Reviewed, UniProtKB/Swiss-Prot P00335 (RIDH_KLEAE)

Names and origin

Protein names

Recommended name:
    Ribitol 2-dehydrogenase
      Short name=RDH
    EC=1.1.1.56

Gene names

Name:

rbtD

Organism

Klebsiella aerogenes

Taxonomic identifier

28451 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Klebsiella

Protein attributes

Sequence length	249 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Catalytic activity	Ribitol + NAD+ = D-ribulose + NADH.
Subunit structure	Homotetramer.
Miscellaneous	The sequence shown is that of strain A.
Sequence similarities	Belongs to the short-chain dehydrogenases/reductases (SDR) family.

Ontologies

Keywords
Ligand	NAD
Molecular function	Oxidoreductase
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	binding Inferred from electronic annotation. Source: InterPro ribitol 2-dehydrogenase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 249	249	Ribitol 2-dehydrogenase		PRO_0000054756
Regions
	Nucleotide binding	20 – 43	24	NAD By similarity
Sites
	Active site	160	1	Proton acceptor By similarity
Natural variations
	Natural variant	196	1	A → P in strain: D.
Experimental info
	Sequence conflict	146 – 147	2	AV → SS in AAA25140. Ref.2
	Sequence conflict	212	1	N → D AA sequence Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P00335-1 [UniParc]. Last modified August 1, 1988. Version 1. Checksum: C34896C276CC8FBC	FASTA	249	26,514
	10 20 30 40 50 60 MKHSVSSMNT SLSGKVAAIT GAASGIGLEC ARTLLGAGAK VVLIDREGEK LNKLVAELGE 70 80 90 100 110 120 NAFALQVDLM QADQVDNLLQ GILQLTGRLD IFHANAGAYI GGPVAEGDPD VWDRVLHLNI 130 140 150 160 170 180 NAAFRCVRSV LPHLIAQKSG DIIFTAVIAG VVPVIWEPVY TASKFAVQAF VHTTRRQVAQ 190 200 210 220 230 240 YGVRVGAVLP GPVVTALLDD WPKAKMDEAL ANGSLMQPIE VAESVLFMVT RSKNVTVRDI VILPNSVDL

References

[1]	"Ribitol dehydrogenase of Klebsiella aerogenes. Sequence and properties of wild-type and mutant strains." Dothie J.M., Giglio J.R., Moore C.H., Taylor S.S., Hartley B.S. Biochem. J. 230:569-578(1985) [PubMed: 3904726] [Abstract] Cited for: PROTEIN SEQUENCE. Strain: A and D.
[2]	"Ribitol dehydrogenase of Klebsiella aerogenes. Sequence of the structural gene." Loviny T., Norton P.M., Hartley B.S. Biochem. J. 230:579-585(1985) [PubMed: 2933028] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"Structure of wild-type and mutant repressors and of the control region of the rbt operon of Klebsiella aerogenes." Wu J.C., Anderton-Loviny T., Smith C.A., Hartley B.S. EMBO J. 4:1339-1344(1985) [PubMed: 3891331] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-68.

Cross-references

Sequence databases
EMBLGenBankDDBJ	M25606 Genomic DNA. Translation: AAA25140.1. X02448 Genomic DNA. Translation: CAA26292.1.
PIR	DEKBR. A94585. S07134.
3D structure databases
HSSP	HSSP built from PDB template 1JA9 based on UniProtKB Q9HFV6.
ModBase	Search...
Enzyme and pathway databases
BioCyc	MetaCyc:MON-12237.
BRENDA	1.1.1.56. 366.
Family and domain databases
InterPro	IPR002198. DH_sc/Rdtase_SDR. IPR002347. Glc/ribitol_DH. IPR016040. NAD(P)-bd_dom. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
PANTHER	PTHR19410. ADH_short_C2. 1 hit.
Pfam	PF00106. adh_short. 1 hit. [Graphical view]
PRINTS	PR00081. GDHRDH.
PROSITE	PS00061. ADH_SHORT. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

RIDH_KLEAE

Accession

Primary (citable) accession number: P00335

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 21, 1986
Last sequence update:	August 1, 1988
Last modified:	June 16, 2009
This is version 67 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)