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P00329

- ADH1_MOUSE

UniProt

P00329 - ADH1_MOUSE

Protein

Alcohol dehydrogenase 1

Gene

Adh1

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 139 (01 Oct 2014)
      Sequence version 2 (23 Jan 2007)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    An alcohol + NAD+ = an aldehyde or ketone + NADH.

    Cofactori

    Binds 2 zinc ions per subunit.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi47 – 471Zinc 1; catalytic
    Metal bindingi68 – 681Zinc 1; catalytic
    Metal bindingi98 – 981Zinc 2
    Metal bindingi101 – 1011Zinc 2
    Metal bindingi104 – 1041Zinc 2
    Metal bindingi112 – 1121Zinc 2
    Metal bindingi175 – 1751Zinc 1; catalytic
    Binding sitei224 – 2241NADBy similarity
    Binding sitei229 – 2291NADBy similarity
    Binding sitei370 – 3701NADBy similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi200 – 2056NADBy similarity
    Nucleotide bindingi293 – 2953NADBy similarity

    GO - Molecular functioni

    1. alcohol dehydrogenase (NAD) activity Source: MGI
    2. protein homodimerization activity Source: MGI
    3. zinc ion binding Source: InterPro

    GO - Biological processi

    1. behavioral response to ethanol Source: MGI
    2. ethanol catabolic process Source: MGI
    3. response to retinoic acid Source: MGI
    4. response to steroid hormone Source: MGI
    5. response to testosterone Source: MGI
    6. retinoic acid metabolic process Source: MGI
    7. retinoid metabolic process Source: MGI
    8. retinol metabolic process Source: MGI

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Ligandi

    Metal-binding, NAD, Zinc

    Enzyme and pathway databases

    ReactomeiREACT_196553. Abacavir metabolism.
    SABIO-RKP00329.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Alcohol dehydrogenase 1 (EC:1.1.1.1)
    Alternative name(s):
    ADH-A2
    Alcohol dehydrogenase A subunit
    Gene namesi
    Name:Adh1
    Synonyms:Adh-1
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 3

    Organism-specific databases

    MGIiMGI:87921. Adh1.

    Subcellular locationi

    GO - Cellular componenti

    1. intracellular Source: MGI
    2. mitochondrion Source: MGI

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11RemovedBy similarity
    Chaini2 – 375374Alcohol dehydrogenase 1PRO_0000160666Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei2 – 21N-acetylserineBy similarity
    Modified residuei234 – 2341N6-succinyllysine1 Publication
    Modified residuei340 – 3401N6-succinyllysine1 Publication

    Keywords - PTMi

    Acetylation

    Proteomic databases

    MaxQBiP00329.
    PaxDbiP00329.
    PRIDEiP00329.

    PTM databases

    PhosphoSiteiP00329.

    Expressioni

    Tissue specificityi

    Expressed at high levels in the liver, small intestine and eye, at moderate levels in kidney, ovary and uterus, and at low levels in the spinal cord, thymus, heart, stomach mucosa, skin and testis.1 Publication

    Gene expression databases

    ArrayExpressiP00329.
    BgeeiP00329.
    CleanExiMM_ADH1.
    GenevestigatoriP00329.

    Interactioni

    Subunit structurei

    Dimer of identical or non-identical chains of three types (A, B, C), which are coded by 3 separate genes at different loci.

    Protein-protein interaction databases

    IntActiP00329. 4 interactions.
    MINTiMINT-1869642.
    STRINGi10090.ENSMUSP00000004232.

    Structurei

    3D structure databases

    ProteinModelPortaliP00329.
    SMRiP00329. Positions 2-375.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiCOG1062.
    HOGENOMiHOG000294674.
    HOVERGENiHBG000195.
    InParanoidiP00329.
    KOiK13951.
    OMAiWGSATHI.
    OrthoDBiEOG72NRQ6.
    PhylomeDBiP00329.
    TreeFamiTF300429.

    Family and domain databases

    Gene3Di3.40.50.720. 1 hit.
    3.90.180.10. 1 hit.
    InterProiIPR013149. ADH_C.
    IPR013154. ADH_GroES-like.
    IPR002085. ADH_SF_Zn-type.
    IPR002328. ADH_Zn_CS.
    IPR011032. GroES-like.
    IPR016040. NAD(P)-bd_dom.
    [Graphical view]
    PANTHERiPTHR11695. PTHR11695. 1 hit.
    PfamiPF08240. ADH_N. 1 hit.
    PF00107. ADH_zinc_N. 1 hit.
    [Graphical view]
    SUPFAMiSSF50129. SSF50129. 2 hits.
    PROSITEiPS00059. ADH_ZINC. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P00329-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSTAGKVIKC KAAVLWELHK PFTIEDIEVA PPKAHEVRIK MVATGVCRSD    50
    DHVVSGTLVT PLPAVLGHEG AGIVESVGEG VTCVKPGDKV IPLFSPQCGE 100
    CRICKHPESN FCSRSDLLMP RGTLREGTSR FSCKGKQIHN FISTSTFSQY 150
    TVVDDIAVAK IDGASPLDKV CLIGCGFSTG YGSAVKVAKV TPGSTCAVFG 200
    LGGVGLSVII GCKAAGAARI IAVDINKDKF AKAKELGATE CINPQDYSKP 250
    IQEVLQEMTD GGVDFSFEVI GRLDTMTSAL LSCHAACGVS VVVGVPPNAQ 300
    NLSMNPMLLL LGRTWKGAIF GGFKSKDSVP KLVADFMAKK FPLDPLITHV 350
    LPFEKINEAF DLLRSGKSIR TVLTF 375
    Length:375
    Mass (Da):39,771
    Last modified:January 23, 2007 - v2
    Checksum:i7B8AF94C95D35108
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M18480
    , M18472, M18473, M18474, M18475, M18476, M18477, M18478 Genomic DNA. Translation: AAA37178.1.
    M22679
    , M22671, M22672, M22673, M22674, M22675, M22676, M22677 Genomic DNA. Translation: AAA37179.1.
    M11307 mRNA. Translation: AAA37180.1.
    BC013477 mRNA. Translation: AAH13477.1.
    BC054467 mRNA. Translation: AAH54467.1.
    Z32540 Genomic DNA. No translation available.
    M22611 mRNA. Translation: AAA37181.1.
    CCDSiCCDS17867.1.
    PIRiA27322. DEMSAA.
    RefSeqiNP_031435.1. NM_007409.2.
    UniGeneiMm.2409.
    Mm.412004.

    Genome annotation databases

    EnsembliENSMUST00000004232; ENSMUSP00000004232; ENSMUSG00000074207.
    GeneIDi11522.
    KEGGimmu:11522.
    UCSCiuc008rnf.1. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M18480
    , M18472 , M18473 , M18474 , M18475 , M18476 , M18477 , M18478 Genomic DNA. Translation: AAA37178.1 .
    M22679
    , M22671 , M22672 , M22673 , M22674 , M22675 , M22676 , M22677 Genomic DNA. Translation: AAA37179.1 .
    M11307 mRNA. Translation: AAA37180.1 .
    BC013477 mRNA. Translation: AAH13477.1 .
    BC054467 mRNA. Translation: AAH54467.1 .
    Z32540 Genomic DNA. No translation available.
    M22611 mRNA. Translation: AAA37181.1 .
    CCDSi CCDS17867.1.
    PIRi A27322. DEMSAA.
    RefSeqi NP_031435.1. NM_007409.2.
    UniGenei Mm.2409.
    Mm.412004.

    3D structure databases

    ProteinModelPortali P00329.
    SMRi P00329. Positions 2-375.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi P00329. 4 interactions.
    MINTi MINT-1869642.
    STRINGi 10090.ENSMUSP00000004232.

    PTM databases

    PhosphoSitei P00329.

    Proteomic databases

    MaxQBi P00329.
    PaxDbi P00329.
    PRIDEi P00329.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000004232 ; ENSMUSP00000004232 ; ENSMUSG00000074207 .
    GeneIDi 11522.
    KEGGi mmu:11522.
    UCSCi uc008rnf.1. mouse.

    Organism-specific databases

    CTDi 11522.
    MGIi MGI:87921. Adh1.

    Phylogenomic databases

    eggNOGi COG1062.
    HOGENOMi HOG000294674.
    HOVERGENi HBG000195.
    InParanoidi P00329.
    KOi K13951.
    OMAi WGSATHI.
    OrthoDBi EOG72NRQ6.
    PhylomeDBi P00329.
    TreeFami TF300429.

    Enzyme and pathway databases

    Reactomei REACT_196553. Abacavir metabolism.
    SABIO-RK P00329.

    Miscellaneous databases

    NextBioi 278960.
    PROi P00329.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi P00329.
    Bgeei P00329.
    CleanExi MM_ADH1.
    Genevestigatori P00329.

    Family and domain databases

    Gene3Di 3.40.50.720. 1 hit.
    3.90.180.10. 1 hit.
    InterProi IPR013149. ADH_C.
    IPR013154. ADH_GroES-like.
    IPR002085. ADH_SF_Zn-type.
    IPR002328. ADH_Zn_CS.
    IPR011032. GroES-like.
    IPR016040. NAD(P)-bd_dom.
    [Graphical view ]
    PANTHERi PTHR11695. PTHR11695. 1 hit.
    Pfami PF08240. ADH_N. 1 hit.
    PF00107. ADH_zinc_N. 1 hit.
    [Graphical view ]
    SUPFAMi SSF50129. SSF50129. 2 hits.
    PROSITEi PS00059. ADH_ZINC. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Cloning and sequencing of cDNA encoding the complete mouse liver alcohol dehydrogenase."
      Edenberg H.J., Zhang K., Fong K., Bosron W.F., Li T.-K.
      Proc. Natl. Acad. Sci. U.S.A. 82:2262-2266(1985) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    2. "Molecular analysis of mouse alcohol dehydrogenase: nucleotide sequence of the Adh-1 gene and genetic mapping of a related nucleotide sequence to chromosome 3."
      Ceci J.D., Zheng Y.W., Felder M.R.
      Gene 59:171-182(1987) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    3. "Structure of the mouse Adh-1 gene and identification of a deletion in a long alternating purine-pyrimidine sequence in the first intron of strains expressing low alcohol dehydrogenase activity."
      Zhang K., Bosron W.F., Edenberg H.J.
      Gene 57:27-36(1987) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: FVB/N.
      Tissue: Colon and Kidney.
    5. "Isolation of genomic DNA fragments corresponding to genes modulated in vivo by a transcription factor."
      Caubin J., Iglesias T., Bernal J., Munoz A., Marquez G., Barbero J.L., Zaballos A.
      Nucleic Acids Res. 22:4132-4138(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 8-51.
    6. "Androgen induction of alcohol dehydrogenase in mouse kidney. Studies with a cDNA probe confirmed by nucleotide sequence analysis."
      Ceci J.D., Lawther R., Duester G., Hatfield G.W., Smith M., O'Malley M.P., Felder M.R.
      Gene 41:217-224(1986) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 224-375.
      Strain: SWR/J.
      Tissue: Liver.
    7. "Cloning of the mouse class IV alcohol dehydrogenase (retinol dehydrogenase) cDNA and tissue-specific expression patterns of the murine ADH gene family."
      Zgombic-Knight M., Ang H.L., Foglio M.H., Duester G.
      J. Biol. Chem. 270:10868-10877(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: TISSUE SPECIFICITY.
      Strain: FVB/N.
    8. "SIRT5-mediated lysine desuccinylation impacts diverse metabolic pathways."
      Park J., Chen Y., Tishkoff D.X., Peng C., Tan M., Dai L., Xie Z., Zhang Y., Zwaans B.M., Skinner M.E., Lombard D.B., Zhao Y.
      Mol. Cell 50:919-930(2013) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUCCINYLATION [LARGE SCALE ANALYSIS] AT LYS-234 AND LYS-340, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Liver.

    Entry informationi

    Entry nameiADH1_MOUSE
    AccessioniPrimary (citable) accession number: P00329
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 21, 1986
    Last sequence update: January 23, 2007
    Last modified: October 1, 2014
    This is version 139 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3