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Protein

Alcohol dehydrogenase 2

Gene

ADH2

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

This isozyme preferentially catalyzes the conversion of ethanol to acetaldehyde. Acts on a variety of primary unbranched aliphatic alcohols.

Catalytic activityi

An alcohol + NAD+ = an aldehyde or ketone + NADH.

Cofactori

Zn2+By similarityNote: Binds 2 Zn2+ ions per subunit.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi44 – 441Zinc 1; catalytic
Metal bindingi67 – 671Zinc 1; catalytic
Metal bindingi98 – 981Zinc 2
Metal bindingi101 – 1011Zinc 2
Metal bindingi104 – 1041Zinc 2
Metal bindingi112 – 1121Zinc 2
Metal bindingi154 – 1541Zinc 1; catalytic
Binding sitei202 – 2021NADBy similarity
Binding sitei207 – 2071NADBy similarity
Binding sitei341 – 3411NADBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi178 – 1847NADBy similarity
Nucleotide bindingi269 – 2713NADBy similarity

GO - Molecular functioni

  • alcohol dehydrogenase (NAD) activity Source: SGD
  • zinc ion binding Source: InterPro

GO - Biological processi

  • amino acid catabolic process to alcohol via Ehrlich pathway Source: SGD
  • ethanol metabolic process Source: SGD
  • NADH oxidation Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Ligandi

Metal-binding, NAD, Zinc

Enzyme and pathway databases

BioCyciMetaCyc:YMR303C-MONOMER.
YEAST:YMR303C-MONOMER.
BRENDAi1.1.1.1. 984.

Names & Taxonomyi

Protein namesi
Recommended name:
Alcohol dehydrogenase 2 (EC:1.1.1.1)
Alternative name(s):
Alcohol dehydrogenase II
YADH-2
Gene namesi
Name:ADH2
Synonyms:ADR2
Ordered Locus Names:YMR303C
ORF Names:YM9952.05C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XIII

Organism-specific databases

EuPathDBiFungiDB:YMR303C.
SGDiS000004918. ADH2.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemoved1 Publication
Chaini2 – 348347Alcohol dehydrogenase 2PRO_0000160731Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylserine1 Publication
Modified residuei213 – 2131PhosphoserineBy similarity
Modified residuei223 – 2231PhosphothreonineBy similarity
Modified residuei279 – 2791PhosphoserineBy similarity
Modified residuei316 – 3161PhosphoserineBy similarity

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

MaxQBiP00331.
PeptideAtlasiP00331.

PTM databases

iPTMnetiP00331.

Expressioni

Inductioni

Repressed by glucose.

Interactioni

Subunit structurei

Homotetramer.

Protein-protein interaction databases

BioGridi35483. 36 interactions.
DIPiDIP-1181N.
IntActiP00331. 7 interactions.
MINTiMINT-393329.

Structurei

3D structure databases

ProteinModelPortaliP00331.
SMRiP00331. Positions 2-348.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

GeneTreeiENSGT00550000075207.
HOGENOMiHOG000294685.
InParanoidiP00331.
KOiK13953.
OMAiCENGDES.
OrthoDBiEOG7JDR73.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
3.90.180.10. 1 hit.
InterProiIPR013149. ADH_C.
IPR013154. ADH_N.
IPR002085. ADH_SF_Zn-type.
IPR002328. ADH_Zn_CS.
IPR011032. GroES-like.
IPR016040. NAD(P)-bd_dom.
IPR020843. PKS_ER.
[Graphical view]
PANTHERiPTHR11695. PTHR11695. 1 hit.
PfamiPF08240. ADH_N. 1 hit.
PF00107. ADH_zinc_N. 1 hit.
[Graphical view]
SMARTiSM00829. PKS_ER. 1 hit.
[Graphical view]
SUPFAMiSSF50129. SSF50129. 1 hit.
SSF51735. SSF51735. 1 hit.
PROSITEiPS00059. ADH_ZINC. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P00331-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSIPETQKAI IFYESNGKLE HKDIPVPKPK PNELLINVKY SGVCHTDLHA
60 70 80 90 100
WHGDWPLPTK LPLVGGHEGA GVVVGMGENV KGWKIGDYAG IKWLNGSCMA
110 120 130 140 150
CEYCELGNES NCPHADLSGY THDGSFQEYA TADAVQAAHI PQGTDLAEVA
160 170 180 190 200
PILCAGITVY KALKSANLRA GHWAAISGAA GGLGSLAVQY AKAMGYRVLG
210 220 230 240 250
IDGGPGKEEL FTSLGGEVFI DFTKEKDIVS AVVKATNGGA HGIINVSVSE
260 270 280 290 300
AAIEASTRYC RANGTVVLVG LPAGAKCSSD VFNHVVKSIS IVGSYVGNRA
310 320 330 340
DTREALDFFA RGLVKSPIKV VGLSSLPEIY EKMEKGQIAG RYVVDTSK
Length:348
Mass (Da):36,732
Last modified:January 23, 2007 - v3
Checksum:i54535FC3258B10EA
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti16 – 161N → H in AAA34411 (PubMed:6279086).Curated
Sequence conflicti31 – 311P → A in AAA34411 (PubMed:6279086).Curated
Sequence conflicti233 – 2331V → K AA sequence (PubMed:7895733).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J01314 Genomic DNA. Translation: AAA34408.1.
M38457 Genomic DNA. Translation: AAA34411.1.
Z49212 Genomic DNA. Translation: CAA89136.1.
BK006946 Genomic DNA. Translation: DAA10204.1.
PIRiA00340. DEBYA2.
RefSeqiNP_014032.1. NM_001182812.1.

Genome annotation databases

EnsemblFungiiYMR303C; YMR303C; YMR303C.
GeneIDi855349.
KEGGisce:YMR303C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J01314 Genomic DNA. Translation: AAA34408.1.
M38457 Genomic DNA. Translation: AAA34411.1.
Z49212 Genomic DNA. Translation: CAA89136.1.
BK006946 Genomic DNA. Translation: DAA10204.1.
PIRiA00340. DEBYA2.
RefSeqiNP_014032.1. NM_001182812.1.

3D structure databases

ProteinModelPortaliP00331.
SMRiP00331. Positions 2-348.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi35483. 36 interactions.
DIPiDIP-1181N.
IntActiP00331. 7 interactions.
MINTiMINT-393329.

PTM databases

iPTMnetiP00331.

Proteomic databases

MaxQBiP00331.
PeptideAtlasiP00331.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYMR303C; YMR303C; YMR303C.
GeneIDi855349.
KEGGisce:YMR303C.

Organism-specific databases

EuPathDBiFungiDB:YMR303C.
SGDiS000004918. ADH2.

Phylogenomic databases

GeneTreeiENSGT00550000075207.
HOGENOMiHOG000294685.
InParanoidiP00331.
KOiK13953.
OMAiCENGDES.
OrthoDBiEOG7JDR73.

Enzyme and pathway databases

BioCyciMetaCyc:YMR303C-MONOMER.
YEAST:YMR303C-MONOMER.
BRENDAi1.1.1.1. 984.

Miscellaneous databases

PROiP00331.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
3.90.180.10. 1 hit.
InterProiIPR013149. ADH_C.
IPR013154. ADH_N.
IPR002085. ADH_SF_Zn-type.
IPR002328. ADH_Zn_CS.
IPR011032. GroES-like.
IPR016040. NAD(P)-bd_dom.
IPR020843. PKS_ER.
[Graphical view]
PANTHERiPTHR11695. PTHR11695. 1 hit.
PfamiPF08240. ADH_N. 1 hit.
PF00107. ADH_zinc_N. 1 hit.
[Graphical view]
SMARTiSM00829. PKS_ER. 1 hit.
[Graphical view]
SUPFAMiSSF50129. SSF50129. 1 hit.
SSF51735. SSF51735. 1 hit.
PROSITEiPS00059. ADH_ZINC. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Nucleotide sequence of the yeast alcohol dehydrogenase II gene."
    Russell D.W., Smith M., Williamson V.M., Young E.T.
    J. Biol. Chem. 258:2674-2682(1983) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "The alcohol dehydrogenase genes of the yeast, Saccharomyces cerevisiae: isolation, structure, and regulation."
    Young E.T., Williamson V.M., Taguchi A., Smith M., Sledziewski A., Russell D.W., Osterman J., Denis C., Cox D., Beier D.
    Basic Life Sci. 19:335-361(1982) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  4. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  5. Cited for: PROTEIN SEQUENCE OF 193-207 AND 227-234.
    Strain: ATCC 204508 / S288c.
  6. "Proteome studies of Saccharomyces cerevisiae: identification and characterization of abundant proteins."
    Garrels J.I., McLaughlin C.S., Warner J.R., Futcher B., Latter G.I., Kobayashi R., Schwender B., Volpe T., Anderson D.S., Mesquita-Fuentes R., Payne W.E.
    Electrophoresis 18:1347-1360(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: ACETYLATION AT SER-2.
  7. "The three zinc-containing alcohol dehydrogenases from baker's yeast, Saccharomyces cerevisiae."
    Leskovac V., Trivic S., Pericin D.
    FEMS Yeast Res. 2:481-494(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: REVIEW.
  8. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiADH2_YEAST
AccessioniPrimary (citable) accession number: P00331
Secondary accession number(s): D6W0D0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: January 23, 2007
Last modified: June 8, 2016
This is version 162 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 1620 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XIII
    Yeast (Saccharomyces cerevisiae) chromosome XIII: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.