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Protein

Alcohol dehydrogenase

Gene

Adh

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

An alcohol + NAD+ = an aldehyde or ketone + NADH.PROSITE-ProRule annotation

Enzyme regulationi

Inhibited by 2,2,2-trifluoroethanol and pyrazole.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei65NAD1 Publication1
Binding sitei140Substrate1 Publication1
Active sitei153Proton acceptor1 Publication1
Binding sitei157NAD1 Publication1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi12 – 41NAD1 PublicationAdd BLAST30

GO - Molecular functioni

  • acetaldehyde dehydrogenase (acetylating) activity Source: FlyBase
  • alcohol dehydrogenase (NAD) activity Source: UniProtKB
  • protein homodimerization activity Source: FlyBase

GO - Biological processi

  • acetaldehyde metabolic process Source: FlyBase
  • alcohol catabolic process Source: UniProtKB
  • alcohol metabolic process Source: FlyBase
  • behavioral response to ethanol Source: FlyBase
  • ethanol metabolic process Source: FlyBase
  • ethanol oxidation Source: FlyBase
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Ligandi

NAD

Enzyme and pathway databases

ReactomeiR-DME-2142700. Synthesis of Lipoxins (LX).
R-DME-77111. Synthesis of Ketone Bodies.
SABIO-RKP00334.

Names & Taxonomyi

Protein namesi
Recommended name:
Alcohol dehydrogenase (EC:1.1.1.1)
Gene namesi
Name:Adh
ORF Names:CG3481
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
Proteomesi
  • UP000000803 Componenti: Chromosome 2L

Organism-specific databases

FlyBaseiFBgn0000055. Adh.

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: FlyBase
  • lipid particle Source: FlyBase
  • microtubule associated complex Source: FlyBase
  • protein complex Source: FlyBase
Complete GO annotation...

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi15G → A: 31% decrease in activity. 1 Publication1
Mutagenesisi15G → D in Adh-N11; loss of activity. 1 Publication1
Mutagenesisi15G → V: Complete loss of activity. 1 Publication1
Mutagenesisi130G → C: Complete loss of activity. 1 Publication1
Mutagenesisi133G → I: Complete loss of activity. 1 Publication1
Mutagenesisi136C → A: No decrease in activity. 1 Publication1
Mutagenesisi153Y → C: Retains only 0.25% activity. 3 Publications1
Mutagenesisi153Y → F, H, E or Q: Complete loss of activity. 3 Publications1
Mutagenesisi157K → I: Complete loss of activity. 2 Publications1
Mutagenesisi157K → R: Retains only 2.2% activity. 2 Publications1
Mutagenesisi184G → L: Complete loss of activity. 1 Publication1
Mutagenesisi219C → A: No decrease in activity. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved2 Publications
ChainiPRO_00000544742 – 256Alcohol dehydrogenaseAdd BLAST255

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylserine1 Publication1

Keywords - PTMi

Acetylation

Proteomic databases

PaxDbiP00334.
PeptideAtlasiP00334.
PRIDEiP00334.

Expressioni

Gene expression databases

BgeeiFBgn0000055.
ExpressionAtlasiP00334. baseline.
GenevisibleiP00334. DM.

Interactioni

Subunit structurei

Homodimer.1 Publication

GO - Molecular functioni

  • protein homodimerization activity Source: FlyBase

Protein-protein interaction databases

BioGridi533474. 59 interactors.
IntActiP00334. 19 interactors.
MINTiMINT-877153.
STRINGi7227.FBpp0100048.

Structurei

Secondary structure

1256
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi9 – 13Combined sources5
Turni14 – 16Combined sources3
Helixi18 – 27Combined sources10
Beta strandi33 – 40Combined sources8
Helixi44 – 53Combined sources10
Beta strandi57 – 63Combined sources7
Helixi70 – 84Combined sources15
Beta strandi89 – 92Combined sources4
Helixi102 – 109Combined sources8
Helixi111 – 124Combined sources14
Helixi126 – 128Combined sources3
Beta strandi133 – 138Combined sources6
Helixi141 – 143Combined sources3
Helixi151 – 174Combined sources24
Beta strandi175 – 183Combined sources9
Beta strandi185 – 188Combined sources4
Turni189 – 192Combined sources4
Helixi197 – 199Combined sources3
Helixi204 – 209Combined sources6
Helixi216 – 229Combined sources14
Beta strandi235 – 239Combined sources5
Beta strandi242 – 245Combined sources4

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1MG5X-ray1.63A/B2-256[»]
ProteinModelPortaliP00334.
SMRiP00334.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP00334.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG4169. Eukaryota.
COG1028. LUCA.
InParanoidiP00334.
KOiK00001.
OrthoDBiEOG091G0OC9.
PhylomeDBiP00334.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
InterProiIPR002425. ADH_Drosophila-type.
IPR002424. ADH_insect.
IPR016040. NAD(P)-bd_dom.
IPR020904. Sc_DH/Rdtase_CS.
IPR002347. SDR_fam.
[Graphical view]
PANTHERiPTHR24322. PTHR24322. 2 hits.
PfamiPF00106. adh_short. 1 hit.
[Graphical view]
PRINTSiPR01168. ALCDHDRGNASE.
PR01167. INSADHFAMILY.
PR00080. SDRFAMILY.
SUPFAMiSSF51735. SSF51735. 1 hit.
PROSITEiPS00061. ADH_SHORT. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P00334-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSFTLTNKNV IFVAGLGGIG LDTSKELLKR DLKNLVILDR IENPAAIAEL
60 70 80 90 100
KAINPKVTVT FYPYDVTVPI AETTKLLKTI FAQLKTVDVL INGAGILDDH
110 120 130 140 150
QIERTIAVNY TGLVNTTTAI LDFWDKRKGG PGGIICNIGS VTGFNAIYQV
160 170 180 190 200
PVYSGTKAAV VNFTSSLAKL APITGVTAYT VNPGITRTTL VHKFNSWLDV
210 220 230 240 250
EPQVAEKLLA HPTQPSLACA ENFVKAIELN QNGAIWKLDL GTLEAIQWTK

HWDSGI
Length:256
Mass (Da):27,761
Last modified:January 23, 2007 - v2
Checksum:iEB4DF91029007284
GO

Polymorphismi

Virtually all natural populations of this species are polymorphic for 2 electrophoretically distinguishable alleles, Adh-S and Adh-F (PubMed:6789320, PubMed:6410283, PubMed:1673107, PubMed:1683848, Ref. 12, PubMed:12537569, Ref. 18, PubMed:6821373, PubMed:6780981). The sequence of the Adh-S allele is shown (PubMed:6789320, PubMed:6410283, PubMed:1673107, Ref. 12, PubMed:12537569, Ref. 18, PubMed:6821373). Other naturally occurring alleles include Adh-JA-F, Adh-AF-S, Adh-F-CHD, Adh-71K, Adh-UF and Adh-F' (PubMed:3021568, PubMed:3137352, PubMed:2124644, PubMed:6789328, PubMed:115502, PubMed:6821373). Artificially induced mutations include Adh-NB, Adh-NLA248, Adh-N4 and Adh-N11 (PubMed:6818527, PubMed:3928896, PubMed:3108863, PubMed:6821373, PubMed:2419573).18 Publications

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural varianti9N → V in allele Adh-UF. 1
Natural varianti46A → D in allele Adh-UF. 1
Natural varianti46A → V in strain: NC16. 1
Natural varianti52A → E in allele Adh-F'. 1
Natural varianti71A → S in strain: NC16. 1
Natural varianti193K → T in allele Adh-F, allele Adh-F-CHD, allele Adh-71K, allele Adh-JA-F and in strain Berkeley. 1
Natural varianti215P → S in allele Adh-F-CHD and allele Adh-71K. 1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M17827 Genomic DNA. Translation: AAA28341.1.
M17828 Genomic DNA. Translation: AAA28342.1.
M19547 Genomic DNA. Translation: AAA70210.1.
M17830 Genomic DNA. Translation: AAA28343.1.
M17831 Genomic DNA. Translation: AAA28344.1.
M17832 Genomic DNA. Translation: AAA28345.1.
M17833 Genomic DNA. Translation: AAA28346.1.
M17834 Genomic DNA. Translation: AAA28347.1.
M17835 Genomic DNA. Translation: AAA28348.1.
M17836 Genomic DNA. Translation: AAA28349.1.
M17837 Genomic DNA. Translation: AAA70212.1.
Z00030 Genomic DNA. Translation: CAA77330.1.
M22210 Genomic DNA. Translation: AAA28338.1.
M57239 Genomic DNA. Translation: AAA28330.1.
X78384 Genomic DNA. Translation: CAA55151.1.
M36580 Genomic DNA. Translation: AAA28331.1.
X60791 Genomic DNA. Translation: CAA43204.1.
X60792 Genomic DNA. Translation: CAA43205.1.
X60793 Genomic DNA. Translation: CAA43206.1.
U20765 Genomic DNA. Translation: AAA88817.1.
X98338 mRNA. Translation: CAA66981.1.
AF175211 Genomic DNA. Translation: AAF00229.1.
AF175212 Genomic DNA. Translation: AAF00230.1.
AF175213 Genomic DNA. Translation: AAF00231.1.
AF175214 Genomic DNA. Translation: AAF00232.1.
AF175215 Genomic DNA. Translation: AAF00233.1.
AF175216 Genomic DNA. Translation: AAF00234.1.
AF175217 Genomic DNA. Translation: AAF00235.1.
AF175218 Genomic DNA. Translation: AAF00236.1.
AF175219 Genomic DNA. Translation: AAF00237.1.
AF175220 Genomic DNA. Translation: AAF00238.1.
AE014134 Genomic DNA. Translation: AAO41197.1.
AE014134 Genomic DNA. Translation: AAO41199.1.
AE014134 Genomic DNA. Translation: AAO41200.1.
AE014134 Genomic DNA. Translation: AAO41201.1.
AY060227 mRNA. Translation: AAL25266.1.
BT012435 mRNA. Translation: AAS93706.1.
M17845 Genomic DNA. Translation: AAA28363.1.
PIRiA93309. DEFFA.
RefSeqiNP_001027266.1. NM_001032095.2.
NP_001027267.1. NM_001032096.2.
NP_001027268.1. NM_001032097.2.
NP_001027269.1. NM_001032098.2.
NP_001027270.1. NM_001032099.2.
UniGeneiDm.6818.

Genome annotation databases

GeneIDi3771877.
KEGGidme:Dmel_CG3481.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M17827 Genomic DNA. Translation: AAA28341.1.
M17828 Genomic DNA. Translation: AAA28342.1.
M19547 Genomic DNA. Translation: AAA70210.1.
M17830 Genomic DNA. Translation: AAA28343.1.
M17831 Genomic DNA. Translation: AAA28344.1.
M17832 Genomic DNA. Translation: AAA28345.1.
M17833 Genomic DNA. Translation: AAA28346.1.
M17834 Genomic DNA. Translation: AAA28347.1.
M17835 Genomic DNA. Translation: AAA28348.1.
M17836 Genomic DNA. Translation: AAA28349.1.
M17837 Genomic DNA. Translation: AAA70212.1.
Z00030 Genomic DNA. Translation: CAA77330.1.
M22210 Genomic DNA. Translation: AAA28338.1.
M57239 Genomic DNA. Translation: AAA28330.1.
X78384 Genomic DNA. Translation: CAA55151.1.
M36580 Genomic DNA. Translation: AAA28331.1.
X60791 Genomic DNA. Translation: CAA43204.1.
X60792 Genomic DNA. Translation: CAA43205.1.
X60793 Genomic DNA. Translation: CAA43206.1.
U20765 Genomic DNA. Translation: AAA88817.1.
X98338 mRNA. Translation: CAA66981.1.
AF175211 Genomic DNA. Translation: AAF00229.1.
AF175212 Genomic DNA. Translation: AAF00230.1.
AF175213 Genomic DNA. Translation: AAF00231.1.
AF175214 Genomic DNA. Translation: AAF00232.1.
AF175215 Genomic DNA. Translation: AAF00233.1.
AF175216 Genomic DNA. Translation: AAF00234.1.
AF175217 Genomic DNA. Translation: AAF00235.1.
AF175218 Genomic DNA. Translation: AAF00236.1.
AF175219 Genomic DNA. Translation: AAF00237.1.
AF175220 Genomic DNA. Translation: AAF00238.1.
AE014134 Genomic DNA. Translation: AAO41197.1.
AE014134 Genomic DNA. Translation: AAO41199.1.
AE014134 Genomic DNA. Translation: AAO41200.1.
AE014134 Genomic DNA. Translation: AAO41201.1.
AY060227 mRNA. Translation: AAL25266.1.
BT012435 mRNA. Translation: AAS93706.1.
M17845 Genomic DNA. Translation: AAA28363.1.
PIRiA93309. DEFFA.
RefSeqiNP_001027266.1. NM_001032095.2.
NP_001027267.1. NM_001032096.2.
NP_001027268.1. NM_001032097.2.
NP_001027269.1. NM_001032098.2.
NP_001027270.1. NM_001032099.2.
UniGeneiDm.6818.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1MG5X-ray1.63A/B2-256[»]
ProteinModelPortaliP00334.
SMRiP00334.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi533474. 59 interactors.
IntActiP00334. 19 interactors.
MINTiMINT-877153.
STRINGi7227.FBpp0100048.

Proteomic databases

PaxDbiP00334.
PeptideAtlasiP00334.
PRIDEiP00334.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi3771877.
KEGGidme:Dmel_CG3481.

Organism-specific databases

CTDi3771877.
FlyBaseiFBgn0000055. Adh.

Phylogenomic databases

eggNOGiKOG4169. Eukaryota.
COG1028. LUCA.
InParanoidiP00334.
KOiK00001.
OrthoDBiEOG091G0OC9.
PhylomeDBiP00334.

Enzyme and pathway databases

ReactomeiR-DME-2142700. Synthesis of Lipoxins (LX).
R-DME-77111. Synthesis of Ketone Bodies.
SABIO-RKP00334.

Miscellaneous databases

EvolutionaryTraceiP00334.
GenomeRNAii3771877.
PROiP00334.

Gene expression databases

BgeeiFBgn0000055.
ExpressionAtlasiP00334. baseline.
GenevisibleiP00334. DM.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
InterProiIPR002425. ADH_Drosophila-type.
IPR002424. ADH_insect.
IPR016040. NAD(P)-bd_dom.
IPR020904. Sc_DH/Rdtase_CS.
IPR002347. SDR_fam.
[Graphical view]
PANTHERiPTHR24322. PTHR24322. 2 hits.
PfamiPF00106. adh_short. 1 hit.
[Graphical view]
PRINTSiPR01168. ALCDHDRGNASE.
PR01167. INSADHFAMILY.
PR00080. SDRFAMILY.
SUPFAMiSSF51735. SSF51735. 1 hit.
PROSITEiPS00061. ADH_SHORT. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiADH_DROME
AccessioniPrimary (citable) accession number: P00334
Secondary accession number(s): A4V0Q1
, Q27332, Q27579, Q27596, Q2MGK2, Q53XD5, Q95TC8, Q9NKC0, Q9VJS3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: January 23, 2007
Last modified: November 30, 2016
This is version 179 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.