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Protein

Insecticyanin-A

Gene

INSA

Organism
Manduca sexta (Tobacco hawkmoth) (Tobacco hornworm)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

This protein binds a chromophore: biliverdin IX, isomer gamma. Mixed with lipoprotein-bound carotenes, this blue protein provides hornworms with their green cryptic coloration which serves a camouflage.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Ligandi

Bile pigment, Chromophore, Pigment

Names & Taxonomyi

Protein namesi
Recommended name:
Insecticyanin-A
Short name:
INS-a
Alternative name(s):
Blue biliprotein
Gene namesi
Name:INSA
OrganismiManduca sexta (Tobacco hawkmoth) (Tobacco hornworm)
Taxonomic identifieri7130 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaLepidopteraGlossataDitrysiaBombycoideaSphingidaeSphinginaeSphinginiManduca

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002010121 – 189Insecticyanin-AAdd BLAST189

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi9 ↔ 1191 Publication
Disulfide bondi43 ↔ 1751 Publication

Keywords - PTMi

Disulfide bond

Expressioni

Tissue specificityi

Synthesized only in the caterpillars, apparently by the epidermis and secreted into the hemolymph. The protein is passed over from the larval hemolymph to that of pupae and adults and is sequestered in the eggs.

Interactioni

Subunit structurei

Homotetramer.

Structurei

Secondary structure

1189
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi3 – 8Combined sources6
Helixi20 – 23Combined sources4
Beta strandi25 – 32Combined sources8
Helixi36 – 38Combined sources3
Beta strandi42 – 50Combined sources9
Beta strandi55 – 63Combined sources9
Beta strandi66 – 76Combined sources11
Helixi80 – 83Combined sources4
Beta strandi86 – 94Combined sources9
Beta strandi97 – 101Combined sources5
Beta strandi104 – 108Combined sources5
Beta strandi110 – 121Combined sources12
Turni123 – 125Combined sources3
Beta strandi126 – 140Combined sources15
Helixi144 – 154Combined sources11
Turni155 – 159Combined sources5
Helixi162 – 164Combined sources3
Helixi172 – 175Combined sources4
Beta strandi178 – 187Combined sources10

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1Z24X-ray2.60A1-189[»]
ProteinModelPortaliP00305.
SMRiP00305.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP00305.

Family & Domainsi

Domaini

The molecule consist primarily of eight antiparallel beta-pleated strands, which enclose a hydrophobic pocket, and an alpha-helix.

Sequence similaritiesi

Belongs to the calycin superfamily. Lipocalin family.Curated

Family and domain databases

Gene3Di2.40.128.20. 1 hit.
InterProiIPR012674. Calycin.
IPR011038. Calycin-like.
IPR003057. Invtbrt_color.
IPR022271. Lipocalin_ApoD.
IPR022272. Lipocalin_CS.
IPR000566. Lipocln_cytosolic_FA-bd_dom.
[Graphical view]
PfamiPF00061. Lipocalin. 1 hit.
[Graphical view]
PIRSFiPIRSF036893. Lipocalin_ApoD. 1 hit.
PRINTSiPR01273. INVTBRTCOLOR.
SUPFAMiSSF50814. SSF50814. 1 hit.
PROSITEiPS00213. LIPOCALIN. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P00305-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
GDIFYPGYCP DVKPVNDFDL SAFAGAWHEI AKLPLENENQ GKCTIAEYKY
60 70 80 90 100
DGKKASVYNS FVSNGVKEYM EGDLEIAPDA KYTKQGKYVM TFKFGQRVVN
110 120 130 140 150
LVPWVLATDY KNYAINYNCD YHPDKKAHSI HAWILSKSKV LEGNTKEVVD
160 170 180
NVLKTFSHLI DASKFISNDF SEAACQYSTT YSLTGPDRH
Length:189
Mass (Da):21,379
Last modified:July 21, 1986 - v1
Checksum:i4907E45DEB72873D
GO

Sequence databases

PIRiA03225. CUWOI.

Cross-referencesi

Sequence databases

PIRiA03225. CUWOI.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1Z24X-ray2.60A1-189[»]
ProteinModelPortaliP00305.
SMRiP00305.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Miscellaneous databases

EvolutionaryTraceiP00305.

Family and domain databases

Gene3Di2.40.128.20. 1 hit.
InterProiIPR012674. Calycin.
IPR011038. Calycin-like.
IPR003057. Invtbrt_color.
IPR022271. Lipocalin_ApoD.
IPR022272. Lipocalin_CS.
IPR000566. Lipocln_cytosolic_FA-bd_dom.
[Graphical view]
PfamiPF00061. Lipocalin. 1 hit.
[Graphical view]
PIRSFiPIRSF036893. Lipocalin_ApoD. 1 hit.
PRINTSiPR01273. INVTBRTCOLOR.
SUPFAMiSSF50814. SSF50814. 1 hit.
PROSITEiPS00213. LIPOCALIN. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiICYA_MANSE
AccessioniPrimary (citable) accession number: P00305
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: July 21, 1986
Last modified: November 2, 2016
This is version 94 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Keywords - Technical termi

3D-structure, Direct protein sequencing

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.