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P00214

- FER1_AZOVI

UniProt

P00214 - FER1_AZOVI

Protein

Ferredoxin-1

Gene

fdxA

Organism
Azotobacter vinelandii
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
  1. Functioni

    Ferredoxins are iron-sulfur proteins that transfer electrons in a wide variety of metabolic reactions. This ferredoxin could play a role in regulating gene expression by interacting directly with DNA.

    Cofactori

    Binds 1 4Fe-4S cluster.
    Binds 1 3Fe-4S cluster.

    Redox potential

    E0 is -450 mV for the 3Fe-4S, and -645 mV for the 4Fe-4S clusters.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi9 – 91Iron-sulfur 1 (3Fe-4S)1 Publication
    Metal bindingi17 – 171Iron-sulfur 1 (3Fe-4S)1 Publication
    Metal bindingi21 – 211Iron-sulfur 2 (4Fe-4S)1 Publication
    Metal bindingi40 – 401Iron-sulfur 2 (4Fe-4S)1 Publication
    Metal bindingi43 – 431Iron-sulfur 2 (4Fe-4S)1 Publication
    Metal bindingi46 – 461Iron-sulfur 2 (4Fe-4S)1 Publication
    Metal bindingi50 – 501Iron-sulfur 1 (3Fe-4S)1 Publication

    GO - Molecular functioni

    1. 3 iron, 4 sulfur cluster binding Source: UniProtKB-KW
    2. 4 iron, 4 sulfur cluster binding Source: UniProtKB-KW
    3. DNA binding Source: UniProtKB-KW
    4. electron carrier activity Source: InterPro
    5. metal ion binding Source: UniProtKB-KW

    GO - Biological processi

    1. oxidation-reduction process Source: UniProtKB-KW

    Keywords - Biological processi

    Electron transport, Transport

    Keywords - Ligandi

    3Fe-4S, 4Fe-4S, DNA-binding, Iron, Iron-sulfur, Metal-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Ferredoxin-1
    Alternative name(s):
    Ferredoxin I
    Short name:
    FdI
    Gene namesi
    Name:fdxA
    OrganismiAzotobacter vinelandii
    Taxonomic identifieri354 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaeAzotobacter

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11Removed1 Publication
    Chaini2 – 107106Ferredoxin-1PRO_0000159095Add
    BLAST

    Structurei

    Secondary structure

    1
    107
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi3 – 53
    Helixi7 – 93
    Turni10 – 123
    Helixi16 – 205
    Beta strandi22 – 243
    Beta strandi26 – 283
    Beta strandi33 – 353
    Turni37 – 393
    Helixi47 – 493
    Beta strandi55 – 573
    Helixi58 – 603
    Helixi63 – 664
    Helixi67 – 759
    Turni76 – 783
    Helixi92 – 954
    Helixi101 – 1044

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1A6LX-ray2.10A2-107[»]
    1AXQX-ray2.10A2-107[»]
    1B0TX-ray2.10A2-107[»]
    1B0VX-ray2.80A/B/C/D2-107[»]
    1D3WX-ray1.70A2-107[»]
    1F5BX-ray1.62A2-107[»]
    1F5CX-ray1.75A2-107[»]
    1FD2X-ray1.90A2-107[»]
    1FDAX-ray2.10A2-107[»]
    1FDBX-ray2.20A2-107[»]
    1FDDX-ray1.90A2-107[»]
    1FERX-ray2.30A2-107[»]
    1FF2X-ray2.30A2-107[»]
    1FRHX-ray2.30A2-107[»]
    1FRIX-ray2.10A2-107[»]
    1FRJX-ray2.30A2-107[»]
    1FRKX-ray2.10A2-107[»]
    1FRLX-ray2.30A2-107[»]
    1FRMX-ray2.30A2-107[»]
    1FRXX-ray2.50A2-107[»]
    1FTCX-ray2.35A/B2-107[»]
    1G3OX-ray1.65A2-107[»]
    1G6BX-ray1.90A2-107[»]
    1GAOX-ray2.20A/B/C/D2-107[»]
    1PC4X-ray1.65A1-107[»]
    1PC5X-ray1.80A1-107[»]
    2FD2X-ray1.90A2-107[»]
    5FD1X-ray1.90A2-107[»]
    6FD1X-ray1.35A2-107[»]
    6FDRX-ray1.40A2-107[»]
    7FD1X-ray1.30A2-107[»]
    7FDRX-ray1.40A2-107[»]
    ProteinModelPortaliP00214.
    SMRiP00214. Positions 2-107.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP00214.

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini2 – 30294Fe-4S ferredoxin-type 1PROSITE-ProRule annotationAdd
    BLAST
    Domaini31 – 60304Fe-4S ferredoxin-type 2PROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Contains 2 4Fe-4S ferredoxin-type domains.PROSITE-ProRule annotation

    Keywords - Domaini

    Repeat

    Family and domain databases

    InterProiIPR001450. 4Fe4S-bd_dom.
    IPR017896. 4Fe4S_Fe-S-bd.
    IPR017900. 4Fe4S_Fe_S_CS.
    IPR000813. 7Fe_ferredoxin.
    IPR022569. Fd_C.
    [Graphical view]
    PfamiPF11953. DUF3470. 1 hit.
    PF00037. Fer4. 1 hit.
    [Graphical view]
    PRINTSiPR00354. 7FE8SFRDOXIN.
    PROSITEiPS00198. 4FE4S_FER_1. 1 hit.
    PS51379. 4FE4S_FER_2. 2 hits.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P00214-1 [UniParc]FASTAAdd to Basket

    « Hide

    MAFVVTDNCI KCKYTDCVEV CPVDCFYEGP NFLVIHPDEC IDCALCEPEC    50
    PAQAIFSEDE VPEDMQEFIQ LNAELAEVWP NITEKKDPLP DAEDWDGVKG 100
    KLQHLER 107
    Length:107
    Mass (Da):12,182
    Last modified:January 23, 2007 - v2
    Checksum:i3B4B0D1A55765B2B
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti49 – 491E → R in AAA16869. (PubMed:8244942)Curated
    Sequence conflicti53 – 531Q → E AA sequence (PubMed:6848518)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    J03521 Genomic DNA. Translation: AAA22125.1.
    M63007 Unassigned DNA. Translation: AAA16869.1.
    PIRiA29936. FEAV.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    J03521 Genomic DNA. Translation: AAA22125.1 .
    M63007 Unassigned DNA. Translation: AAA16869.1 .
    PIRi A29936. FEAV.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1A6L X-ray 2.10 A 2-107 [» ]
    1AXQ X-ray 2.10 A 2-107 [» ]
    1B0T X-ray 2.10 A 2-107 [» ]
    1B0V X-ray 2.80 A/B/C/D 2-107 [» ]
    1D3W X-ray 1.70 A 2-107 [» ]
    1F5B X-ray 1.62 A 2-107 [» ]
    1F5C X-ray 1.75 A 2-107 [» ]
    1FD2 X-ray 1.90 A 2-107 [» ]
    1FDA X-ray 2.10 A 2-107 [» ]
    1FDB X-ray 2.20 A 2-107 [» ]
    1FDD X-ray 1.90 A 2-107 [» ]
    1FER X-ray 2.30 A 2-107 [» ]
    1FF2 X-ray 2.30 A 2-107 [» ]
    1FRH X-ray 2.30 A 2-107 [» ]
    1FRI X-ray 2.10 A 2-107 [» ]
    1FRJ X-ray 2.30 A 2-107 [» ]
    1FRK X-ray 2.10 A 2-107 [» ]
    1FRL X-ray 2.30 A 2-107 [» ]
    1FRM X-ray 2.30 A 2-107 [» ]
    1FRX X-ray 2.50 A 2-107 [» ]
    1FTC X-ray 2.35 A/B 2-107 [» ]
    1G3O X-ray 1.65 A 2-107 [» ]
    1G6B X-ray 1.90 A 2-107 [» ]
    1GAO X-ray 2.20 A/B/C/D 2-107 [» ]
    1PC4 X-ray 1.65 A 1-107 [» ]
    1PC5 X-ray 1.80 A 1-107 [» ]
    2FD2 X-ray 1.90 A 2-107 [» ]
    5FD1 X-ray 1.90 A 2-107 [» ]
    6FD1 X-ray 1.35 A 2-107 [» ]
    6FDR X-ray 1.40 A 2-107 [» ]
    7FD1 X-ray 1.30 A 2-107 [» ]
    7FDR X-ray 1.40 A 2-107 [» ]
    ProteinModelPortali P00214.
    SMRi P00214. Positions 2-107.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Miscellaneous databases

    EvolutionaryTracei P00214.

    Family and domain databases

    InterProi IPR001450. 4Fe4S-bd_dom.
    IPR017896. 4Fe4S_Fe-S-bd.
    IPR017900. 4Fe4S_Fe_S_CS.
    IPR000813. 7Fe_ferredoxin.
    IPR022569. Fd_C.
    [Graphical view ]
    Pfami PF11953. DUF3470. 1 hit.
    PF00037. Fer4. 1 hit.
    [Graphical view ]
    PRINTSi PR00354. 7FE8SFRDOXIN.
    PROSITEi PS00198. 4FE4S_FER_1. 1 hit.
    PS51379. 4FE4S_FER_2. 2 hits.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Azotobacter vinelandii ferredoxin I: cloning, sequencing, and mutant analysis."
      Morgan T.V., Lundell D.J., Burgess B.K.
      J. Biol. Chem. 263:1370-1375(1988) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. "Azotobacter vinelandii mutS: nucleotide sequence and mutant analysis."
      Le O., Shen B., Iismaa S.E., Burgess B.K.
      J. Bacteriol. 175:7707-7710(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 13705 / OP1 / DSM 366 / NCIB 11614 / LMG 3878 / UW.
    3. "Structure of Azotobacter vinelandii 7Fe ferredoxin. Amino acid sequence and electron density maps of residues."
      Howard J.B., Lorsbach T.W., Ghosh D., Melis K., Stout C.D.
      J. Biol. Chem. 258:508-522(1983) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 2-107, X-RAY CRYSTALLOGRAPHY (2 ANGSTROMS).
    4. "Iron-sulfur clusters and protein structure of Azotobacter ferredoxin at 2.0-A resolution."
      Ghosh D., O'Donnell S., Furey W.F. Jr., Robbins A.H., Stout C.D.
      J. Mol. Biol. 158:73-109(1982) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2 ANGSTROMS).
    5. Cited for: X-RAY CRYSTALLOGRAPHY (3 ANGSTROMS).
    6. "Refinement of the 7 Fe ferredoxin from Azotobacter vinelandii at 1.9-A resolution."
      Stout G.H.
      J. Mol. Biol. 205:545-555(1989) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (1.9 ANGSTROMS).
    7. "Structure at pH 6.5 of ferredoxin I from Azotobacter vinelandii at 2.3-A resolution."
      Merritt E.A., Stout G.H., Turley S., Sieker L.C., Jensen L.H., Orme-Johnson W.H.
      Acta Crystallogr. D 49:272-281(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.3 ANGSTROMS).
    8. "Structure of Azotobacter vinelandii 7Fe ferredoxin at 1.35-A resolution and determination of the [Fe-S] bonds with 0.01-A accuracy."
      Stout C.D., Stura E.A., McRee D.E.
      J. Mol. Biol. 278:629-639(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (1.35 ANGSTROMS).
    9. "Oxidized and reduced Azotobacter vinelandii ferredoxin I at 1.4-A resolution: conformational change of surface residues without significant change in the [3Fe-4S]+/0 cluster."
      Schipke C.G., Goodin D.B., McRee D.E., Stout C.D.
      Biochemistry 38:8228-8239(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (1.4 ANGSTROMS).
    10. "Alteration of the reduction potential of the [4Fe-4S](2+/+) cluster of Azotobacter vinelandii ferredoxin I."
      Chen K., Tilley G.J., Sridhar V., Prasad G.S., Stout C.D., Armstrong F.A., Burgess B.K.
      J. Biol. Chem. 274:36479-36487(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.8 ANGSTROMS).
    11. "Does ferredoxin I (Azotobacter) represent a novel class of DNA-binding proteins that regulate gene expression in response to cellular iron(II)?"
      Thomson A.J.
      FEBS Lett. 285:230-236(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: DNA-BINDING.
    12. "Site-directed mutagenesis of Azotobacter vinelandii ferredoxin I: [Fe-S] cluster-driven protein rearrangement."
      Martin A.E., Burgess B.K., Stout C.D., Cash V.L., Dean D.R., Jensen G.M., Stephens P.J.
      Proc. Natl. Acad. Sci. U.S.A. 87:598-602(1990) [PubMed] [Europe PMC] [Abstract]
      Cited for: MUTAGENESIS.
    13. "Site-directed mutagenesis of Azotobacter vinelandii ferredoxin I. Changes in [4Fe-4S] cluster reduction potential and reactivity."
      Iismaa S.E., Vazquez A.E., Jensen G.M., Stephens P.J., Butt J.N., Armstrong F.A., Burgess B.K.
      J. Biol. Chem. 266:21563-21571(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: MUTAGENESIS.
    14. "Azotobacter vinelandii ferredoxin I. Alteration of individual surface charges and the [4FE-4S]2+/+ cluster reduction potential."
      Shen B., Jollie D.R., Stout C.D., Diller T.C., Armstrong F.A., Gorst C.M., la Mar G.N., Stephen P.J., Burgess B.K.
      J. Biol. Chem. 269:8564-8575(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: MUTAGENESIS.

    Entry informationi

    Entry nameiFER1_AZOVI
    AccessioniPrimary (citable) accession number: P00214
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 21, 1986
    Last sequence update: January 23, 2007
    Last modified: October 1, 2014
    This is version 110 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Direct protein sequencing

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3