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Protein

L-lactate dehydrogenase B chain

Gene

LDHB

Organism
Sus scrofa (Pig)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

(S)-lactate + NAD+ = pyruvate + NADH.

Pathwayi: pyruvate fermentation to lactate

This protein is involved in step 1 of the subpathway that synthesizes (S)-lactate from pyruvate.
Proteins known to be involved in this subpathway in this organism are:
  1. L-lactate dehydrogenase (LDHC), L-lactate dehydrogenase (LDHAL6B), L-lactate dehydrogenase, L-lactate dehydrogenase, L-lactate dehydrogenase (LDHB), L-lactate dehydrogenase A chain (LDHA), L-lactate dehydrogenase C chain (LDHC), L-lactate dehydrogenase B chain (LDHB), L-lactate dehydrogenase (LDHC), L-lactate dehydrogenase (LDHAL6B), L-lactate dehydrogenase, L-lactate dehydrogenase
This subpathway is part of the pathway pyruvate fermentation to lactate, which is itself part of Fermentation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes (S)-lactate from pyruvate, the pathway pyruvate fermentation to lactate and in Fermentation.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei100NADBy similarity1
Binding sitei107SubstrateBy similarity1
Binding sitei139NAD or substrateBy similarity1
Binding sitei170SubstrateBy similarity1
Active sitei194Proton acceptorBy similarity1
Binding sitei249SubstrateBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi30 – 58NADBy similarityAdd BLAST29

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Ligandi

NAD

Enzyme and pathway databases

SABIO-RKP00336.
UniPathwayiUPA00554; UER00611.

Names & Taxonomyi

Protein namesi
Recommended name:
L-lactate dehydrogenase B chain (EC:1.1.1.27)
Short name:
LDH-B
Alternative name(s):
LDH heart subunit
Short name:
LDH-H
Gene namesi
Name:LDHB
OrganismiSus scrofa (Pig)
Taxonomic identifieri9823 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaSuinaSuidaeSus
Proteomesi
  • UP000008227 Componenti: Unplaced

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Chemistry databases

ChEMBLiCHEMBL3823.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved1 Publication
ChainiPRO_00001684622 – 334L-lactate dehydrogenase B chainAdd BLAST333

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylalanine1 Publication1
Modified residuei5N6-acetyllysineBy similarity1
Modified residuei7N6-acetyllysineBy similarity1
Modified residuei18PhosphothreonineBy similarity1
Modified residuei44PhosphoserineBy similarity1
Modified residuei58N6-acetyllysineBy similarity1
Modified residuei82N6-acetyllysineBy similarity1
Modified residuei119N6-acetyllysineBy similarity1
Modified residuei127N6-acetyllysineBy similarity1
Modified residuei240PhosphotyrosineBy similarity1
Modified residuei329N6-acetyllysineBy similarity1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

PaxDbiP00336.
PeptideAtlasiP00336.
PRIDEiP00336.

Interactioni

Subunit structurei

Homotetramer.

Protein-protein interaction databases

STRINGi9823.ENSSSCP00000024847.

Chemistry databases

BindingDBiP00336.

Structurei

Secondary structure

1334
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi4 – 8Combined sources5
Beta strandi12 – 14Combined sources3
Beta strandi24 – 27Combined sources4
Helixi31 – 43Combined sources13
Beta strandi47 – 51Combined sources5
Helixi57 – 69Combined sources13
Helixi70 – 72Combined sources3
Beta strandi82 – 85Combined sources4
Helixi86 – 88Combined sources3
Beta strandi91 – 95Combined sources5
Beta strandi103 – 106Combined sources4
Helixi112 – 119Combined sources8
Helixi122 – 128Combined sources7
Beta strandi131 – 136Combined sources6
Beta strandi138 – 140Combined sources3
Helixi141 – 152Combined sources12
Turni155 – 158Combined sources4
Helixi165 – 179Combined sources15
Beta strandi183 – 185Combined sources3
Beta strandi190 – 197Combined sources8
Beta strandi208 – 211Combined sources4
Turni215 – 218Combined sources4
Beta strandi221 – 226Combined sources6
Helixi228 – 240Combined sources13
Helixi247 – 266Combined sources20
Beta strandi280 – 282Combined sources3
Beta strandi285 – 287Combined sources3
Beta strandi294 – 297Combined sources4
Beta strandi300 – 304Combined sources5
Helixi313 – 331Combined sources19

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
5LDHX-ray2.70A/B2-334[»]
ProteinModelPortaliP00336.
SMRiP00336.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP00336.

Family & Domainsi

Sequence similaritiesi

Belongs to the LDH/MDH superfamily. LDH family.Curated

Phylogenomic databases

eggNOGiKOG1495. Eukaryota.
COG0039. LUCA.
HOGENOMiHOG000213793.
HOVERGENiHBG000462.
InParanoidiP00336.
KOiK00016.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
3.90.110.10. 1 hit.
HAMAPiMF_00488. Lactate_dehydrog. 1 hit.
InterProiIPR001557. L-lactate/malate_DH.
IPR011304. L-lactate_DH.
IPR018177. L-lactate_DH_AS.
IPR022383. Lactate/malate_DH_C.
IPR001236. Lactate/malate_DH_N.
IPR015955. Lactate_DH/Glyco_Ohase_4_C.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PANTHERiPTHR11540. PTHR11540. 1 hit.
PfamiPF02866. Ldh_1_C. 1 hit.
PF00056. Ldh_1_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000102. Lac_mal_DH. 1 hit.
PRINTSiPR00086. LLDHDRGNASE.
SUPFAMiSSF51735. SSF51735. 1 hit.
SSF56327. SSF56327. 1 hit.
TIGRFAMsiTIGR01771. L-LDH-NAD. 1 hit.
PROSITEiPS00064. L_LDH. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P00336-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MATLKEKLIA PVAEEETTIP NNKITVVGVG QVGMACAISI LGKSLTDELA
60 70 80 90 100
LVDVLEDKLK GEMMDLQHGS LFLQTPKIVA DKDYSVTANS KIVVVTAGVR
110 120 130 140 150
QQEGESRLNL VQRNVNVFKF IIPQIVKYSP DCIIIVVSNP VDILTYVTWK
160 170 180 190 200
LSGLPKHRVI GSGCNLDSAR FRYLMAEKLG VHPSSCHGWI LGEHGDSSVA
210 220 230 240 250
VWSGVNVAGV SLQELNPEMG TDNDSENWKE VHKMVVESAY EVIKLKGYTN
260 270 280 290 300
WAIGLSVADL IESMLKNLSR IHPVSTMVQG MYGIENEVFL SLPCVLNARG
310 320 330
LTSVINQKLK DDEVAQLKNS ADTLWGIQKD LKDL
Length:334
Mass (Da):36,612
Last modified:January 23, 2007 - v3
Checksum:iA0A2389BAB8FFD73
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti14 – 15EE → QQ AA sequence (PubMed:838465).Curated2
Sequence conflicti81D → N AA sequence (PubMed:838465).Curated1
Sequence conflicti131D → N AA sequence (PubMed:838465).Curated1
Sequence conflicti214E → Q AA sequence (PubMed:838465).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U07180 mRNA. Translation: AAA50438.1.
PIRiA91671. DEPGLH.
RefSeqiNP_001106758.1. NM_001113287.1.
UniGeneiSsc.48909.

Genome annotation databases

GeneIDi100621540.
KEGGissc:100621540.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U07180 mRNA. Translation: AAA50438.1.
PIRiA91671. DEPGLH.
RefSeqiNP_001106758.1. NM_001113287.1.
UniGeneiSsc.48909.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
5LDHX-ray2.70A/B2-334[»]
ProteinModelPortaliP00336.
SMRiP00336.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9823.ENSSSCP00000024847.

Chemistry databases

BindingDBiP00336.
ChEMBLiCHEMBL3823.

Proteomic databases

PaxDbiP00336.
PeptideAtlasiP00336.
PRIDEiP00336.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi100621540.
KEGGissc:100621540.

Organism-specific databases

CTDi3945.

Phylogenomic databases

eggNOGiKOG1495. Eukaryota.
COG0039. LUCA.
HOGENOMiHOG000213793.
HOVERGENiHBG000462.
InParanoidiP00336.
KOiK00016.

Enzyme and pathway databases

UniPathwayiUPA00554; UER00611.
SABIO-RKP00336.

Miscellaneous databases

EvolutionaryTraceiP00336.
PROiP00336.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
3.90.110.10. 1 hit.
HAMAPiMF_00488. Lactate_dehydrog. 1 hit.
InterProiIPR001557. L-lactate/malate_DH.
IPR011304. L-lactate_DH.
IPR018177. L-lactate_DH_AS.
IPR022383. Lactate/malate_DH_C.
IPR001236. Lactate/malate_DH_N.
IPR015955. Lactate_DH/Glyco_Ohase_4_C.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PANTHERiPTHR11540. PTHR11540. 1 hit.
PfamiPF02866. Ldh_1_C. 1 hit.
PF00056. Ldh_1_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000102. Lac_mal_DH. 1 hit.
PRINTSiPR00086. LLDHDRGNASE.
SUPFAMiSSF51735. SSF51735. 1 hit.
SSF56327. SSF56327. 1 hit.
TIGRFAMsiTIGR01771. L-LDH-NAD. 1 hit.
PROSITEiPS00064. L_LDH. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiLDHB_PIG
AccessioniPrimary (citable) accession number: P00336
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: January 23, 2007
Last modified: November 2, 2016
This is version 135 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.