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Protein

Steroid 21-hydroxylase

Gene

CYP21

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Specifically catalyzes the 21-hydroxylation of steroids. Required for the adrenal synthesis of mineralocorticoids and glucocorticoids.1 Publication

Catalytic activityi

A C(21) steroid + (reduced NADPH--hemoprotein reductase) + O2 = a 21-hydroxy-C(21)-steroid + (oxidized NADPH--hemoprotein reductase) + H2O.1 Publication

Cofactori

heme1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi427 – 4271Iron (heme axial ligand)

GO - Molecular functioni

  1. heme binding Source: InterPro
  2. iron ion binding Source: InterPro
  3. steroid 21-monooxygenase activity Source: UniProtKB-EC
  4. steroid binding Source: UniProtKB-KW

GO - Biological processi

  1. steroid biosynthetic process Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Monooxygenase, Oxidoreductase

Keywords - Biological processi

Steroidogenesis

Keywords - Ligandi

Heme, Iron, Lipid-binding, Metal-binding, Steroid-binding

Enzyme and pathway databases

BRENDAi1.14.99.10. 908.

Names & Taxonomyi

Protein namesi
Recommended name:
Steroid 21-hydroxylase (EC:1.14.99.10)
Alternative name(s):
21-OHase
Cytochrome P-450c21
Cytochrome P450 21
Cytochrome P450 XXI
Cytochrome P450-C21
Gene namesi
Name:CYP21
Synonyms:CYP21A1, CYP21A2
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
ProteomesiUP000009136: Unplaced

Subcellular locationi

GO - Cellular componenti

  1. endoplasmic reticulum membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane, Microsome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 496496Steroid 21-hydroxylasePRO_0000051974Add
BLAST

Proteomic databases

PRIDEiP00191.

Interactioni

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000007353.

Structurei

Secondary structure

1
496
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi41 – 433Combined sources
Helixi45 – 517Combined sources
Helixi52 – 543Combined sources
Beta strandi58 – 625Combined sources
Beta strandi64 – 674Combined sources
Beta strandi69 – 724Combined sources
Helixi77 – 804Combined sources
Turni81 – 844Combined sources
Turni96 – 1005Combined sources
Beta strandi108 – 1114Combined sources
Helixi115 – 12915Combined sources
Helixi137 – 15216Combined sources
Helixi160 – 1656Combined sources
Helixi167 – 1726Combined sources
Turni173 – 1764Combined sources
Helixi183 – 19412Combined sources
Turni195 – 2006Combined sources
Helixi203 – 2108Combined sources
Helixi212 – 2143Combined sources
Helixi220 – 24526Combined sources
Beta strandi253 – 2553Combined sources
Helixi256 – 2594Combined sources
Helixi278 – 30831Combined sources
Helixi310 – 32415Combined sources
Beta strandi326 – 3316Combined sources
Helixi337 – 3393Combined sources
Helixi342 – 35413Combined sources
Beta strandi357 – 3604Combined sources
Beta strandi373 – 3775Combined sources
Beta strandi382 – 3854Combined sources
Helixi387 – 3904Combined sources
Turni394 – 3963Combined sources
Beta strandi397 – 3993Combined sources
Beta strandi423 – 4264Combined sources
Helixi430 – 44718Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3QZ1X-ray3.00A/B/C/D1-496[»]
ProteinModelPortaliP00191.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni341 – 35717Steroid-bindingBy similarityAdd
BLAST

Domaini

The leucine-rich hydrophobic amino acid N-terminal region probably helps to anchor the protein to the microsomal membrane.

Sequence similaritiesi

Belongs to the cytochrome P450 family.Curated

Phylogenomic databases

eggNOGiCOG2124.
HOGENOMiHOG000036991.
HOVERGENiHBG106944.
InParanoidiP00191.
KOiK00513.

Family and domain databases

Gene3Di1.10.630.10. 1 hit.
InterProiIPR001128. Cyt_P450.
IPR017972. Cyt_P450_CS.
IPR002401. Cyt_P450_E_grp-I.
[Graphical view]
PfamiPF00067. p450. 1 hit.
[Graphical view]
PRINTSiPR00463. EP450I.
PR00385. P450.
SUPFAMiSSF48264. SSF48264. 1 hit.
PROSITEiPS00086. CYTOCHROME_P450. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P00191-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MVLAGLLLLL TLLAGAHLLW GRWKLRNLHL PPLVPGFLHL LQPNLPIHLL
60 70 80 90 100
SLTQKLGPVY RLRLGLQEVV VLNSKRTIEE AMIRKWVDFA GRPQIPSYKL
110 120 130 140 150
VSQRCQDISL GDYSLLWKAH KKLTRSALLL GTRSSMEPWV DQLTQEFCER
160 170 180 190 200
MRVQAGAPVT IQKEFSLLTC SIICYLTFGN KEDTLVHAFH DCVQDLMKTW
210 220 230 240 250
DHWSIQILDM VPFLRFFPNP GLWRLKQAIE NRDHMVEKQL TRHKESMVAG
260 270 280 290 300
QWRDMTDYML QGVGRQRVEE GPGQLLEGHV HMSVVDLFIG GTETTASTLS
310 320 330 340 350
WAVAFLLHHP EIQRRLQEEL DRELGPGASC SRVTYKDRAR LPLLNATIAE
360 370 380 390 400
VLRLRPVVPL ALPHRTTRPS SIFGYDIPEG MVVIPNLQGA HLDETVWEQP
410 420 430 440 450
HEFRPDRFLE PGANPSALAF GCGARVCLGE SLARLELFVV LLRLLQAFTL
460 470 480 490
LPPPVGALPS LQPDPYCGVN LKVQPFQVRL QPRGVEAGAW ESASAQ
Length:496
Mass (Da):56,077
Last modified:November 1, 1990 - v2
Checksum:i2E87C829A7E66B31
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti13 – 131L → K AA sequence (PubMed:6654880)Curated
Sequence conflicti14 – 141A → S in AAA30487. (PubMed:3005319)Curated
Sequence conflicti401 – 4011H → Y in AAA30487. (PubMed:3005319)Curated
Sequence conflicti431 – 4311S → C in AAA30486. (PubMed:2424492)Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M11267 Genomic DNA. Translation: AAA83247.1.
M12918 mRNA. Translation: AAA30487.1.
K01333 mRNA. Translation: AAA30486.1.
PIRiA27555. O4BOC2.
RefSeqiNP_001013614.1. NM_001013596.1.
NP_777064.1. NM_174639.1.
UniGeneiBt.1964.

Genome annotation databases

GeneIDi281741.
282425.
KEGGibta:281741.
bta:282425.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M11267 Genomic DNA. Translation: AAA83247.1.
M12918 mRNA. Translation: AAA30487.1.
K01333 mRNA. Translation: AAA30486.1.
PIRiA27555. O4BOC2.
RefSeqiNP_001013614.1. NM_001013596.1.
NP_777064.1. NM_174639.1.
UniGeneiBt.1964.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3QZ1X-ray3.00A/B/C/D1-496[»]
ProteinModelPortaliP00191.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000007353.

Proteomic databases

PRIDEiP00191.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi281741.
282425.
KEGGibta:281741.
bta:282425.

Organism-specific databases

CTDi1589.
281741.

Phylogenomic databases

eggNOGiCOG2124.
HOGENOMiHOG000036991.
HOVERGENiHBG106944.
InParanoidiP00191.
KOiK00513.

Enzyme and pathway databases

BRENDAi1.14.99.10. 908.

Miscellaneous databases

NextBioi20805661.

Family and domain databases

Gene3Di1.10.630.10. 1 hit.
InterProiIPR001128. Cyt_P450.
IPR017972. Cyt_P450_CS.
IPR002401. Cyt_P450_E_grp-I.
[Graphical view]
PfamiPF00067. p450. 1 hit.
[Graphical view]
PRINTSiPR00463. EP450I.
PR00385. P450.
SUPFAMiSSF48264. SSF48264. 1 hit.
PROSITEiPS00086. CYTOCHROME_P450. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Structure of a bovine gene for P-450c21 (steroid 21-hydroxylase) defines a novel cytochrome P-450 gene family."
    Chung B., Matteson K.J., Miller W.L.
    Proc. Natl. Acad. Sci. U.S.A. 83:4243-4247(1986) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "Structural analysis of cloned cDNA for mRNA of microsomal cytochrome P-450(C21) which catalyzes steroid 21-hydroxylation in bovine adrenal cortex."
    Yoshioka H., Morohashi K., Sogawa K., Yamane M., Kominami S., Takemori S., Okada Y., Omura T., Fujii-Kuriyama Y.
    J. Biol. Chem. 261:4106-4109(1986) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  3. Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 121-496.
  4. "Partial amino acid sequences of two mitochondrial and two microsomal cytochrome P-450's from adrenal cortex."
    Ogishima T., Okada Y., Kominami S., Takemori S., Omura T.
    J. Biochem. 94:1711-1714(1983) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 1-15.
  5. "Three-dimensional structure of steroid 21-hydroxylase (cytochrome P450 21A2) with two substrates reveals locations of disease-associated variants."
    Zhao B., Lei L., Kagawa N., Sundaramoorthy M., Banerjee S., Nagy L.D., Guengerich F.P., Waterman M.R.
    J. Biol. Chem. 287:10613-10622(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (3.0 ANGSTROMS) IN COMPLEX WITH HEME AND 17-HYDROXYPROGESTERONE, FUNCTION, COFACTOR, CATALYTIC ACTIVITY.

Entry informationi

Entry nameiCP21A_BOVIN
AccessioniPrimary (citable) accession number: P00191
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: November 1, 1990
Last modified: January 7, 2015
This is version 118 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.