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Protein

Alcohol dehydrogenase 1

Gene

ADH1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

This isozyme preferentially catalyzes the conversion of primary unbranched alcohols to their corresponding aldehydes. Also also shows activity toward secondary alcohols.

Catalytic activityi

An alcohol + NAD+ = an aldehyde or ketone + NADH.

Cofactori

Zn2+Note: Binds 2 Zn2+ ions per subunit.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi44Zinc 1; catalytic1
Metal bindingi67Zinc 1; catalytic1
Metal bindingi98Zinc 21
Metal bindingi101Zinc 21
Metal bindingi104Zinc 21
Metal bindingi112Zinc 21
Metal bindingi154Zinc 1; catalytic1
Binding sitei202NADBy similarity1
Binding sitei207NADBy similarity1
Binding sitei341NADBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi178 – 184NADBy similarity7
Nucleotide bindingi269 – 271NADBy similarity3

GO - Molecular functioni

  • alcohol dehydrogenase (NAD) activity Source: SGD
  • methylglyoxal reductase (NADH-dependent) activity Source: SGD
  • zinc ion binding Source: InterPro

GO - Biological processi

  • amino acid catabolic process to alcohol via Ehrlich pathway Source: SGD
  • ethanol biosynthetic process involved in glucose fermentation to ethanol Source: SGD
  • NADH oxidation Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Ligandi

Metal-binding, NAD, Zinc

Enzyme and pathway databases

BioCyciMetaCyc:YOL086C-MONOMER.
YEAST:YOL086C-MONOMER.
BRENDAi1.1.1.1. 984.

Names & Taxonomyi

Protein namesi
Recommended name:
Alcohol dehydrogenase 1 (EC:1.1.1.1)
Alternative name(s):
Alcohol dehydrogenase I
YADH-1
Gene namesi
Name:ADH1
Synonyms:ADC1
Ordered Locus Names:YOL086C
ORF Names:O0947
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XV

Organism-specific databases

EuPathDBiFungiDB:YOL086C.
SGDiS000005446. ADH1.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved1 Publication
ChainiPRO_00001607302 – 348Alcohol dehydrogenase 1Add BLAST347

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylserine1 Publication1
Modified residuei213PhosphoserineCombined sources1
Modified residuei223PhosphothreonineCombined sources1
Cross-linki226Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)Combined sources
Cross-linki234Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)Combined sources
Modified residuei279PhosphoserineCombined sources1
Cross-linki287Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)Combined sources
Modified residuei316PhosphoserineCombined sources1
Cross-linki319Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)Combined sources

Keywords - PTMi

Acetylation, Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

MaxQBiP00330.
PRIDEiP00330.
TopDownProteomicsiP00330.

2D gel databases

COMPLUYEAST-2DPAGEP00330.
SWISS-2DPAGEP00330.

PTM databases

iPTMnetiP00330.
SwissPalmiP00330.

Interactioni

Subunit structurei

Homotetramer.

Protein-protein interaction databases

BioGridi34317. 141 interactors.
DIPiDIP-1143N.
IntActiP00330. 213 interactors.
MINTiMINT-642403.

Structurei

Secondary structure

1348
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi5 – 14Combined sources10
Beta strandi20 – 25Combined sources6
Beta strandi33 – 42Combined sources10
Helixi45 – 52Combined sources8
Beta strandi55 – 57Combined sources3
Beta strandi61 – 64Combined sources4
Beta strandi68 – 76Combined sources9
Beta strandi88 – 91Combined sources4
Beta strandi93 – 96Combined sources4
Beta strandi99 – 101Combined sources3
Helixi102 – 105Combined sources4
Helixi109 – 111Combined sources3
Beta strandi116 – 118Combined sources3
Turni119 – 121Combined sources3
Beta strandi125 – 133Combined sources9
Turni134 – 136Combined sources3
Beta strandi137 – 140Combined sources4
Helixi146 – 149Combined sources4
Helixi150 – 153Combined sources4
Helixi155 – 164Combined sources10
Beta strandi173 – 177Combined sources5
Turni178 – 180Combined sources3
Helixi184 – 194Combined sources11
Beta strandi197 – 202Combined sources6
Helixi207 – 213Combined sources7
Beta strandi218 – 221Combined sources4
Turni222 – 224Combined sources3
Helixi228 – 235Combined sources8
Beta strandi240 – 245Combined sources6
Helixi250 – 259Combined sources10
Beta strandi260 – 268Combined sources9
Beta strandi276 – 280Combined sources5
Helixi281 – 286Combined sources6
Beta strandi290 – 293Combined sources4
Helixi299 – 310Combined sources12
Beta strandi318 – 322Combined sources5
Helixi323 – 325Combined sources3
Helixi326 – 334Combined sources9
Beta strandi339 – 346Combined sources8

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4W6ZX-ray2.40A/B/C/D2-348[»]
5ENVX-ray3.00A/B2-348[»]
ProteinModelPortaliP00330.
SMRiP00330.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP00330.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

GeneTreeiENSGT00550000075207.
HOGENOMiHOG000294685.
InParanoidiP00330.
KOiK13953.
OMAiRVATCGA.
OrthoDBiEOG092C2Q8E.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
3.90.180.10. 1 hit.
InterProiIPR013149. ADH_C.
IPR013154. ADH_N.
IPR002085. ADH_SF_Zn-type.
IPR002328. ADH_Zn_CS.
IPR011032. GroES-like.
IPR016040. NAD(P)-bd_dom.
IPR020843. PKS_ER.
[Graphical view]
PANTHERiPTHR11695. PTHR11695. 1 hit.
PfamiPF08240. ADH_N. 1 hit.
PF00107. ADH_zinc_N. 1 hit.
[Graphical view]
SMARTiSM00829. PKS_ER. 1 hit.
[Graphical view]
SUPFAMiSSF50129. SSF50129. 1 hit.
SSF51735. SSF51735. 1 hit.
PROSITEiPS00059. ADH_ZINC. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P00330-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSIPETQKGV IFYESHGKLE YKDIPVPKPK ANELLINVKY SGVCHTDLHA
60 70 80 90 100
WHGDWPLPVK LPLVGGHEGA GVVVGMGENV KGWKIGDYAG IKWLNGSCMA
110 120 130 140 150
CEYCELGNES NCPHADLSGY THDGSFQQYA TADAVQAAHI PQGTDLAQVA
160 170 180 190 200
PILCAGITVY KALKSANLMA GHWVAISGAA GGLGSLAVQY AKAMGYRVLG
210 220 230 240 250
IDGGEGKEEL FRSIGGEVFI DFTKEKDIVG AVLKATDGGA HGVINVSVSE
260 270 280 290 300
AAIEASTRYV RANGTTVLVG MPAGAKCCSD VFNQVVKSIS IVGSYVGNRA
310 320 330 340
DTREALDFFA RGLVKSPIKV VGLSTLPEIY EKMEKGQIVG RYVVDTSK
Length:348
Mass (Da):36,849
Last modified:October 5, 2010 - v5
Checksum:iF14AE24B5D8D12A5
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti21Y → H in CAA24601 (PubMed:6277922).Curated1
Sequence conflicti21Y → H in AAA34410 (PubMed:6279086).Curated1
Sequence conflicti59V → T AA sequence (PubMed:320000).Curated1
Sequence conflicti148Q → E AA sequence (PubMed:320000).Curated1
Sequence conflicti152I → V AA sequence (PubMed:320000).Curated1
Sequence conflicti237D → N AA sequence (PubMed:320000).Curated1
Sequence conflicti314V → I AA sequence (PubMed:320000).Curated1
Sequence conflicti338I → V AA sequence (PubMed:320000).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural varianti236T → I.1 Publication1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
V01292 Genomic DNA. Translation: CAA24601.1.
M38456 Genomic DNA. Translation: AAA34410.1.
X83121 Genomic DNA. Translation: CAA58193.1.
Z74828 Genomic DNA. Translation: CAA99098.1.
V01291 Genomic DNA. Translation: CAA24600.1.
BK006948 Genomic DNA. Translation: DAA10699.1.
PIRiS57383. DEBYA.
RefSeqiNP_014555.1. NM_001183340.1.

Genome annotation databases

EnsemblFungiiYOL086C; YOL086C; YOL086C.
GeneIDi854068.
KEGGisce:YOL086C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
V01292 Genomic DNA. Translation: CAA24601.1.
M38456 Genomic DNA. Translation: AAA34410.1.
X83121 Genomic DNA. Translation: CAA58193.1.
Z74828 Genomic DNA. Translation: CAA99098.1.
V01291 Genomic DNA. Translation: CAA24600.1.
BK006948 Genomic DNA. Translation: DAA10699.1.
PIRiS57383. DEBYA.
RefSeqiNP_014555.1. NM_001183340.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4W6ZX-ray2.40A/B/C/D2-348[»]
5ENVX-ray3.00A/B2-348[»]
ProteinModelPortaliP00330.
SMRiP00330.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi34317. 141 interactors.
DIPiDIP-1143N.
IntActiP00330. 213 interactors.
MINTiMINT-642403.

PTM databases

iPTMnetiP00330.
SwissPalmiP00330.

2D gel databases

COMPLUYEAST-2DPAGEP00330.
SWISS-2DPAGEP00330.

Proteomic databases

MaxQBiP00330.
PRIDEiP00330.
TopDownProteomicsiP00330.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYOL086C; YOL086C; YOL086C.
GeneIDi854068.
KEGGisce:YOL086C.

Organism-specific databases

EuPathDBiFungiDB:YOL086C.
SGDiS000005446. ADH1.

Phylogenomic databases

GeneTreeiENSGT00550000075207.
HOGENOMiHOG000294685.
InParanoidiP00330.
KOiK13953.
OMAiRVATCGA.
OrthoDBiEOG092C2Q8E.

Enzyme and pathway databases

BioCyciMetaCyc:YOL086C-MONOMER.
YEAST:YOL086C-MONOMER.
BRENDAi1.1.1.1. 984.

Miscellaneous databases

EvolutionaryTraceiP00330.
PROiP00330.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
3.90.180.10. 1 hit.
InterProiIPR013149. ADH_C.
IPR013154. ADH_N.
IPR002085. ADH_SF_Zn-type.
IPR002328. ADH_Zn_CS.
IPR011032. GroES-like.
IPR016040. NAD(P)-bd_dom.
IPR020843. PKS_ER.
[Graphical view]
PANTHERiPTHR11695. PTHR11695. 1 hit.
PfamiPF08240. ADH_N. 1 hit.
PF00107. ADH_zinc_N. 1 hit.
[Graphical view]
SMARTiSM00829. PKS_ER. 1 hit.
[Graphical view]
SUPFAMiSSF50129. SSF50129. 1 hit.
SSF51735. SSF51735. 1 hit.
PROSITEiPS00059. ADH_ZINC. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiADH1_YEAST
AccessioniPrimary (citable) accession number: P00330
Secondary accession number(s): D6W1Y3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: October 5, 2010
Last modified: November 2, 2016
This is version 180 of the entry and version 5 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

PubMed:320000 sequence has several conflicting residues and reports microheterogeneities at additional postitions. Analysis of the sequence suggests that the sequenced protein was a mixture of at least 3 of the different isoforms of alcohol dehydrogenases found in yeast.

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome XV
    Yeast (Saccharomyces cerevisiae) chromosome XV: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.