P00268 (RUBR_CLOPA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names	Recommended name: Rubredoxin Short name=Rd
Organism	Clostridium pasteurianum
Taxonomic identifier	1501 [NCBI]
Taxonomic lineage	Bacteria › Firmicutes › Clostridia › Clostridiales › Clostridiaceae › Clostridium

Protein attributes

Sequence length	54 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Rubredoxin is a small nonheme, iron protein lacking acid-labile sulfide. Its single Fe, chelated to 4 Cys, functions as an electron acceptor and may also stabilize the conformation of the molecule.
Cofactor	Binds 1 Fe3+ ion per subunit.
Sequence similarities	Belongs to the rubredoxin family. Contains 1 rubredoxin-like domain.

Ontologies

Keywords
Biological process	Electron transport Transport
Ligand	Iron Metal-binding
PTM	Formylation
Technical term	3D-structure Direct protein sequencing
Gene Ontology (GO)
Biological process	electron transport chain Inferred from electronic annotation. Source: UniProtKB-KW transport Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	electron carrier activity Inferred from electronic annotation. Source: InterPro metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 54

54

Rubredoxin

PRO_0000135030

Regions

Domain

1 – 54

54

Rubredoxin-like

Sites

Metal binding

6

1

Iron Ref.5

Metal binding

9

1

Iron Ref.5

Metal binding

39

1

Iron Ref.5

Metal binding

42

1

Iron Ref.5

Amino acid modifications

Modified residue

1

1

N-formylmethionine Ref.1

Secondary structure

1

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

54

Helix Strand Turn

Details...

Beta strand

4 – 6

3

Turn

7 – 9

3

Turn

15 – 17

3

Helix

20 – 22

3

Helix

30 – 32

3

Turn

40 – 42

3

Helix

46 – 48

3

Beta strand

49 – 51

3

Sequences

	Sequence		Length	Mass (Da)
	P00268 [UniParc]. Last modified November 1, 1991. Version 2. Checksum: 81A0826C56A05766	FASTA	54	6,048
	10 20 30 40 50 MKKYTCTVCG YIYNPEDGDP DNGVNPGTDF KDIPDDWVCP LCGVGKDQFE EVEE

References

[1]	"Cloning, sequencing and expression in Escherichia coli of the rubredoxin gene from Clostridium pasteurianum." Mathieu I., Meyer J., Moulis J.-M. Biochem. J. 285:255-262(1992) [PubMed: 1637309] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PARTIAL PROTEIN SEQUENCE.
[2]	McCarthy K.F. Thesis (1972), George Washington University, United States Cited for: PRELIMINARY PROTEIN SEQUENCE.
[3]	"Refinement of the model of a protein, rubredoxin at 1.5-A resolution." Watenpaugh K.D., Siecker L.C., Herriott J.R., Jensen L.H. Acta Crystallogr. B 29:943-956(1973) Cited for: X-RAY CRYSTALLOGRAPHY (1.5 ANGSTROMS).
[4]	"Crystallographic refinement of rubredoxin at 1.2-A resolution." Watenpaugh K.D., Siecker L.C., Jensen L.H. J. Mol. Biol. 138:615-633(1980) [PubMed: 7411618] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.2 ANGSTROMS).
[5]	"Rubredoxin from Clostridium pasteurianum. Structures of G10A, G43A and G10VG43A mutant proteins. Mutation of conserved glycine 10 to valine causes the 9-10 peptide link to invert." Maher M.J., Xiao Z., Wilce M.C., Guss J.M., Wedd A.G. Acta Crystallogr. D 55:962-968(1999) [PubMed: 10216292] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.5 ANGSTROMS).
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

M60116 Genomic DNA. Translation: AAA23279.1.

PIR

RUCLEP. S29120.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1B13	X-ray	1.50	A	1-54	[»]
1B2J	X-ray	1.60	A	1-54	[»]
1B2O	X-ray	1.90	A/B	1-54	[»]
1BE7	X-ray	1.65	A	1-54	[»]
1BFY	NMR	-	A	1-54	[»]
1C09	X-ray	1.60	A/B/C	1-54	[»]
1FHH	X-ray	1.50	A	1-54	[»]
1FHM	X-ray	1.50	A	1-54	[»]
1IRN	X-ray	1.20	A	1-54	[»]
1IRO	X-ray	1.10	A	1-54	[»]
1R0F	X-ray	1.60	A	1-54	[»]
1R0G	X-ray	1.60	A	1-54	[»]
1R0H	X-ray	1.70	A	1-54	[»]
1R0I	X-ray	1.50	A	1-54	[»]
1R0J	X-ray	2.00	A	1-54	[»]
1SMM	X-ray	1.36	A	1-54	[»]
1SMU	X-ray	1.43	A	1-54	[»]
1SMW	X-ray	1.38	A	1-54	[»]
1T9O	X-ray	2.00	A/B/C	1-54	[»]
1T9P	X-ray	1.50	A/B/C	1-54	[»]
1T9Q	X-ray	1.80	A	1-54	[»]
2PVE	X-ray	0.79	A/B/C	1-54	[»]
4RXN	X-ray	1.20	A	1-54	[»]
5RXN	X-ray	1.20	A	1-54	[»]

ProteinModelPortal

P00268.

SMR

P00268. Positions 1-54.

ModBase

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Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Family and domain databases

InterPro

IPR024922. Rubredoxin.
IPR024934. Rubredoxin-like_dom.
IPR004039. Rubredoxin-type_fold.
IPR024935. Rubredoxin_dom.
IPR018527. Rubredoxin_Fe_BS.
[Graphical view]

Gene3D

G3DSA:2.20.28.10. Rubredox. 1 hit.

Pfam

PF00301. Rubredoxin. 1 hit.
[Graphical view]

PIRSF

PIRSF000071. Rubredoxin. 1 hit.

PRINTS

PR00163. RUBREDOXIN.

PROSITE

PS00202. RUBREDOXIN. 1 hit.
PS50903. RUBREDOXIN_LIKE. 1 hit.
[Graphical view]

ProtoNet

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Entry information

Entry name

RUBR_CLOPA

Accession

Primary (citable) accession number: P00268

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 21, 1986
Last sequence update:	November 1, 1991
Last modified:	January 25, 2012
This is version 82 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program