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Protein

L-lactate dehydrogenase C chain

Gene

Ldhc

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Possible role in sperm motility.By similarity

Catalytic activityi

(S)-lactate + NAD+ = pyruvate + NADH.

Pathwayi: pyruvate fermentation to lactate

This protein is involved in step 1 of the subpathway that synthesizes (S)-lactate from pyruvate.
Proteins known to be involved in this subpathway in this organism are:
  1. L-lactate dehydrogenase (Ldha), L-lactate dehydrogenase (Ldhb), L-lactate dehydrogenase (Ldhc), L-lactate dehydrogenase (Ldha), L-lactate dehydrogenase (Ldha), L-lactate dehydrogenase (Ldha), L-lactate dehydrogenase B chain (Ldhb), L-lactate dehydrogenase (Ldhc), L-lactate dehydrogenase (Ldhal6b), L-lactate dehydrogenase (Ldha), L-lactate dehydrogenase (Ldha), L-lactate dehydrogenase A chain (Ldha), L-lactate dehydrogenase (Ldhb), L-lactate dehydrogenase (Ldha), L-lactate dehydrogenase (Ldha), L-lactate dehydrogenase (Ldhc), L-lactate dehydrogenase (Ldha), L-lactate dehydrogenase (Ldha), L-lactate dehydrogenase C chain (Ldhc), L-lactate dehydrogenase (Ldhc)
This subpathway is part of the pathway pyruvate fermentation to lactate, which is itself part of Fermentation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes (S)-lactate from pyruvate, the pathway pyruvate fermentation to lactate and in Fermentation.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei99NADBy similarity1
Binding sitei106SubstrateBy similarity1
Binding sitei138NAD or substrateBy similarity1
Binding sitei169SubstrateBy similarity1
Active sitei193Proton acceptorBy similarity1
Binding sitei248SubstrateBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi29 – 57NADBy similarityAdd BLAST29

GO - Molecular functioni

  • L-lactate dehydrogenase activity Source: MGI

GO - Biological processi

  • ATP biosynthetic process Source: MGI
  • carbohydrate metabolic process Source: InterPro
  • flagellated sperm motility Source: MGI
  • lactate biosynthetic process from pyruvate Source: MGI
  • lactate oxidation Source: MGI
  • pyruvate metabolic process Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Ligandi

NAD

Enzyme and pathway databases

ReactomeiR-MMU-70268. Pyruvate metabolism.
UniPathwayiUPA00554; UER00611.

Names & Taxonomyi

Protein namesi
Recommended name:
L-lactate dehydrogenase C chain (EC:1.1.1.27)
Short name:
LDH-C
Alternative name(s):
LDH testis subunit
LDH-X
Gene namesi
Name:Ldhc
Synonyms:Ldh-3, Ldh3
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 7

Organism-specific databases

MGIiMGI:96764. Ldhc.

Subcellular locationi

GO - Cellular componenti

  • cilium Source: MGI
  • cytoplasm Source: MGI
  • extracellular exosome Source: MGI
  • motile cilium Source: MGI
  • nucleus Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved1 Publication
ChainiPRO_00001684802 – 332L-lactate dehydrogenase C chainAdd BLAST331

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2Blocked amino end (Ser)1

Proteomic databases

PaxDbiP00342.
PeptideAtlasiP00342.
PRIDEiP00342.

2D gel databases

REPRODUCTION-2DPAGEP00342.

PTM databases

iPTMnetiP00342.
PhosphoSitePlusiP00342.

Expressioni

Tissue specificityi

Expressed within the midpiece of sperm tail (at protein level).1 Publication

Gene expression databases

BgeeiENSMUSG00000030851.
CleanExiMM_LDHC.
ExpressionAtlasiP00342. baseline and differential.
GenevisibleiP00342. MM.

Interactioni

Subunit structurei

Homotetramer. Interacts with RABL2/RABL2A; binds preferentially to GTP-bound RABL2.1 Publication

Protein-protein interaction databases

BioGridi201131. 1 interactor.
IntActiP00342. 2 interactors.
MINTiMINT-6611882.
STRINGi10090.ENSMUSP00000014545.

Structurei

Secondary structure

1332
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi4 – 8Combined sources5
Beta strandi9 – 11Combined sources3
Beta strandi22 – 26Combined sources5
Helixi30 – 40Combined sources11
Turni41 – 43Combined sources3
Beta strandi46 – 51Combined sources6
Helixi55 – 67Combined sources13
Turni68 – 71Combined sources4
Beta strandi76 – 82Combined sources7
Helixi83 – 86Combined sources4
Beta strandi89 – 94Combined sources6
Turni102 – 104Combined sources3
Helixi111 – 120Combined sources10
Turni121 – 123Combined sources3
Helixi124 – 127Combined sources4
Beta strandi132 – 135Combined sources4
Beta strandi137 – 139Combined sources3
Helixi140 – 151Combined sources12
Turni155 – 157Combined sources3
Beta strandi158 – 160Combined sources3
Helixi164 – 178Combined sources15
Beta strandi189 – 191Combined sources3
Beta strandi193 – 195Combined sources3
Beta strandi197 – 199Combined sources3
Helixi201 – 203Combined sources3
Beta strandi205 – 207Combined sources3
Beta strandi209 – 214Combined sources6
Turni215 – 217Combined sources3
Beta strandi221 – 226Combined sources6
Helixi228 – 245Combined sources18
Helixi250 – 264Combined sources15
Beta strandi269 – 276Combined sources8
Beta strandi288 – 296Combined sources9
Beta strandi299 – 304Combined sources6
Helixi310 – 326Combined sources17

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2LDXX-ray2.96A/B/C/D2-332[»]
ProteinModelPortaliP00342.
SMRiP00342.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP00342.

Family & Domainsi

Sequence similaritiesi

Belongs to the LDH/MDH superfamily. LDH family.Curated

Phylogenomic databases

eggNOGiKOG1495. Eukaryota.
COG0039. LUCA.
GeneTreeiENSGT00550000074541.
HOGENOMiHOG000213793.
HOVERGENiHBG000462.
InParanoidiP00342.
KOiK00016.
OMAiXAYEIIK.
OrthoDBiEOG091G0HME.
PhylomeDBiP00342.
TreeFamiTF314963.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
3.90.110.10. 1 hit.
HAMAPiMF_00488. Lactate_dehydrog. 1 hit.
InterProiIPR001557. L-lactate/malate_DH.
IPR011304. L-lactate_DH.
IPR018177. L-lactate_DH_AS.
IPR022383. Lactate/malate_DH_C.
IPR001236. Lactate/malate_DH_N.
IPR015955. Lactate_DH/Glyco_Ohase_4_C.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PANTHERiPTHR11540. PTHR11540. 1 hit.
PfamiPF02866. Ldh_1_C. 1 hit.
PF00056. Ldh_1_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000102. Lac_mal_DH. 1 hit.
PRINTSiPR00086. LLDHDRGNASE.
SUPFAMiSSF51735. SSF51735. 1 hit.
SSF56327. SSF56327. 1 hit.
TIGRFAMsiTIGR01771. L-LDH-NAD. 1 hit.
PROSITEiPS00064. L_LDH. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P00342-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSTVKEQLIQ NLVPEDKLSR CKITVVGVGN VGMACAISIL LKGLADELAL
60 70 80 90 100
VDADTNKLRG EALDLLHGSL FLSTPKIVFG KDYNVSANSK LVIITAGARM
110 120 130 140 150
VSGETRLDLL QRNVAIMKAI VPGIVQNSPD CKIIIVTNPV DILTYVVWKI
160 170 180 190 200
SGFPVGRVIG SGCNLDSARF RYLIGEKLGV NPTSCHGWVL GEHGDSSVPI
210 220 230 240 250
WSGVNVAGVT LKSLNPAIGT DSDKEHWKNV HKQVVEGGYE VLNMKGYTSW
260 270 280 290 300
AIGLSVTDLA RSILKNLKRV HPVTTLVKGF HGIKEEVFLS IPCVLGQSGI
310 320 330
TDFVKVNMTA EEEGLLKKSA DTLWNMQKDL QL
Length:332
Mass (Da):35,912
Last modified:January 23, 2007 - v2
Checksum:i367A7EA8B2A6D86A
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti7Q → E AA sequence (PubMed:6343385).Curated1
Sequence conflicti15Missing AA sequence (PubMed:6343385).Curated1
Sequence conflicti30N → D AA sequence (PubMed:6343385).Curated1
Sequence conflicti56N → D AA sequence (PubMed:6343385).Curated1
Sequence conflicti104E → Q AA sequence (PubMed:6343385).Curated1
Sequence conflicti124 – 125IV → VI AA sequence (PubMed:6343385).Curated2
Sequence conflicti135I → V AA sequence (PubMed:6343385).Curated1
Sequence conflicti223 – 225DKE → NKQ AA sequence (PubMed:6343385).Curated3
Sequence conflicti243N → D AA sequence (PubMed:6343385).Curated1
Sequence conflicti297Q → E AA sequence (PubMed:6343385).Curated1
Sequence conflicti329 – 331DLQ → NLE AA sequence (PubMed:6343385).Curated3

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X04752 mRNA. Translation: CAA28449.1.
M17587 mRNA. Translation: AAA39425.1.
BC049602 mRNA. Translation: AAH49602.1.
M12781 mRNA. Translation: AAA88315.1.
CCDSiCCDS21290.1.
PIRiA26824. DEMSLC.
RefSeqiNP_038608.1. NM_013580.4.
XP_006540742.1. XM_006540679.1.
XP_006540743.1. XM_006540680.1.
XP_006540744.1. XM_006540681.1.
UniGeneiMm.16563.

Genome annotation databases

EnsembliENSMUST00000014545; ENSMUSP00000014545; ENSMUSG00000030851.
GeneIDi16833.
KEGGimmu:16833.
UCSCiuc009gzp.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X04752 mRNA. Translation: CAA28449.1.
M17587 mRNA. Translation: AAA39425.1.
BC049602 mRNA. Translation: AAH49602.1.
M12781 mRNA. Translation: AAA88315.1.
CCDSiCCDS21290.1.
PIRiA26824. DEMSLC.
RefSeqiNP_038608.1. NM_013580.4.
XP_006540742.1. XM_006540679.1.
XP_006540743.1. XM_006540680.1.
XP_006540744.1. XM_006540681.1.
UniGeneiMm.16563.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2LDXX-ray2.96A/B/C/D2-332[»]
ProteinModelPortaliP00342.
SMRiP00342.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi201131. 1 interactor.
IntActiP00342. 2 interactors.
MINTiMINT-6611882.
STRINGi10090.ENSMUSP00000014545.

PTM databases

iPTMnetiP00342.
PhosphoSitePlusiP00342.

2D gel databases

REPRODUCTION-2DPAGEP00342.

Proteomic databases

PaxDbiP00342.
PeptideAtlasiP00342.
PRIDEiP00342.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000014545; ENSMUSP00000014545; ENSMUSG00000030851.
GeneIDi16833.
KEGGimmu:16833.
UCSCiuc009gzp.1. mouse.

Organism-specific databases

CTDi3948.
MGIiMGI:96764. Ldhc.

Phylogenomic databases

eggNOGiKOG1495. Eukaryota.
COG0039. LUCA.
GeneTreeiENSGT00550000074541.
HOGENOMiHOG000213793.
HOVERGENiHBG000462.
InParanoidiP00342.
KOiK00016.
OMAiXAYEIIK.
OrthoDBiEOG091G0HME.
PhylomeDBiP00342.
TreeFamiTF314963.

Enzyme and pathway databases

UniPathwayiUPA00554; UER00611.
ReactomeiR-MMU-70268. Pyruvate metabolism.

Miscellaneous databases

ChiTaRSiLdhc. mouse.
EvolutionaryTraceiP00342.
PROiP00342.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000030851.
CleanExiMM_LDHC.
ExpressionAtlasiP00342. baseline and differential.
GenevisibleiP00342. MM.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
3.90.110.10. 1 hit.
HAMAPiMF_00488. Lactate_dehydrog. 1 hit.
InterProiIPR001557. L-lactate/malate_DH.
IPR011304. L-lactate_DH.
IPR018177. L-lactate_DH_AS.
IPR022383. Lactate/malate_DH_C.
IPR001236. Lactate/malate_DH_N.
IPR015955. Lactate_DH/Glyco_Ohase_4_C.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PANTHERiPTHR11540. PTHR11540. 1 hit.
PfamiPF02866. Ldh_1_C. 1 hit.
PF00056. Ldh_1_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000102. Lac_mal_DH. 1 hit.
PRINTSiPR00086. LLDHDRGNASE.
SUPFAMiSSF51735. SSF51735. 1 hit.
SSF56327. SSF56327. 1 hit.
TIGRFAMsiTIGR01771. L-LDH-NAD. 1 hit.
PROSITEiPS00064. L_LDH. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiLDHC_MOUSE
AccessioniPrimary (citable) accession number: P00342
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: January 23, 2007
Last modified: November 30, 2016
This is version 166 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.