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Protein

Alcohol dehydrogenase 1B

Gene

ADH1B

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Miscellaneous

There are 7 different ADH's isozymes in human: three belongs to class-I: alpha, beta, and gamma, one to class-II: pi, one to class-III: chi, one to class-IV: ADH7 and one to class-V: ADH6.

Catalytic activityi

A primary alcohol + NAD+ = an aldehyde + NADH.
A secondary alcohol + NAD+ = a ketone + NADH.

Cofactori

Zn2+Note: Binds 2 Zn2+ ions per subunit.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi47Zinc 1; catalytic1
Metal bindingi68Zinc 1; catalytic1
Metal bindingi98Zinc 21
Metal bindingi101Zinc 21
Metal bindingi104Zinc 21
Metal bindingi112Zinc 21
Metal bindingi175Zinc 1; catalytic1
Binding sitei224NAD1
Binding sitei229NAD1
Binding sitei370NAD1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi200 – 205NAD6
Nucleotide bindingi293 – 295NAD3

GO - Molecular functioni

  • alcohol dehydrogenase activity, zinc-dependent Source: UniProtKB
  • zinc ion binding Source: UniProtKB

GO - Biological processi

  • ethanol oxidation Source: UniProtKB

Keywordsi

Molecular functionOxidoreductase
LigandMetal-binding, NAD, Zinc

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER66-321
ReactomeiR-HSA-71384 Ethanol oxidation
SABIO-RKiP00325

Chemistry databases

SwissLipidsiSLP:000001880

Names & Taxonomyi

Protein namesi
Recommended name:
Alcohol dehydrogenase 1B (EC:1.1.1.1)
Alternative name(s):
Alcohol dehydrogenase subunit beta
Gene namesi
Name:ADH1B
Synonyms:ADH2
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 4

Organism-specific databases

EuPathDBiHostDB:ENSG00000196616.12
HGNCiHGNC:250 ADH1B
MIMi103720 gene+phenotype
neXtProtiNX_P00325

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Organism-specific databases

DisGeNETi125
MalaCardsiADH1B
MIMi103720 gene+phenotype
PharmGKBiPA24571

Chemistry databases

ChEMBLiCHEMBL3284
DrugBankiDB03461 2'-Monophosphoadenosine 5'-Diphosphoribose
DB02721 4-Iodopyrazole
DB03703 Cyclohexanol
DB00898 Ethanol
DB01213 Fomepizole
DB04077 Glycerol
DB02481 N-Benzylformamide
DB04105 N-Heptylformamide
DB00157 NADH

Polymorphism and mutation databases

DMDMi113394

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved1 Publication
ChainiPRO_00001606612 – 375Alcohol dehydrogenase 1BAdd BLAST374

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylserine1 Publication1
Modified residuei23PhosphoserineCombined sources1
Modified residuei35PhosphotyrosineCombined sources1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

PaxDbiP00325
PeptideAtlasiP00325
PRIDEiP00325

PTM databases

iPTMnetiP00325
PhosphoSitePlusiP00325

Expressioni

Gene expression databases

BgeeiENSG00000196616
CleanExiHS_ADH1B
ExpressionAtlasiP00325 baseline and differential
GenevisibleiP00325 HS

Organism-specific databases

HPAiHPA047814
HPA060902

Interactioni

Subunit structurei

Dimer of identical or non-identical chains of three types; alpha, beta and gamma.

Protein-protein interaction databases

BioGridi106637, 7 interactors
IntActiP00325, 8 interactors
STRINGi9606.ENSP00000306606

Chemistry databases

BindingDBiP00325

Structurei

Secondary structure

1375
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi8 – 15Combined sources8
Beta strandi23 – 29Combined sources7
Beta strandi36 – 45Combined sources10
Helixi48 – 54Combined sources7
Beta strandi56 – 58Combined sources3
Beta strandi62 – 65Combined sources4
Beta strandi69 – 77Combined sources9
Beta strandi89 – 92Combined sources4
Beta strandi99 – 101Combined sources3
Helixi102 – 105Combined sources4
Beta strandi116 – 119Combined sources4
Beta strandi131 – 133Combined sources3
Beta strandi136 – 139Combined sources4
Turni142 – 144Combined sources3
Beta strandi147 – 154Combined sources8
Helixi155 – 157Combined sources3
Beta strandi158 – 160Combined sources3
Helixi167 – 170Combined sources4
Helixi171 – 174Combined sources4
Helixi176 – 185Combined sources10
Turni186 – 188Combined sources3
Beta strandi195 – 199Combined sources5
Helixi203 – 214Combined sources12
Beta strandi218 – 223Combined sources6
Helixi227 – 229Combined sources3
Helixi230 – 235Combined sources6
Beta strandi239 – 242Combined sources4
Helixi244 – 246Combined sources3
Helixi251 – 258Combined sources8
Turni259 – 261Combined sources3
Beta strandi262 – 268Combined sources7
Helixi273 – 282Combined sources10
Turni285 – 287Combined sources3
Beta strandi289 – 292Combined sources4
Beta strandi302 – 304Combined sources3
Helixi307 – 310Combined sources4
Beta strandi314 – 317Combined sources4
Helixi320 – 322Combined sources3
Helixi325 – 337Combined sources13
Helixi344 – 346Combined sources3
Beta strandi347 – 352Combined sources6
Helixi353 – 355Combined sources3
Helixi356 – 364Combined sources9
Beta strandi369 – 374Combined sources6

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1DEHX-ray2.20A/B2-375[»]
1HDXX-ray2.50A/B2-375[»]
1HDYX-ray2.50A/B2-375[»]
1HDZX-ray2.50A/B2-375[»]
1HSZX-ray2.20A/B2-375[»]
1HTBX-ray2.40A/B2-375[»]
1U3UX-ray1.60A/B2-375[»]
1U3VX-ray1.65A/B2-375[»]
3HUDX-ray3.20A/B2-375[»]
ProteinModelPortaliP00325
SMRiP00325
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP00325

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG0022 Eukaryota
COG1062 LUCA
HOGENOMiHOG000294674
HOVERGENiHBG000195
InParanoidiP00325
KOiK13951
OrthoDBiEOG091G08N3
PhylomeDBiP00325
TreeFamiTF300429

Family and domain databases

InterProiView protein in InterPro
IPR013149 ADH_C
IPR013154 ADH_N
IPR002328 ADH_Zn_CS
IPR011032 GroES-like_sf
IPR036291 NAD(P)-bd_dom_sf
IPR020843 PKS_ER
PfamiView protein in Pfam
PF08240 ADH_N, 1 hit
PF00107 ADH_zinc_N, 1 hit
SMARTiView protein in SMART
SM00829 PKS_ER, 1 hit
SUPFAMiSSF50129 SSF50129, 2 hits
SSF51735 SSF51735, 1 hit
PROSITEiView protein in PROSITE
PS00059 ADH_ZINC, 1 hit

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: P00325-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSTAGKVIKC KAAVLWEVKK PFSIEDVEVA PPKAYEVRIK MVAVGICRTD
60 70 80 90 100
DHVVSGNLVT PLPVILGHEA AGIVESVGEG VTTVKPGDKV IPLFTPQCGK
110 120 130 140 150
CRVCKNPESN YCLKNDLGNP RGTLQDGTRR FTCRGKPIHH FLGTSTFSQY
160 170 180 190 200
TVVDENAVAK IDAASPLEKV CLIGCGFSTG YGSAVNVAKV TPGSTCAVFG
210 220 230 240 250
LGGVGLSAVM GCKAAGAARI IAVDINKDKF AKAKELGATE CINPQDYKKP
260 270 280 290 300
IQEVLKEMTD GGVDFSFEVI GRLDTMMASL LCCHEACGTS VIVGVPPASQ
310 320 330 340 350
NLSINPMLLL TGRTWKGAVY GGFKSKEGIP KLVADFMAKK FSLDALITHV
360 370
LPFEKINEGF DLLHSGKSIR TVLTF
Length:375
Mass (Da):39,855
Last modified:January 23, 2007 - v2
Checksum:i6962B9A0F967673B
GO
Isoform 2 (identifier: P00325-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-40: Missing.

Show »
Length:335
Mass (Da):35,412
Checksum:iA92B71F22E3CAFA4
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti8I → M in AAA51592 (PubMed:3397059).Curated1
Sequence conflicti130Missing AA sequence (PubMed:6391920).Curated1
Sequence conflicti166P → K in AAA51592 (PubMed:3397059).Curated1
Sequence conflicti190V → VV in CAA33487 (PubMed:2547609).Curated1
Sequence conflicti220I → V in BAG62635 (PubMed:14702039).Curated1
Sequence conflicti230F → K in AAA51884 (PubMed:2986130).Curated1
Sequence conflicti235E → V in AAA51592 (PubMed:3397059).Curated1
Sequence conflicti338 – 344Missing in BAG62635 (PubMed:14702039).Curated7

Polymorphismi

Three alleles are known: ADH1B*1 (ADH2*1) corresponding to variant beta-1, ADH1B*2 (ADH2*2) corresponding to variant beta-2, ADH1B*3 (ADH2*3) corresponding to variant beta-3. The sequence shown is that of allele ADH1B*1. The ADH1B*2 allele frequency in orientals is approximately 75%, whereas it is less than 5% in most Caucasian populations. ADH1B variations have been associated with protection against alcohol dependence and alcohol-related aerodigestive tract cancer [MIMi:103720].5 Publications

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_00042648R → H in beta-2; allele ADH1B*2; common in Asian populations; associated with a lower risk of alcoholism. Combined sources7 PublicationsCorresponds to variant dbSNP:rs1229984Ensembl.1
Natural variantiVAR_01932257N → K2 PublicationsCorresponds to variant dbSNP:rs1041969Ensembl.1
Natural variantiVAR_01932360T → S1 PublicationCorresponds to variant dbSNP:rs6413413Ensembl.1
Natural variantiVAR_000427370R → C in beta-3/Indianapolis; allele ADH1B*3; decreased NAD(H) binding. 3 PublicationsCorresponds to variant dbSNP:rs2066702EnsemblClinVar.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0548471 – 40Missing in isoform 2. 1 PublicationAdd BLAST40

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M24317 mRNA Translation: AAA51884.1
X03350 mRNA Translation: CAA27056.1
M24316
, M24308, M24309, M24310, M24311, M24312, M24313, M24314 Genomic DNA Translation: AAB59496.1
D00137 mRNA Translation: BAA00084.1
L38290
, L38283, L38284, L38285, L38286, L38287, L38288, L38289 Genomic DNA Translation: AAB48003.1
X15447
, X15448, X15449, X15450, X15451, X15452, X15453, X15454, X15455 Genomic DNA Translation: CAA33487.1
AF153821 mRNA Translation: AAD37446.1
DQ017646 Genomic DNA Translation: AAY22180.1
AK301018 mRNA Translation: BAG62635.1
AC097530 Genomic DNA No translation available.
BC033009 mRNA Translation: AAH33009.1
M21692 mRNA Translation: AAA51592.1
AF040967 Genomic DNA Translation: AAB96912.1
CCDSiCCDS34033.1 [P00325-1]
CCDS68761.1 [P00325-2]
PIRiA23607 DEHUAB
RefSeqiNP_000659.2, NM_000668.5
NP_001273579.1, NM_001286650.1
UniGeneiHs.4

Genome annotation databases

EnsembliENST00000305046; ENSP00000306606; ENSG00000196616
ENST00000394887; ENSP00000378351; ENSG00000196616
GeneIDi125
KEGGihsa:125
UCSCiuc011cei.3 human [P00325-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Similar proteinsi

Entry informationi

Entry nameiADH1B_HUMAN
AccessioniPrimary (citable) accession number: P00325
Secondary accession number(s): A8MYN5
, B4DRS9, B4DVC3, Q13711, Q4ZGI9, Q96KI7
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: January 23, 2007
Last modified: March 28, 2018
This is version 195 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

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