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Protein

Steroid 21-hydroxylase

Gene

CYP21

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Specifically catalyzes the 21-hydroxylation of steroids. Required for the adrenal synthesis of mineralocorticoids and glucocorticoids.1 Publication

Catalytic activityi

A C(21) steroid + [reduced NADPH--hemoprotein reductase] + O2 = a 21-hydroxy-C(21)-steroid + [oxidized NADPH--hemoprotein reductase] + H2O.1 Publication

Cofactori

heme1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei232Substrate1 Publication1
Metal bindingi427Iron (heme axial ligand)1 Publication1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Monooxygenase, Oxidoreductase

Keywords - Biological processi

Steroidogenesis

Keywords - Ligandi

Heme, Iron, Lipid-binding, Metal-binding, Steroid-binding

Enzyme and pathway databases

BRENDAi1.14.99.10. 908.

Chemistry databases

SwissLipidsiSLP:000001620.

Names & Taxonomyi

Protein namesi
Recommended name:
Steroid 21-hydroxylase (EC:1.14.14.161 Publication)
Alternative name(s):
21-OHase
Cytochrome P-450c21
Cytochrome P450 21
Cytochrome P450 XXI
Cytochrome P450-C21
Gene namesi
Name:CYP21
Synonyms:CYP21A1, CYP21A2
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
Proteomesi
  • UP000009136 Componenti: Unplaced

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane, Microsome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000519741 – 496Steroid 21-hydroxylaseAdd BLAST496

Proteomic databases

PaxDbiP00191.
PRIDEiP00191.

Interactioni

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000054564.

Structurei

Secondary structure

1496
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi41 – 43Combined sources3
Helixi45 – 51Combined sources7
Helixi52 – 54Combined sources3
Beta strandi58 – 62Combined sources5
Beta strandi64 – 67Combined sources4
Beta strandi69 – 72Combined sources4
Helixi77 – 80Combined sources4
Turni81 – 84Combined sources4
Turni96 – 100Combined sources5
Beta strandi108 – 111Combined sources4
Helixi115 – 129Combined sources15
Helixi137 – 152Combined sources16
Helixi160 – 165Combined sources6
Helixi167 – 172Combined sources6
Turni173 – 176Combined sources4
Helixi183 – 194Combined sources12
Turni195 – 200Combined sources6
Helixi203 – 210Combined sources8
Helixi212 – 214Combined sources3
Helixi220 – 245Combined sources26
Beta strandi253 – 255Combined sources3
Helixi256 – 259Combined sources4
Helixi278 – 308Combined sources31
Helixi310 – 324Combined sources15
Beta strandi326 – 331Combined sources6
Helixi337 – 339Combined sources3
Helixi342 – 354Combined sources13
Beta strandi357 – 360Combined sources4
Beta strandi373 – 377Combined sources5
Beta strandi382 – 385Combined sources4
Helixi387 – 390Combined sources4
Turni394 – 396Combined sources3
Beta strandi397 – 399Combined sources3
Beta strandi410 – 412Combined sources3
Beta strandi423 – 426Combined sources4
Helixi430 – 447Combined sources18

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3QZ1X-ray3.00A/B/C/D1-496[»]
ProteinModelPortaliP00191.
SMRiP00191.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni341 – 357Steroid-bindingBy similarityAdd BLAST17

Domaini

The leucine-rich hydrophobic amino acid N-terminal region probably helps to anchor the protein to the microsomal membrane.

Sequence similaritiesi

Belongs to the cytochrome P450 family.Curated

Phylogenomic databases

eggNOGiKOG0156. Eukaryota.
COG2124. LUCA.
HOGENOMiHOG000036991.
HOVERGENiHBG106944.
InParanoidiP00191.
KOiK00513.

Family and domain databases

Gene3Di1.10.630.10. 1 hit.
InterProiIPR001128. Cyt_P450.
IPR017972. Cyt_P450_CS.
IPR002401. Cyt_P450_E_grp-I.
[Graphical view]
PfamiPF00067. p450. 1 hit.
[Graphical view]
PRINTSiPR00463. EP450I.
PR00385. P450.
SUPFAMiSSF48264. SSF48264. 1 hit.
PROSITEiPS00086. CYTOCHROME_P450. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P00191-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVLAGLLLLL TLLAGAHLLW GRWKLRNLHL PPLVPGFLHL LQPNLPIHLL
60 70 80 90 100
SLTQKLGPVY RLRLGLQEVV VLNSKRTIEE AMIRKWVDFA GRPQIPSYKL
110 120 130 140 150
VSQRCQDISL GDYSLLWKAH KKLTRSALLL GTRSSMEPWV DQLTQEFCER
160 170 180 190 200
MRVQAGAPVT IQKEFSLLTC SIICYLTFGN KEDTLVHAFH DCVQDLMKTW
210 220 230 240 250
DHWSIQILDM VPFLRFFPNP GLWRLKQAIE NRDHMVEKQL TRHKESMVAG
260 270 280 290 300
QWRDMTDYML QGVGRQRVEE GPGQLLEGHV HMSVVDLFIG GTETTASTLS
310 320 330 340 350
WAVAFLLHHP EIQRRLQEEL DRELGPGASC SRVTYKDRAR LPLLNATIAE
360 370 380 390 400
VLRLRPVVPL ALPHRTTRPS SIFGYDIPEG MVVIPNLQGA HLDETVWEQP
410 420 430 440 450
HEFRPDRFLE PGANPSALAF GCGARVCLGE SLARLELFVV LLRLLQAFTL
460 470 480 490
LPPPVGALPS LQPDPYCGVN LKVQPFQVRL QPRGVEAGAW ESASAQ
Length:496
Mass (Da):56,077
Last modified:November 1, 1990 - v2
Checksum:i2E87C829A7E66B31
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti13L → K AA sequence (PubMed:6654880).Curated1
Sequence conflicti14A → S in AAA30487 (PubMed:3005319).Curated1
Sequence conflicti401H → Y in AAA30487 (PubMed:3005319).Curated1
Sequence conflicti431S → C in AAA30486 (PubMed:2424492).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M11267 Genomic DNA. Translation: AAA83247.1.
M12918 mRNA. Translation: AAA30487.1.
K01333 mRNA. Translation: AAA30486.1.
PIRiA27555. O4BOC2.
RefSeqiNP_001013614.1. NM_001013596.1.
NP_777064.1. NM_174639.1.
UniGeneiBt.1964.

Genome annotation databases

GeneIDi281741.
282425.
KEGGibta:281741.
bta:282425.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M11267 Genomic DNA. Translation: AAA83247.1.
M12918 mRNA. Translation: AAA30487.1.
K01333 mRNA. Translation: AAA30486.1.
PIRiA27555. O4BOC2.
RefSeqiNP_001013614.1. NM_001013596.1.
NP_777064.1. NM_174639.1.
UniGeneiBt.1964.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3QZ1X-ray3.00A/B/C/D1-496[»]
ProteinModelPortaliP00191.
SMRiP00191.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000054564.

Chemistry databases

SwissLipidsiSLP:000001620.

Proteomic databases

PaxDbiP00191.
PRIDEiP00191.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi281741.
282425.
KEGGibta:281741.
bta:282425.

Organism-specific databases

CTDi1589.
281741.

Phylogenomic databases

eggNOGiKOG0156. Eukaryota.
COG2124. LUCA.
HOGENOMiHOG000036991.
HOVERGENiHBG106944.
InParanoidiP00191.
KOiK00513.

Enzyme and pathway databases

BRENDAi1.14.99.10. 908.

Family and domain databases

Gene3Di1.10.630.10. 1 hit.
InterProiIPR001128. Cyt_P450.
IPR017972. Cyt_P450_CS.
IPR002401. Cyt_P450_E_grp-I.
[Graphical view]
PfamiPF00067. p450. 1 hit.
[Graphical view]
PRINTSiPR00463. EP450I.
PR00385. P450.
SUPFAMiSSF48264. SSF48264. 1 hit.
PROSITEiPS00086. CYTOCHROME_P450. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCP21A_BOVIN
AccessioniPrimary (citable) accession number: P00191
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: November 1, 1990
Last modified: November 2, 2016
This is version 127 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.