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P00191 (CP21A_BOVIN) Reviewed, UniProtKB/Swiss-Prot

Last modified January 22, 2014. Version 114. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Steroid 21-hydroxylase

EC=1.14.99.10
Alternative name(s):
21-OHase
Cytochrome P-450c21
Cytochrome P450 21
Cytochrome P450 XXI
Cytochrome P450-C21
Gene names
Name:CYP21
Synonyms:CYP21A1, CYP21A2
OrganismBos taurus (Bovine) [Reference proteome]
Taxonomic identifier9913 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos

Protein attributes

Sequence length496 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Specifically catalyzes the 21-hydroxylation of steroids. Required for the adrenal synthesis of mineralocorticoids and glucocorticoids. Ref.5

Catalytic activity

A C21 steroid + (reduced NADPH--hemoprotein reductase) + O2 = a 21-hydroxy-C(21)-steroid + (oxidized NADPH--hemoprotein reductase) + H2O. Ref.5

Cofactor

Heme group. Ref.5

Subcellular location

Endoplasmic reticulum membrane; Peripheral membrane protein. Microsome membrane; Peripheral membrane protein.

Domain

The leucine-rich hydrophobic amino acid N-terminal region probably helps to anchor the protein to the microsomal membrane.

Sequence similarities

Belongs to the cytochrome P450 family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 496496Steroid 21-hydroxylase
PRO_0000051974

Regions

Region341 – 35717Steroid-binding By similarity

Sites

Metal binding4271Iron (heme axial ligand)

Experimental info

Sequence conflict131L → K AA sequence Ref.4
Sequence conflict141A → S in AAA30487. Ref.2
Sequence conflict4011H → Y in AAA30487. Ref.2
Sequence conflict4311S → C in AAA30486. Ref.3

Secondary structure

................................................................. 496
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P00191 [UniParc].

Last modified November 1, 1990. Version 2.
Checksum: 2E87C829A7E66B31

FASTA49656,077
        10         20         30         40         50         60 
MVLAGLLLLL TLLAGAHLLW GRWKLRNLHL PPLVPGFLHL LQPNLPIHLL SLTQKLGPVY 

        70         80         90        100        110        120 
RLRLGLQEVV VLNSKRTIEE AMIRKWVDFA GRPQIPSYKL VSQRCQDISL GDYSLLWKAH 

       130        140        150        160        170        180 
KKLTRSALLL GTRSSMEPWV DQLTQEFCER MRVQAGAPVT IQKEFSLLTC SIICYLTFGN 

       190        200        210        220        230        240 
KEDTLVHAFH DCVQDLMKTW DHWSIQILDM VPFLRFFPNP GLWRLKQAIE NRDHMVEKQL 

       250        260        270        280        290        300 
TRHKESMVAG QWRDMTDYML QGVGRQRVEE GPGQLLEGHV HMSVVDLFIG GTETTASTLS 

       310        320        330        340        350        360 
WAVAFLLHHP EIQRRLQEEL DRELGPGASC SRVTYKDRAR LPLLNATIAE VLRLRPVVPL 

       370        380        390        400        410        420 
ALPHRTTRPS SIFGYDIPEG MVVIPNLQGA HLDETVWEQP HEFRPDRFLE PGANPSALAF 

       430        440        450        460        470        480 
GCGARVCLGE SLARLELFVV LLRLLQAFTL LPPPVGALPS LQPDPYCGVN LKVQPFQVRL 

       490 
QPRGVEAGAW ESASAQ 

« Hide

References

[1]"Structure of a bovine gene for P-450c21 (steroid 21-hydroxylase) defines a novel cytochrome P-450 gene family."
Chung B., Matteson K.J., Miller W.L.
Proc. Natl. Acad. Sci. U.S.A. 83:4243-4247(1986) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"Structural analysis of cloned cDNA for mRNA of microsomal cytochrome P-450(C21) which catalyzes steroid 21-hydroxylation in bovine adrenal cortex."
Yoshioka H., Morohashi K., Sogawa K., Yamane M., Kominami S., Takemori S., Okada Y., Omura T., Fujii-Kuriyama Y.
J. Biol. Chem. 261:4106-4109(1986) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[3]"Bovine steroid 21-hydroxylase: regulation of biosynthesis."
John M.E., Okamura T., Dee A., Adler B., John M.C., White P.C., Simpson E.R., Waterman M.R.
Biochemistry 25:2846-2853(1986) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 121-496.
[4]"Partial amino acid sequences of two mitochondrial and two microsomal cytochrome P-450's from adrenal cortex."
Ogishima T., Okada Y., Kominami S., Takemori S., Omura T.
J. Biochem. 94:1711-1714(1983) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 1-15.
[5]"Three-dimensional structure of steroid 21-hydroxylase (cytochrome P450 21A2) with two substrates reveals locations of disease-associated variants."
Zhao B., Lei L., Kagawa N., Sundaramoorthy M., Banerjee S., Nagy L.D., Guengerich F.P., Waterman M.R.
J. Biol. Chem. 287:10613-10622(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (3.0 ANGSTROMS) IN COMPLEX WITH HEME AND 17-HYDROXYPROGESTERONE, FUNCTION, COFACTOR, CATALYTIC ACTIVITY.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M11267 Genomic DNA. Translation: AAA83247.1.
M12918 mRNA. Translation: AAA30487.1.
K01333 mRNA. Translation: AAA30486.1.
PIRO4BOC2. A27555.
RefSeqNP_001013614.1. NM_001013596.1.
NP_777064.1. NM_174639.1.
UniGeneBt.1964.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
3QZ1X-ray3.00A/B/C/D1-496[»]
ProteinModelPortalP00191.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING9913.ENSBTAP00000007353.

Proteomic databases

PRIDEP00191.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID281741.
282425.
KEGGbta:281741.
bta:282425.

Organism-specific databases

CTD1589.
281741.

Phylogenomic databases

eggNOGCOG2124.
HOGENOMHOG000036991.
HOVERGENHBG106944.
InParanoidP00191.
KOK00513.

Enzyme and pathway databases

BRENDA1.14.99.10. 908.

Family and domain databases

Gene3D1.10.630.10. 1 hit.
InterProIPR001128. Cyt_P450.
IPR017972. Cyt_P450_CS.
IPR002401. Cyt_P450_E_grp-I.
[Graphical view]
PfamPF00067. p450. 1 hit.
[Graphical view]
PRINTSPR00463. EP450I.
PR00385. P450.
SUPFAMSSF48264. SSF48264. 1 hit.
PROSITEPS00086. CYTOCHROME_P450. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio20805661.

Entry information

Entry nameCP21A_BOVIN
AccessionPrimary (citable) accession number: P00191
Entry history
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: November 1, 1990
Last modified: January 22, 2014
This is version 114 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references