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Protein

L-lactate dehydrogenase

Gene

ldh

Organism
Bacillus megaterium
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

(S)-lactate + NAD+ = pyruvate + NADH.

Pathwayi: pyruvate fermentation to lactate

This protein is involved in step 1 of the subpathway that synthesizes (S)-lactate from pyruvate.
Proteins known to be involved in this subpathway in this organism are:
  1. L-lactate dehydrogenase (ldh), L-lactate dehydrogenase (ldh), L-lactate dehydrogenase (ldh), L-lactate dehydrogenase (ldh), L-lactate dehydrogenase (ldh), L-lactate dehydrogenase (ldh), L-lactate dehydrogenase (ldh), L-lactate dehydrogenase (ldh), L-lactate dehydrogenase (ldh), L-lactate dehydrogenase (ldh), L-lactate dehydrogenase (ldh)
This subpathway is part of the pathway pyruvate fermentation to lactate, which is itself part of Fermentation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes (S)-lactate from pyruvate, the pathway pyruvate fermentation to lactate and in Fermentation.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei94SubstrateBy similarity1
Binding sitei126NAD or substrateBy similarity1
Binding sitei157SubstrateBy similarity1
Active sitei181Proton acceptorBy similarity1
Binding sitei235SubstrateBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi18 – 46NADBy similarityAdd BLAST29

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionOxidoreductase
LigandNAD

Enzyme and pathway databases

BRENDAi1.1.1.27. 656.
UniPathwayiUPA00554; UER00611.

Names & Taxonomyi

Protein namesi
Recommended name:
L-lactate dehydrogenase (EC:1.1.1.27)
Short name:
L-LDH
Gene namesi
Name:ldh
OrganismiBacillus megaterium
Taxonomic identifieri1404 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001683251 – 318L-lactate dehydrogenaseAdd BLAST318

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei226PhosphotyrosineBy similarity1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PRIDEiP00345.

Interactioni

Subunit structurei

Homotetramer.

Structurei

3D structure databases

ProteinModelPortaliP00345.
SMRiP00345.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the LDH/MDH superfamily. LDH family.Curated

Phylogenomic databases

eggNOGiENOG4105C80. Bacteria.
COG0039. LUCA.

Family and domain databases

Gene3Di3.90.110.10. 1 hit.
HAMAPiMF_00488. Lactate_dehydrog. 1 hit.
InterProiView protein in InterPro
IPR001557. L-lactate/malate_DH.
IPR011304. L-lactate_DH.
IPR018177. L-lactate_DH_AS.
IPR022383. Lactate/malate_DH_C.
IPR001236. Lactate/malate_DH_N.
IPR015955. Lactate_DH/Glyco_Ohase_4_C.
IPR036291. NAD(P)-bd_dom_sf.
PfamiView protein in Pfam
PF02866. Ldh_1_C. 1 hit.
PF00056. Ldh_1_N. 1 hit.
PIRSFiPIRSF000102. Lac_mal_DH. 1 hit.
PRINTSiPR00086. LLDHDRGNASE.
SUPFAMiSSF51735. SSF51735. 1 hit.
SSF56327. SSF56327. 1 hit.
TIGRFAMsiTIGR01771. L-LDH-NAD. 1 hit.
PROSITEiView protein in PROSITE
PS00064. L_LDH. 1 hit.

Sequencei

Sequence statusi: Complete.

P00345-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKTQFTPKTR KVAVIGTGFV GSSYAFSMVN QGIANELVLI DMNKEKAEGE
60 70 80 90 100
ARDINHGMPF ATPMKIWAGD YKDCADADLA VITAGANQAP GETRLDLVEK
110 120 130 140 150
NVKIFECIVK DIMNSGFDGI ILVATNPVDI LAHVTQKVSG LPNGRVIGSG
160 170 180 190 200
TILDTARFRY LLSDYFEVDS RNVHAYIMGE HGDTEFPVWS HAQIGGVKLE
210 220 230 240 250
HFINTAAIEK EPDMQHLFEQ TRDAAYHIIN RKGATYYGIA MGLVRITKAI
260 270 280 290 300
LDDENSILTV SALLEGQYGI SDVYIGVPAI INKNGVRQII ELNLTPHEQQ
310
QLEHSASILK QTRDRAFV
Length:318
Mass (Da):35,035
Last modified:January 1, 1990 - v2
Checksum:i17122190576E1485
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M22305 Genomic DNA. Translation: AAA22566.1.
PIRiS00133. DEBSLM.

Similar proteinsi

Entry informationi

Entry nameiLDH_BACME
AccessioniPrimary (citable) accession number: P00345
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: January 1, 1990
Last modified: October 25, 2017
This is version 112 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Direct protein sequencing

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families