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P00329 (ADH1_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 137. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Alcohol dehydrogenase 1

EC=1.1.1.1
Alternative name(s):
ADH-A2
Alcohol dehydrogenase A subunit
Gene names
Name:Adh1
Synonyms:Adh-1
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length375 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Catalytic activity

An alcohol + NAD+ = an aldehyde or ketone + NADH.

Cofactor

Binds 2 zinc ions per subunit.

Subunit structure

Dimer of identical or non-identical chains of three types (A, B, C), which are coded by 3 separate genes at different loci.

Subcellular location

Cytoplasm.

Tissue specificity

Expressed at high levels in the liver, small intestine and eye, at moderate levels in kidney, ovary and uterus, and at low levels in the spinal cord, thymus, heart, stomach mucosa, skin and testis. Ref.7

Sequence similarities

Belongs to the zinc-containing alcohol dehydrogenase family. Class-I subfamily.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed By similarity
Chain2 – 375374Alcohol dehydrogenase 1
PRO_0000160666

Regions

Nucleotide binding200 – 2056NAD By similarity
Nucleotide binding293 – 2953NAD By similarity

Sites

Metal binding471Zinc 1; catalytic
Metal binding681Zinc 1; catalytic
Metal binding981Zinc 2
Metal binding1011Zinc 2
Metal binding1041Zinc 2
Metal binding1121Zinc 2
Metal binding1751Zinc 1; catalytic
Binding site2241NAD By similarity
Binding site2291NAD By similarity
Binding site3701NAD By similarity

Amino acid modifications

Modified residue21N-acetylserine By similarity
Modified residue2341N6-succinyllysine Ref.8
Modified residue3401N6-succinyllysine Ref.8

Sequences

Sequence LengthMass (Da)Tools
P00329 [UniParc].

Last modified January 23, 2007. Version 2.
Checksum: 7B8AF94C95D35108

FASTA37539,771
        10         20         30         40         50         60 
MSTAGKVIKC KAAVLWELHK PFTIEDIEVA PPKAHEVRIK MVATGVCRSD DHVVSGTLVT 

        70         80         90        100        110        120 
PLPAVLGHEG AGIVESVGEG VTCVKPGDKV IPLFSPQCGE CRICKHPESN FCSRSDLLMP 

       130        140        150        160        170        180 
RGTLREGTSR FSCKGKQIHN FISTSTFSQY TVVDDIAVAK IDGASPLDKV CLIGCGFSTG 

       190        200        210        220        230        240 
YGSAVKVAKV TPGSTCAVFG LGGVGLSVII GCKAAGAARI IAVDINKDKF AKAKELGATE 

       250        260        270        280        290        300 
CINPQDYSKP IQEVLQEMTD GGVDFSFEVI GRLDTMTSAL LSCHAACGVS VVVGVPPNAQ 

       310        320        330        340        350        360 
NLSMNPMLLL LGRTWKGAIF GGFKSKDSVP KLVADFMAKK FPLDPLITHV LPFEKINEAF 

       370 
DLLRSGKSIR TVLTF 

« Hide

References

« Hide 'large scale' references
[1]"Cloning and sequencing of cDNA encoding the complete mouse liver alcohol dehydrogenase."
Edenberg H.J., Zhang K., Fong K., Bosron W.F., Li T.-K.
Proc. Natl. Acad. Sci. U.S.A. 82:2262-2266(1985) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]"Molecular analysis of mouse alcohol dehydrogenase: nucleotide sequence of the Adh-1 gene and genetic mapping of a related nucleotide sequence to chromosome 3."
Ceci J.D., Zheng Y.W., Felder M.R.
Gene 59:171-182(1987) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]"Structure of the mouse Adh-1 gene and identification of a deletion in a long alternating purine-pyrimidine sequence in the first intron of strains expressing low alcohol dehydrogenase activity."
Zhang K., Bosron W.F., Edenberg H.J.
Gene 57:27-36(1987) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[4]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: FVB/N.
Tissue: Colon and Kidney.
[5]"Isolation of genomic DNA fragments corresponding to genes modulated in vivo by a transcription factor."
Caubin J., Iglesias T., Bernal J., Munoz A., Marquez G., Barbero J.L., Zaballos A.
Nucleic Acids Res. 22:4132-4138(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 8-51.
[6]"Androgen induction of alcohol dehydrogenase in mouse kidney. Studies with a cDNA probe confirmed by nucleotide sequence analysis."
Ceci J.D., Lawther R., Duester G., Hatfield G.W., Smith M., O'Malley M.P., Felder M.R.
Gene 41:217-224(1986) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 224-375.
Strain: SWR/J.
Tissue: Liver.
[7]"Cloning of the mouse class IV alcohol dehydrogenase (retinol dehydrogenase) cDNA and tissue-specific expression patterns of the murine ADH gene family."
Zgombic-Knight M., Ang H.L., Foglio M.H., Duester G.
J. Biol. Chem. 270:10868-10877(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: TISSUE SPECIFICITY.
Strain: FVB/N.
[8]"SIRT5-mediated lysine desuccinylation impacts diverse metabolic pathways."
Park J., Chen Y., Tishkoff D.X., Peng C., Tan M., Dai L., Xie Z., Zhang Y., Zwaans B.M., Skinner M.E., Lombard D.B., Zhao Y.
Mol. Cell 50:919-930(2013) [PubMed] [Europe PMC] [Abstract]
Cited for: SUCCINYLATION [LARGE SCALE ANALYSIS] AT LYS-234 AND LYS-340, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Liver.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M18480 expand/collapse EMBL AC list , M18472, M18473, M18474, M18475, M18476, M18477, M18478 Genomic DNA. Translation: AAA37178.1.
M22679 expand/collapse EMBL AC list , M22671, M22672, M22673, M22674, M22675, M22676, M22677 Genomic DNA. Translation: AAA37179.1.
M11307 mRNA. Translation: AAA37180.1.
BC013477 mRNA. Translation: AAH13477.1.
BC054467 mRNA. Translation: AAH54467.1.
Z32540 Genomic DNA. No translation available.
M22611 mRNA. Translation: AAA37181.1.
CCDSCCDS17867.1.
PIRDEMSAA. A27322.
RefSeqNP_031435.1. NM_007409.2.
UniGeneMm.2409.
Mm.412004.

3D structure databases

ProteinModelPortalP00329.
SMRP00329. Positions 2-375.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

IntActP00329. 4 interactions.
MINTMINT-1869642.
STRING10090.ENSMUSP00000004232.

PTM databases

PhosphoSiteP00329.

Proteomic databases

MaxQBP00329.
PaxDbP00329.
PRIDEP00329.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000004232; ENSMUSP00000004232; ENSMUSG00000074207.
GeneID11522.
KEGGmmu:11522.
UCSCuc008rnf.1. mouse.

Organism-specific databases

CTD11522.
MGIMGI:87921. Adh1.

Phylogenomic databases

eggNOGCOG1062.
HOGENOMHOG000294674.
HOVERGENHBG000195.
InParanoidP00329.
KOK13951.
OMAWGSATHI.
OrthoDBEOG72NRQ6.
PhylomeDBP00329.
TreeFamTF300429.

Enzyme and pathway databases

SABIO-RKP00329.

Gene expression databases

ArrayExpressP00329.
BgeeP00329.
CleanExMM_ADH1.
GenevestigatorP00329.

Family and domain databases

Gene3D3.40.50.720. 1 hit.
3.90.180.10. 1 hit.
InterProIPR013149. ADH_C.
IPR013154. ADH_GroES-like.
IPR002085. ADH_SF_Zn-type.
IPR002328. ADH_Zn_CS.
IPR011032. GroES-like.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PANTHERPTHR11695. PTHR11695. 1 hit.
PfamPF08240. ADH_N. 1 hit.
PF00107. ADH_zinc_N. 1 hit.
[Graphical view]
SUPFAMSSF50129. SSF50129. 2 hits.
PROSITEPS00059. ADH_ZINC. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio278960.
PROP00329.
SOURCESearch...

Entry information

Entry nameADH1_MOUSE
AccessionPrimary (citable) accession number: P00329
Entry history
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: January 23, 2007
Last modified: July 9, 2014
This is version 137 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot