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Protein

L-lactate dehydrogenase B chain

Gene

LDHB

Organism
Sus scrofa (Pig)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

(S)-lactate + NAD+ = pyruvate + NADH.

Pathwayi: pyruvate fermentation to lactate

This protein is involved in step 1 of the subpathway that synthesizes (S)-lactate from pyruvate.
Proteins known to be involved in this subpathway in this organism are:
  1. L-lactate dehydrogenase, L-lactate dehydrogenase (LDHAL6B), L-lactate dehydrogenase, L-lactate dehydrogenase (LDHB), L-lactate dehydrogenase A chain (LDHA), L-lactate dehydrogenase C chain (LDHC), L-lactate dehydrogenase B chain (LDHB), L-lactate dehydrogenase (LDHC), L-lactate dehydrogenase (LDHAL6B), L-lactate dehydrogenase, L-lactate dehydrogenase
This subpathway is part of the pathway pyruvate fermentation to lactate, which is itself part of Fermentation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes (S)-lactate from pyruvate, the pathway pyruvate fermentation to lactate and in Fermentation.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei100 – 1001NADBy similarity
Binding sitei107 – 1071SubstrateBy similarity
Binding sitei139 – 1391NAD or substrateBy similarity
Binding sitei170 – 1701SubstrateBy similarity
Active sitei194 – 1941Proton acceptorBy similarity
Binding sitei249 – 2491SubstrateBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi30 – 5829NADBy similarityAdd
BLAST

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Ligandi

NAD

Enzyme and pathway databases

SABIO-RKP00336.
UniPathwayiUPA00554; UER00611.

Names & Taxonomyi

Protein namesi
Recommended name:
L-lactate dehydrogenase B chain (EC:1.1.1.27)
Short name:
LDH-B
Alternative name(s):
LDH heart subunit
Short name:
LDH-H
Gene namesi
Name:LDHB
OrganismiSus scrofa (Pig)
Taxonomic identifieri9823 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaSuinaSuidaeSus
Proteomesi
  • UP000008227 Componenti: Unplaced

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Chemistry

ChEMBLiCHEMBL3823.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemoved1 Publication
Chaini2 – 334333L-lactate dehydrogenase B chainPRO_0000168462Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylalanine1 Publication
Modified residuei5 – 51N6-acetyllysineBy similarity
Modified residuei7 – 71N6-acetyllysineBy similarity
Modified residuei18 – 181PhosphothreonineBy similarity
Modified residuei44 – 441PhosphoserineBy similarity
Modified residuei58 – 581N6-acetyllysineBy similarity
Modified residuei82 – 821N6-acetyllysineBy similarity
Modified residuei119 – 1191N6-acetyllysineBy similarity
Modified residuei127 – 1271N6-acetyllysineBy similarity
Modified residuei240 – 2401PhosphotyrosineBy similarity
Modified residuei329 – 3291N6-acetyllysineBy similarity

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

PaxDbiP00336.
PeptideAtlasiP00336.

Interactioni

Subunit structurei

Homotetramer.

Protein-protein interaction databases

STRINGi9823.ENSSSCP00000024847.

Chemistry

BindingDBiP00336.

Structurei

Secondary structure

1
334
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi4 – 85Combined sources
Beta strandi12 – 143Combined sources
Beta strandi24 – 274Combined sources
Helixi31 – 4313Combined sources
Beta strandi47 – 515Combined sources
Helixi57 – 6913Combined sources
Helixi70 – 723Combined sources
Beta strandi82 – 854Combined sources
Helixi86 – 883Combined sources
Beta strandi91 – 955Combined sources
Beta strandi103 – 1064Combined sources
Helixi112 – 1198Combined sources
Helixi122 – 1287Combined sources
Beta strandi131 – 1366Combined sources
Beta strandi138 – 1403Combined sources
Helixi141 – 15212Combined sources
Turni155 – 1584Combined sources
Helixi165 – 17915Combined sources
Beta strandi183 – 1853Combined sources
Beta strandi190 – 1978Combined sources
Beta strandi208 – 2114Combined sources
Turni215 – 2184Combined sources
Beta strandi221 – 2266Combined sources
Helixi228 – 24013Combined sources
Helixi247 – 26620Combined sources
Beta strandi280 – 2823Combined sources
Beta strandi285 – 2873Combined sources
Beta strandi294 – 2974Combined sources
Beta strandi300 – 3045Combined sources
Helixi313 – 33119Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
5LDHX-ray2.70A/B2-334[»]
ProteinModelPortaliP00336.
SMRiP00336. Positions 2-333.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP00336.

Family & Domainsi

Sequence similaritiesi

Belongs to the LDH/MDH superfamily. LDH family.Curated

Phylogenomic databases

eggNOGiKOG1495. Eukaryota.
COG0039. LUCA.
HOGENOMiHOG000213793.
HOVERGENiHBG000462.
InParanoidiP00336.
KOiK00016.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
3.90.110.10. 1 hit.
HAMAPiMF_00488. Lactate_dehydrog. 1 hit.
InterProiIPR001557. L-lactate/malate_DH.
IPR011304. L-lactate_DH.
IPR018177. L-lactate_DH_AS.
IPR022383. Lactate/malate_DH_C.
IPR001236. Lactate/malate_DH_N.
IPR015955. Lactate_DH/Glyco_Ohase_4_C.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PANTHERiPTHR11540. PTHR11540. 1 hit.
PfamiPF02866. Ldh_1_C. 1 hit.
PF00056. Ldh_1_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000102. Lac_mal_DH. 1 hit.
PRINTSiPR00086. LLDHDRGNASE.
SUPFAMiSSF51735. SSF51735. 1 hit.
SSF56327. SSF56327. 1 hit.
TIGRFAMsiTIGR01771. L-LDH-NAD. 1 hit.
PROSITEiPS00064. L_LDH. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P00336-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MATLKEKLIA PVAEEETTIP NNKITVVGVG QVGMACAISI LGKSLTDELA
60 70 80 90 100
LVDVLEDKLK GEMMDLQHGS LFLQTPKIVA DKDYSVTANS KIVVVTAGVR
110 120 130 140 150
QQEGESRLNL VQRNVNVFKF IIPQIVKYSP DCIIIVVSNP VDILTYVTWK
160 170 180 190 200
LSGLPKHRVI GSGCNLDSAR FRYLMAEKLG VHPSSCHGWI LGEHGDSSVA
210 220 230 240 250
VWSGVNVAGV SLQELNPEMG TDNDSENWKE VHKMVVESAY EVIKLKGYTN
260 270 280 290 300
WAIGLSVADL IESMLKNLSR IHPVSTMVQG MYGIENEVFL SLPCVLNARG
310 320 330
LTSVINQKLK DDEVAQLKNS ADTLWGIQKD LKDL
Length:334
Mass (Da):36,612
Last modified:January 23, 2007 - v3
Checksum:iA0A2389BAB8FFD73
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti14 – 152EE → QQ AA sequence (PubMed:838465).Curated
Sequence conflicti81 – 811D → N AA sequence (PubMed:838465).Curated
Sequence conflicti131 – 1311D → N AA sequence (PubMed:838465).Curated
Sequence conflicti214 – 2141E → Q AA sequence (PubMed:838465).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U07180 mRNA. Translation: AAA50438.1.
PIRiA91671. DEPGLH.
RefSeqiNP_001106758.1. NM_001113287.1.
UniGeneiSsc.48909.

Genome annotation databases

GeneIDi100621540.
KEGGissc:100621540.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U07180 mRNA. Translation: AAA50438.1.
PIRiA91671. DEPGLH.
RefSeqiNP_001106758.1. NM_001113287.1.
UniGeneiSsc.48909.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
5LDHX-ray2.70A/B2-334[»]
ProteinModelPortaliP00336.
SMRiP00336. Positions 2-333.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9823.ENSSSCP00000024847.

Chemistry

BindingDBiP00336.
ChEMBLiCHEMBL3823.

Proteomic databases

PaxDbiP00336.
PeptideAtlasiP00336.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi100621540.
KEGGissc:100621540.

Organism-specific databases

CTDi3945.

Phylogenomic databases

eggNOGiKOG1495. Eukaryota.
COG0039. LUCA.
HOGENOMiHOG000213793.
HOVERGENiHBG000462.
InParanoidiP00336.
KOiK00016.

Enzyme and pathway databases

UniPathwayiUPA00554; UER00611.
SABIO-RKP00336.

Miscellaneous databases

EvolutionaryTraceiP00336.
PROiP00336.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
3.90.110.10. 1 hit.
HAMAPiMF_00488. Lactate_dehydrog. 1 hit.
InterProiIPR001557. L-lactate/malate_DH.
IPR011304. L-lactate_DH.
IPR018177. L-lactate_DH_AS.
IPR022383. Lactate/malate_DH_C.
IPR001236. Lactate/malate_DH_N.
IPR015955. Lactate_DH/Glyco_Ohase_4_C.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PANTHERiPTHR11540. PTHR11540. 1 hit.
PfamiPF02866. Ldh_1_C. 1 hit.
PF00056. Ldh_1_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000102. Lac_mal_DH. 1 hit.
PRINTSiPR00086. LLDHDRGNASE.
SUPFAMiSSF51735. SSF51735. 1 hit.
SSF56327. SSF56327. 1 hit.
TIGRFAMsiTIGR01771. L-LDH-NAD. 1 hit.
PROSITEiPS00064. L_LDH. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiLDHB_PIG
AccessioniPrimary (citable) accession number: P00336
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: January 23, 2007
Last modified: July 6, 2016
This is version 133 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.