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Protein

L-lactate dehydrogenase

Gene

ldh

Organism
Lactobacillus casei
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

(S)-lactate + NAD+ = pyruvate + NADH.

Pathwayi: pyruvate fermentation to lactate

This protein is involved in step 1 of the subpathway that synthesizes (S)-lactate from pyruvate.
Proteins known to be involved in this subpathway in this organism are:
  1. L-lactate dehydrogenase (ldh), L-lactate dehydrogenase (ldh), L-lactate dehydrogenase (ldh), L-lactate dehydrogenase (ldh), L-lactate dehydrogenase (ldh), L-lactate dehydrogenase (ldh)
This subpathway is part of the pathway pyruvate fermentation to lactate, which is itself part of Fermentation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes (S)-lactate from pyruvate, the pathway pyruvate fermentation to lactate and in Fermentation.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei94 – 941SubstrateBy similarity
Binding sitei126 – 1261NAD or substrateBy similarity
Binding sitei157 – 1571SubstrateBy similarity
Active sitei181 – 1811Proton acceptorBy similarity
Binding sitei235 – 2351SubstrateBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi18 – 4629NADBy similarityAdd
BLAST

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Ligandi

NAD

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-8681.
SABIO-RKP00343.
UniPathwayiUPA00554; UER00611.

Names & Taxonomyi

Protein namesi
Recommended name:
L-lactate dehydrogenase (EC:1.1.1.27)
Short name:
L-LDH
Gene namesi
Name:ldh
OrganismiLactobacillus casei
Taxonomic identifieri1582 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliLactobacillalesLactobacillaceaeLactobacillus

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemoved1 Publication
Chaini2 – 326325L-lactate dehydrogenasePRO_0000168347Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei226 – 2261PhosphotyrosineBy similarity

Keywords - PTMi

Phosphoprotein

Interactioni

Subunit structurei

Homotetramer.

Protein-protein interaction databases

STRINGi543734.LCABL_27160.

Structurei

Secondary structure

1
326
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi4 – 63Combined sources
Beta strandi11 – 155Combined sources
Helixi19 – 3113Combined sources
Beta strandi35 – 406Combined sources
Helixi44 – 5512Combined sources
Helixi56 – 605Combined sources
Beta strandi65 – 684Combined sources
Helixi71 – 744Combined sources
Beta strandi78 – 825Combined sources
Beta strandi91 – 933Combined sources
Helixi94 – 10310Combined sources
Helixi105 – 1139Combined sources
Turni114 – 1163Combined sources
Beta strandi119 – 1235Combined sources
Beta strandi125 – 1273Combined sources
Helixi128 – 13912Combined sources
Helixi143 – 1453Combined sources
Beta strandi146 – 1483Combined sources
Helixi152 – 16615Combined sources
Helixi170 – 1723Combined sources
Beta strandi177 – 1826Combined sources
Helixi189 – 1913Combined sources
Helixi199 – 2057Combined sources
Helixi211 – 23222Combined sources
Helixi237 – 25115Combined sources
Beta strandi256 – 26611Combined sources
Helixi267 – 2693Combined sources
Beta strandi270 – 28213Combined sources
Beta strandi285 – 2895Combined sources
Helixi296 – 31015Combined sources
Helixi313 – 3164Combined sources
Helixi318 – 3236Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1LLCX-ray3.00A2-326[»]
2ZQYX-ray2.60A/B/C/D1-326[»]
2ZQZX-ray2.50A/B/C/D/E/F1-326[»]
3VKUX-ray1.96A/B/C/D/E/F1-326[»]
3VKVX-ray2.70A/B/C/D/E/F1-326[»]
3VPFX-ray2.79A/B/C/D/E/F1-326[»]
ProteinModelPortaliP00343.
SMRiP00343. Positions 2-321.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP00343.

Family & Domainsi

Sequence similaritiesi

Belongs to the LDH/MDH superfamily. LDH family.Curated

Phylogenomic databases

eggNOGiENOG4105C80. Bacteria.
COG0039. LUCA.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
3.90.110.10. 1 hit.
HAMAPiMF_00488. Lactate_dehydrog. 1 hit.
InterProiIPR001557. L-lactate/malate_DH.
IPR011304. L-lactate_DH.
IPR018177. L-lactate_DH_AS.
IPR022383. Lactate/malate_DH_C.
IPR001236. Lactate/malate_DH_N.
IPR015955. Lactate_DH/Glyco_Ohase_4_C.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PANTHERiPTHR11540. PTHR11540. 1 hit.
PfamiPF02866. Ldh_1_C. 1 hit.
PF00056. Ldh_1_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000102. Lac_mal_DH. 1 hit.
PRINTSiPR00086. LLDHDRGNASE.
SUPFAMiSSF51735. SSF51735. 1 hit.
SSF56327. SSF56327. 1 hit.
TIGRFAMsiTIGR01771. L-LDH-NAD. 1 hit.
PROSITEiPS00064. L_LDH. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P00343-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MASITDKDHQ KVILVGDGAV GSSYAYAMVL QGIAQEIGIV DIFKDKTKGD
60 70 80 90 100
AIDLSNALPF TSPKKIYSAE YSDAKDADLV VITAGAPQKP GETRLDLVNK
110 120 130 140 150
NLKILKSIVD PIVDSGFNGI FLVAANPVDI LTYATWKLSG FPKNRVVGSG
160 170 180 190 200
TSLDTARFRQ SIAEMVNVDA RSVHAYIMGE HGDTEFPVWS HANIGGVTIA
210 220 230 240 250
EWVKAHPEIK EDKLVKMFED VRDAAYEIIK LKGATFYGIA TALARISKAI
260 270 280 290 300
LNDENAVLPL SVYMDGQYGL NDIYIGTPAV INRNGIQNIL EIPLTDHEEE
310 320
SMQKSASQLK KVLTDAFAKN DIETRQ
Length:326
Mass (Da):35,531
Last modified:January 23, 2007 - v3
Checksum:i934905641592AF8A
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti26 – 261Y → F AA sequence (PubMed:6411465).Curated
Sequence conflicti52 – 521I → T AA sequence (PubMed:6411465).Curated
Sequence conflicti88 – 892QK → KQ AA sequence (PubMed:6411465).Curated
Sequence conflicti119 – 1191G → L AA sequence (PubMed:6411465).Curated
Sequence conflicti270 – 2701L → I AA sequence (PubMed:6411465).Curated
Sequence conflicti273 – 2731I → L AA sequence (PubMed:6411465).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D12591 Genomic DNA. Translation: BAA02133.1.
M76708 Genomic DNA. Translation: AAA25245.2.
PIRiA43944. DELBLA.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D12591 Genomic DNA. Translation: BAA02133.1.
M76708 Genomic DNA. Translation: AAA25245.2.
PIRiA43944. DELBLA.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1LLCX-ray3.00A2-326[»]
2ZQYX-ray2.60A/B/C/D1-326[»]
2ZQZX-ray2.50A/B/C/D/E/F1-326[»]
3VKUX-ray1.96A/B/C/D/E/F1-326[»]
3VKVX-ray2.70A/B/C/D/E/F1-326[»]
3VPFX-ray2.79A/B/C/D/E/F1-326[»]
ProteinModelPortaliP00343.
SMRiP00343. Positions 2-321.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi543734.LCABL_27160.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Phylogenomic databases

eggNOGiENOG4105C80. Bacteria.
COG0039. LUCA.

Enzyme and pathway databases

UniPathwayiUPA00554; UER00611.
BioCyciMetaCyc:MONOMER-8681.
SABIO-RKP00343.

Miscellaneous databases

EvolutionaryTraceiP00343.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
3.90.110.10. 1 hit.
HAMAPiMF_00488. Lactate_dehydrog. 1 hit.
InterProiIPR001557. L-lactate/malate_DH.
IPR011304. L-lactate_DH.
IPR018177. L-lactate_DH_AS.
IPR022383. Lactate/malate_DH_C.
IPR001236. Lactate/malate_DH_N.
IPR015955. Lactate_DH/Glyco_Ohase_4_C.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PANTHERiPTHR11540. PTHR11540. 1 hit.
PfamiPF02866. Ldh_1_C. 1 hit.
PF00056. Ldh_1_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000102. Lac_mal_DH. 1 hit.
PRINTSiPR00086. LLDHDRGNASE.
SUPFAMiSSF51735. SSF51735. 1 hit.
SSF56327. SSF56327. 1 hit.
TIGRFAMsiTIGR01771. L-LDH-NAD. 1 hit.
PROSITEiPS00064. L_LDH. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiLDH_LACCA
AccessioniPrimary (citable) accession number: P00343
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: January 23, 2007
Last modified: December 9, 2015
This is version 131 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Direct protein sequencing

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.