P00324 (FLAV_AZOVI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Flavodoxin-2

Gene names

Name:

nifF

Organism

Azotobacter vinelandii

Taxonomic identifier

354 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Azotobacter group › Azotobacter

Protein attributes

Sequence length	180 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Low-potential electron donor to a number of redox enzymes. NifF is the electron donor to nitrogenase.
Cofactor	FMN.
Subunit structure	Monomer.
Sequence similarities	Belongs to the flavodoxin family. Contains 1 flavodoxin-like domain.
Mass spectrometry	Molecular mass is 19533±5 Da from positions 2 - 180. Determined by ESI. Ref.4

Ontologies

Keywords
Biological process	Electron transport Nitrogen fixation Transport
Ligand	FMN Flavoprotein
Technical term	3D-structure Direct protein sequencing
Gene Ontology (GO)
Biological_process	electron transport chain Inferred from electronic annotation. Source: UniProtKB-KW nitrogen fixation Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	FMN binding Inferred from electronic annotation. Source: InterPro electron carrier activity Inferred from electronic annotation. Source: InterPro iron ion binding Inferred from electronic annotation. Source: InterPro oxidoreductase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Initiator methionine

1

1

Removed Ref.3 Ref.4

Chain

2 – 180

179

Flavodoxin-2

PRO_0000171605

Regions

Domain

4 – 173

170

Flavodoxin-like

Secondary structure

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180

Helix Strand Turn

Details...

Beta strand

4 – 8

5

Beta strand

11 – 13

3

Helix

14 – 23

10

Turn

28 – 30

3

Helix

37 – 39

3

Helix

42 – 46

5

Beta strand

49 – 56

8

Turn

59 – 61

3

Helix

66 – 68

3

Helix

75 – 82

8

Beta strand

91 – 97

7

Turn

100 – 102

3

Turn

104 – 108

5

Helix

109 – 119

11

Turn

120 – 122

3

Beta strand

124 – 126

3

Beta strand

142 – 152

11

Turn

154 – 156

3

Helix

158 – 160

3

Helix

161 – 172

12

Helix

173 – 176

4

Sequences

	Sequence		Length	Mass (Da)
	P00324 [UniParc]. Last modified January 23, 2007. Version 2. Checksum: 8B1B43F23AB5E8B4	FASTA	180	19,663
	10 20 30 40 50 60 MAKIGLFFGS NTGKTRKVAK SIKKRFDDET MSDALNVNRV SAEDFAQYQF LILGTPTLGE 70 80 90 100 110 120 GELPGLSSDC ENESWEEFLP KIEGLDFSGK TVALFGLGDQ VGYPENYLDA LGELYSFFKD 130 140 150 160 170 180 RGAKIVGSWS TDGYEFESSE AVVDGKFVGL ALDLDNQSGK TDERVAAWLA QIAPEFGLSL

References

[1]	"Physical and genetic map of the major nif gene cluster from Azotobacter vinelandii." Jacobson M.R., Brigle K.E., Bennett L.T., Setterquist R.A., Wilson M.S., Cash V.L., Beynon J., Newton W.E., Dean D.R. J. Bacteriol. 171:1017-1027(1989) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Isolation, sequencing, and mutagenesis of the nifF gene encoding flavodoxin from Azotobacter vinelandii." Bennett L., Jacobson M., Dean D.R. J. Biol. Chem. 263:1364-1369(1988) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"Complete amino acid sequence of azotoflavin, a flavodoxin from Azotobacter vinelandii." Tanaka M., Haniu M., Yasunobu K.T., Yoch D.C. Biochemistry 16:3525-3537(1977) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 2-180. Strain: ATCC 13705 / OP1 / DSM 366 / NCIB 11614 / LMG 3878 / UW.
[4]	"Flavodoxin 1 of Azotobacter vinelandii: characterization and role in electron donation to purified assimilatory nitrate reductase." Gangeswaran R., Eady R.R. Biochem. J. 317:103-108(1996) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 2-21, MASS SPECTROMETRY. Strain: OP / UW136.
[5]	"Apparent local stability of the secondary structure of Azotobacter vinelandii holoflavodoxin II as probed by hydrogen exchange: implications for redox potential regulation and flavodoxin folding." Steensma E., Nijman M.J.M., Bollen Y.J.M., de Jager P.A., van den Berg W.A.M., van Dongen W.M.A.M., van Mierlo C.P.M. Protein Sci. 7:306-317(1998) [PubMed] [Europe PMC] [Abstract] Cited for: STRUCTURE BY NMR. Strain: ATCC 478 / NRS 16 / DSM 2289 / VKM B-1617.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

M20568 Genomic DNA. Translation: AAA64735.1.
J03519 Genomic DNA. Translation: AAA22154.1.

PIR

FXAVEP. A29935.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1YOB	X-ray	2.25	A/B	2-179	[»]

ProteinModelPortal

P00324.

SMR

P00324. Positions 2-180.

ModBase

Search...

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Family and domain databases

InterPro

IPR001094. Flavdoxin.
IPR008254. Flavodoxin/NO_synth.
IPR001226. Flavodoxin_CS.
IPR010086. Flavodoxin_lc.
[Graphical view]

Pfam

PF00258. Flavodoxin_1. 1 hit.
[Graphical view]

PIRSF

PIRSF038996. FldA. 1 hit.

PRINTS

PR00369. FLAVODOXIN.

TIGRFAMs

TIGR01752. flav_long. 1 hit.

PROSITE

PS00201. FLAVODOXIN. 1 hit.
PS50902. FLAVODOXIN_LIKE. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

EvolutionaryTrace

P00324.

Entry information

Entry name

FLAV_AZOVI

Accession

Primary (citable) accession number: P00324

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 21, 1986
Last sequence update:	January 23, 2007
Last modified:	April 3, 2013
This is version 84 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program