Alcohol dehydrogenase 1 - Mus musculus (Mouse)

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P00329 (ADH1_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 3, 2013. Version 127. History...

Clusters with 100%, 90%, 50% identity |

Documents (2) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Alcohol dehydrogenase 1
EC=1.1.1.1

Alternative name(s):
ADH-A2
Alcohol dehydrogenase A subunit

Gene names

Name:	Adh1
Synonyms:	Adh-1

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	375 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Catalytic activity	An alcohol + NAD⁺ = an aldehyde or ketone + NADH.
Cofactor	Binds 2 zinc ions per subunit.
Subunit structure	Dimer of identical or non-identical chains of three types (A, B, C), which are coded by 3 separate genes at different loci.
Subcellular location	Cytoplasm.
Tissue specificity	Expressed at high levels in the liver, small intestine and eye, at moderate levels in kidney, ovary and uterus, and at low levels in the spinal cord, thymus, heart, stomach mucosa, skin and testis. Ref.7
Sequence similarities	Belongs to the zinc-containing alcohol dehydrogenase family. Class-I subfamily.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	Metal-binding NAD Zinc
Molecular function	Oxidoreductase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	behavioral response to ethanol Inferred from mutant phenotype PubMed 10358022. Source: MGI ethanol catabolic process Inferred from direct assay PubMed 518534. Source: MGI response to retinoic acid Inferred from direct assay PubMed 7748347. Source: MGI response to testosterone stimulus Inferred from direct assay PubMed 8973327. Source: MGI retinoic acid metabolic process Inferred from mutant phenotype PubMed 10358022 PubMed 12851412 PubMed 15193143. Source: MGI retinol metabolic process Inferred from mutant phenotype PubMed 10358022 PubMed 12851412 PubMed 15193143. Source: MGI
Cellular_component	mitochondrion Inferred from direct assay PubMed 3996732. Source: MGI
Molecular_function	alcohol dehydrogenase (NAD) activity Inferred from direct assay PubMed 3996732 PubMed 518534 PubMed 6370228 PubMed 6816803 PubMed 7026729. Source: MGI nucleotide binding Inferred from electronic annotation. Source: InterPro zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Initiator methionine

Removed

Chain

2 – 375

374

Alcohol dehydrogenase 1

PRO_0000160666

Regions

Nucleotide binding

200 – 205

NAD By similarity

Nucleotide binding

293 – 295

NAD By similarity

Sites

Metal binding

Zinc 1; catalytic

Metal binding

Zinc 1; catalytic

Metal binding

Zinc 2

Metal binding

101

Zinc 2

Metal binding

104

Zinc 2

Metal binding

112

Zinc 2

Metal binding

175

Zinc 1; catalytic

Binding site

224

NAD By similarity

Binding site

229

NAD By similarity

Binding site

370

NAD By similarity

Sequences

Sequence

Length

Mass (Da)

Tools

P00329 [UniParc].

Last modified January 23, 2007. Version 2.
Checksum: 7B8AF94C95D35108
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FASTA

375

39,771

        10         20         30         40         50         60 
MSTAGKVIKC KAAVLWELHK PFTIEDIEVA PPKAHEVRIK MVATGVCRSD DHVVSGTLVT 

        70         80         90        100        110        120 
PLPAVLGHEG AGIVESVGEG VTCVKPGDKV IPLFSPQCGE CRICKHPESN FCSRSDLLMP 

       130        140        150        160        170        180 
RGTLREGTSR FSCKGKQIHN FISTSTFSQY TVVDDIAVAK IDGASPLDKV CLIGCGFSTG 

       190        200        210        220        230        240 
YGSAVKVAKV TPGSTCAVFG LGGVGLSVII GCKAAGAARI IAVDINKDKF AKAKELGATE 

       250        260        270        280        290        300 
CINPQDYSKP IQEVLQEMTD GGVDFSFEVI GRLDTMTSAL LSCHAACGVS VVVGVPPNAQ 

       310        320        330        340        350        360 
NLSMNPMLLL LGRTWKGAIF GGFKSKDSVP KLVADFMAKK FPLDPLITHV LPFEKINEAF 

       370 
DLLRSGKSIR TVLTF

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning and sequencing of cDNA encoding the complete mouse liver alcohol dehydrogenase." Edenberg H.J., Zhang K., Fong K., Bosron W.F., Li T.-K. Proc. Natl. Acad. Sci. U.S.A. 82:2262-2266(1985) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"Molecular analysis of mouse alcohol dehydrogenase: nucleotide sequence of the Adh-1 gene and genetic mapping of a related nucleotide sequence to chromosome 3." Ceci J.D., Zheng Y.W., Felder M.R. Gene 59:171-182(1987) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"Structure of the mouse Adh-1 gene and identification of a deletion in a long alternating purine-pyrimidine sequence in the first intron of strains expressing low alcohol dehydrogenase activity." Zhang K., Bosron W.F., Edenberg H.J. Gene 57:27-36(1987) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: FVB/N. Tissue: Colon and Kidney.
[5]	"Isolation of genomic DNA fragments corresponding to genes modulated in vivo by a transcription factor." Caubin J., Iglesias T., Bernal J., Munoz A., Marquez G., Barbero J.L., Zaballos A. Nucleic Acids Res. 22:4132-4138(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 8-51.
[6]	"Androgen induction of alcohol dehydrogenase in mouse kidney. Studies with a cDNA probe confirmed by nucleotide sequence analysis." Ceci J.D., Lawther R., Duester G., Hatfield G.W., Smith M., O'Malley M.P., Felder M.R. Gene 41:217-224(1986) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 224-375. Strain: SWR/J. Tissue: Liver.
[7]	"Cloning of the mouse class IV alcohol dehydrogenase (retinol dehydrogenase) cDNA and tissue-specific expression patterns of the murine ADH gene family." Zgombic-Knight M., Ang H.L., Foglio M.H., Duester G. J. Biol. Chem. 270:10868-10877(1995) [PubMed] [Europe PMC] [Abstract] Cited for: TISSUE SPECIFICITY. Strain: FVB/N.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M18480 M18478 Genomic DNA. Translation: AAA37178.1. M22679 M22677 Genomic DNA. Translation: AAA37179.1. M11307 mRNA. Translation: AAA37180.1. BC013477 mRNA. Translation: AAH13477.1. BC054467 mRNA. Translation: AAH54467.1. Z32540 Genomic DNA. No translation available. M22611 mRNA. Translation: AAA37181.1.
IPI	IPI00221400.
PIR	DEMSAA. A27322.
RefSeq	NP_031435.1. NM_007409.2.
UniGene	Mm.2409. Mm.412004.
3D structure databases
ProteinModelPortal	P00329.
SMR	P00329. Positions 2-375.
ModBase	Search...
Protein-protein interaction databases
STRING	10090.ENSMUSP00000004232.
PTM databases
PhosphoSite	P00329.
Proteomic databases
PaxDb	P00329.
PRIDE	P00329.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000004232; ENSMUSP00000004232; ENSMUSG00000074207.
GeneID	11522.
KEGG	mmu:11522.
Organism-specific databases
CTD	11522.
MGI	MGI:87921. Adh1.
Phylogenomic databases
eggNOG	COG1062.
HOGENOM	HOG000294674.
HOVERGEN	HBG000195.
InParanoid	P00329.
KO	K13951.
OMA	HPESNFC.
OrthoDB	EOG4BRWM5.
Enzyme and pathway databases
SABIO-RK	P00329.
Gene expression databases
ArrayExpress	P00329.
Bgee	P00329.
CleanEx	MM_ADH1.
Genevestigator	P00329.
GermOnline	ENSMUSG00000074207. Mus musculus.
Family and domain databases
Gene3D	3.40.50.720. 1 hit.
InterPro	IPR013149. ADH_C. IPR013154. ADH_GroES-like. IPR002085. ADH_SF_Zn-type. IPR002328. ADH_Zn_CS. IPR011032. GroES-like. IPR016040. NAD(P)-bd_dom. [Graphical view]
PANTHER	PTHR11695. PTHR11695. 1 hit.
Pfam	PF08240. ADH_N. 1 hit. PF00107. ADH_zinc_N. 1 hit. [Graphical view]
SUPFAM	SSF50129. GroES_like. 2 hits.
PROSITE	PS00059. ADH_ZINC. 1 hit. [Graphical view]
ProtoNet	Search...
Other
ChEMBL	CHEMBL3366.
NextBio	278960.
SOURCE	Search...

Entry information

Entry name

ADH1_MOUSE

Accession

Primary (citable) accession number: P00329

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 21, 1986
Last sequence update:	January 23, 2007
Last modified:	April 3, 2013
This is version 127 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Relevant documents

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Sites

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

PTM databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents