Reviewed, UniProtKB/Swiss-Prot P00329 (ADH1_MOUSE)

Names and origin

Protein names

Recommended name:
    Alcohol dehydrogenase 1
    EC=1.1.1.1
Alternative name(s):
    Alcohol dehydrogenase A subunit
    ADH-A2

Gene names

Name:	Adh1
Synonyms:	Adh-1

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus

Protein attributes

Sequence length	375 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Catalytic activity	An alcohol + NAD+ = an aldehyde or ketone + NADH.
Cofactor	Binds 2 zinc ions per subunit.
Subunit structure	Dimer of identical or non-identical chains of three types (A, B, C), which are coded by 3 separate genes at different loci.
Subcellular location	Cytoplasm.
Tissue specificity	Expressed at high levels in the liver, small intestine and eye, at moderate levels in kidney, ovary and uterus, and at low levels in the spinal cord, thymus, heart, stomach mucosa, skin and testis. Ref.7
Sequence similarities	Belongs to the zinc-containing alcohol dehydrogenase family. Class-I subfamily.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	Metal-binding NAD Zinc
Molecular function	Oxidoreductase
Gene Ontology (GO)
Biological process	behavioral response to ethanol Inferred from mutant phenotype. Source: MGI ethanol catabolic process Inferred from direct assay. Source: MGI oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW response to retinoic acid Inferred from direct assay. Source: MGI response to testosterone stimulus Inferred from direct assay. Source: MGI retinoic acid metabolic process Inferred from mutant phenotype. Source: MGI retinol metabolic process Inferred from mutant phenotype. Source: MGI
Cellular component	mitochondrion Inferred from direct assay. Source: MGI
Molecular function	alcohol dehydrogenase (NAD) activity Inferred from direct assay. Source: MGI protein homodimerization activity Inferred from physical interaction. Source: MGI zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed
	Chain	2 – 375	374	Alcohol dehydrogenase 1		PRO_0000160666
Regions
	Nucleotide binding	200 – 205	6	NAD By similarity
	Nucleotide binding	293 – 295	3	NAD By similarity
Sites
	Metal binding	47	1	Zinc 1; catalytic
	Metal binding	68	1	Zinc 1; catalytic
	Metal binding	98	1	Zinc 2
	Metal binding	101	1	Zinc 2
	Metal binding	104	1	Zinc 2
	Metal binding	112	1	Zinc 2
	Metal binding	175	1	Zinc 1; catalytic
	Binding site	224	1	NAD By similarity
	Binding site	229	1	NAD By similarity
	Binding site	370	1	NAD By similarity

Sequences

	Sequence		Length	Mass (Da)
	P00329-1 [UniParc]. Last modified January 23, 2007. Version 2. Checksum: 7B8AF94C95D35108	FASTA	375	39,771
	10 20 30 40 50 60 MSTAGKVIKC KAAVLWELHK PFTIEDIEVA PPKAHEVRIK MVATGVCRSD DHVVSGTLVT 70 80 90 100 110 120 PLPAVLGHEG AGIVESVGEG VTCVKPGDKV IPLFSPQCGE CRICKHPESN FCSRSDLLMP 130 140 150 160 170 180 RGTLREGTSR FSCKGKQIHN FISTSTFSQY TVVDDIAVAK IDGASPLDKV CLIGCGFSTG 190 200 210 220 230 240 YGSAVKVAKV TPGSTCAVFG LGGVGLSVII GCKAAGAARI IAVDINKDKF AKAKELGATE 250 260 270 280 290 300 CINPQDYSKP IQEVLQEMTD GGVDFSFEVI GRLDTMTSAL LSCHAACGVS VVVGVPPNAQ 310 320 330 340 350 360 NLSMNPMLLL LGRTWKGAIF GGFKSKDSVP KLVADFMAKK FPLDPLITHV LPFEKINEAF 370 DLLRSGKSIR TVLTF

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning and sequencing of cDNA encoding the complete mouse liver alcohol dehydrogenase." Edenberg H.J., Zhang K., Fong K., Bosron W.F., Li T.-K. Proc. Natl. Acad. Sci. U.S.A. 82:2262-2266(1985) [PubMed: 3157987] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"Molecular analysis of mouse alcohol dehydrogenase: nucleotide sequence of the Adh-1 gene and genetic mapping of a related nucleotide sequence to chromosome 3." Ceci J.D., Zheng Y.W., Felder M.R. Gene 59:171-182(1987) [PubMed: 2893758] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"Structure of the mouse Adh-1 gene and identification of a deletion in a long alternating purine-pyrimidine sequence in the first intron of strains expressing low alcohol dehydrogenase activity." Zhang K., Bosron W.F., Edenberg H.J. Gene 57:27-36(1987) [PubMed: 3428612] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: FVB/N. Tissue: Colon and Kidney.
[5]	"Isolation of genomic DNA fragments corresponding to genes modulated in vivo by a transcription factor." Caubin J., Iglesias T., Bernal J., Munoz A., Marquez G., Barbero J.L., Zaballos A. Nucleic Acids Res. 22:4132-4138(1994) [PubMed: 7937138] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 8-51.
[6]	"Androgen induction of alcohol dehydrogenase in mouse kidney. Studies with a cDNA probe confirmed by nucleotide sequence analysis." Ceci J.D., Lawther R., Duester G., Hatfield G.W., Smith M., O'Malley M.P., Felder M.R. Gene 41:217-224(1986) [PubMed: 3011597] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 224-375. Strain: SWR/J. Tissue: Liver.
[7]	"Cloning of the mouse class IV alcohol dehydrogenase (retinol dehydrogenase) cDNA and tissue-specific expression patterns of the murine ADH gene family." Zgombic-Knight M., Ang H.L., Foglio M.H., Duester G. J. Biol. Chem. 270:10868-10877(1995) [PubMed: 7738026] [Abstract] Cited for: TISSUE SPECIFICITY. Strain: FVB/N.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	M18480 , M18472, M18473, M18474, M18475, M18476, M18477, M18478 Genomic DNA. Translation: AAA37178.1. M22679 , M22671, M22672, M22673, M22674, M22675, M22676, M22677 Genomic DNA. Translation: AAA37179.1. M11307 mRNA. Translation: AAA37180.1. BC013477 mRNA. Translation: AAH13477.1. BC054467 mRNA. Translation: AAH54467.1. Z32540 Genomic DNA. No translation available. M22611 mRNA. Translation: AAA37181.1.
IPI	IPI00221400.
PIR	DEMSAA. A27322.
RefSeq	NP_031435.1.
UniGene	Mm.2409 Mm.412004
3D structure databases
HSSP	HSSP built from PDB template 1HT0 based on UniProtKB P00326.
SMR	P00329. Positions 2-375.
ModBase	Search...
Protein-protein interaction databases
STRING	P00329.
PTM databases
PhosphoSite	P00329.
Proteomic databases
PRIDE	P00329.
Genome annotation databases
Ensembl	ENSMUST00000004232; ENSMUSP00000004232; ENSMUSG00000074207; Mus musculus. [Genome view]
GeneID	11522.
KEGG	mmu:11522.
UCSC	uc008rnf.1. mouse.
Organism-specific databases
CTD	11522.
MGI	MGI:87921. Adh1.
Phylogenomic databases
HOGENOM	P00329.
HOVERGEN	P00329.
OMA	GYKSKES.
Enzyme and pathway databases
BRENDA	1.1.1.1. 244.
Gene expression databases
ArrayExpress	P00329.
Bgee	P00329.
CleanEx	MM_ADH1.
Genevestigator	P00329.
GermOnline	ENSMUSG00000074207. Mus musculus.
Family and domain databases
InterPro	IPR013154. ADH_GroES-like. IPR002085. ADH_SF_Zn. IPR013149. ADH_Zn-bd. IPR002328. ADH_Zn_CS. [Graphical view]
PANTHER	PTHR11695. ADH_Sf_Zn. 1 hit.
Pfam	PF08240. ADH_N. 1 hit. PF00107. ADH_zinc_N. 1 hit. [Graphical view]
PROSITE	PS00059. ADH_ZINC. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	278960.
SOURCE	Search...

Entry information

Entry name

ADH1_MOUSE

Accession

Primary (citable) accession number: P00329

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 21, 1986
Last sequence update:	January 23, 2007
Last modified:	November 3, 2009
This is version 98 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)