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Protein

L-lactate dehydrogenase A chain

Gene

LDHA

Organism
Sus scrofa (Pig)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

(S)-lactate + NAD+ = pyruvate + NADH.

Pathwayi: pyruvate fermentation to lactate

This protein is involved in step 1 of the subpathway that synthesizes (S)-lactate from pyruvate.
Proteins known to be involved in this subpathway in this organism are:
  1. L-lactate dehydrogenase, L-lactate dehydrogenase (LDHAL6B), L-lactate dehydrogenase, L-lactate dehydrogenase (LDHB), L-lactate dehydrogenase A chain (LDHA), L-lactate dehydrogenase C chain (LDHC), L-lactate dehydrogenase B chain (LDHB), L-lactate dehydrogenase (LDHC), L-lactate dehydrogenase (LDHAL6B), L-lactate dehydrogenase, L-lactate dehydrogenase
This subpathway is part of the pathway pyruvate fermentation to lactate, which is itself part of Fermentation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes (S)-lactate from pyruvate, the pathway pyruvate fermentation to lactate and in Fermentation.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei99 – 991NAD1 Publication
Binding sitei106 – 1061Substrate
Binding sitei138 – 1381NAD or substrate1 Publication
Binding sitei169 – 1691Substrate
Active sitei193 – 1931Proton acceptor
Binding sitei248 – 2481Substrate

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi29 – 5729NAD1 PublicationAdd
BLAST

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Ligandi

NAD

Enzyme and pathway databases

ReactomeiR-SSC-70268. Pyruvate metabolism.
UniPathwayiUPA00554; UER00611.

Names & Taxonomyi

Protein namesi
Recommended name:
L-lactate dehydrogenase A chain (EC:1.1.1.27)
Short name:
LDH-A
Alternative name(s):
LDH muscle subunit
Short name:
LDH-M
Gene namesi
Name:LDHA
OrganismiSus scrofa (Pig)
Taxonomic identifieri9823 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaSuinaSuidaeSus
Proteomesi
  • UP000008227 Componenti: Chromosome 2

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemoved1 Publication
Chaini2 – 332331L-lactate dehydrogenase A chainPRO_0000168416Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylalanine1 Publication
Modified residuei5 – 51N6-acetyllysine; alternateBy similarity
Modified residuei5 – 51N6-succinyllysine; alternateBy similarity
Modified residuei14 – 141N6-acetyllysineBy similarity
Modified residuei57 – 571N6-acetyllysineBy similarity
Modified residuei81 – 811N6-acetyllysineBy similarity
Modified residuei118 – 1181N6-acetyllysine; alternateBy similarity
Modified residuei118 – 1181N6-succinyllysine; alternateBy similarity
Modified residuei126 – 1261N6-acetyllysineBy similarity
Modified residuei224 – 2241N6-acetyllysineBy similarity
Modified residuei232 – 2321N6-acetyllysineBy similarity
Modified residuei239 – 2391PhosphotyrosineBy similarity
Modified residuei243 – 2431N6-acetyllysineBy similarity
Modified residuei309 – 3091PhosphothreonineBy similarity
Modified residuei318 – 3181N6-acetyllysine; alternateBy similarity
Modified residuei318 – 3181N6-succinyllysine; alternateBy similarity
Modified residuei322 – 3221PhosphothreonineBy similarity

Post-translational modificationi

ISGylated.By similarity

Keywords - PTMi

Acetylation, Phosphoprotein, Ubl conjugation

Proteomic databases

PaxDbiP00339.
PeptideAtlasiP00339.
PRIDEiP00339.

Expressioni

Gene expression databases

BgeeiENSSSCG00000013366.
GenevisibleiP00339. SS.

Interactioni

Subunit structurei

Homotetramer.1 Publication

Protein-protein interaction databases

STRINGi9823.ENSSSCP00000014202.

Structurei

Secondary structure

1
332
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi4 – 85Combined sources
Beta strandi20 – 267Combined sources
Helixi30 – 4112Combined sources
Beta strandi46 – 516Combined sources
Helixi55 – 6713Combined sources
Helixi68 – 714Combined sources
Beta strandi76 – 827Combined sources
Helixi83 – 864Combined sources
Beta strandi90 – 945Combined sources
Helixi106 – 1094Combined sources
Helixi110 – 12718Combined sources
Beta strandi132 – 1354Combined sources
Beta strandi137 – 1393Combined sources
Helixi140 – 15112Combined sources
Beta strandi157 – 1604Combined sources
Helixi164 – 17815Combined sources
Helixi182 – 1843Combined sources
Beta strandi189 – 1913Combined sources
Helixi201 – 2033Combined sources
Helixi211 – 2144Combined sources
Beta strandi219 – 2224Combined sources
Helixi228 – 24518Combined sources
Helixi250 – 26415Combined sources
Beta strandi269 – 2768Combined sources
Turni278 – 2803Combined sources
Beta strandi288 – 2969Combined sources
Beta strandi299 – 3035Combined sources
Helixi310 – 33021Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
9LDBX-ray2.20A/B2-332[»]
9LDTX-ray2.00A/B2-332[»]
ProteinModelPortaliP00339.
SMRiP00339. Positions 2-332.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP00339.

Family & Domainsi

Sequence similaritiesi

Belongs to the LDH/MDH superfamily. LDH family.Curated

Phylogenomic databases

eggNOGiKOG1495. Eukaryota.
COG0039. LUCA.
GeneTreeiENSGT00550000074541.
HOGENOMiHOG000213793.
HOVERGENiHBG000462.
InParanoidiP00339.
OMAiNNEHRVL.
OrthoDBiEOG091G0HME.
TreeFamiTF314963.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
3.90.110.10. 1 hit.
HAMAPiMF_00488. Lactate_dehydrog. 1 hit.
InterProiIPR001557. L-lactate/malate_DH.
IPR011304. L-lactate_DH.
IPR018177. L-lactate_DH_AS.
IPR022383. Lactate/malate_DH_C.
IPR001236. Lactate/malate_DH_N.
IPR015955. Lactate_DH/Glyco_Ohase_4_C.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PANTHERiPTHR11540. PTHR11540. 1 hit.
PfamiPF02866. Ldh_1_C. 1 hit.
PF00056. Ldh_1_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000102. Lac_mal_DH. 1 hit.
PRINTSiPR00086. LLDHDRGNASE.
SUPFAMiSSF51735. SSF51735. 1 hit.
SSF56327. SSF56327. 1 hit.
TIGRFAMsiTIGR01771. L-LDH-NAD. 1 hit.
PROSITEiPS00064. L_LDH. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P00339-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MATLKDQLIH NLLKEEHVPH NKITVVGVGA VGMACAISIL MKELADEIAL
60 70 80 90 100
VDVMEDKLKG EMMDLQHGSL FLRTPKIVSG KDYNVTANSR LVVITAGARQ
110 120 130 140 150
QEGESRLNLV QRNVNIFKFI IPNIVKYSPN CKLLVVSNPV DILTYVAWKI
160 170 180 190 200
SGFPKNRVIG SGCNLDSARF RYLMGERLGV HPLSCHGWIL GEHGDSSVPV
210 220 230 240 250
WSGVNVAGVS LKNLHPELGT DADKEHWKAV HKQVVDSAYE VIKLKGYTSW
260 270 280 290 300
AIGLSVADLA ESIMKNLRRV HPISTMIKGL YGIKEDVFLS VPCILGQNGI
310 320 330
SDVVKVTLTP EEEAHLKKSA DTLWGIQKEL QF
Length:332
Mass (Da):36,619
Last modified:January 23, 2007 - v3
Checksum:iFD3B4673557C9B72
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti233 – 2331Q → E AA sequence (PubMed:838465).Curated
Sequence conflicti286 – 2861D → N AA sequence (PubMed:838465).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U07178 mRNA. Translation: AAA50436.1.
PIRiA00348. DEPGLM.
UniGeneiSsc.50275.

Genome annotation databases

EnsembliENSSSCT00000014597; ENSSSCP00000014202; ENSSSCG00000013366.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U07178 mRNA. Translation: AAA50436.1.
PIRiA00348. DEPGLM.
UniGeneiSsc.50275.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
9LDBX-ray2.20A/B2-332[»]
9LDTX-ray2.00A/B2-332[»]
ProteinModelPortaliP00339.
SMRiP00339. Positions 2-332.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9823.ENSSSCP00000014202.

Proteomic databases

PaxDbiP00339.
PeptideAtlasiP00339.
PRIDEiP00339.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSSSCT00000014597; ENSSSCP00000014202; ENSSSCG00000013366.

Phylogenomic databases

eggNOGiKOG1495. Eukaryota.
COG0039. LUCA.
GeneTreeiENSGT00550000074541.
HOGENOMiHOG000213793.
HOVERGENiHBG000462.
InParanoidiP00339.
OMAiNNEHRVL.
OrthoDBiEOG091G0HME.
TreeFamiTF314963.

Enzyme and pathway databases

UniPathwayiUPA00554; UER00611.
ReactomeiR-SSC-70268. Pyruvate metabolism.

Miscellaneous databases

EvolutionaryTraceiP00339.

Gene expression databases

BgeeiENSSSCG00000013366.
GenevisibleiP00339. SS.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
3.90.110.10. 1 hit.
HAMAPiMF_00488. Lactate_dehydrog. 1 hit.
InterProiIPR001557. L-lactate/malate_DH.
IPR011304. L-lactate_DH.
IPR018177. L-lactate_DH_AS.
IPR022383. Lactate/malate_DH_C.
IPR001236. Lactate/malate_DH_N.
IPR015955. Lactate_DH/Glyco_Ohase_4_C.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PANTHERiPTHR11540. PTHR11540. 1 hit.
PfamiPF02866. Ldh_1_C. 1 hit.
PF00056. Ldh_1_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000102. Lac_mal_DH. 1 hit.
PRINTSiPR00086. LLDHDRGNASE.
SUPFAMiSSF51735. SSF51735. 1 hit.
SSF56327. SSF56327. 1 hit.
TIGRFAMsiTIGR01771. L-LDH-NAD. 1 hit.
PROSITEiPS00064. L_LDH. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiLDHA_PIG
AccessioniPrimary (citable) accession number: P00339
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: January 23, 2007
Last modified: September 7, 2016
This is version 135 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.