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P00191

- CP21A_BOVIN

UniProt

P00191 - CP21A_BOVIN

Protein

Steroid 21-hydroxylase

Gene

CYP21

Organism
Bos taurus (Bovine)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 115 (01 Oct 2014)
      Sequence version 2 (01 Nov 1990)
      Previous versions | rss
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    Functioni

    Specifically catalyzes the 21-hydroxylation of steroids. Required for the adrenal synthesis of mineralocorticoids and glucocorticoids.1 Publication

    Catalytic activityi

    A C(21) steroid + (reduced NADPH--hemoprotein reductase) + O2 = a 21-hydroxy-C(21)-steroid + (oxidized NADPH--hemoprotein reductase) + H2O.1 Publication

    Cofactori

    Heme group.1 Publication

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi427 – 4271Iron (heme axial ligand)

    GO - Molecular functioni

    1. heme binding Source: InterPro
    2. iron ion binding Source: InterPro
    3. steroid 21-monooxygenase activity Source: UniProtKB-EC
    4. steroid binding Source: UniProtKB-KW

    GO - Biological processi

    1. steroid biosynthetic process Source: UniProtKB-KW

    Keywords - Molecular functioni

    Monooxygenase, Oxidoreductase

    Keywords - Biological processi

    Steroidogenesis

    Keywords - Ligandi

    Heme, Iron, Lipid-binding, Metal-binding, Steroid-binding

    Enzyme and pathway databases

    BRENDAi1.14.99.10. 908.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Steroid 21-hydroxylase (EC:1.14.99.10)
    Alternative name(s):
    21-OHase
    Cytochrome P-450c21
    Cytochrome P450 21
    Cytochrome P450 XXI
    Cytochrome P450-C21
    Gene namesi
    Name:CYP21
    Synonyms:CYP21A1, CYP21A2
    OrganismiBos taurus (Bovine)
    Taxonomic identifieri9913 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
    ProteomesiUP000009136: Unplaced

    Subcellular locationi

    GO - Cellular componenti

    1. endoplasmic reticulum membrane Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Endoplasmic reticulum, Membrane, Microsome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 496496Steroid 21-hydroxylasePRO_0000051974Add
    BLAST

    Proteomic databases

    PRIDEiP00191.

    Interactioni

    Protein-protein interaction databases

    STRINGi9913.ENSBTAP00000007353.

    Structurei

    Secondary structure

    1
    496
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi41 – 433
    Helixi45 – 517
    Helixi52 – 543
    Beta strandi58 – 625
    Beta strandi64 – 674
    Beta strandi69 – 724
    Helixi77 – 804
    Turni81 – 844
    Turni96 – 1005
    Beta strandi108 – 1114
    Helixi115 – 12915
    Helixi137 – 15216
    Helixi160 – 1656
    Helixi167 – 1726
    Turni173 – 1764
    Helixi183 – 19412
    Turni195 – 2006
    Helixi203 – 2108
    Helixi212 – 2143
    Helixi220 – 24526
    Beta strandi253 – 2553
    Helixi256 – 2594
    Helixi278 – 30831
    Helixi310 – 32415
    Beta strandi326 – 3316
    Helixi337 – 3393
    Helixi342 – 35413
    Beta strandi357 – 3604
    Beta strandi373 – 3775
    Beta strandi382 – 3854
    Helixi387 – 3904
    Turni394 – 3963
    Beta strandi397 – 3993
    Beta strandi423 – 4264
    Helixi430 – 44718

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    3QZ1X-ray3.00A/B/C/D1-496[»]
    ProteinModelPortaliP00191.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni341 – 35717Steroid-bindingBy similarityAdd
    BLAST

    Domaini

    The leucine-rich hydrophobic amino acid N-terminal region probably helps to anchor the protein to the microsomal membrane.

    Sequence similaritiesi

    Belongs to the cytochrome P450 family.Curated

    Phylogenomic databases

    eggNOGiCOG2124.
    HOGENOMiHOG000036991.
    HOVERGENiHBG106944.
    InParanoidiP00191.
    KOiK00513.

    Family and domain databases

    Gene3Di1.10.630.10. 1 hit.
    InterProiIPR001128. Cyt_P450.
    IPR017972. Cyt_P450_CS.
    IPR002401. Cyt_P450_E_grp-I.
    [Graphical view]
    PfamiPF00067. p450. 1 hit.
    [Graphical view]
    PRINTSiPR00463. EP450I.
    PR00385. P450.
    SUPFAMiSSF48264. SSF48264. 1 hit.
    PROSITEiPS00086. CYTOCHROME_P450. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P00191-1 [UniParc]FASTAAdd to Basket

    « Hide

    MVLAGLLLLL TLLAGAHLLW GRWKLRNLHL PPLVPGFLHL LQPNLPIHLL    50
    SLTQKLGPVY RLRLGLQEVV VLNSKRTIEE AMIRKWVDFA GRPQIPSYKL 100
    VSQRCQDISL GDYSLLWKAH KKLTRSALLL GTRSSMEPWV DQLTQEFCER 150
    MRVQAGAPVT IQKEFSLLTC SIICYLTFGN KEDTLVHAFH DCVQDLMKTW 200
    DHWSIQILDM VPFLRFFPNP GLWRLKQAIE NRDHMVEKQL TRHKESMVAG 250
    QWRDMTDYML QGVGRQRVEE GPGQLLEGHV HMSVVDLFIG GTETTASTLS 300
    WAVAFLLHHP EIQRRLQEEL DRELGPGASC SRVTYKDRAR LPLLNATIAE 350
    VLRLRPVVPL ALPHRTTRPS SIFGYDIPEG MVVIPNLQGA HLDETVWEQP 400
    HEFRPDRFLE PGANPSALAF GCGARVCLGE SLARLELFVV LLRLLQAFTL 450
    LPPPVGALPS LQPDPYCGVN LKVQPFQVRL QPRGVEAGAW ESASAQ 496
    Length:496
    Mass (Da):56,077
    Last modified:November 1, 1990 - v2
    Checksum:i2E87C829A7E66B31
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti13 – 131L → K AA sequence (PubMed:6654880)Curated
    Sequence conflicti14 – 141A → S in AAA30487. (PubMed:3005319)Curated
    Sequence conflicti401 – 4011H → Y in AAA30487. (PubMed:3005319)Curated
    Sequence conflicti431 – 4311S → C in AAA30486. (PubMed:2424492)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M11267 Genomic DNA. Translation: AAA83247.1.
    M12918 mRNA. Translation: AAA30487.1.
    K01333 mRNA. Translation: AAA30486.1.
    PIRiA27555. O4BOC2.
    RefSeqiNP_001013614.1. NM_001013596.1.
    NP_777064.1. NM_174639.1.
    UniGeneiBt.1964.

    Genome annotation databases

    GeneIDi281741.
    282425.
    KEGGibta:281741.
    bta:282425.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M11267 Genomic DNA. Translation: AAA83247.1 .
    M12918 mRNA. Translation: AAA30487.1 .
    K01333 mRNA. Translation: AAA30486.1 .
    PIRi A27555. O4BOC2.
    RefSeqi NP_001013614.1. NM_001013596.1.
    NP_777064.1. NM_174639.1.
    UniGenei Bt.1964.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    3QZ1 X-ray 3.00 A/B/C/D 1-496 [» ]
    ProteinModelPortali P00191.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 9913.ENSBTAP00000007353.

    Proteomic databases

    PRIDEi P00191.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 281741.
    282425.
    KEGGi bta:281741.
    bta:282425.

    Organism-specific databases

    CTDi 1589.
    281741.

    Phylogenomic databases

    eggNOGi COG2124.
    HOGENOMi HOG000036991.
    HOVERGENi HBG106944.
    InParanoidi P00191.
    KOi K00513.

    Enzyme and pathway databases

    BRENDAi 1.14.99.10. 908.

    Miscellaneous databases

    NextBioi 20805661.

    Family and domain databases

    Gene3Di 1.10.630.10. 1 hit.
    InterProi IPR001128. Cyt_P450.
    IPR017972. Cyt_P450_CS.
    IPR002401. Cyt_P450_E_grp-I.
    [Graphical view ]
    Pfami PF00067. p450. 1 hit.
    [Graphical view ]
    PRINTSi PR00463. EP450I.
    PR00385. P450.
    SUPFAMi SSF48264. SSF48264. 1 hit.
    PROSITEi PS00086. CYTOCHROME_P450. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Structure of a bovine gene for P-450c21 (steroid 21-hydroxylase) defines a novel cytochrome P-450 gene family."
      Chung B., Matteson K.J., Miller W.L.
      Proc. Natl. Acad. Sci. U.S.A. 83:4243-4247(1986) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. "Structural analysis of cloned cDNA for mRNA of microsomal cytochrome P-450(C21) which catalyzes steroid 21-hydroxylation in bovine adrenal cortex."
      Yoshioka H., Morohashi K., Sogawa K., Yamane M., Kominami S., Takemori S., Okada Y., Omura T., Fujii-Kuriyama Y.
      J. Biol. Chem. 261:4106-4109(1986) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    3. Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 121-496.
    4. "Partial amino acid sequences of two mitochondrial and two microsomal cytochrome P-450's from adrenal cortex."
      Ogishima T., Okada Y., Kominami S., Takemori S., Omura T.
      J. Biochem. 94:1711-1714(1983) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 1-15.
    5. "Three-dimensional structure of steroid 21-hydroxylase (cytochrome P450 21A2) with two substrates reveals locations of disease-associated variants."
      Zhao B., Lei L., Kagawa N., Sundaramoorthy M., Banerjee S., Nagy L.D., Guengerich F.P., Waterman M.R.
      J. Biol. Chem. 287:10613-10622(2012) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (3.0 ANGSTROMS) IN COMPLEX WITH HEME AND 17-HYDROXYPROGESTERONE, FUNCTION, COFACTOR, CATALYTIC ACTIVITY.

    Entry informationi

    Entry nameiCP21A_BOVIN
    AccessioniPrimary (citable) accession number: P00191
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 21, 1986
    Last sequence update: November 1, 1990
    Last modified: October 1, 2014
    This is version 115 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3