Biological process	Pentose shunt
Ligand	NADP
Molecular function	Oxidoreductase
Technical term	3D-structure Direct protein sequencing
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW pentose-phosphate shunt Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	NADP or NADPH binding Inferred from electronic annotation. Source: InterPro phosphogluconate dehydrogenase (decarboxylating) activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Initiator methionine

1

1

Removed Ref.2

Chain

2 – 483

482

6-phosphogluconate dehydrogenase, decarboxylating

PRO_0000090066

Secondary structure

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483

Helix Strand Turn

Details...

Beta strand

4 – 9

6

Helix

13 – 24

12

Beta strand

29 – 32

4

Helix

37 – 44

8

Turn

45 – 49

5

Helix

58 – 64

7

Beta strand

70 – 73

4

Helix

79 – 91

13

Beta strand

97 – 100

4

Helix

106 – 118

13

Beta strand

122 – 130

9

Helix

131 – 137

7

Beta strand

140 – 145

6

Helix

147 – 149

3

Helix

150 – 160

11

Turn

165 – 167

3

Beta strand

168 – 170

3

Helix

179 – 207

29

Helix

213 – 223

11

Turn

224 – 228

5

Helix

231 – 241

11

Beta strand

247 – 250

4

Helix

251 – 253

3

Helix

263 – 274

12

Helix

279 – 292

14

Helix

294 – 303

10

Helix

316 – 349

34

Helix

355 – 361

7

Beta strand

364 – 366

3

Helix

372 – 382

11

Helix

389 – 391

3

Helix

393 – 416

24

Helix

421 – 434

14

Helix

440 – 451

12

Beta strand

457 – 460

4

Sequences

	Sequence		Length	Mass (Da)
	P00349-1 [UniParc]. Last modified January 23, 2007. Version 4. Checksum: F4AC5DB414385F11	FASTA	483	52,970
	10 20 30 40 50 60 MAQADIALIG LAVMGQNLIL NMNDHGFVVC AFNRTVSKVD DFLANEAKGT KVLGAHSLEE 70 80 90 100 110 120 MVSKLKKPRR IILLVKAGQA VDNFIEKLVP LLDIGDIIID GGNSEYRDTM RRCRDLKDKG 130 140 150 160 170 180 ILFVGSGVSG GEDGARYGPS LMPGGNKEAW PHIKAIFQGI AAKVGTGEPC CDWVGDDGAG 190 200 210 220 230 240 HFVKMVHNGI EYGDMQLICE AYHLMKDVLG LGHKEMAKAF EEWNKTELDS FLIEITASIL 250 260 270 280 290 300 KFQDADGKHL LPKIRDSAGQ KGTGKWTAIS ALEYGVPVTL IGEAVFARCL SSLKDERIQA 310 320 330 340 350 360 SKKLKGPQNI PFEGDKKSFL EDIRKALYAS KIISYAQGFM LLRQAATEFG WTLNYGGIAL 370 380 390 400 410 420 MWRGGCIIRS VFLGKIKDAF DRNPGLQNLL LDDFFKSAVE NCQDSWRRAI STGVQAGIPM 430 440 450 460 470 480 PCFTTALSFY DGYRHAMLPA NLIQAQRDYF GAHTYELLAK PGQFIHTNWT GHGGSVSSSS YNA

References

[1]	"Sheep 6-phosphogluconate dehydrogenase. Revised protein sequence based upon the sequences of cDNA clones obtained with the polymerase chain reaction." Somers D.O., Medd S.M., Walker J.E., Adams M.J. Biochem. J. 288:1061-1067(1992) [PubMed: 1471978] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Liver.
[2]	"Amino acid sequence of ovine 6-phosphogluconate dehydrogenase." Carne A., Walker J.E. J. Biol. Chem. 258:12895-12906(1983) [PubMed: 6685125] [Abstract] Cited for: PRELIMINARY PROTEIN SEQUENCE OF 2-483. Tissue: Liver.
[3]	"The three dimensional structure of sheep liver 6-phosphogluconate dehydrogenase at 2.6-A resolution." Adams M.J., Archibald I.G., Bugg C.E., Carne A., Gover S., Helliwell J.R., Pickersgill R.W., White S.W. EMBO J. 2:1009-1014(1983) [PubMed: 6641716] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.6 ANGSTROMS).
[4]	"The structure of 6-phosphogluconate dehydrogenase refined at 2.5-A resolution." Adams M.J., Gover S., Leaback R., Phillips C., Somers D.O. Acta Crystallogr. B 47:817-820(1991) [PubMed: 1793548] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.5 ANGSTROMS).
[5]	"Crystallographic study of coenzyme, coenzyme analogue and substrate binding in 6-phosphogluconate dehydrogenase: implications for NADP specificity and the enzyme mechanism." Adams M.J., Ellis G.H., Gover S., Naylor C.E., Phillips C. Structure 2:651-668(1994) [PubMed: 7922042] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.3 ANGSTROMS).
[6]	"Structure of 6-phosphogluconate dehydrogenase refined at 2-A resolution." Phillips C., Gover S., Adams M.J. Acta Crystallogr. D 51:290-304(1995) [PubMed: 15299295] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.0 ANGSTROMS).

Cross-references

Sequence databases

EMBLGenBankDDBJ

X60195 mRNA. Translation: CAA42751.1.

PIR

S15280.
DESHGC. S27359.

RefSeq

NP_001009467.1.

UniGene

Oar.419

3D structure databases

PDBPDBe

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1PGN	X-ray	2.30	A	2-483	[»]
1PGO	X-ray	2.50	A	2-483	[»]
1PGP	X-ray	2.50	A	2-483	[»]
1PGQ	X-ray	3.17	A	2-483	[»]
2PGD	X-ray	2.00	A	2-483	[»]

ModBase

Search...

Genome annotation databases

GeneID

443541.

Phylogenomic databases

HOVERGEN

P00349.

Enzyme and pathway databases

BRENDA

1.1.1.44. 271.

Family and domain databases

InterPro

IPR006183. 6-phosphogluconate_DH.
IPR006114. 6PGDH_C.
IPR006113. 6PGDH_decarbox.
IPR006115. 6PGDH_NAD-bd.
IPR006184. 6PGdom_BS.
IPR013328. DH_multihelical.
IPR012284. Fibritin/6PGD_C-extension.
IPR016040. NAD(P)-bd_dom.
[Graphical view]

Gene3D

G3DSA:1.20.5.320. Fibritin/6PGD_C-extension. 1 hit.
G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
G3DSA:1.10.1040.10. Opine_DH. 1 hit.

Pfam

PF00393. 6PGD. 1 hit.
PF03446. NAD_binding_2. 1 hit.
[Graphical view]

PRINTS

PR00076. 6PGDHDRGNASE.

TIGRFAMs

TIGR00873. gnd. 1 hit.

PROSITE

PS00461. 6PGD. 1 hit.
[Graphical view]

ProtoNet

Search...

Entry information

Entry name

6PGD_SHEEP

Accession

Primary (citable) accession number: P00349

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 21, 1986
Last sequence update:	January 23, 2007
Last modified:	June 16, 2009
This is version 89 of the entry and version 4 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)