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Protein

Cytochrome P450 1A2

Gene

CYP1A2

Organism
Oryctolagus cuniculus (Rabbit)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Cytochromes P450 are a group of heme-thiolate monooxygenases. In liver microsomes, this enzyme is involved in an NADPH-dependent electron transport pathway. It oxidizes a variety of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics. Most active in catalyzing 2-hydroxylation.

Catalytic activityi

RH + [reduced NADPH--hemoprotein reductase] + O2 = ROH + [oxidized NADPH--hemoprotein reductase] + H2O.By similarity

Cofactori

hemeBy similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei226SubstrateBy similarity1
Sitei403Involved in electron transfer with reductase1
Metal bindingi458Iron (heme axial ligand)1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Monooxygenase, Oxidoreductase

Keywords - Biological processi

Lipid metabolism, Steroid metabolism, Sterol metabolism

Keywords - Ligandi

Heme, Iron, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Cytochrome P450 1A2 (EC:1.14.14.1By similarity)
Alternative name(s):
CYPIA2
Cholesterol 25-hydroxylaseBy similarity
Cytochrome P450 isozyme 4
Short name:
Cytochrome P450 LM4
Cytochrome P450-PM4
Gene namesi
Name:CYP1A2
OrganismiOryctolagus cuniculus (Rabbit)
Taxonomic identifieri9986 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresLagomorphaLeporidaeOryctolagus
Proteomesi
  • UP000001811 Componenti: Unplaced

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane, Microsome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved1 Publication
ChainiPRO_00000516552 – 516Cytochrome P450 1A2Add BLAST515

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi69O-linked (GlcNAc)By similarity1

Keywords - PTMi

Glycoprotein

Proteomic databases

PRIDEiP00187.

Expressioni

Inductioni

By beta-naphthoflavone and 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD).

Interactioni

Protein-protein interaction databases

STRINGi9986.ENSOCUP00000008665.

Structurei

3D structure databases

ProteinModelPortaliP00187.
SMRiP00187.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni361 – 365Substrate binding5

Sequence similaritiesi

Belongs to the cytochrome P450 family.Curated

Phylogenomic databases

eggNOGiKOG0156. Eukaryota.
COG2124. LUCA.
HOGENOMiHOG000036991.
HOVERGENiHBG106944.
InParanoidiP00187.
KOiK07409.

Family and domain databases

Gene3Di1.10.630.10. 1 hit.
InterProiIPR001128. Cyt_P450.
IPR017972. Cyt_P450_CS.
IPR002401. Cyt_P450_E_grp-I.
IPR008066. Cyt_P450_E_grp-I_CYP1.
[Graphical view]
PfamiPF00067. p450. 1 hit.
[Graphical view]
PRINTSiPR00463. EP450I.
PR01683. EP450ICYP1A.
PR00385. P450.
SUPFAMiSSF48264. SSF48264. 1 hit.
PROSITEiPS00086. CYTOCHROME_P450. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P00187-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAMSPAAPLS VTELLLVSAV FCLVFWAVRA SRPKVPKGLK RLPGPWGWPL
60 70 80 90 100
LGHLLTLGKN PHVALARLSR RYGDVFQIRL GSTPVVVLSG LDTIKQALVR
110 120 130 140 150
QGDDFKGRPD LYSSSFITEG QSMTFSPDSG PVWAARRRLA QDSLKSFSIA
160 170 180 190 200
SNPASSSSCY LEEHVSQEAE NLIGRFQELM AAVGRFDPYS QLVVSAARVI
210 220 230 240 250
GAMCFGRRFP QGSEEMLDVV RNSSKFVETA SSGSPVDFFP ILRYLPNRPL
260 270 280 290 300
QRFKDFNQRF LRFLQKTVRE HYEDFDRNSI QDITGALFKH SEKNSKANSG
310 320 330 340 350
LIPQEKIVNL VNDIFGAGFD TITTALSWSL MYLVTNPRRQ RKIQEELDAV
360 370 380 390 400
VGRARQPRLS DRPQLPYLEA FILELFRHTS FVPFTIPHST TRDTTLNGFH
410 420 430 440 450
IPKECCIFIN QWQINHDPQL WGDPEEFRPE RFLTADGAAI NKPLSEKVTL
460 470 480 490 500
FGLGKRRCIG ETLARWEVFL FLAILLQRLE FSVPPGVPVD LTPIYGLTMK
510
HPRCEHVQAR PRFSDQ
Length:516
Mass (Da):58,334
Last modified:January 23, 2007 - v3
Checksum:i3FD074D74A47C3EF
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti22C → S AA sequence (PubMed:3949797).Curated1
Sequence conflicti67R → P in AAA31437 (PubMed:2847925).Curated1
Sequence conflicti70R → Q AA sequence (PubMed:3949797).Curated1
Sequence conflicti92D → N AA sequence (PubMed:3949797).Curated1
Sequence conflicti93T → H in AAA31433 (PubMed:2991917).Curated1
Sequence conflicti121Q → H in CAA29171 (PubMed:3667560).Curated1
Sequence conflicti172L → F AA sequence (PubMed:3949797).Curated1
Sequence conflicti194V → S AA sequence (PubMed:3949797).Curated1
Sequence conflicti208 – 212RFPQG → FPQGM AA sequence (PubMed:3949797).Curated5
Sequence conflicti208R → H in AAA31437 (PubMed:2847925).Curated1
Sequence conflicti208R → H in CAA32066 (PubMed:2847925).Curated1
Sequence conflicti208R → H in AAA31433 (PubMed:2991917).Curated1
Sequence conflicti247 – 251NRPLQ → QPNLR AA sequence (PubMed:3949797).Curated5
Sequence conflicti288F → I in AAA31433 (PubMed:2991917).Curated1
Sequence conflicti291 – 302SEKNS…NSGLI → NEMDSMDDGAHV in AAA31433 (PubMed:2991917).CuratedAdd BLAST12
Sequence conflicti309N → T in AAA31433 (PubMed:2991917).Curated1
Sequence conflicti354 – 355AR → PG in AAA31437 (PubMed:2847925).Curated2
Sequence conflicti354 – 355AR → PG in CAA32066 (PubMed:2847925).Curated2
Sequence conflicti358R → L in AAA31433 (PubMed:2991917).Curated1
Sequence conflicti359L → I AA sequence (PubMed:3949797).Curated1
Sequence conflicti359L → I in AAA31433 (PubMed:2991917).Curated1
Sequence conflicti462T → I in AAA31433 (PubMed:2991917).Curated1
Sequence conflicti494I → T AA sequence (PubMed:3949797).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural varianti174G → S.1 Publication1
Natural varianti233G → S.1 Publication1
Natural varianti299S → G.1 Publication1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M36538 mRNA. Translation: AAA31437.1.
X13853 mRNA. Translation: CAA32066.1.
X05686 mRNA. Translation: CAA29171.1.
M11728 mRNA. Translation: AAA31433.1.
PIRiB27821. O4RBBN.
RefSeqiNP_001164592.1. NM_001171121.1.
UniGeneiOcu.1943.

Genome annotation databases

GeneIDi100328937.
KEGGiocu:100328937.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M36538 mRNA. Translation: AAA31437.1.
X13853 mRNA. Translation: CAA32066.1.
X05686 mRNA. Translation: CAA29171.1.
M11728 mRNA. Translation: AAA31433.1.
PIRiB27821. O4RBBN.
RefSeqiNP_001164592.1. NM_001171121.1.
UniGeneiOcu.1943.

3D structure databases

ProteinModelPortaliP00187.
SMRiP00187.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9986.ENSOCUP00000008665.

Proteomic databases

PRIDEiP00187.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi100328937.
KEGGiocu:100328937.

Phylogenomic databases

eggNOGiKOG0156. Eukaryota.
COG2124. LUCA.
HOGENOMiHOG000036991.
HOVERGENiHBG106944.
InParanoidiP00187.
KOiK07409.

Family and domain databases

Gene3Di1.10.630.10. 1 hit.
InterProiIPR001128. Cyt_P450.
IPR017972. Cyt_P450_CS.
IPR002401. Cyt_P450_E_grp-I.
IPR008066. Cyt_P450_E_grp-I_CYP1.
[Graphical view]
PfamiPF00067. p450. 1 hit.
[Graphical view]
PRINTSiPR00463. EP450I.
PR01683. EP450ICYP1A.
PR00385. P450.
SUPFAMiSSF48264. SSF48264. 1 hit.
PROSITEiPS00086. CYTOCHROME_P450. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCP1A2_RABIT
AccessioniPrimary (citable) accession number: P00187
Secondary accession number(s): Q29526
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: January 23, 2007
Last modified: November 2, 2016
This is version 135 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.