P00280 (AZUR_ALCDE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Azurin

Gene names

Name:

azu

Organism

Alcaligenes denitrificans (Achromobacter denitrificans)

Taxonomic identifier

32002 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Alcaligenaceae › Achromobacter

Protein attributes

Sequence length	149 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Transfers electrons from cytochrome c551 to cytochrome oxidase.
Subcellular location	Periplasm.
Sequence similarities	Contains 1 plastocyanin-like domain.

Ontologies

Keywords
Biological process	Electron transport Transport
Cellular component	Periplasm
Domain	Signal
Ligand	Copper Metal-binding
PTM	Disulfide bond
Technical term	3D-structure Direct protein sequencing
Gene Ontology (GO)
Biological process	electron transport chain Inferred from electronic annotation. Source: UniProtKB-KW transport Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	periplasmic space Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	copper ion binding Inferred from electronic annotation. Source: InterPro electron carrier activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Signal peptide

1 – 20

20

Ref.2

Chain

21 – 149

129

Azurin

PRO_0000002858

Regions

Domain

21 – 149

129

Plastocyanin-like

Sites

Metal binding

66

1

Copper Ref.5

Metal binding

132

1

Copper Ref.5

Metal binding

137

1

Copper Ref.5

Metal binding

141

1

Copper Ref.5

Amino acid modifications

Disulfide bond

23 ↔ 46

Experimental info

Sequence conflict

77

1

Q → E AA sequence Ref.2

Secondary structure

1

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149

Helix Strand Turn

Details...

Beta strand

23 – 29

7

Beta strand

31 – 33

3

Beta strand

38 – 43

6

Beta strand

47 – 55

9

Helix

61 – 64

4

Beta strand

69 – 72

4

Helix

73 – 75

3

Helix

76 – 86

11

Helix

88 – 90

3

Beta strand

101 – 103

3

Beta strand

112 – 118

7

Helix

119 – 121

3

Beta strand

128 – 131

4

Turn

135 – 140

6

Beta strand

141 – 148

8

Sequences

	Sequence		Length	Mass (Da)
	P00280 [UniParc]. Last modified November 1, 1990. Version 2. Checksum: 849F182DD6B1BE3B	FASTA	149	15,924
	10 20 30 40 50 60 MLAKATLAIV LSAASLPVLA AQCEATIESN DAMQYNLKEM VVDKSCKQFT VHLKHVGKMA 70 80 90 100 110 120 KVAMGHNWVL TKEADKQGVA TDGMNAGLAQ DYVKAGDTRV IAHTKVIGGG ESDSVTFDVS 130 140 KLTPGEAYAY FCSFPGHWAM MKGTLKLSN

References

[1]	"Isolation and sequencing of the Alcaligenes denitrificans azurin-encoding gene: comparison with the genes encoding blue copper proteins from Pseudomonas aeruginosa and Alcaligenes faecalis." Hoitink C.W.G., Woudt L.P., Turenhout J.C.M., van de Kamp M., Canters G.W. Gene 90:15-20(1990) [PubMed: 2116366] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	Ambler R.P. Submitted (DEC-1974) to the PIR data bank Cited for: PROTEIN SEQUENCE OF 21-149. Strain: ATCC 15173 / DSM 30026 / JCM 5490 / NBRC 15125 / NCIMB 11961 / NCTC 8582.
[3]	Ambler R.P. (In) Preverio A., Pechere J.-F., Coletti-preverio M.-A. (eds.); Developpements recents dans l'etude chimique de la structure des proteines, pp.289-305, INSERM, Paris (1971) Cited for: PARTIAL PROTEIN SEQUENCE.
[4]	"Structure of azurin from Alcaligenes denitrificans at 2.5-A resolution." Norris G.E., Anderson B.F., Baker E.N. J. Mol. Biol. 165:501-521(1983) [PubMed: 6842609] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.5 ANGSTROMS).
[5]	"Structure of azurin from Alcaligenes denitrificans refinement at 1.8-A resolution and comparison of the two crystallographically independent molecules." Baker E.N. J. Mol. Biol. 203:1071-1095(1988) [PubMed: 3210236] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.8 ANGSTROMS).
[6]	"X-ray analysis and spectroscopic characterization of M121Q azurin. A copper site model for stellacyanin." Romero A., Hoitink C.W., Nar H., Huber R., Messerschmidt A., Canters G.W. J. Mol. Biol. 229:1007-1021(1993) [PubMed: 8383207] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.94 ANGSTROMS) OF GLN-141.
[7]	"Paramagnetic cobalt and nickel derivatives of Alcaligenes denitrificans azurin and its M121Q mutant. A 1H NMR study." Kroes S., Wamerdam G.C.M., Canters G.W. Biochemistry 35:1810-1819(1996) [PubMed: 8639662] [Abstract] Cited for: STRUCTURE BY NMR.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

M30388 Genomic DNA. Translation: AAA21954.1.

PIR

AZALCD. JQ0643.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1A4A	X-ray	1.89	A/B	21-149	[»]
1A4B	X-ray	1.91	A/B	21-149	[»]
1A4C	X-ray	2.45	A/B/C/D	21-149	[»]
1AIZ	X-ray	1.80	A/B	21-149	[»]
1AZB	X-ray	2.20	A/B	21-149	[»]
1AZC	X-ray	1.80	A/B	21-149	[»]
1URI	X-ray	1.94	A/B	21-149	[»]
2AZA	X-ray	1.80	A/B	21-149	[»]

ProteinModelPortal

P00280.

SMR

P00280. Positions 21-149.

ModBase

Search...

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Family and domain databases

InterPro

IPR014068. Azurin_proteobac.
IPR000923. BlueCu_1.
IPR008972. Cupredoxin.
[Graphical view]

Gene3D

G3DSA:2.60.40.420. Cupredoxin. 1 hit.

Pfam

PF00127. Copper-bind. 1 hit.
[Graphical view]

SUPFAM

SSF49503. Cupredoxin. 1 hit.

TIGRFAMs

TIGR02695. Azurin. 1 hit.

PROSITE

PS00196. COPPER_BLUE. 1 hit.
[Graphical view]

ProtoNet

Search...

Entry information

Entry name

AZUR_ALCDE

Accession

Primary (citable) accession number: P00280

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 21, 1986
Last sequence update:	November 1, 1990
Last modified:	May 31, 2011
This is version 84 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program