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Protein

Alcohol dehydrogenase 2

Gene

ADH2

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

This isozyme preferentially catalyzes the conversion of ethanol to acetaldehyde. Acts on a variety of primary unbranched aliphatic alcohols.

Catalytic activityi

An alcohol + NAD+ = an aldehyde or ketone + NADH.

Cofactori

Zn2+By similarityNote: Binds 2 Zn2+ ions per subunit.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi44Zinc 1; catalytic1
Metal bindingi67Zinc 1; catalytic1
Metal bindingi98Zinc 21
Metal bindingi101Zinc 21
Metal bindingi104Zinc 21
Metal bindingi112Zinc 21
Metal bindingi154Zinc 1; catalytic1
Binding sitei202NADBy similarity1
Binding sitei207NADBy similarity1
Binding sitei341NADBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi178 – 184NADBy similarity7
Nucleotide bindingi269 – 271NADBy similarity3

GO - Molecular functioni

  • alcohol dehydrogenase (NAD) activity Source: SGD
  • zinc ion binding Source: InterPro

GO - Biological processi

  • amino acid catabolic process to alcohol via Ehrlich pathway Source: SGD
  • ethanol metabolic process Source: SGD
  • NADH oxidation Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Ligandi

Metal-binding, NAD, Zinc

Enzyme and pathway databases

BioCyciMetaCyc:YMR303C-MONOMER.
YEAST:YMR303C-MONOMER.
BRENDAi1.1.1.1. 984.

Names & Taxonomyi

Protein namesi
Recommended name:
Alcohol dehydrogenase 2 (EC:1.1.1.1)
Alternative name(s):
Alcohol dehydrogenase II
YADH-2
Gene namesi
Name:ADH2
Synonyms:ADR2
Ordered Locus Names:YMR303C
ORF Names:YM9952.05C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XIII

Organism-specific databases

EuPathDBiFungiDB:YMR303C.
SGDiS000004918. ADH2.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved1 Publication
ChainiPRO_00001607312 – 348Alcohol dehydrogenase 2Add BLAST347

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylserine1 Publication1
Modified residuei213PhosphoserineBy similarity1
Modified residuei223PhosphothreonineBy similarity1
Cross-linki226Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)By similarity
Cross-linki234Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)By similarity
Modified residuei279PhosphoserineBy similarity1
Cross-linki287Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)By similarity
Modified residuei316PhosphoserineBy similarity1
Cross-linki319Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)By similarity

Keywords - PTMi

Acetylation, Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

MaxQBiP00331.
PRIDEiP00331.

PTM databases

iPTMnetiP00331.

Expressioni

Inductioni

Repressed by glucose.

Interactioni

Subunit structurei

Homotetramer.

Protein-protein interaction databases

BioGridi35483. 36 interactors.
DIPiDIP-1181N.
IntActiP00331. 7 interactors.
MINTiMINT-393329.

Structurei

3D structure databases

ProteinModelPortaliP00331.
SMRiP00331.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

GeneTreeiENSGT00550000075207.
HOGENOMiHOG000294685.
InParanoidiP00331.
KOiK13953.
OMAiGYYPRMK.
OrthoDBiEOG092C2Q8E.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
3.90.180.10. 1 hit.
InterProiIPR013149. ADH_C.
IPR013154. ADH_N.
IPR002085. ADH_SF_Zn-type.
IPR002328. ADH_Zn_CS.
IPR011032. GroES-like.
IPR016040. NAD(P)-bd_dom.
IPR020843. PKS_ER.
[Graphical view]
PANTHERiPTHR11695. PTHR11695. 1 hit.
PfamiPF08240. ADH_N. 1 hit.
PF00107. ADH_zinc_N. 1 hit.
[Graphical view]
SMARTiSM00829. PKS_ER. 1 hit.
[Graphical view]
SUPFAMiSSF50129. SSF50129. 1 hit.
SSF51735. SSF51735. 1 hit.
PROSITEiPS00059. ADH_ZINC. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P00331-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSIPETQKAI IFYESNGKLE HKDIPVPKPK PNELLINVKY SGVCHTDLHA
60 70 80 90 100
WHGDWPLPTK LPLVGGHEGA GVVVGMGENV KGWKIGDYAG IKWLNGSCMA
110 120 130 140 150
CEYCELGNES NCPHADLSGY THDGSFQEYA TADAVQAAHI PQGTDLAEVA
160 170 180 190 200
PILCAGITVY KALKSANLRA GHWAAISGAA GGLGSLAVQY AKAMGYRVLG
210 220 230 240 250
IDGGPGKEEL FTSLGGEVFI DFTKEKDIVS AVVKATNGGA HGIINVSVSE
260 270 280 290 300
AAIEASTRYC RANGTVVLVG LPAGAKCSSD VFNHVVKSIS IVGSYVGNRA
310 320 330 340
DTREALDFFA RGLVKSPIKV VGLSSLPEIY EKMEKGQIAG RYVVDTSK
Length:348
Mass (Da):36,732
Last modified:January 23, 2007 - v3
Checksum:i54535FC3258B10EA
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti16N → H in AAA34411 (PubMed:6279086).Curated1
Sequence conflicti31P → A in AAA34411 (PubMed:6279086).Curated1
Sequence conflicti233V → K AA sequence (PubMed:7895733).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J01314 Genomic DNA. Translation: AAA34408.1.
M38457 Genomic DNA. Translation: AAA34411.1.
Z49212 Genomic DNA. Translation: CAA89136.1.
BK006946 Genomic DNA. Translation: DAA10204.1.
PIRiA00340. DEBYA2.
RefSeqiNP_014032.1. NM_001182812.1.

Genome annotation databases

EnsemblFungiiYMR303C; YMR303C; YMR303C.
GeneIDi855349.
KEGGisce:YMR303C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J01314 Genomic DNA. Translation: AAA34408.1.
M38457 Genomic DNA. Translation: AAA34411.1.
Z49212 Genomic DNA. Translation: CAA89136.1.
BK006946 Genomic DNA. Translation: DAA10204.1.
PIRiA00340. DEBYA2.
RefSeqiNP_014032.1. NM_001182812.1.

3D structure databases

ProteinModelPortaliP00331.
SMRiP00331.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi35483. 36 interactors.
DIPiDIP-1181N.
IntActiP00331. 7 interactors.
MINTiMINT-393329.

PTM databases

iPTMnetiP00331.

Proteomic databases

MaxQBiP00331.
PRIDEiP00331.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYMR303C; YMR303C; YMR303C.
GeneIDi855349.
KEGGisce:YMR303C.

Organism-specific databases

EuPathDBiFungiDB:YMR303C.
SGDiS000004918. ADH2.

Phylogenomic databases

GeneTreeiENSGT00550000075207.
HOGENOMiHOG000294685.
InParanoidiP00331.
KOiK13953.
OMAiGYYPRMK.
OrthoDBiEOG092C2Q8E.

Enzyme and pathway databases

BioCyciMetaCyc:YMR303C-MONOMER.
YEAST:YMR303C-MONOMER.
BRENDAi1.1.1.1. 984.

Miscellaneous databases

PROiP00331.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
3.90.180.10. 1 hit.
InterProiIPR013149. ADH_C.
IPR013154. ADH_N.
IPR002085. ADH_SF_Zn-type.
IPR002328. ADH_Zn_CS.
IPR011032. GroES-like.
IPR016040. NAD(P)-bd_dom.
IPR020843. PKS_ER.
[Graphical view]
PANTHERiPTHR11695. PTHR11695. 1 hit.
PfamiPF08240. ADH_N. 1 hit.
PF00107. ADH_zinc_N. 1 hit.
[Graphical view]
SMARTiSM00829. PKS_ER. 1 hit.
[Graphical view]
SUPFAMiSSF50129. SSF50129. 1 hit.
SSF51735. SSF51735. 1 hit.
PROSITEiPS00059. ADH_ZINC. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiADH2_YEAST
AccessioniPrimary (citable) accession number: P00331
Secondary accession number(s): D6W0D0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: January 23, 2007
Last modified: November 2, 2016
This is version 166 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 1620 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XIII
    Yeast (Saccharomyces cerevisiae) chromosome XIII: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.