P00270 (RUBR_DESGI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names	Recommended name: Rubredoxin Short name=Rd
Organism	Desulfovibrio gigas
Taxonomic identifier	879 [NCBI]
Taxonomic lineage	cellular organisms › Bacteria › Proteobacteria › delta/epsilon subdivisions › Deltaproteobacteria › Desulfovibrionales › Desulfovibrionaceae › Desulfovibrio

Protein attributes

Sequence length	52 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Rubredoxin is a small nonheme, iron protein lacking acid-labile sulfide. Its single Fe, chelated to 4 Cys, functions as an electron acceptor and may also stabilize the conformation of the molecule. Electron acceptor for cytoplasmic lactate dehydrogenase.
Cofactor	Binds 1 Fe3+ ion per subunit.
Subcellular location	Cytoplasm.
Sequence similarities	Belongs to the rubredoxin family. Contains 1 rubredoxin-like domain.

Ontologies

Keywords
Biological process	Electron transport Transport
Cellular component	Cytoplasm
Ligand	Iron Metal-binding
PTM	Formylation
Technical term	3D-structure Direct protein sequencing
Gene Ontology (GO)
Biological_process	electron transport chain Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	electron carrier activity Inferred from electronic annotation. Source: InterPro iron ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 52

52

Rubredoxin

PRO_0000135037

Regions

Domain

1 – 52

52

Rubredoxin-like

Sites

Metal binding

6

1

Iron Ref.1

Metal binding

9

1

Iron Ref.1

Metal binding

39

1

Iron Ref.1

Metal binding

42

1

Iron Ref.1

Amino acid modifications

Modified residue

1

1

N-formylmethionine Ref.2

Secondary structure

1

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

52

Helix Strand Turn

Details...

Beta strand

4 – 6

3

Turn

7 – 9

3

Turn

15 – 17

3

Helix

20 – 22

3

Helix

30 – 32

3

Turn

40 – 42

3

Helix

46 – 48

3

Beta strand

49 – 51

3

Sequences

	Sequence		Length	Mass (Da)
	P00270 [UniParc]. Last modified July 21, 1986. Version 1. Checksum: 3E08147A4AC262F7	FASTA	52	5,677
	10 20 30 40 50 MDIYVCTVCG YEYDPAKGDP DSGIKPGTKF EDLPDDWACP VCGASKDAFE KQ

References

[1]	"The amino acid sequence of rubredoxin from the sulfate reducing bacterium, Desulfovibrio gigas." Bruschi M. Biochem. Biophys. Res. Commun. 70:615-621(1976) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE.
[2]	"Rubredoxin from Desulfovibrio gigas. A molecular model of the oxidized form at 1.4-A resolution." Frey M., Sieker L.C., Payan F., Haser R., Bruschi M., Pepe G., le Gall J. J. Mol. Biol. 197:525-541(1987) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.4 ANGSTROMS).
+	Additional computationally mapped references.

Cross-references

Sequence databases

PIR

RUDVEG. A00275.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1E8J	NMR	-	A	1-52	[»]
1RDG	X-ray	1.40	A	1-52	[»]
1SPW	NMR	-	A	1-52	[»]
2DSX	X-ray	0.68	A	1-52	[»]

ProteinModelPortal

P00270.

SMR

P00270. Positions 1-52.

ModBase

Search...

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Family and domain databases

Gene3D

2.20.28.10. 1 hit.

InterPro

IPR024922. Rubredoxin.
IPR024934. Rubredoxin-like_dom.
IPR004039. Rubredoxin-type_fold.
IPR024935. Rubredoxin_dom.
IPR018527. Rubredoxin_Fe_BS.
[Graphical view]

Pfam

PF00301. Rubredoxin. 1 hit.
[Graphical view]

PIRSF

PIRSF000071. Rubredoxin. 1 hit.

PRINTS

PR00163. RUBREDOXIN.

PROSITE

PS00202. RUBREDOXIN. 1 hit.
PS50903. RUBREDOXIN_LIKE. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

EvolutionaryTrace

P00270.

Entry information

Entry name

RUBR_DESGI

Accession

Primary (citable) accession number: P00270

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 21, 1986
Last sequence update:	July 21, 1986
Last modified:	April 3, 2013
This is version 85 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program