P00259 (PUTX_PSEPU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Putidaredoxin
Short name=PDX

Gene names

Name:

camB

Organism

Pseudomonas putida (Arthrobacter siderocapsulatus)

Taxonomic identifier

303 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas

Protein attributes

Sequence length	107 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	The oxidation of camphor by cytochrome P450-CAM requires the participation of a flavoprotein, putidaredoxin reductase, and an iron-sulfur protein, putidaredoxin, to mediate the transfer of electrons from NADH to P450 for oxygen activation.
Cofactor	Binds 1 2Fe-2S cluster.
Subunit structure	Monomer.
Sequence similarities	Belongs to the adrenodoxin/putidaredoxin family. Contains 1 2Fe-2S ferredoxin-type domain.

Ontologies

Keywords
Biological process	Electron transport Transport
Ligand	2Fe-2S Iron Iron-sulfur Metal-binding
Technical term	3D-structure Direct protein sequencing
Gene Ontology (GO)
Biological process	electron transport chain Inferred from electronic annotation. Source: UniProtKB-KW transport Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	2 iron, 2 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW electron carrier activity Inferred from electronic annotation. Source: InterPro metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Initiator methionine

1

1

Removed Ref.3

Chain

2 – 107

106

Putidaredoxin

PRO_0000201162

Regions

Domain

2 – 106

105

2Fe-2S ferredoxin-type

Sites

Metal binding

40

1

Iron-sulfur (2Fe-2S) Ref.11

Metal binding

46

1

Iron-sulfur (2Fe-2S) Ref.11

Metal binding

49

1

Iron-sulfur (2Fe-2S) Ref.11

Metal binding

87

1

Iron-sulfur (2Fe-2S) Ref.11

Experimental info

Sequence conflict

15

1

E → Q AA sequence Ref.3

Secondary structure

1

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

107

Helix Strand Turn

Details...

Beta strand

3 – 7

5

Beta strand

13 – 17

5

Helix

24 – 30

7

Beta strand

42 – 45

4

Beta strand

50 – 53

4

Turn

55 – 60

6

Helix

66 – 72

7

Beta strand

83 – 85

3

Helix

93 – 95

3

Beta strand

98 – 101

4

Sequences

	Sequence		Length	Mass (Da)
	P00259 [UniParc]. Last modified January 23, 2007. Version 3. Checksum: F487E481F8BEE2C9	FASTA	107	11,550
	10 20 30 40 50 60 MSKVVYVSHD GTRRELDVAD GVSLMQAAVS NGIYDIVGDC GGSASCATCH VYVNEAFTDK 70 80 90 100 VPAANEREIG MLECVTAELK PNSRLCCQII MTPELDGIVV DVPDRQW

References

[1]	"Putidaredoxin reductase and putidaredoxin. Cloning, sequence determination, and heterologous expression of the proteins." Peterson J.A., Lorence M.C., Amarneh B. J. Biol. Chem. 265:6066-6073(1990) [PubMed: 2180940] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Cloning and nucleotide sequences of NADH-putidaredoxin reductase gene (camA) and putidaredoxin gene (camB) involved in cytochrome P-450cam hydroxylase of Pseudomonas putida." Koga H., Yamaguchi E., Matsunaga K., Aramaki H., Horiuchi T. J. Biochem. 106:831-836(1989) [PubMed: 2613690] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: G1 / ATCC 17453.
[3]	"The amino acid sequence of putidaredoxin, an iron-sulfur protein from Pseudomonas putida." Tanaka M., Haniu M., Yasunobu K.T., Dus K., Gunsalus I.C. J. Biol. Chem. 249:3689-3701(1974) [PubMed: 4833743] [Abstract] Cited for: PROTEIN SEQUENCE OF 2-107.
[4]	"Laser flash induced electron transfer in P450cam monooxygenase: putidaredoxin reductase-putidaredoxin interaction." Sevrioukova I.F., Hazzard J.T., Tollin G., Poulos T.L. Biochemistry 40:10592-10600(2001) [PubMed: 11524002] [Abstract] Cited for: INTERACTION WITH PUTIDAREDOXIN REDUCTASE.
[5]	"The putidaredoxin reductase-putidaredoxin electron transfer complex: theoretical and experimental studies." Kuznetsov V.Y., Blair E., Farmer P.J., Poulos T.L., Pifferitti A., Sevrioukova I.F. J. Biol. Chem. 280:16135-16142(2005) [PubMed: 15716266] [Abstract] Cited for: INTERACTION WITH PUTIDAREDOXIN REDUCTASE.
[6]	"Crystal structure of putidaredoxin, the [2Fe-2S] component of the P450cam monooxygenase system from Pseudomonas putida." Sevrioukova I.F., Garcia C., Li H., Bhaskar B., Poulos T.L. J. Mol. Biol. 333:377-392(2003) [PubMed: 14529624] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.47 ANGSTROMS) OF MUTANTS.
[7]	"Structure of C73G putidaredoxin from Pseudomonas putida." Smith N., Mayhew M., Holden M.J., Kelly H., Robinson H., Heroux A., Vilker V.L., Gallagher D.T. Acta Crystallogr. D 60:816-822(2004) [PubMed: 15103126] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.9 ANGSTROMS) OF MUTANT GLY-74.
[8]	"Redox-dependent structural reorganization in putidaredoxin, a vertebrate-type [2Fe-2S] ferredoxin from Pseudomonas putida." Sevrioukova I.F. J. Mol. Biol. 347:607-621(2005) [PubMed: 15755454] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.45 ANGSTROMS) OF MUTANTS SER-74 AND SER-86.
[9]	"1H NMR identification of a beta-sheet structure and description of folding topology in putidaredoxin." Pochapsky T.C., Ye X.M. Biochemistry 30:3850-3856(1991) [PubMed: 2018758] [Abstract] Cited for: STRUCTURE BY NMR.
[10]	"1H NMR sequential assignments and identification of secondary structural elements in oxidized putidaredoxin, an electron-transfer protein from Pseudomonas." Ye X.M., Pochapsky T.C., Pochapsky S.S. Biochemistry 31:1961-1968(1992) [PubMed: 1536837] [Abstract] Cited for: STRUCTURE BY NMR.
[11]	"An NMR-derived model for the solution structure of oxidized putidaredoxin, a 2-Fe, 2-S ferredoxin from Pseudomonas." Pochapsky T.C., Ye X.M., Ratnaswamy G., Lyons T.A. Biochemistry 33:6424-6432(1994) [PubMed: 8204575] [Abstract] Cited for: STRUCTURE BY NMR.
[12]	"Redox-dependent 1H NMR spectral features and tertiary structural constraints on the C-terminal region of putidaredoxin." Pochapsky T.C., Ratnaswamy G., Patera A. Biochemistry 33:6433-6441(1994) [PubMed: 8204576] [Abstract] Cited for: STRUCTURE BY NMR.
[13]	"The solution structure of a gallium-substituted putidaredoxin mutant: GaPdx C85S." Pochapsky T.C., Kuti M., Kazanis S. J. Biomol. NMR 12:407-415(1998) [PubMed: 9835048] [Abstract] Cited for: STRUCTURE BY NMR.
[14]	"A refined model for the solution structure of oxidized putidaredoxin." Pochapsky T.C., Jain N.U., Kuti M., Lyons T.A., Heymont J. Biochemistry 38:4681-4690(1999) [PubMed: 10200155] [Abstract] Cited for: STRUCTURE BY NMR.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

J05406 Genomic DNA. Translation: AAA25759.1.
D00528 Genomic DNA. Translation: BAA00414.1.

PIR

PXPSEP. JX0079.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1GPX	NMR	-	A	2-107	[»]
1OQQ	X-ray	1.47	A/B	2-107	[»]
1OQR	X-ray	1.65	A/B/C	2-107	[»]
1PDX	NMR	-	A	2-107	[»]
1PUT	NMR	-	A	2-107	[»]
1R7S	X-ray	1.91	A/B/C	2-107	[»]
1XLN	X-ray	2.03	A/B	2-107	[»]
1XLO	X-ray	1.84	A/B	2-107	[»]
1XLP	X-ray	2.00	A/B/C	2-107	[»]
1XLQ	X-ray	1.45	A/B/C	2-107	[»]
1YJI	NMR	-	A	2-106	[»]
1YJJ	NMR	-	A	2-106	[»]
3LB8	X-ray	2.60	C/D	2-107	[»]

ProteinModelPortal

P00259.

SMR

P00259. Positions 2-107.

ModBase

Search...

Protein-protein interaction databases

DIP

DIP-29810N.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Enzyme and pathway databases

BioCyc

MetaCyc:MONOMER-3502.

Family and domain databases

InterPro

IPR001055. Adrenodoxin.
IPR018298. Adrenodoxin_Fe-S_BS.
IPR012675. Beta-grasp_ferredoxin-type.
IPR001041. Ferredoxin.
[Graphical view]

Gene3D

G3DSA:3.10.20.30. Ferredoxin_fold. 1 hit.

Pfam

PF00111. Fer2. 1 hit.
[Graphical view]

PRINTS

PR00355. ADRENODOXIN.

SUPFAM

SSF54292. Ferredoxin. 1 hit.

PROSITE

PS51085. 2FE2S_FER_2. 1 hit.
PS00814. ADX. 1 hit.
[Graphical view]

ProtoNet

Search...

Entry information

Entry name

PUTX_PSEPU

Accession

Primary (citable) accession number: P00259

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 21, 1986
Last sequence update:	January 23, 2007
Last modified:	May 31, 2011
This is version 94 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program