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Protein

L-lactate dehydrogenase

Gene

ldh

Organism
Lactobacillus casei
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

(S)-lactate + NAD+ = pyruvate + NADH.

Pathwayi: pyruvate fermentation to lactate

This protein is involved in step 1 of the subpathway that synthesizes (S)-lactate from pyruvate.
Proteins known to be involved in this subpathway in this organism are:
  1. L-lactate dehydrogenase (ldh), L-lactate dehydrogenase (ldh), L-lactate dehydrogenase (ldh), L-lactate dehydrogenase (ldh), L-lactate dehydrogenase (ldh), L-lactate dehydrogenase (ldh)
This subpathway is part of the pathway pyruvate fermentation to lactate, which is itself part of Fermentation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes (S)-lactate from pyruvate, the pathway pyruvate fermentation to lactate and in Fermentation.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei94SubstrateBy similarity1
Binding sitei126NAD or substrateBy similarity1
Binding sitei157SubstrateBy similarity1
Active sitei181Proton acceptorBy similarity1
Binding sitei235SubstrateBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi18 – 46NADBy similarityAdd BLAST29

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Ligandi

NAD

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-8681.
SABIO-RKP00343.
UniPathwayiUPA00554; UER00611.

Names & Taxonomyi

Protein namesi
Recommended name:
L-lactate dehydrogenase (EC:1.1.1.27)
Short name:
L-LDH
Gene namesi
Name:ldh
OrganismiLactobacillus casei
Taxonomic identifieri1582 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliLactobacillalesLactobacillaceaeLactobacillus

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved1 Publication
ChainiPRO_00001683472 – 326L-lactate dehydrogenaseAdd BLAST325

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei226PhosphotyrosineBy similarity1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PRIDEiP00343.

Interactioni

Subunit structurei

Homotetramer.

Protein-protein interaction databases

STRINGi543734.LCABL_27160.

Structurei

Secondary structure

1326
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi4 – 6Combined sources3
Beta strandi11 – 15Combined sources5
Helixi19 – 31Combined sources13
Beta strandi35 – 40Combined sources6
Helixi44 – 55Combined sources12
Helixi56 – 60Combined sources5
Beta strandi65 – 68Combined sources4
Helixi71 – 74Combined sources4
Beta strandi78 – 82Combined sources5
Beta strandi91 – 93Combined sources3
Helixi94 – 103Combined sources10
Helixi105 – 113Combined sources9
Turni114 – 116Combined sources3
Beta strandi119 – 123Combined sources5
Beta strandi125 – 127Combined sources3
Helixi128 – 139Combined sources12
Helixi143 – 145Combined sources3
Beta strandi146 – 148Combined sources3
Helixi152 – 166Combined sources15
Helixi170 – 172Combined sources3
Beta strandi177 – 182Combined sources6
Helixi189 – 191Combined sources3
Helixi199 – 205Combined sources7
Helixi211 – 232Combined sources22
Helixi237 – 251Combined sources15
Beta strandi256 – 266Combined sources11
Helixi267 – 269Combined sources3
Beta strandi270 – 282Combined sources13
Beta strandi285 – 289Combined sources5
Helixi296 – 310Combined sources15
Helixi313 – 316Combined sources4
Helixi318 – 323Combined sources6

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1LLCX-ray3.00A2-326[»]
2ZQYX-ray2.60A/B/C/D1-326[»]
2ZQZX-ray2.50A/B/C/D/E/F1-326[»]
3VKUX-ray1.96A/B/C/D/E/F1-326[»]
3VKVX-ray2.70A/B/C/D/E/F1-326[»]
3VPFX-ray2.79A/B/C/D/E/F1-326[»]
ProteinModelPortaliP00343.
SMRiP00343.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP00343.

Family & Domainsi

Sequence similaritiesi

Belongs to the LDH/MDH superfamily. LDH family.Curated

Phylogenomic databases

eggNOGiENOG4105C80. Bacteria.
COG0039. LUCA.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
3.90.110.10. 1 hit.
HAMAPiMF_00488. Lactate_dehydrog. 1 hit.
InterProiIPR001557. L-lactate/malate_DH.
IPR011304. L-lactate_DH.
IPR018177. L-lactate_DH_AS.
IPR022383. Lactate/malate_DH_C.
IPR001236. Lactate/malate_DH_N.
IPR015955. Lactate_DH/Glyco_Ohase_4_C.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PANTHERiPTHR11540. PTHR11540. 1 hit.
PfamiPF02866. Ldh_1_C. 1 hit.
PF00056. Ldh_1_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000102. Lac_mal_DH. 1 hit.
PRINTSiPR00086. LLDHDRGNASE.
SUPFAMiSSF51735. SSF51735. 1 hit.
SSF56327. SSF56327. 1 hit.
TIGRFAMsiTIGR01771. L-LDH-NAD. 1 hit.
PROSITEiPS00064. L_LDH. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P00343-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MASITDKDHQ KVILVGDGAV GSSYAYAMVL QGIAQEIGIV DIFKDKTKGD
60 70 80 90 100
AIDLSNALPF TSPKKIYSAE YSDAKDADLV VITAGAPQKP GETRLDLVNK
110 120 130 140 150
NLKILKSIVD PIVDSGFNGI FLVAANPVDI LTYATWKLSG FPKNRVVGSG
160 170 180 190 200
TSLDTARFRQ SIAEMVNVDA RSVHAYIMGE HGDTEFPVWS HANIGGVTIA
210 220 230 240 250
EWVKAHPEIK EDKLVKMFED VRDAAYEIIK LKGATFYGIA TALARISKAI
260 270 280 290 300
LNDENAVLPL SVYMDGQYGL NDIYIGTPAV INRNGIQNIL EIPLTDHEEE
310 320
SMQKSASQLK KVLTDAFAKN DIETRQ
Length:326
Mass (Da):35,531
Last modified:January 23, 2007 - v3
Checksum:i934905641592AF8A
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti26Y → F AA sequence (PubMed:6411465).Curated1
Sequence conflicti52I → T AA sequence (PubMed:6411465).Curated1
Sequence conflicti88 – 89QK → KQ AA sequence (PubMed:6411465).Curated2
Sequence conflicti119G → L AA sequence (PubMed:6411465).Curated1
Sequence conflicti270L → I AA sequence (PubMed:6411465).Curated1
Sequence conflicti273I → L AA sequence (PubMed:6411465).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D12591 Genomic DNA. Translation: BAA02133.1.
M76708 Genomic DNA. Translation: AAA25245.2.
PIRiA43944. DELBLA.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D12591 Genomic DNA. Translation: BAA02133.1.
M76708 Genomic DNA. Translation: AAA25245.2.
PIRiA43944. DELBLA.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1LLCX-ray3.00A2-326[»]
2ZQYX-ray2.60A/B/C/D1-326[»]
2ZQZX-ray2.50A/B/C/D/E/F1-326[»]
3VKUX-ray1.96A/B/C/D/E/F1-326[»]
3VKVX-ray2.70A/B/C/D/E/F1-326[»]
3VPFX-ray2.79A/B/C/D/E/F1-326[»]
ProteinModelPortaliP00343.
SMRiP00343.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi543734.LCABL_27160.

Proteomic databases

PRIDEiP00343.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Phylogenomic databases

eggNOGiENOG4105C80. Bacteria.
COG0039. LUCA.

Enzyme and pathway databases

UniPathwayiUPA00554; UER00611.
BioCyciMetaCyc:MONOMER-8681.
SABIO-RKP00343.

Miscellaneous databases

EvolutionaryTraceiP00343.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
3.90.110.10. 1 hit.
HAMAPiMF_00488. Lactate_dehydrog. 1 hit.
InterProiIPR001557. L-lactate/malate_DH.
IPR011304. L-lactate_DH.
IPR018177. L-lactate_DH_AS.
IPR022383. Lactate/malate_DH_C.
IPR001236. Lactate/malate_DH_N.
IPR015955. Lactate_DH/Glyco_Ohase_4_C.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PANTHERiPTHR11540. PTHR11540. 1 hit.
PfamiPF02866. Ldh_1_C. 1 hit.
PF00056. Ldh_1_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000102. Lac_mal_DH. 1 hit.
PRINTSiPR00086. LLDHDRGNASE.
SUPFAMiSSF51735. SSF51735. 1 hit.
SSF56327. SSF56327. 1 hit.
TIGRFAMsiTIGR01771. L-LDH-NAD. 1 hit.
PROSITEiPS00064. L_LDH. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiLDH_LACCA
AccessioniPrimary (citable) accession number: P00343
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: January 23, 2007
Last modified: November 2, 2016
This is version 133 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Direct protein sequencing

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.