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P00214

- FER1_AZOVI

UniProt

P00214 - FER1_AZOVI

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Protein

Ferredoxin-1

Gene

fdxA

Organism
Azotobacter vinelandii
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli

Functioni

Ferredoxins are iron-sulfur proteins that transfer electrons in a wide variety of metabolic reactions. This ferredoxin could play a role in regulating gene expression by interacting directly with DNA.

Cofactori

Binds 1 4Fe-4S cluster.
Binds 1 3Fe-4S cluster.

Redox potential

E0 is -450 mV for the 3Fe-4S, and -645 mV for the 4Fe-4S clusters.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi9 – 91Iron-sulfur 1 (3Fe-4S)1 Publication
Metal bindingi17 – 171Iron-sulfur 1 (3Fe-4S)1 Publication
Metal bindingi21 – 211Iron-sulfur 2 (4Fe-4S)1 Publication
Metal bindingi40 – 401Iron-sulfur 2 (4Fe-4S)1 Publication
Metal bindingi43 – 431Iron-sulfur 2 (4Fe-4S)1 Publication
Metal bindingi46 – 461Iron-sulfur 2 (4Fe-4S)1 Publication
Metal bindingi50 – 501Iron-sulfur 1 (3Fe-4S)1 Publication

GO - Molecular functioni

  1. 3 iron, 4 sulfur cluster binding Source: UniProtKB-KW
  2. 4 iron, 4 sulfur cluster binding Source: UniProtKB-KW
  3. DNA binding Source: UniProtKB-KW
  4. electron carrier activity Source: InterPro
  5. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. oxidation-reduction process Source: UniProtKB-KW
Complete GO annotation...

Keywords - Biological processi

Electron transport, Transport

Keywords - Ligandi

3Fe-4S, 4Fe-4S, DNA-binding, Iron, Iron-sulfur, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Ferredoxin-1
Alternative name(s):
Ferredoxin I
Short name:
FdI
Gene namesi
Name:fdxA
OrganismiAzotobacter vinelandii
Taxonomic identifieri354 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaeAzotobacter

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed1 Publication
Chaini2 – 107106Ferredoxin-1PRO_0000159095Add
BLAST

Structurei

Secondary structure

1
107
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi3 – 53
Helixi7 – 93
Turni10 – 123
Helixi16 – 205
Beta strandi22 – 243
Beta strandi26 – 283
Beta strandi33 – 353
Turni37 – 393
Helixi47 – 493
Beta strandi55 – 573
Helixi58 – 603
Helixi63 – 664
Helixi67 – 759
Turni76 – 783
Helixi92 – 954
Helixi101 – 1044

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1A6LX-ray2.10A2-107[»]
1AXQX-ray2.10A2-107[»]
1B0TX-ray2.10A2-107[»]
1B0VX-ray2.80A/B/C/D2-107[»]
1D3WX-ray1.70A2-107[»]
1F5BX-ray1.62A2-107[»]
1F5CX-ray1.75A2-107[»]
1FD2X-ray1.90A2-107[»]
1FDAX-ray2.10A2-107[»]
1FDBX-ray2.20A2-107[»]
1FDDX-ray1.90A2-107[»]
1FERX-ray2.30A2-107[»]
1FF2X-ray2.30A2-107[»]
1FRHX-ray2.30A2-107[»]
1FRIX-ray2.10A2-107[»]
1FRJX-ray2.30A2-107[»]
1FRKX-ray2.10A2-107[»]
1FRLX-ray2.30A2-107[»]
1FRMX-ray2.30A2-107[»]
1FRXX-ray2.50A2-107[»]
1FTCX-ray2.35A/B2-107[»]
1G3OX-ray1.65A2-107[»]
1G6BX-ray1.90A2-107[»]
1GAOX-ray2.20A/B/C/D2-107[»]
1PC4X-ray1.65A1-107[»]
1PC5X-ray1.80A1-107[»]
2FD2X-ray1.90A2-107[»]
5FD1X-ray1.90A2-107[»]
6FD1X-ray1.35A2-107[»]
6FDRX-ray1.40A2-107[»]
7FD1X-ray1.30A2-107[»]
7FDRX-ray1.40A2-107[»]
ProteinModelPortaliP00214.
SMRiP00214. Positions 2-107.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP00214.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini2 – 30294Fe-4S ferredoxin-type 1PROSITE-ProRule annotationAdd
BLAST
Domaini31 – 60304Fe-4S ferredoxin-type 2PROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 2 4Fe-4S ferredoxin-type domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Family and domain databases

InterProiIPR001450. 4Fe4S-bd_dom.
IPR017896. 4Fe4S_Fe-S-bd.
IPR017900. 4Fe4S_Fe_S_CS.
IPR000813. 7Fe_ferredoxin.
IPR022569. Fd_C.
[Graphical view]
PfamiPF11953. DUF3470. 1 hit.
PF00037. Fer4. 1 hit.
[Graphical view]
PRINTSiPR00354. 7FE8SFRDOXIN.
PROSITEiPS00198. 4FE4S_FER_1. 1 hit.
PS51379. 4FE4S_FER_2. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P00214-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MAFVVTDNCI KCKYTDCVEV CPVDCFYEGP NFLVIHPDEC IDCALCEPEC
60 70 80 90 100
PAQAIFSEDE VPEDMQEFIQ LNAELAEVWP NITEKKDPLP DAEDWDGVKG

KLQHLER
Length:107
Mass (Da):12,182
Last modified:January 23, 2007 - v2
Checksum:i3B4B0D1A55765B2B
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti49 – 491E → R in AAA16869. (PubMed:8244942)Curated
Sequence conflicti53 – 531Q → E AA sequence (PubMed:6848518)Curated

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
J03521 Genomic DNA. Translation: AAA22125.1.
M63007 Unassigned DNA. Translation: AAA16869.1.
PIRiA29936. FEAV.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
J03521 Genomic DNA. Translation: AAA22125.1 .
M63007 Unassigned DNA. Translation: AAA16869.1 .
PIRi A29936. FEAV.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1A6L X-ray 2.10 A 2-107 [» ]
1AXQ X-ray 2.10 A 2-107 [» ]
1B0T X-ray 2.10 A 2-107 [» ]
1B0V X-ray 2.80 A/B/C/D 2-107 [» ]
1D3W X-ray 1.70 A 2-107 [» ]
1F5B X-ray 1.62 A 2-107 [» ]
1F5C X-ray 1.75 A 2-107 [» ]
1FD2 X-ray 1.90 A 2-107 [» ]
1FDA X-ray 2.10 A 2-107 [» ]
1FDB X-ray 2.20 A 2-107 [» ]
1FDD X-ray 1.90 A 2-107 [» ]
1FER X-ray 2.30 A 2-107 [» ]
1FF2 X-ray 2.30 A 2-107 [» ]
1FRH X-ray 2.30 A 2-107 [» ]
1FRI X-ray 2.10 A 2-107 [» ]
1FRJ X-ray 2.30 A 2-107 [» ]
1FRK X-ray 2.10 A 2-107 [» ]
1FRL X-ray 2.30 A 2-107 [» ]
1FRM X-ray 2.30 A 2-107 [» ]
1FRX X-ray 2.50 A 2-107 [» ]
1FTC X-ray 2.35 A/B 2-107 [» ]
1G3O X-ray 1.65 A 2-107 [» ]
1G6B X-ray 1.90 A 2-107 [» ]
1GAO X-ray 2.20 A/B/C/D 2-107 [» ]
1PC4 X-ray 1.65 A 1-107 [» ]
1PC5 X-ray 1.80 A 1-107 [» ]
2FD2 X-ray 1.90 A 2-107 [» ]
5FD1 X-ray 1.90 A 2-107 [» ]
6FD1 X-ray 1.35 A 2-107 [» ]
6FDR X-ray 1.40 A 2-107 [» ]
7FD1 X-ray 1.30 A 2-107 [» ]
7FDR X-ray 1.40 A 2-107 [» ]
ProteinModelPortali P00214.
SMRi P00214. Positions 2-107.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Miscellaneous databases

EvolutionaryTracei P00214.

Family and domain databases

InterProi IPR001450. 4Fe4S-bd_dom.
IPR017896. 4Fe4S_Fe-S-bd.
IPR017900. 4Fe4S_Fe_S_CS.
IPR000813. 7Fe_ferredoxin.
IPR022569. Fd_C.
[Graphical view ]
Pfami PF11953. DUF3470. 1 hit.
PF00037. Fer4. 1 hit.
[Graphical view ]
PRINTSi PR00354. 7FE8SFRDOXIN.
PROSITEi PS00198. 4FE4S_FER_1. 1 hit.
PS51379. 4FE4S_FER_2. 2 hits.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Azotobacter vinelandii ferredoxin I: cloning, sequencing, and mutant analysis."
    Morgan T.V., Lundell D.J., Burgess B.K.
    J. Biol. Chem. 263:1370-1375(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "Azotobacter vinelandii mutS: nucleotide sequence and mutant analysis."
    Le O., Shen B., Iismaa S.E., Burgess B.K.
    J. Bacteriol. 175:7707-7710(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 13705 / OP1 / DSM 366 / NCIB 11614 / LMG 3878 / UW.
  3. "Structure of Azotobacter vinelandii 7Fe ferredoxin. Amino acid sequence and electron density maps of residues."
    Howard J.B., Lorsbach T.W., Ghosh D., Melis K., Stout C.D.
    J. Biol. Chem. 258:508-522(1983) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 2-107, X-RAY CRYSTALLOGRAPHY (2 ANGSTROMS).
  4. "Iron-sulfur clusters and protein structure of Azotobacter ferredoxin at 2.0-A resolution."
    Ghosh D., O'Donnell S., Furey W.F. Jr., Robbins A.H., Stout C.D.
    J. Mol. Biol. 158:73-109(1982) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2 ANGSTROMS).
  5. Cited for: X-RAY CRYSTALLOGRAPHY (3 ANGSTROMS).
  6. "Refinement of the 7 Fe ferredoxin from Azotobacter vinelandii at 1.9-A resolution."
    Stout G.H.
    J. Mol. Biol. 205:545-555(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (1.9 ANGSTROMS).
  7. "Structure at pH 6.5 of ferredoxin I from Azotobacter vinelandii at 2.3-A resolution."
    Merritt E.A., Stout G.H., Turley S., Sieker L.C., Jensen L.H., Orme-Johnson W.H.
    Acta Crystallogr. D 49:272-281(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.3 ANGSTROMS).
  8. "Structure of Azotobacter vinelandii 7Fe ferredoxin at 1.35-A resolution and determination of the [Fe-S] bonds with 0.01-A accuracy."
    Stout C.D., Stura E.A., McRee D.E.
    J. Mol. Biol. 278:629-639(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (1.35 ANGSTROMS).
  9. "Oxidized and reduced Azotobacter vinelandii ferredoxin I at 1.4-A resolution: conformational change of surface residues without significant change in the [3Fe-4S]+/0 cluster."
    Schipke C.G., Goodin D.B., McRee D.E., Stout C.D.
    Biochemistry 38:8228-8239(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (1.4 ANGSTROMS).
  10. "Alteration of the reduction potential of the [4Fe-4S](2+/+) cluster of Azotobacter vinelandii ferredoxin I."
    Chen K., Tilley G.J., Sridhar V., Prasad G.S., Stout C.D., Armstrong F.A., Burgess B.K.
    J. Biol. Chem. 274:36479-36487(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.8 ANGSTROMS).
  11. "Does ferredoxin I (Azotobacter) represent a novel class of DNA-binding proteins that regulate gene expression in response to cellular iron(II)?"
    Thomson A.J.
    FEBS Lett. 285:230-236(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: DNA-BINDING.
  12. "Site-directed mutagenesis of Azotobacter vinelandii ferredoxin I: [Fe-S] cluster-driven protein rearrangement."
    Martin A.E., Burgess B.K., Stout C.D., Cash V.L., Dean D.R., Jensen G.M., Stephens P.J.
    Proc. Natl. Acad. Sci. U.S.A. 87:598-602(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: MUTAGENESIS.
  13. "Site-directed mutagenesis of Azotobacter vinelandii ferredoxin I. Changes in [4Fe-4S] cluster reduction potential and reactivity."
    Iismaa S.E., Vazquez A.E., Jensen G.M., Stephens P.J., Butt J.N., Armstrong F.A., Burgess B.K.
    J. Biol. Chem. 266:21563-21571(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: MUTAGENESIS.
  14. "Azotobacter vinelandii ferredoxin I. Alteration of individual surface charges and the [4FE-4S]2+/+ cluster reduction potential."
    Shen B., Jollie D.R., Stout C.D., Diller T.C., Armstrong F.A., Gorst C.M., la Mar G.N., Stephen P.J., Burgess B.K.
    J. Biol. Chem. 269:8564-8575(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: MUTAGENESIS.

Entry informationi

Entry nameiFER1_AZOVI
AccessioniPrimary (citable) accession number: P00214
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: January 23, 2007
Last modified: October 1, 2014
This is version 110 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Direct protein sequencing

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3