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Protein

Flavodoxin-2

Gene

nifF

Organism
Azotobacter vinelandii
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Low-potential electron donor to a number of redox enzymes. NifF is the electron donor to nitrogenase.

Cofactori

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Electron transport, Nitrogen fixation, Transport

Keywords - Ligandi

Flavoprotein, FMN

Names & Taxonomyi

Protein namesi
Recommended name:
Flavodoxin-2
Gene namesi
Name:nifF
OrganismiAzotobacter vinelandii
Taxonomic identifieri354 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaeAzotobacter

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemoved2 Publications
Chaini2 – 180179Flavodoxin-2PRO_0000171605Add
BLAST

Interactioni

Subunit structurei

Monomer.

Protein-protein interaction databases

STRINGi322710.Avin_01710.

Structurei

Secondary structure

1
180
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi4 – 85Combined sources
Beta strandi11 – 133Combined sources
Helixi14 – 2310Combined sources
Turni28 – 303Combined sources
Helixi37 – 393Combined sources
Helixi42 – 465Combined sources
Beta strandi49 – 568Combined sources
Turni59 – 613Combined sources
Helixi66 – 683Combined sources
Helixi75 – 828Combined sources
Beta strandi91 – 977Combined sources
Turni100 – 1023Combined sources
Turni104 – 1085Combined sources
Helixi109 – 11911Combined sources
Turni120 – 1223Combined sources
Beta strandi124 – 1263Combined sources
Beta strandi142 – 15211Combined sources
Turni154 – 1563Combined sources
Helixi158 – 1603Combined sources
Helixi161 – 17212Combined sources
Helixi173 – 1764Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1YOBX-ray2.25A/B2-180[»]
ProteinModelPortaliP00324.
SMRiP00324. Positions 2-180.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP00324.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini4 – 173170Flavodoxin-likePROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the flavodoxin family.Curated
Contains 1 flavodoxin-like domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiENOG4108TGK. Bacteria.
COG0716. LUCA.

Family and domain databases

Gene3Di3.40.50.360. 1 hit.
InterProiIPR001094. Flavdoxin-like.
IPR008254. Flavodoxin/NO_synth.
IPR001226. Flavodoxin_CS.
IPR010086. Flavodoxin_lc.
IPR029039. Flavoprotein-like_dom.
[Graphical view]
PfamiPF00258. Flavodoxin_1. 1 hit.
[Graphical view]
PIRSFiPIRSF038996. FldA. 1 hit.
PRINTSiPR00369. FLAVODOXIN.
SUPFAMiSSF52218. SSF52218. 1 hit.
TIGRFAMsiTIGR01752. flav_long. 1 hit.
PROSITEiPS00201. FLAVODOXIN. 1 hit.
PS50902. FLAVODOXIN_LIKE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P00324-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAKIGLFFGS NTGKTRKVAK SIKKRFDDET MSDALNVNRV SAEDFAQYQF
60 70 80 90 100
LILGTPTLGE GELPGLSSDC ENESWEEFLP KIEGLDFSGK TVALFGLGDQ
110 120 130 140 150
VGYPENYLDA LGELYSFFKD RGAKIVGSWS TDGYEFESSE AVVDGKFVGL
160 170 180
ALDLDNQSGK TDERVAAWLA QIAPEFGLSL
Length:180
Mass (Da):19,663
Last modified:January 23, 2007 - v2
Checksum:i8B1B43F23AB5E8B4
GO

Mass spectrometryi

Molecular mass is 19533±5 Da from positions 2 - 180. Determined by ESI. 1 Publication

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M20568 Genomic DNA. Translation: AAA64735.1.
J03519 Genomic DNA. Translation: AAA22154.1.
PIRiA29935. FXAVEP.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M20568 Genomic DNA. Translation: AAA64735.1.
J03519 Genomic DNA. Translation: AAA22154.1.
PIRiA29935. FXAVEP.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1YOBX-ray2.25A/B2-180[»]
ProteinModelPortaliP00324.
SMRiP00324. Positions 2-180.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi322710.Avin_01710.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Phylogenomic databases

eggNOGiENOG4108TGK. Bacteria.
COG0716. LUCA.

Miscellaneous databases

EvolutionaryTraceiP00324.

Family and domain databases

Gene3Di3.40.50.360. 1 hit.
InterProiIPR001094. Flavdoxin-like.
IPR008254. Flavodoxin/NO_synth.
IPR001226. Flavodoxin_CS.
IPR010086. Flavodoxin_lc.
IPR029039. Flavoprotein-like_dom.
[Graphical view]
PfamiPF00258. Flavodoxin_1. 1 hit.
[Graphical view]
PIRSFiPIRSF038996. FldA. 1 hit.
PRINTSiPR00369. FLAVODOXIN.
SUPFAMiSSF52218. SSF52218. 1 hit.
TIGRFAMsiTIGR01752. flav_long. 1 hit.
PROSITEiPS00201. FLAVODOXIN. 1 hit.
PS50902. FLAVODOXIN_LIKE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Physical and genetic map of the major nif gene cluster from Azotobacter vinelandii."
    Jacobson M.R., Brigle K.E., Bennett L.T., Setterquist R.A., Wilson M.S., Cash V.L., Beynon J., Newton W.E., Dean D.R.
    J. Bacteriol. 171:1017-1027(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "Isolation, sequencing, and mutagenesis of the nifF gene encoding flavodoxin from Azotobacter vinelandii."
    Bennett L., Jacobson M., Dean D.R.
    J. Biol. Chem. 263:1364-1369(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  3. "Complete amino acid sequence of azotoflavin, a flavodoxin from Azotobacter vinelandii."
    Tanaka M., Haniu M., Yasunobu K.T., Yoch D.C.
    Biochemistry 16:3525-3537(1977) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 2-180.
    Strain: ATCC 13705 / OP1 / DSM 366 / NCIB 11614 / LMG 3878 / UW.
  4. "Flavodoxin 1 of Azotobacter vinelandii: characterization and role in electron donation to purified assimilatory nitrate reductase."
    Gangeswaran R., Eady R.R.
    Biochem. J. 317:103-108(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 2-21, MASS SPECTROMETRY.
    Strain: OP / UW136.
  5. "Apparent local stability of the secondary structure of Azotobacter vinelandii holoflavodoxin II as probed by hydrogen exchange: implications for redox potential regulation and flavodoxin folding."
    Steensma E., Nijman M.J.M., Bollen Y.J.M., de Jager P.A., van den Berg W.A.M., van Dongen W.M.A.M., van Mierlo C.P.M.
    Protein Sci. 7:306-317(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: STRUCTURE BY NMR.
    Strain: ATCC 478 / NRS 16 / DSM 2289 / VKM B-1617.

Entry informationi

Entry nameiFLAV_AZOVI
AccessioniPrimary (citable) accession number: P00324
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: January 23, 2007
Last modified: July 6, 2016
This is version 95 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Direct protein sequencing

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.