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Protein

Flavodoxin-2

Gene

nifF

Organism
Azotobacter vinelandii
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Low-potential electron donor to a number of redox enzymes. NifF is the electron donor to nitrogenase.

Cofactori

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Electron transport, Nitrogen fixation, Transport

Keywords - Ligandi

Flavoprotein, FMN

Names & Taxonomyi

Protein namesi
Recommended name:
Flavodoxin-2
Gene namesi
Name:nifF
OrganismiAzotobacter vinelandii
Taxonomic identifieri354 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaeAzotobacter

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved2 Publications
ChainiPRO_00001716052 – 180Flavodoxin-2Add BLAST179

Interactioni

Subunit structurei

Monomer.

Protein-protein interaction databases

STRINGi322710.Avin_01710.

Structurei

Secondary structure

1180
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi4 – 8Combined sources5
Beta strandi11 – 13Combined sources3
Helixi14 – 23Combined sources10
Turni28 – 30Combined sources3
Helixi37 – 39Combined sources3
Helixi42 – 46Combined sources5
Beta strandi49 – 56Combined sources8
Turni59 – 61Combined sources3
Helixi66 – 68Combined sources3
Helixi75 – 82Combined sources8
Beta strandi91 – 97Combined sources7
Turni100 – 102Combined sources3
Turni104 – 108Combined sources5
Helixi109 – 119Combined sources11
Turni120 – 122Combined sources3
Beta strandi124 – 126Combined sources3
Beta strandi142 – 152Combined sources11
Turni154 – 156Combined sources3
Helixi158 – 160Combined sources3
Helixi161 – 172Combined sources12
Helixi173 – 176Combined sources4

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1YOBX-ray2.25A/B2-180[»]
ProteinModelPortaliP00324.
SMRiP00324.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP00324.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini4 – 173Flavodoxin-likePROSITE-ProRule annotationAdd BLAST170

Sequence similaritiesi

Belongs to the flavodoxin family.Curated
Contains 1 flavodoxin-like domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiENOG4108TGK. Bacteria.
COG0716. LUCA.

Family and domain databases

Gene3Di3.40.50.360. 1 hit.
InterProiIPR001094. Flavdoxin-like.
IPR008254. Flavodoxin/NO_synth.
IPR001226. Flavodoxin_CS.
IPR010086. Flavodoxin_lc.
IPR029039. Flavoprotein-like_dom.
[Graphical view]
PfamiPF00258. Flavodoxin_1. 1 hit.
[Graphical view]
PIRSFiPIRSF038996. FldA. 1 hit.
PRINTSiPR00369. FLAVODOXIN.
SUPFAMiSSF52218. SSF52218. 1 hit.
TIGRFAMsiTIGR01752. flav_long. 1 hit.
PROSITEiPS00201. FLAVODOXIN. 1 hit.
PS50902. FLAVODOXIN_LIKE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P00324-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAKIGLFFGS NTGKTRKVAK SIKKRFDDET MSDALNVNRV SAEDFAQYQF
60 70 80 90 100
LILGTPTLGE GELPGLSSDC ENESWEEFLP KIEGLDFSGK TVALFGLGDQ
110 120 130 140 150
VGYPENYLDA LGELYSFFKD RGAKIVGSWS TDGYEFESSE AVVDGKFVGL
160 170 180
ALDLDNQSGK TDERVAAWLA QIAPEFGLSL
Length:180
Mass (Da):19,663
Last modified:January 23, 2007 - v2
Checksum:i8B1B43F23AB5E8B4
GO

Mass spectrometryi

Molecular mass is 19533±5 Da from positions 2 - 180. Determined by ESI. 1 Publication

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M20568 Genomic DNA. Translation: AAA64735.1.
J03519 Genomic DNA. Translation: AAA22154.1.
PIRiA29935. FXAVEP.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M20568 Genomic DNA. Translation: AAA64735.1.
J03519 Genomic DNA. Translation: AAA22154.1.
PIRiA29935. FXAVEP.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1YOBX-ray2.25A/B2-180[»]
ProteinModelPortaliP00324.
SMRiP00324.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi322710.Avin_01710.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Phylogenomic databases

eggNOGiENOG4108TGK. Bacteria.
COG0716. LUCA.

Miscellaneous databases

EvolutionaryTraceiP00324.

Family and domain databases

Gene3Di3.40.50.360. 1 hit.
InterProiIPR001094. Flavdoxin-like.
IPR008254. Flavodoxin/NO_synth.
IPR001226. Flavodoxin_CS.
IPR010086. Flavodoxin_lc.
IPR029039. Flavoprotein-like_dom.
[Graphical view]
PfamiPF00258. Flavodoxin_1. 1 hit.
[Graphical view]
PIRSFiPIRSF038996. FldA. 1 hit.
PRINTSiPR00369. FLAVODOXIN.
SUPFAMiSSF52218. SSF52218. 1 hit.
TIGRFAMsiTIGR01752. flav_long. 1 hit.
PROSITEiPS00201. FLAVODOXIN. 1 hit.
PS50902. FLAVODOXIN_LIKE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiFLAV_AZOVI
AccessioniPrimary (citable) accession number: P00324
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: January 23, 2007
Last modified: November 2, 2016
This is version 98 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Direct protein sequencing

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.