P00323 (FLAV_DESVH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Flavodoxin

Gene names

Ordered Locus Names:

DVU_2680

Organism

Desulfovibrio vulgaris (strain Hildenborough / ATCC 29579 / NCIMB 8303) [Reference proteome] [HAMAP]

Taxonomic identifier

882 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › delta/epsilon subdivisions › Deltaproteobacteria › Desulfovibrionales › Desulfovibrionaceae › Desulfovibrio › Desulfovibrio vulgaris

Protein attributes

Sequence length	148 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Low-potential electron donor to a number of redox enzymes.
Cofactor	FMN.
Sequence similarities	Belongs to the flavodoxin family. Contains 1 flavodoxin-like domain.

Ontologies

Keywords
Biological process	Electron transport Transport
Ligand	FMN Flavoprotein
Technical term	3D-structure Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	electron transport chain Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	FMN binding Inferred from electronic annotation. Source: InterPro electron carrier activity Inferred from electronic annotation. Source: InterPro iron ion binding Inferred from electronic annotation. Source: InterPro oxidoreductase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 148

148

Flavodoxin

PRO_0000171620

Regions

Domain

4 – 145

142

Flavodoxin-like

Experimental info

Sequence conflict

28

1

D → N Ref.2

Secondary structure

1

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148

Helix Strand Turn

Details...

Beta strand

3 – 9

7

Beta strand

11 – 13

3

Helix

14 – 29

16

Beta strand

32 – 37

6

Helix

38 – 40

3

Turn

44 – 49

6

Beta strand

51 – 57

7

Beta strand

62 – 64

3

Turn

69 – 71

3

Helix

72 – 76

5

Helix

78 – 80

3

Beta strand

87 – 94

8

Beta strand

98 – 100

3

Helix

103 – 114

12

Beta strand

124 – 128

5

Helix

130 – 133

4

Helix

134 – 145

12

Sequences

	Sequence		Length	Mass (Da)
	P00323 [UniParc]. Last modified November 1, 1990. Version 2. Checksum: E07630E7047ABD3F	FASTA	148	15,823
	10 20 30 40 50 60 MPKALIVYGS TTGNTEYTAE TIARELADAG YEVDSRDAAS VEAGGLFEGF DLVLLGCSTW 70 80 90 100 110 120 GDDSIELQDD FIPLFDSLEE TGAQGRKVAC FGCGDSSYEY FCGAVDAIEE KLKNLGAEIV 130 140 QDGLRIDGDP RAARDDIVGW AHDVRGAI

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning, nucleotide sequence, and expression of the flavodoxin gene from Desulfovibrio vulgaris (Hildenborough)." Krey G.D., Vanin E.F., Swenson R.P. J. Biol. Chem. 263:15436-15443(1988) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Cloning and sequencing of the gene encoding flavodoxin from Desulfovibrio vulgaris Hildenborough." Curley G.P., Voordouw G. FEMS Microbiol. Lett. 49:295-299(1988) Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"Amino acid sequence of Desulfovibrio vulgaris flavodoxin." Dubourdieu M., Fox J.L. J. Biol. Chem. 252:1453-1463(1977) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE.
[4]	"The genome sequence of the anaerobic, sulfate-reducing bacterium Desulfovibrio vulgaris Hildenborough." Heidelberg J.F., Seshadri R., Haveman S.A., Hemme C.L., Paulsen I.T., Kolonay J.F., Eisen J.A., Ward N.L., Methe B.A., Brinkac L.M., Daugherty S.C., DeBoy R.T., Dodson R.J., Durkin A.S., Madupu R., Nelson W.C., Sullivan S.A., Fouts D.E. , Haft D.H., Selengut J., Peterson J.D., Davidsen T.M., Zafar N., Zhou L., Radune D., Dimitrov G., Hance M., Tran K., Khouri H.M., Gill J., Utterback T.R., Feldblyum T.V., Wall J.D., Voordouw G., Fraser C.M. Nat. Biotechnol. 22:554-559(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Hildenborough / ATCC 29579 / NCIMB 8303.
[5]	"Comparison of the crystal structures of a flavodoxin in its three oxidation states at cryogenic temperatures." Warr W., Tulinsky A., Swenson R.P., Watenpaugh K.D. J. Mol. Biol. 218:195-208(1991) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.0 ANGSTROMS).
[6]	"The binding of riboflavin-5'-phosphate in a flavoprotein: flavodoxin at 2.0-A resolution." Watenpaugh K.D., Sieker L.C., Jensen L.H. Proc. Natl. Acad. Sci. U.S.A. 70:3857-3860(1973) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.0 ANGSTROMS).
[7]	"Structure of the oxidized form of a flavodoxin at 2.5-A resolution: resolution of the phase ambiguity by anomalous scattering." Watenpaugh K.D., Sieker L.C., Jensen L.H., Legall J., Dubourdieu M. Proc. Natl. Acad. Sci. U.S.A. 69:3185-3188(1972) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.5 ANGSTROMS).
[8]	"X-ray crystal structure of the Desulfovibrio vulgaris (Hildenborough) apoflavodoxin-riboflavin complex." Walsh M.A., McCarthy A., O'Farrell P.A., McArdle P., Cunningham P.D., Mayhew S.G., Higgins T.M. Eur. J. Biochem. 258:362-371(1998) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.8 ANGSTROMS).
[9]	"Homonuclear and heteronuclear NMR studies of oxidized Desulfovibrio vulgaris flavodoxin. Sequential assignments and identification of secondary structure elements." Knauf M.A., Loehr F., Curley G.P., O'Farrell P., Mayhew S.G., Mueller F., Rueterjans H. Eur. J. Biochem. 213:167-184(1993) [PubMed] [Europe PMC] [Abstract] Cited for: STRUCTURE BY NMR.
[10]	"NMR investigation of the solution conformation of oxidized flavodoxin from Desulfovibrio vulgaris. Determination of the tertiary structure and detection of protein-bound water molecules." Knauf M.A., Loehr F., Bluemel M., Mayhew S.G., Rueterjans H. Eur. J. Biochem. 238:423-434(1996) [PubMed] [Europe PMC] [Abstract] Cited for: STRUCTURE BY NMR.
[11]	"1H and 15N resonance assignments and solution secondary structure of oxidized Desulfovibrio vulgaris flavodoxin determined by heteronuclear three-dimensional NMR spectroscopy." Stockman B.J., Euvrard A., Kloosterman D.A., Scahill T.A., Swenson R.P. J. Biomol. NMR 3:133-149(1993) [PubMed] [Europe PMC] [Abstract] Cited for: STRUCTURE BY NMR.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

J04033 Genomic DNA. Translation: AAA23367.1.
AE017285 Genomic DNA. Translation: AAS97152.1.

PIR

FXDV. A31991.

RefSeq

YP_011892.1. NC_002937.3.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1AKQ	X-ray	1.90	A	2-148	[»]
1AKR	X-ray	1.58	A	2-148	[»]
1AKT	X-ray	1.80	A	2-148	[»]
1AKU	X-ray	1.90	A	2-148	[»]
1AKV	X-ray	2.00	A	2-148	[»]
1AKW	X-ray	1.75	A	2-148	[»]
1AZL	X-ray	1.80	A	2-148	[»]
1BU5	X-ray	1.83	A/B	2-148	[»]
1C7E	X-ray	2.25	A/B	2-148	[»]
1C7F	X-ray	2.00	A/B	2-148	[»]
1F4P	X-ray	1.30	A	2-148	[»]
1FX1	X-ray	2.00	A	1-148	[»]
1I1O	X-ray	2.00	A	3-148	[»]
1J8Q	X-ray	1.35	A	3-148	[»]
1J9E	X-ray	1.44	A	3-148	[»]
1J9G	X-ray	2.40	A	3-148	[»]
1WSB	X-ray	1.80	A	3-148	[»]
1WSW	X-ray	1.69	A	3-148	[»]
1XT6	X-ray	1.80	A	3-148	[»]
1XYV	X-ray	1.79	A	3-148	[»]
1XYY	X-ray	1.70	A	3-148	[»]
2FX2	X-ray	1.90	A	3-148	[»]
3FX2	X-ray	1.90	A	3-148	[»]
4FX2	X-ray	1.90	A	3-148	[»]
5FX2	X-ray	1.90	A	3-148	[»]

ProteinModelPortal

P00323.

SMR

P00323. Positions 2-148.

ModBase

Search...

Protein-protein interaction databases

STRING

882.DVU2680.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblBacteria

AAS97152; AAS97152; DVU_2680.

GeneID

2795051.

KEGG

dvu:DVU2680.

PATRIC

32064950. VBIDesVul119526_2425.

Phylogenomic databases

eggNOG

COG0716.

OMA

HFCGAVD.

ProtClustDB

CLSK634549.

Enzyme and pathway databases

BioCyc

DVUL882:GJIL-2748-MONOMER.

Family and domain databases

InterPro

IPR010087. Flav_short.
IPR001094. Flavdoxin.
IPR008254. Flavodoxin/NO_synth.
IPR001226. Flavodoxin_CS.
[Graphical view]

Pfam

PF00258. Flavodoxin_1. 1 hit.
[Graphical view]

PRINTS

PR00369. FLAVODOXIN.

TIGRFAMs

TIGR01753. flav_short. 1 hit.

PROSITE

PS00201. FLAVODOXIN. 1 hit.
PS50902. FLAVODOXIN_LIKE. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

DrugBank

DB00140. Riboflavin.

EvolutionaryTrace

P00323.

Entry information

Entry name

FLAV_DESVH

Accession

Primary (citable) accession number: P00323

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 21, 1986
Last sequence update:	November 1, 1990
Last modified:	May 1, 2013
This is version 106 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program