Reviewed, UniProtKB/Swiss-Prot P00340 (LDHA_CHICK)

Names and origin

Protein names

Recommended name:
    L-lactate dehydrogenase A chain
      Short name=LDH-A
    EC=1.1.1.27

Gene names

Name:

LDHA

Organism

Gallus gallus (Chicken)

Taxonomic identifier

9031 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Archosauria › Dinosauria › Saurischia › Theropoda › Coelurosauria › Aves › Neognathae › Galliformes › Phasianidae › Phasianinae › Gallus

Protein attributes

Sequence length	332 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Catalytic activity	(S)-lactate + NAD+ = pyruvate + NADH.
Pathway	Fermentation; pyruvate fermentation to lactate; (S)-lactate from pyruvate: step 1/1.
Subunit structure	Homotetramer.
Subcellular location	Cytoplasm.
Sequence similarities	Belongs to the LDH/MDH superfamily. LDH family.

Ontologies

Keywords
Biological process	Glycolysis
Cellular component	Cytoplasm
Ligand	NAD
Molecular function	Oxidoreductase
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	anaerobic glycolysis Inferred from electronic annotation. Source: InterPro oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	L-lactate dehydrogenase activity Inferred from electronic annotation. Source: EC binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed Ref.2
	Chain	2 – 332	331	L-lactate dehydrogenase A chain		PRO_0000168420
Regions
	Nucleotide binding	29 – 57	29	NAD By similarity
Sites
	Active site	193	1	Proton acceptor By similarity
	Binding site	99	1	NAD By similarity
	Binding site	106	1	Substrate By similarity
	Binding site	138	1	NAD or substrate By similarity
	Binding site	169	1	Substrate By similarity
	Binding site	248	1	Substrate By similarity
Experimental info
	Sequence conflict	63	1	L → M AA sequence Ref.2
	Sequence conflict	78	1	I → T AA sequence Ref.2
	Sequence conflict	192	1	E → Q AA sequence Ref.2

Sequences

	Sequence		Length	Mass (Da)
	P00340-1 [UniParc]. Last modified January 23, 2007. Version 3. Checksum: 5ED8279CFAD7B62B	FASTA	332	36,514
	10 20 30 40 50 60 MSLKDHLIHN VHKEEHAHAH NKISVVGVGA VGMACAISIL MKDLADELTL VDVVEDKLKG 70 80 90 100 110 120 EMLDLQHGSL FLKTPKIISG KDYSVTAHSK LVIVTAGARQ QEGESRLNLV QRNVNIFKFI 130 140 150 160 170 180 IPNVVKYSPD CKLLIVSNPV DILTYVAWKI SGFPKHRVIG SGCNLDSARF RHLMGERLGI 190 200 210 220 230 240 HPLSCHGWIV GEHGDSSVPV WSGVNVAGVS LKALHPDMGT DADKEHWKEV HKQVVDSAYE 250 260 270 280 290 300 VIKLKGYTSW AIGLSVADLA ETIMKNLRRV HPISTAVKGM HGIKDDVFLS VPCVLGSSGI 310 320 330 TDVVKMILKP DEEEKIKKSA DTLWGIQKEL QF

References

[1]	"Nucleotide and deduced amino acid sequences of chicken lactate dehydrogenase-A." Hirota Y., Katsumata A., Takeya T. Nucleic Acids Res. 18:6432-6432(1990) [PubMed: 2243792] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	Torff H.-J., Becker D., Schwarzwalder J. (In) Sund H. (eds.); Pyridine nucleotide dependent dehydrogenases, pp.31-42, Walter de Gruyter, Berlin (1977) Cited for: PROTEIN SEQUENCE OF 2-332.

Cross-references

Sequence databases
EMBLGenBankDDBJ	X53828 mRNA. Translation: CAA37824.1.
IPI	IPI00584727.
PIR	DECHLM. A00349. S12151.
RefSeq	NP_990615.1.
UniGene	Gga.4398
3D structure databases
HSSP	HSSP built from PDB template 1I10 based on UniProtKB P00338.
SMR	P00340. Positions 2-332.
ModBase	Search...
Proteomic databases
PRIDE	P00340.
Genome annotation databases
Ensembl	ENSGALG00000006300. Gallus gallus. [Contig view]
GeneID	396221.
KEGG	gga:396221.
Phylogenomic databases
HOGENOM	P00340.
HOVERGEN	P00340.
Enzyme and pathway databases
BRENDA	1.1.1.27. 4.
Family and domain databases
InterPro	IPR001557. L-lactate/malate_DH. IPR011304. L-lactate_DH. IPR018177. L-lactate_DH_AS. IPR001236. Lactate/malate_DH. IPR015955. Lactate_DH/Glyco_Ohase_4_C. IPR016040. NAD(P)-bd_dom. [Graphical view]
Gene3D	G3DSA:3.90.110.10. lact_mal_DH. 1 hit. G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
Pfam	PF02866. Ldh_1_C. 1 hit. PF00056. Ldh_1_N. 1 hit. [Graphical view]
PIRSF	PIRSF000102. Lac_mal_DH. 1 hit.
PRINTS	PR00086. LLDHDRGNASE.
TIGRFAMs	TIGR01771. L-LDH-NAD. 1 hit.
PROSITE	PS00064. L_LDH. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

LDHA_CHICK

Accession

Primary (citable) accession number: P00340

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 21, 1986
Last sequence update:	January 23, 2007
Last modified:	June 16, 2009
This is version 74 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)