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P00343 (LDH_LACCA) Reviewed, UniProtKB/Swiss-Prot

Last modified May 29, 2013. Version 116. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
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to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
L-lactate dehydrogenase
Short name=L-LDH
EC=1.1.1.27
Gene names
Name:ldh
OrganismLactobacillus casei
Taxonomic identifier1582 [NCBI]
Taxonomic lineageBacteria › Firmicutes › Bacilli › Lactobacillales › Lactobacillaceae › Lactobacillus ›

Protein attributes

Sequence length326 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Catalytic activity

(S)-lactate + NAD+ = pyruvate + NADH. HAMAP-Rule MF_00488

Pathway

Fermentation; pyruvate fermentation to lactate; (S)-lactate from pyruvate: step 1/1. HAMAP-Rule MF_00488

Subunit structure

Homotetramer.

Subcellular location

Cytoplasm HAMAP-Rule MF_00488.

Sequence similarities

Belongs to the LDH/MDH superfamily. LDH family.

Ontologies

Keywords
   Biological processGlycolysis
   Cellular componentCytoplasm
   LigandNAD
   Molecular functionOxidoreductase
   PTMPhosphoprotein
   Technical term3D-structure
Direct protein sequencing
Gene Ontology (GO)
   Biological_processcellular carbohydrate metabolic process

Inferred from electronic annotation. Source: InterPro

glycolysis

Inferred from electronic annotation. Source: HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionL-lactate dehydrogenase activity

Inferred from electronic annotation. Source: HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed Ref.3
Chain2 – 326325L-lactate dehydrogenase HAMAP-Rule MF_00488
PRO_0000168347

Regions

Nucleotide binding18 – 4629NAD By similarity

Sites

Active site1811Proton acceptor By similarity
Binding site941Substrate By similarity
Binding site1261NAD or substrate By similarity
Binding site1571Substrate By similarity
Binding site2351Substrate By similarity

Amino acid modifications

Modified residue2261Phosphotyrosine By similarity

Experimental info

Sequence conflict261Y → F AA sequence Ref.3
Sequence conflict521I → T AA sequence Ref.3
Sequence conflict88 – 892QK → KQ AA sequence Ref.3
Sequence conflict1191G → L AA sequence Ref.3
Sequence conflict2701L → I AA sequence Ref.3
Sequence conflict2731I → L AA sequence Ref.3

Secondary structure

1 ...................................................... 326
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P00343 [UniParc].

Last modified January 23, 2007. Version 3.
Checksum: 934905641592AF8A

FASTA32635,531
        10         20         30         40         50         60 
MASITDKDHQ KVILVGDGAV GSSYAYAMVL QGIAQEIGIV DIFKDKTKGD AIDLSNALPF 

        70         80         90        100        110        120 
TSPKKIYSAE YSDAKDADLV VITAGAPQKP GETRLDLVNK NLKILKSIVD PIVDSGFNGI 

       130        140        150        160        170        180 
FLVAANPVDI LTYATWKLSG FPKNRVVGSG TSLDTARFRQ SIAEMVNVDA RSVHAYIMGE 

       190        200        210        220        230        240 
HGDTEFPVWS HANIGGVTIA EWVKAHPEIK EDKLVKMFED VRDAAYEIIK LKGATFYGIA 

       250        260        270        280        290        300 
TALARISKAI LNDENAVLPL SVYMDGQYGL NDIYIGTPAV INRNGIQNIL EIPLTDHEEE 

       310        320 
SMQKSASQLK KVLTDAFAKN DIETRQ

« Hide

References

[1]"Cloning and nucleotide sequence of the Lactobacillus casei lactate dehydrogenase gene."
Kim S.F., Baek S.J., Pack M.Y.
Appl. Environ. Microbiol. 57:2413-2417(1991) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 393 / DSM 20011 / JCM 1134 / NBRC 15883 / NCIMB 11970 / NCDO 161.
[2]Taguchi H., Ohta T.
Submitted (NOV-1991) to the PIR data bank
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 393 / DSM 20011 / JCM 1134 / NBRC 15883 / NCIMB 11970 / NCDO 161.
[3]"The complete primary structure of the allosteric L-lactate dehydrogenase from Lactobacillus casei."
Hensel R., Mayr U., Yang C.
Eur. J. Biochem. 134:503-511(1983) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 2-326.
[4]"Structure determination of the allosteric L-lactate dehydrogenase from Lactobacillus-casei at 3A resolution."
Buehner M., Hecht H.J.
Acta Crystallogr. A 40:32-34(1984)
Cited for: X-RAY CRYSTALLOGRAPHY (3.0 ANGSTROMS).
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		D12591 Genomic DNA. Translation: BAA02133.1.
M76708 Genomic DNA. Translation: AAA25245.2.
PIRDELBLA. A43944.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1LLCX-ray3.00A2-326[»]
2ZQYX-ray2.60A/B/C/D1-326[»]
2ZQZX-ray2.50A/B/C/D/E/F1-326[»]
3VKUX-ray1.96A/B/C/D/E/F1-326[»]
3VKVX-ray2.70A/B/C/D/E/F1-326[»]
3VPFX-ray2.79A/B/C/D/E/F1-326[»]
ProteinModelPortalP00343.
SMRP00343. Positions 2-321.
ModBaseSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Enzyme and pathway databases

BioCycMetaCyc:MONOMER-8681.
SABIO-RKP00343.
UniPathwayUPA00554; UER00611.

Family and domain databases

Gene3D3.40.50.720. 1 hit.
3.90.110.10. 1 hit.
HAMAPMF_00488. Lactate_dehydrog.
InterProIPR001557. L-lactate/malate_DH.
IPR011304. L-lactate_DH.
IPR018177. L-lactate_DH_AS.
IPR022383. Lactate/malate_DH_C.
IPR001236. Lactate/malate_DH_N.
IPR015955. Lactate_DH/Glyco_Ohase_4_C.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PANTHERPTHR11540. PTHR11540. 1 hit.
PTHR11540:SF3. PTHR11540:SF3. 1 hit.
PfamPF02866. Ldh_1_C. 1 hit.
PF00056. Ldh_1_N. 1 hit.
[Graphical view]
PIRSFPIRSF000102. Lac_mal_DH. 1 hit.
PRINTSPR00086. LLDHDRGNASE.
SUPFAMSSF56327. Lactate_DH/Glyco_hydro_4_C. 1 hit.
TIGRFAMsTIGR01771. L-LDH-NAD. 1 hit.
PROSITEPS00064. L_LDH. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceP00343.

Entry information

Entry nameLDH_LACCA
AccessionPrimary (citable) accession number: P00343
Entry history
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: January 23, 2007
Last modified: May 29, 2013
This is version 116 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

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