P00195 (FER_CLOPA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names	Recommended name: Ferredoxin
Organism	Clostridium pasteurianum
Taxonomic identifier	1501 [NCBI]
Taxonomic lineage	Bacteria › Firmicutes › Clostridia › Clostridiales › Clostridiaceae › Clostridium

Protein attributes

Sequence length	56 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Ferredoxins are iron-sulfur proteins that transfer electrons in a wide variety of metabolic reactions.
Cofactor	Binds 2 4Fe-4S clusters.
Sequence similarities	Contains 2 4Fe-4S ferredoxin-type domains.

Ontologies

Keywords
Biological process	Electron transport Transport
Domain	Repeat
Ligand	4Fe-4S Iron Iron-sulfur Metal-binding
Technical term	3D-structure Direct protein sequencing
Gene Ontology (GO)
Biological process	electron transport chain Inferred from electronic annotation. Source: UniProtKB-KW transport Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW electron carrier activity Inferred from electronic annotation. Source: InterPro metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Initiator methionine

1

1

Removed Ref.2

Chain

2 – 56

55

Ferredoxin

PRO_0000159111

Regions

Domain

2 – 28

27

4Fe-4S ferredoxin-type 1

Domain

29 – 56

28

4Fe-4S ferredoxin-type 2

Sites

Metal binding

9

1

Iron-sulfur 1 (4Fe-4S) Ref.3

Metal binding

12

1

Iron-sulfur 1 (4Fe-4S)

Metal binding

15

1

Iron-sulfur 1 (4Fe-4S)

Metal binding

19

1

Iron-sulfur 2 (4Fe-4S)

Metal binding

38

1

Iron-sulfur 2 (4Fe-4S)

Metal binding

41

1

Iron-sulfur 2 (4Fe-4S)

Metal binding

44

1

Iron-sulfur 2 (4Fe-4S)

Metal binding

48

1

Iron-sulfur 1 (4Fe-4S)

Secondary structure

1

.

.

.

.

.

.

.

.

.

.

.

56

Helix Strand Turn

Details...

Turn

14 – 18

5

Beta strand

24 – 26

3

Beta strand

28 – 33

6

Turn

35 – 37

3

Turn

43 – 47

5

Sequences

	Sequence		Length	Mass (Da)
	P00195 [UniParc]. Last modified January 23, 2007. Version 2. Checksum: D74C5AA8D3E7A4EC	FASTA	56	5,630
	10 20 30 40 50 MAYKIADSCV SCGACASECP VNAISQGDSI FVIDADTCID CGNCANVCPV GAPVQE

References

[1]	"Cloning and nucleotide sequence determination of the Clostridium pasteurianum ferredoxin gene." Graves M.C., Mullenbach G.T., Rabinowitz J.C. Proc. Natl. Acad. Sci. U.S.A. 82:1653-1657(1985) [PubMed: 3856844] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"The amino acid sequence of Clostridium pasteurianum ferredoxin." Tanaka M., Nakashima T., Benson A.M., Mower H.F., Yasunobu K.T. Biochemistry 5:1666-1680(1966) [PubMed: 5335811] [Abstract] Cited for: PROTEIN SEQUENCE OF 2-56.
[3]	"Solution structure of the oxidized 2[4Fe-4S] ferredoxin from Clostridium pasteurianum." Bertini I., Donaire A., Feinberg B.A., Luchinat C., Piccioli M., Yuan H. Eur. J. Biochem. 232:192-205(1995) [PubMed: 7556151] [Abstract] Cited for: STRUCTURE BY NMR.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

M11214 Genomic DNA. Translation: AAA83524.1.

PIR

FECLCP. A94028.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1CLF	NMR	-	A	2-56	[»]

ProteinModelPortal

P00195.

SMR

P00195. Positions 2-56.

ModBase

Search...

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Family and domain databases

InterPro

IPR001450. 4Fe4S-bd_dom.
IPR017896. 4Fe4S_Fe-S-bd.
IPR017900. 4Fe4S_Fe_S_CS.
IPR000813. 7Fe_ferredoxin.
[Graphical view]

Pfam

PF00037. Fer4. 2 hits.
[Graphical view]

PRINTS

PR00354. 7FE8SFRDOXIN.

PROSITE

PS00198. 4FE4S_FER_1. 2 hits.
PS51379. 4FE4S_FER_2. 2 hits.
[Graphical view]

ProtoNet

Search...

Entry information

Entry name

FER_CLOPA

Accession

Primary (citable) accession number: P00195

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 21, 1986
Last sequence update:	January 23, 2007
Last modified:	September 21, 2011
This is version 82 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program