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Protein

L-lactate dehydrogenase C chain

Gene

Ldhc

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Possible role in sperm motility.By similarity

Catalytic activityi

(S)-lactate + NAD+ = pyruvate + NADH.

Pathwayi: pyruvate fermentation to lactate

This protein is involved in step 1 of the subpathway that synthesizes (S)-lactate from pyruvate.
Proteins known to be involved in this subpathway in this organism are:
  1. L-lactate dehydrogenase (Ldha), L-lactate dehydrogenase (Ldhb), L-lactate dehydrogenase (Ldhc), L-lactate dehydrogenase (Ldha), L-lactate dehydrogenase (Ldha), L-lactate dehydrogenase (Ldha), L-lactate dehydrogenase B chain (Ldhb), L-lactate dehydrogenase (Ldhc), L-lactate dehydrogenase (Ldhal6b), L-lactate dehydrogenase (Ldha), L-lactate dehydrogenase (Ldha), L-lactate dehydrogenase A chain (Ldha), L-lactate dehydrogenase (Ldhb), L-lactate dehydrogenase (Ldha), L-lactate dehydrogenase (Ldha), L-lactate dehydrogenase (Ldhc), L-lactate dehydrogenase (Ldha), L-lactate dehydrogenase (Ldha), L-lactate dehydrogenase C chain (Ldhc), L-lactate dehydrogenase (Ldhc)
This subpathway is part of the pathway pyruvate fermentation to lactate, which is itself part of Fermentation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes (S)-lactate from pyruvate, the pathway pyruvate fermentation to lactate and in Fermentation.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei99NADBy similarity1
Binding sitei106SubstrateBy similarity1
Binding sitei138NAD or substrateBy similarity1
Binding sitei169SubstrateBy similarity1
Active sitei193Proton acceptorBy similarity1
Binding sitei248SubstrateBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi29 – 57NADBy similarityAdd BLAST29

GO - Molecular functioni

  • L-lactate dehydrogenase activity Source: MGI

GO - Biological processi

  • ATP biosynthetic process Source: MGI
  • carbohydrate metabolic process Source: InterPro
  • flagellated sperm motility Source: MGI
  • lactate biosynthetic process from pyruvate Source: MGI
  • lactate oxidation Source: MGI
  • pyruvate metabolic process Source: MGI

Keywordsi

Molecular functionOxidoreductase
LigandNAD

Enzyme and pathway databases

ReactomeiR-MMU-70268 Pyruvate metabolism
UniPathwayiUPA00554; UER00611

Names & Taxonomyi

Protein namesi
Recommended name:
L-lactate dehydrogenase C chain (EC:1.1.1.27)
Short name:
LDH-C
Alternative name(s):
LDH testis subunit
LDH-X
Gene namesi
Name:Ldhc
Synonyms:Ldh-3, Ldh3
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 7

Organism-specific databases

MGIiMGI:96764 Ldhc

Subcellular locationi

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved1 Publication
ChainiPRO_00001684802 – 332L-lactate dehydrogenase C chainAdd BLAST331

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2Blocked amino end (Ser)1

Proteomic databases

PaxDbiP00342
PeptideAtlasiP00342
PRIDEiP00342

2D gel databases

REPRODUCTION-2DPAGEiP00342

PTM databases

iPTMnetiP00342
PhosphoSitePlusiP00342
SwissPalmiP00342

Expressioni

Tissue specificityi

Expressed within the midpiece of sperm tail (at protein level).1 Publication

Gene expression databases

BgeeiENSMUSG00000030851
CleanExiMM_LDHC
ExpressionAtlasiP00342 baseline and differential
GenevisibleiP00342 MM

Interactioni

Subunit structurei

Homotetramer. Interacts with RABL2/RABL2A; binds preferentially to GTP-bound RABL2.1 Publication

Protein-protein interaction databases

BioGridi201131, 2 interactors
IntActiP00342, 2 interactors
MINTiP00342
STRINGi10090.ENSMUSP00000014545

Chemistry databases

BindingDBiP00342

Structurei

Secondary structure

1332
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi4 – 8Combined sources5
Beta strandi9 – 11Combined sources3
Beta strandi22 – 26Combined sources5
Helixi30 – 40Combined sources11
Turni41 – 43Combined sources3
Beta strandi46 – 51Combined sources6
Helixi55 – 67Combined sources13
Turni68 – 71Combined sources4
Beta strandi76 – 82Combined sources7
Helixi83 – 86Combined sources4
Beta strandi89 – 94Combined sources6
Turni102 – 104Combined sources3
Helixi111 – 120Combined sources10
Turni121 – 123Combined sources3
Helixi124 – 127Combined sources4
Beta strandi132 – 135Combined sources4
Beta strandi137 – 139Combined sources3
Helixi140 – 151Combined sources12
Turni155 – 157Combined sources3
Beta strandi158 – 160Combined sources3
Helixi164 – 178Combined sources15
Beta strandi189 – 191Combined sources3
Beta strandi193 – 195Combined sources3
Beta strandi197 – 199Combined sources3
Helixi201 – 203Combined sources3
Beta strandi205 – 207Combined sources3
Beta strandi209 – 214Combined sources6
Turni215 – 217Combined sources3
Beta strandi221 – 226Combined sources6
Helixi228 – 245Combined sources18
Helixi250 – 264Combined sources15
Beta strandi269 – 276Combined sources8
Beta strandi288 – 296Combined sources9
Beta strandi299 – 304Combined sources6
Helixi310 – 326Combined sources17

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2LDXX-ray2.96A/B/C/D2-332[»]
ProteinModelPortaliP00342
SMRiP00342
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP00342

Family & Domainsi

Sequence similaritiesi

Belongs to the LDH/MDH superfamily. LDH family.Curated

Phylogenomic databases

eggNOGiKOG1495 Eukaryota
COG0039 LUCA
GeneTreeiENSGT00550000074541
HOGENOMiHOG000213793
HOVERGENiHBG000462
InParanoidiP00342
KOiK00016
OMAiDQWKNIH
OrthoDBiEOG091G0HME
PhylomeDBiP00342
TreeFamiTF314963

Family and domain databases

Gene3Di3.90.110.10, 1 hit
HAMAPiMF_00488 Lactate_dehydrog, 1 hit
InterProiView protein in InterPro
IPR001557 L-lactate/malate_DH
IPR011304 L-lactate_DH
IPR018177 L-lactate_DH_AS
IPR022383 Lactate/malate_DH_C
IPR001236 Lactate/malate_DH_N
IPR015955 Lactate_DH/Glyco_Ohase_4_C
IPR036291 NAD(P)-bd_dom_sf
PfamiView protein in Pfam
PF02866 Ldh_1_C, 1 hit
PF00056 Ldh_1_N, 1 hit
PIRSFiPIRSF000102 Lac_mal_DH, 1 hit
PRINTSiPR00086 LLDHDRGNASE
SUPFAMiSSF51735 SSF51735, 1 hit
SSF56327 SSF56327, 1 hit
TIGRFAMsiTIGR01771 L-LDH-NAD, 1 hit
PROSITEiView protein in PROSITE
PS00064 L_LDH, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P00342-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSTVKEQLIQ NLVPEDKLSR CKITVVGVGN VGMACAISIL LKGLADELAL
60 70 80 90 100
VDADTNKLRG EALDLLHGSL FLSTPKIVFG KDYNVSANSK LVIITAGARM
110 120 130 140 150
VSGETRLDLL QRNVAIMKAI VPGIVQNSPD CKIIIVTNPV DILTYVVWKI
160 170 180 190 200
SGFPVGRVIG SGCNLDSARF RYLIGEKLGV NPTSCHGWVL GEHGDSSVPI
210 220 230 240 250
WSGVNVAGVT LKSLNPAIGT DSDKEHWKNV HKQVVEGGYE VLNMKGYTSW
260 270 280 290 300
AIGLSVTDLA RSILKNLKRV HPVTTLVKGF HGIKEEVFLS IPCVLGQSGI
310 320 330
TDFVKVNMTA EEEGLLKKSA DTLWNMQKDL QL
Length:332
Mass (Da):35,912
Last modified:January 23, 2007 - v2
Checksum:i367A7EA8B2A6D86A
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti7Q → E AA sequence (PubMed:6343385).Curated1
Sequence conflicti15Missing AA sequence (PubMed:6343385).Curated1
Sequence conflicti30N → D AA sequence (PubMed:6343385).Curated1
Sequence conflicti56N → D AA sequence (PubMed:6343385).Curated1
Sequence conflicti104E → Q AA sequence (PubMed:6343385).Curated1
Sequence conflicti124 – 125IV → VI AA sequence (PubMed:6343385).Curated2
Sequence conflicti135I → V AA sequence (PubMed:6343385).Curated1
Sequence conflicti223 – 225DKE → NKQ AA sequence (PubMed:6343385).Curated3
Sequence conflicti243N → D AA sequence (PubMed:6343385).Curated1
Sequence conflicti297Q → E AA sequence (PubMed:6343385).Curated1
Sequence conflicti329 – 331DLQ → NLE AA sequence (PubMed:6343385).Curated3

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X04752 mRNA Translation: CAA28449.1
M17587 mRNA Translation: AAA39425.1
BC049602 mRNA Translation: AAH49602.1
M12781 mRNA Translation: AAA88315.1
CCDSiCCDS21290.1
PIRiA26824 DEMSLC
RefSeqiNP_038608.1, NM_013580.4
XP_006540742.1, XM_006540679.1
XP_006540743.1, XM_006540680.1
XP_006540744.1, XM_006540681.1
UniGeneiMm.16563

Genome annotation databases

EnsembliENSMUST00000014545; ENSMUSP00000014545; ENSMUSG00000030851
GeneIDi16833
KEGGimmu:16833
UCSCiuc009gzp.1 mouse

Similar proteinsi

Entry informationi

Entry nameiLDHC_MOUSE
AccessioniPrimary (citable) accession number: P00342
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: January 23, 2007
Last modified: May 23, 2018
This is version 174 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

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