P00304 (MPAA3_AMBEL) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names	Recommended name: Pollen allergen Amb a 3 Alternative name(s): Allergen Amb a III Allergen Ra3 Allergen=Amb a 3
Organism	Ambrosia artemisiifolia var. elatior (Short ragweed) (Ambrosia elatior)
Taxonomic identifier	4215 [NCBI]
Taxonomic lineage	cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › asterids › campanulids › Asterales › Asteraceae › Asteroideae › Heliantheae alliance › Heliantheae › Ambrosia › Ambrosia artemisiifolia

Protein attributes

Sequence length	101 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Post-translational modification	The identity of the saccharide is not reported in Ref.1. The sugar attached to Ser-84 is represented simply as Hex. Cys-97 sulfhydryl group is modified but does not form an interchain disulfide bond.
Allergenic properties	Causes an allergic reaction in human.
Sequence similarities	Contains 1 phytocyanin domain.

Ontologies

Keywords
Disease	Allergen
PTM	Disulfide bond Glycoprotein
Technical term	Direct protein sequencing
Gene Ontology (GO)
Molecular_function	copper ion binding Inferred from electronic annotation. Source: InterPro electron carrier activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 101	101	Pollen allergen Amb a 3		PRO_0000085558
Regions
	Domain	2 – 101	100	Phytocyanin
Amino acid modifications
	Modified residue	97	1	Cysteine derivative
	Modified residue	101	1	Blocked carboxyl end (Arg)
	Glycosylation	41	1	N-linked (GlcNAc...) Ref.1
	Glycosylation	84	1	O-linked (Hex) Probable Ref.1
	Disulfide bond	61 ↔ 88		Probable

Sequences

	Sequence		Length	Mass (Da)
	P00304 [UniParc]. Last modified October 1, 1993. Version 2. Checksum: 3CBD6721B85C01DD	FASTA	101	11,375
	10 20 30 40 50 60 GKVYLVGGPE LGGWKLQSDP RAYALWSARQ QFKTTDVLWF NFTTGEDSVA EVWREEAYHA 70 80 90 100 CDIKDPIRLE PGGPDRFTLL TPGSHFICTK DQKFVACVPG R

References

[1]	"Amino acid sequence of ragweed allergen Ra3." Klapper D.G., Goodfriend L., Capra J.D. Biochemistry 19:5729-5734(1980) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE, POST-TRANSLATIONAL MODIFICATIONS. Tissue: Pollen.

Cross-references

Sequence databases
PIR	ARRA3. A00313.
3D structure databases
ProteinModelPortal	P00304.
ModBase	Search...
Protein family/group databases
Allergome	27. Amb a 3. 3068. Amb a 3.0101.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
Gene3D	2.60.40.420. 1 hit.
InterPro	IPR008972. Cupredoxin. IPR003245. Plcyanin-like. [Graphical view]
Pfam	PF02298. Cu_bind_like. 1 hit. [Graphical view]
ProDom	PD003122. Plcyanin-like. 1 hit. [Graphical view] [Entries sharing at least one domain]
SUPFAM	SSF49503. Cupredoxin. 1 hit.
PROSITE	PS00196. COPPER_BLUE. False negative. PS51485. PHYTOCYANIN. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MPAA3_AMBEL

Accession

Primary (citable) accession number: P00304

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 21, 1986
Last sequence update:	October 1, 1993
Last modified:	April 3, 2013
This is version 69 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program