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Protein

L-lactate dehydrogenase A chain

Gene

LDHA

Organism
Sus scrofa (Pig)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

(S)-lactate + NAD+ = pyruvate + NADH.

Pathwayi: pyruvate fermentation to lactate

This protein is involved in step 1 of the subpathway that synthesizes (S)-lactate from pyruvate.
Proteins known to be involved in this subpathway in this organism are:
  1. L-lactate dehydrogenase (LDHC), L-lactate dehydrogenase (LDHAL6B), L-lactate dehydrogenase, L-lactate dehydrogenase, L-lactate dehydrogenase (LDHB), L-lactate dehydrogenase A chain (LDHA), L-lactate dehydrogenase C chain (LDHC), L-lactate dehydrogenase B chain (LDHB), L-lactate dehydrogenase (LDHC), L-lactate dehydrogenase, L-lactate dehydrogenase (LDHAL6B), L-lactate dehydrogenase
This subpathway is part of the pathway pyruvate fermentation to lactate, which is itself part of Fermentation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes (S)-lactate from pyruvate, the pathway pyruvate fermentation to lactate and in Fermentation.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei99NAD1 Publication1
Binding sitei106Substrate1
Binding sitei138NAD or substrate1 Publication1
Binding sitei169Substrate1
Active sitei193Proton acceptor1
Binding sitei248Substrate1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi29 – 57NAD1 PublicationAdd BLAST29

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Ligandi

NAD

Enzyme and pathway databases

ReactomeiR-SSC-70268. Pyruvate metabolism.
UniPathwayiUPA00554; UER00611.

Names & Taxonomyi

Protein namesi
Recommended name:
L-lactate dehydrogenase A chain (EC:1.1.1.27)
Short name:
LDH-A
Alternative name(s):
LDH muscle subunit
Short name:
LDH-M
Gene namesi
Name:LDHA
OrganismiSus scrofa (Pig)
Taxonomic identifieri9823 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaSuinaSuidaeSus
Proteomesi
  • UP000008227 Componenti: Chromosome 2

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved1 Publication
ChainiPRO_00001684162 – 332L-lactate dehydrogenase A chainAdd BLAST331

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylalanine1 Publication1
Modified residuei5N6-acetyllysine; alternateBy similarity1
Modified residuei5N6-succinyllysine; alternateBy similarity1
Modified residuei14N6-acetyllysineBy similarity1
Modified residuei57N6-acetyllysineBy similarity1
Modified residuei81N6-acetyllysineBy similarity1
Modified residuei118N6-acetyllysine; alternateBy similarity1
Modified residuei118N6-succinyllysine; alternateBy similarity1
Modified residuei126N6-acetyllysineBy similarity1
Modified residuei224N6-acetyllysineBy similarity1
Modified residuei232N6-acetyllysineBy similarity1
Modified residuei239PhosphotyrosineBy similarity1
Modified residuei243N6-acetyllysineBy similarity1
Modified residuei309PhosphothreonineBy similarity1
Modified residuei318N6-acetyllysine; alternateBy similarity1
Modified residuei318N6-succinyllysine; alternateBy similarity1
Modified residuei322PhosphothreonineBy similarity1

Post-translational modificationi

ISGylated.By similarity

Keywords - PTMi

Acetylation, Phosphoprotein, Ubl conjugation

Proteomic databases

PaxDbiP00339.
PeptideAtlasiP00339.
PRIDEiP00339.

Expressioni

Gene expression databases

BgeeiENSSSCG00000013366.
GenevisibleiP00339. SS.

Interactioni

Subunit structurei

Homotetramer.1 Publication

Protein-protein interaction databases

STRINGi9823.ENSSSCP00000014202.

Structurei

Secondary structure

1332
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi4 – 8Combined sources5
Beta strandi20 – 26Combined sources7
Helixi30 – 41Combined sources12
Beta strandi46 – 51Combined sources6
Helixi55 – 67Combined sources13
Helixi68 – 71Combined sources4
Beta strandi76 – 82Combined sources7
Helixi83 – 86Combined sources4
Beta strandi90 – 94Combined sources5
Helixi106 – 109Combined sources4
Helixi110 – 127Combined sources18
Beta strandi132 – 135Combined sources4
Beta strandi137 – 139Combined sources3
Helixi140 – 151Combined sources12
Beta strandi157 – 160Combined sources4
Helixi164 – 178Combined sources15
Helixi182 – 184Combined sources3
Beta strandi189 – 191Combined sources3
Helixi201 – 203Combined sources3
Helixi211 – 214Combined sources4
Beta strandi219 – 222Combined sources4
Helixi228 – 245Combined sources18
Helixi250 – 264Combined sources15
Beta strandi269 – 276Combined sources8
Turni278 – 280Combined sources3
Beta strandi288 – 296Combined sources9
Beta strandi299 – 303Combined sources5
Helixi310 – 330Combined sources21

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
9LDBX-ray2.20A/B2-332[»]
9LDTX-ray2.00A/B2-332[»]
ProteinModelPortaliP00339.
SMRiP00339.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP00339.

Family & Domainsi

Sequence similaritiesi

Belongs to the LDH/MDH superfamily. LDH family.Curated

Phylogenomic databases

eggNOGiKOG1495. Eukaryota.
COG0039. LUCA.
GeneTreeiENSGT00550000074541.
HOGENOMiHOG000213793.
HOVERGENiHBG000462.
InParanoidiP00339.
OMAiNNEHRVL.
OrthoDBiEOG091G0HME.
TreeFamiTF314963.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
3.90.110.10. 1 hit.
HAMAPiMF_00488. Lactate_dehydrog. 1 hit.
InterProiIPR001557. L-lactate/malate_DH.
IPR011304. L-lactate_DH.
IPR018177. L-lactate_DH_AS.
IPR022383. Lactate/malate_DH_C.
IPR001236. Lactate/malate_DH_N.
IPR015955. Lactate_DH/Glyco_Ohase_4_C.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PANTHERiPTHR11540. PTHR11540. 1 hit.
PfamiPF02866. Ldh_1_C. 1 hit.
PF00056. Ldh_1_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000102. Lac_mal_DH. 1 hit.
PRINTSiPR00086. LLDHDRGNASE.
SUPFAMiSSF51735. SSF51735. 1 hit.
SSF56327. SSF56327. 1 hit.
TIGRFAMsiTIGR01771. L-LDH-NAD. 1 hit.
PROSITEiPS00064. L_LDH. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P00339-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MATLKDQLIH NLLKEEHVPH NKITVVGVGA VGMACAISIL MKELADEIAL
60 70 80 90 100
VDVMEDKLKG EMMDLQHGSL FLRTPKIVSG KDYNVTANSR LVVITAGARQ
110 120 130 140 150
QEGESRLNLV QRNVNIFKFI IPNIVKYSPN CKLLVVSNPV DILTYVAWKI
160 170 180 190 200
SGFPKNRVIG SGCNLDSARF RYLMGERLGV HPLSCHGWIL GEHGDSSVPV
210 220 230 240 250
WSGVNVAGVS LKNLHPELGT DADKEHWKAV HKQVVDSAYE VIKLKGYTSW
260 270 280 290 300
AIGLSVADLA ESIMKNLRRV HPISTMIKGL YGIKEDVFLS VPCILGQNGI
310 320 330
SDVVKVTLTP EEEAHLKKSA DTLWGIQKEL QF
Length:332
Mass (Da):36,619
Last modified:January 23, 2007 - v3
Checksum:iFD3B4673557C9B72
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti233Q → E AA sequence (PubMed:838465).Curated1
Sequence conflicti286D → N AA sequence (PubMed:838465).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U07178 mRNA. Translation: AAA50436.1.
PIRiA00348. DEPGLM.
UniGeneiSsc.50275.

Genome annotation databases

EnsembliENSSSCT00000014597; ENSSSCP00000014202; ENSSSCG00000013366.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U07178 mRNA. Translation: AAA50436.1.
PIRiA00348. DEPGLM.
UniGeneiSsc.50275.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
9LDBX-ray2.20A/B2-332[»]
9LDTX-ray2.00A/B2-332[»]
ProteinModelPortaliP00339.
SMRiP00339.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9823.ENSSSCP00000014202.

Proteomic databases

PaxDbiP00339.
PeptideAtlasiP00339.
PRIDEiP00339.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSSSCT00000014597; ENSSSCP00000014202; ENSSSCG00000013366.

Phylogenomic databases

eggNOGiKOG1495. Eukaryota.
COG0039. LUCA.
GeneTreeiENSGT00550000074541.
HOGENOMiHOG000213793.
HOVERGENiHBG000462.
InParanoidiP00339.
OMAiNNEHRVL.
OrthoDBiEOG091G0HME.
TreeFamiTF314963.

Enzyme and pathway databases

UniPathwayiUPA00554; UER00611.
ReactomeiR-SSC-70268. Pyruvate metabolism.

Miscellaneous databases

EvolutionaryTraceiP00339.

Gene expression databases

BgeeiENSSSCG00000013366.
GenevisibleiP00339. SS.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
3.90.110.10. 1 hit.
HAMAPiMF_00488. Lactate_dehydrog. 1 hit.
InterProiIPR001557. L-lactate/malate_DH.
IPR011304. L-lactate_DH.
IPR018177. L-lactate_DH_AS.
IPR022383. Lactate/malate_DH_C.
IPR001236. Lactate/malate_DH_N.
IPR015955. Lactate_DH/Glyco_Ohase_4_C.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PANTHERiPTHR11540. PTHR11540. 1 hit.
PfamiPF02866. Ldh_1_C. 1 hit.
PF00056. Ldh_1_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000102. Lac_mal_DH. 1 hit.
PRINTSiPR00086. LLDHDRGNASE.
SUPFAMiSSF51735. SSF51735. 1 hit.
SSF56327. SSF56327. 1 hit.
TIGRFAMsiTIGR01771. L-LDH-NAD. 1 hit.
PROSITEiPS00064. L_LDH. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiLDHA_PIG
AccessioniPrimary (citable) accession number: P00339
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: January 23, 2007
Last modified: November 30, 2016
This is version 138 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.