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Protein

L-lactate dehydrogenase A chain

Gene

LDHA

Organism
Gallus gallus (Chicken)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

(S)-lactate + NAD+ = pyruvate + NADH.

Pathwayi: pyruvate fermentation to lactate

This protein is involved in step 1 of the subpathway that synthesizes (S)-lactate from pyruvate.
Proteins known to be involved in this subpathway in this organism are:
  1. L-lactate dehydrogenase A chain (LDHA), L-lactate dehydrogenase (LDHA), L-lactate dehydrogenase B chain (LDHB)
This subpathway is part of the pathway pyruvate fermentation to lactate, which is itself part of Fermentation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes (S)-lactate from pyruvate, the pathway pyruvate fermentation to lactate and in Fermentation.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei99 – 991NADBy similarity
Binding sitei106 – 1061SubstrateBy similarity
Binding sitei138 – 1381NAD or substrateBy similarity
Binding sitei169 – 1691SubstrateBy similarity
Active sitei193 – 1931Proton acceptorBy similarity
Binding sitei248 – 2481SubstrateBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi29 – 5729NADBy similarityAdd
BLAST

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Ligandi

NAD

Enzyme and pathway databases

ReactomeiR-GGA-373920. Pyruvate metabolism.
SABIO-RKP00340.
UniPathwayiUPA00554; UER00611.

Names & Taxonomyi

Protein namesi
Recommended name:
L-lactate dehydrogenase A chain (EC:1.1.1.27)
Short name:
LDH-A
Gene namesi
Name:LDHA
OrganismiGallus gallus (Chicken)
Taxonomic identifieri9031 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiArchelosauriaArchosauriaDinosauriaSaurischiaTheropodaCoelurosauriaAvesNeognathaeGalloanseraeGalliformesPhasianidaePhasianinaeGallus
Proteomesi
  • UP000000539 Componenti: Unplaced

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemoved1 Publication
Chaini2 – 332331L-lactate dehydrogenase A chainPRO_0000168420Add
BLAST

Proteomic databases

PaxDbiP00340.
PRIDEiP00340.

PTM databases

iPTMnetiP00340.

Interactioni

Subunit structurei

Homotetramer.

Protein-protein interaction databases

BioGridi676481. 2 interactions.
IntActiP00340. 1 interaction.
STRINGi9031.ENSGALP00000010167.

Structurei

3D structure databases

ProteinModelPortaliP00340.
SMRiP00340. Positions 2-332.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the LDH/MDH superfamily. LDH family.Curated

Phylogenomic databases

eggNOGiKOG1495. Eukaryota.
COG0039. LUCA.
HOGENOMiHOG000213793.
HOVERGENiHBG000462.
InParanoidiP00340.
KOiK00016.
PhylomeDBiP00340.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
3.90.110.10. 1 hit.
HAMAPiMF_00488. Lactate_dehydrog.
InterProiIPR001557. L-lactate/malate_DH.
IPR011304. L-lactate_DH.
IPR018177. L-lactate_DH_AS.
IPR022383. Lactate/malate_DH_C.
IPR001236. Lactate/malate_DH_N.
IPR015955. Lactate_DH/Glyco_Ohase_4_C.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PANTHERiPTHR11540. PTHR11540. 1 hit.
PfamiPF02866. Ldh_1_C. 1 hit.
PF00056. Ldh_1_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000102. Lac_mal_DH. 1 hit.
PRINTSiPR00086. LLDHDRGNASE.
SUPFAMiSSF51735. SSF51735. 1 hit.
SSF56327. SSF56327. 1 hit.
TIGRFAMsiTIGR01771. L-LDH-NAD. 1 hit.
PROSITEiPS00064. L_LDH. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P00340-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSLKDHLIHN VHKEEHAHAH NKISVVGVGA VGMACAISIL MKDLADELTL
60 70 80 90 100
VDVVEDKLKG EMLDLQHGSL FLKTPKIISG KDYSVTAHSK LVIVTAGARQ
110 120 130 140 150
QEGESRLNLV QRNVNIFKFI IPNVVKYSPD CKLLIVSNPV DILTYVAWKI
160 170 180 190 200
SGFPKHRVIG SGCNLDSARF RHLMGERLGI HPLSCHGWIV GEHGDSSVPV
210 220 230 240 250
WSGVNVAGVS LKALHPDMGT DADKEHWKEV HKQVVDSAYE VIKLKGYTSW
260 270 280 290 300
AIGLSVADLA ETIMKNLRRV HPISTAVKGM HGIKDDVFLS VPCVLGSSGI
310 320 330
TDVVKMILKP DEEEKIKKSA DTLWGIQKEL QF
Length:332
Mass (Da):36,514
Last modified:January 23, 2007 - v3
Checksum:i5ED8279CFAD7B62B
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti63 – 631L → M AA sequence (Ref. 2) Curated
Sequence conflicti78 – 781I → T AA sequence (Ref. 2) Curated
Sequence conflicti192 – 1921E → Q AA sequence (Ref. 2) Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X53828 mRNA. Translation: CAA37824.1.
PIRiA00349. DECHLM.
S12151.
RefSeqiNP_990615.1. NM_205284.1.
UniGeneiGga.4398.

Genome annotation databases

GeneIDi396221.
KEGGigga:396221.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X53828 mRNA. Translation: CAA37824.1.
PIRiA00349. DECHLM.
S12151.
RefSeqiNP_990615.1. NM_205284.1.
UniGeneiGga.4398.

3D structure databases

ProteinModelPortaliP00340.
SMRiP00340. Positions 2-332.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi676481. 2 interactions.
IntActiP00340. 1 interaction.
STRINGi9031.ENSGALP00000010167.

PTM databases

iPTMnetiP00340.

Proteomic databases

PaxDbiP00340.
PRIDEiP00340.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi396221.
KEGGigga:396221.

Organism-specific databases

CTDi3939.

Phylogenomic databases

eggNOGiKOG1495. Eukaryota.
COG0039. LUCA.
HOGENOMiHOG000213793.
HOVERGENiHBG000462.
InParanoidiP00340.
KOiK00016.
PhylomeDBiP00340.

Enzyme and pathway databases

UniPathwayiUPA00554; UER00611.
ReactomeiR-GGA-373920. Pyruvate metabolism.
SABIO-RKP00340.

Miscellaneous databases

PROiP00340.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
3.90.110.10. 1 hit.
HAMAPiMF_00488. Lactate_dehydrog.
InterProiIPR001557. L-lactate/malate_DH.
IPR011304. L-lactate_DH.
IPR018177. L-lactate_DH_AS.
IPR022383. Lactate/malate_DH_C.
IPR001236. Lactate/malate_DH_N.
IPR015955. Lactate_DH/Glyco_Ohase_4_C.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PANTHERiPTHR11540. PTHR11540. 1 hit.
PfamiPF02866. Ldh_1_C. 1 hit.
PF00056. Ldh_1_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000102. Lac_mal_DH. 1 hit.
PRINTSiPR00086. LLDHDRGNASE.
SUPFAMiSSF51735. SSF51735. 1 hit.
SSF56327. SSF56327. 1 hit.
TIGRFAMsiTIGR01771. L-LDH-NAD. 1 hit.
PROSITEiPS00064. L_LDH. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Nucleotide and deduced amino acid sequences of chicken lactate dehydrogenase-A."
    Hirota Y., Katsumata A., Takeya T.
    Nucleic Acids Res. 18:6432-6432(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. Torff H.-J., Becker D., Schwarzwalder J.
    (In) Sund H. (eds.); Pyridine nucleotide dependent dehydrogenases, pp.31-42, Walter de Gruyter, Berlin (1977)
    Cited for: PROTEIN SEQUENCE OF 2-332.

Entry informationi

Entry nameiLDHA_CHICK
AccessioniPrimary (citable) accession number: P00340
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: January 23, 2007
Last modified: June 8, 2016
This is version 127 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.