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Reviewed, UniProtKB/Swiss-Prot P00333 (ADH1_MAIZE)

Last modified June 16, 2009. Version 81. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein namesRecommended name:
    Alcohol dehydrogenase 1
    EC=1.1.1.1
Gene names
Name: ADH1
OrganismZea mays (Maize)
Taxonomic identifier4577 [NCBI]
Taxonomic lineageEukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › Liliopsida › Poales › Poaceae › PACCAD clade › Panicoideae › Andropogoneae › Zea

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Protein attributes

Sequence length379 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is not processed.
Protein existenceEvidence at transcript level.

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General annotation (Comments)

Catalytic activity

An alcohol + NAD+ = an aldehyde or ketone + NADH.

Cofactor

Binds 2 zinc ions per subunit.

Subunit structure

Homodimer.

Subcellular location

Cytoplasm.

Polymorphism

The sequence shown is that of allele ADH1-F.

Miscellaneous

In maize there are two isozymes.

Sequence similarities

Belongs to the zinc-containing alcohol dehydrogenase family.

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Ontologies

Keywords
   Cellular componentCytoplasm
   Coding sequence diversityPolymorphism
   LigandMetal-binding
NAD
Zinc
   Molecular functionOxidoreductase
Gene Ontology (GO)
   Biological processoxidation reduction

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular functionalcohol dehydrogenase activity

Inferred from electronic annotation. Source: EC

zinc ion binding

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

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Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 379379Alcohol dehydrogenase 1
PRO_0000160703

Regions

Nucleotide binding202 – 2076NAD By similarity
Nucleotide binding295 – 2973NAD By similarity

Sites

Metal binding471Zinc 1; catalytic By similarity
Metal binding691Zinc 1; catalytic By similarity
Metal binding991Zinc 2 By similarity
Metal binding1021Zinc 2 By similarity
Metal binding1051Zinc 2 By similarity
Metal binding1131Zinc 2 By similarity
Metal binding1771Zinc 1; catalytic By similarity
Binding site2261NAD By similarity
Binding site2311NAD By similarity
Binding site3721NAD By similarity

Natural variations

Natural variant521Y → D in allele ADH1-Cm.
Natural variant1271A → G in allele ADH1-S.
Natural variant1791Y → I in allele ADH1-Cm and allele ADH1-S.
Natural variant3631D → N in allele ADH1-S.

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Sequences

Sequence LengthMass (Da)Tools
P00333-1 [UniParc].

Last modified August 13, 1987. Version 1.
Checksum: 5E0618F7E1A4B973

FASTA37940,981
        10         20         30         40         50         60 
MATAGKVIKC KAAVAWEAGK PLSIEEVEVA PPQAMEVRVK ILFTSLCHTD VYFWEAKGQT 

        70         80         90        100        110        120 
PVFPRIFGHE AGGIIESVGE GVTDVAPGDH VLPVFTGECK ECAHCKSAES NMCDLLRINT 

       130        140        150        160        170        180 
DRGVMIADGK SRFSINGKPI YHFVGTSTFS EYTVMHVGCV AKINPQAPLD KVCVLSCGYS 

       190        200        210        220        230        240 
TGLGASINVA KPPKGSTVAV FGLGAVGLAA AEGARIAGAS RIIGVDLNPS RFEEARKFGC 

       250        260        270        280        290        300 
TEFVNPKDHN KPVQEVLAEM TNGGVDRSVE CTGNINAMIQ AFECVHDGWG VAVLVGVPHK 

       310        320        330        340        350        360 
DAEFKTHPMN FLNERTLKGT FFGNYKPRTD LPNVVELYMK KELEVEKFIT HSVPFAEINK 

       370 
AFDLMAKGEG IRCIIRMEN

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References

[1]"Molecular analysis of the alcohol dehydrogenase (Adh1) gene of maize."
Dennis E.S., Gerlach W.L., Pryor A.J., Bennetzen J.L., Inglis A., Llewellyn D.J., Sachs M.M., Ferl R.J., Peacock W.J.
Nucleic Acids Res. 12:3983-4000(1984) [PubMed: 6328449] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]"Molecular analysis of the alcohol dehydrogenase 2 (Adh2) gene of maize."
Dennis E.S., Sachs M.M., Gerlach W.L., Finnegan E.J., Peacock W.J.
Nucleic Acids Res. 13:727-743(1985) [PubMed: 2987807] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE.
[3]"Correlation of exons with structural domains in alcohol dehydrogenase."
Braenden C.-I., Eklund H., Cambillau C., Pryor A.J.
EMBO J. 3:1307-1310(1984) [PubMed: 6378620] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE.
[4]"Two alleles of maize alcohol dehydrogenase 1 have 3'-structural and poly(A) addition polymorphisms."
Sachs M.M., Dennis E.S., Gerlach W.L., Peacock W.J.
Genetics 113:449-467(1986) [PubMed: 17246333] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE (ALLELE ADH1-F).
Strain: cv. Berkeley Fast.
[5]"Molecular analysis of the ADH1-Cm allele of maize."
Osterman J.C., Dennis E.S.
Plant Mol. Biol. 13:203-212(1989) [PubMed: 2577507] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE (ALLELE ADH1-CM).
[6]"cDNA cloning and induction of the alcohol dehydrogenase gene (Adh1) of maize."
Gerlach W.L., Pryor A.J., Dennis E.S., Ferl R.J., Sachs M.M., Peacock W.J.
Proc. Natl. Acad. Sci. U.S.A. 79:2981-2985(1982) [PubMed: 16593188] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE OF 212-379.

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Cross-references

Sequence databases

X04049 Genomic DNA. Translation: CAA27681.1.
X00580 mRNA. Translation: CAA25239.1.
X04050 Genomic DNA. Translation: CAA27682.1.
M32984 Genomic DNA. Translation: AAA33434.1.
PIRS04571.
RefSeqNP_001105409.1.
UniGeneZm.1540

3D structure databases

HSSPHSSP built from PDB template 1M6H based on UniProtKB P11766.
ModBaseSearch...

Genome annotation databases

GeneID542363.

Organism-specific databases

GrameneP00333.
MaizeGDB13844.

Enzyme and pathway databases

BRENDA1.1.1.1. 289.

Family and domain databases

InterProIPR013154. ADH_GroES-like.
IPR002085. ADH_SF_Zn.
IPR013149. ADH_Zn-bd.
IPR002328. ADH_Zn_CS.
[Graphical view]
PANTHERPTHR11695. ADH_Sf_Zn. 1 hit.
PfamPF08240. ADH_N. 1 hit.
PF00107. ADH_zinc_N. 1 hit.
[Graphical view]
PROSITEPS00059. ADH_ZINC. 1 hit.
[Graphical view]
ProtoNetSearch...

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Entry information

Entry nameADH1_MAIZE
AccessionPrimary (citable) accession number: P00333
Entry history
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: August 13, 1987
Last modified: June 16, 2009
This is version 81 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectPPAP (Plant Proteome Annotation Project)

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Relevant documents

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents