Reviewed, UniProtKB/Swiss-Prot P00198 (FER_CLOAC)

Names and origin

Protein names	Recommended name:     Ferredoxin
Organism	Clostridium acidi-urici
Taxonomic identifier	1556 [NCBI]
Taxonomic lineage	Bacteria › Firmicutes › Clostridia › Clostridiales › Clostridiaceae › Clostridium

Protein attributes

Sequence length	55 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Ferredoxins are iron-sulfur proteins that transfer electrons in a wide variety of metabolic reactions.
Cofactor	Binds 2 4Fe-4S clusters.
Sequence similarities	Contains 2 4Fe-4S ferredoxin-type domains.

Ontologies

Keywords
Biological process	Electron transport Transport
Domain	Repeat
Ligand	4Fe-4S Iron Iron-sulfur Metal-binding
Technical term	3D-structure Direct protein sequencing
Gene Ontology (GO)
Biological process	electron transport chain Inferred from electronic annotation. Source: UniProtKB-KW transport Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW electron carrier activity Inferred from electronic annotation. Source: InterPro iron ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 55

55

Ferredoxin

PRO_0000159109

Regions

Domain

2 – 27

26

4Fe-4S ferredoxin-type 1

Domain

28 – 55

28

4Fe-4S ferredoxin-type 2

Sites

Metal binding

8

1

Iron-sulfur 1 (4Fe-4S)

Metal binding

11

1

Iron-sulfur 1 (4Fe-4S)

Metal binding

14

1

Iron-sulfur 1 (4Fe-4S)

Metal binding

18

1

Iron-sulfur 2 (4Fe-4S)

Metal binding

37

1

Iron-sulfur 2 (4Fe-4S)

Metal binding

40

1

Iron-sulfur 2 (4Fe-4S)

Metal binding

43

1

Iron-sulfur 2 (4Fe-4S)

Metal binding

47

1

Iron-sulfur 1 (4Fe-4S)

Secondary structure

1

.

.

.

.

.

.

.

.

.

.

.

.

.

55

Helix Strand Turn

Details...

Beta strand

2 – 4

3

Helix

15 – 17

3

Beta strand

27 – 29

3

Turn

34 – 36

3

Helix

42 – 45

4

Beta strand

52 – 54

3

Sequences

	Sequence		Length	Mass (Da)
	P00198-1 [UniParc]. Last modified December 1, 1992. Version 2. Checksum: D038C1364A1E97E6	FASTA	55	5,539
	10 20 30 40 50 AYVINEACIS CGACEPECPV NAISSGDDRY VIDADTCIDC GACAGVCPVD APVQA

References

[1]	"The amino acid sequence of ferredoxin from Clostridium acidi-urici." Rall S.C., Bolinger R.E., Cole R.D. Biochemistry 8:2486-2496(1969) [PubMed: 5799135] [Abstract] Cited for: PROTEIN SEQUENCE.
[2]	"Sequences of clostridial ferredoxins: determination of the Clostridium sticklandii sequence and correction of the Clostridium acidurici sequence." Meyer J., Moulis J.-M., Scherrer N., Gagnon J., Ulrich J. Biochem. J. 294:622-623(1993) [PubMed: 8373379] [Abstract] Cited for: SEQUENCE REVISION TO 15; 21; 25 AND 28. Strain: ATCC 7906 / CIP 104303 / DSM 604 / NCCB 46094 / 9a.
[3]	"Refined crystal structure of the 2[4Fe-4S] ferredoxin from Clostridium acidurici at 1.84-A resolution." Duee E.D., Fanchon E., Vicat J., Sieker L.C., Meyer J., Moulis J.-M. J. Mol. Biol. 243:683-695(1994) [PubMed: 7966291] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.84 ANGSTROMS).
[4]	"Atomic resolution (0.94 A) structure of Clostridium acidurici ferredoxin. Detailed geometry of [4Fe-4S] clusters in a protein." Dauter Z., Wilson K.S., Sieker L.C., Meyer J., Moulis J.-M. Biochemistry 36:16065-16073(1997) [PubMed: 9405040] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (0.94 ANGSTROMS).
+	Additional computationally mapped references.

Cross-references

Sequence databases

PIR

FECLCU. S36790.

3D structure databases

PDBPDBe

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1FCA	X-ray	1.80	A	1-55	[»]
1FDN	X-ray	1.84	A	1-55	[»]
2FDN	X-ray	0.94	A	1-55	[»]

ModBase

Search...

Family and domain databases

InterPro

IPR017896. 4Fe4S_Fe-S-bd.
IPR001450. 4Fe4S_Fe_S_bd_subgr.
IPR017900. 4Fe4S_Fe_S_CS.
IPR000813. 7Fe_ferredoxin.
[Graphical view]

Pfam

PF00037. Fer4. 2 hits.
[Graphical view]

PRINTS

PR00354. 7FE8SFRDOXIN.

PROSITE

PS00198. 4FE4S_FER_1. 2 hits.
PS51379. 4FE4S_FER_2. 2 hits.
[Graphical view]

ProtoNet

Search...

Entry information

Entry name

FER_CLOAC

Accession

Primary (citable) accession number: P00198

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 21, 1986
Last sequence update:	December 1, 1992
Last modified:	June 16, 2009
This is version 78 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)