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Protein

Cytochrome P450 1A2

Gene

CYP1A2

Organism
Oryctolagus cuniculus (Rabbit)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Cytochromes P450 are a group of heme-thiolate monooxygenases. In liver microsomes, this enzyme is involved in an NADPH-dependent electron transport pathway. It oxidizes a variety of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics. Most active in catalyzing 2-hydroxylation.

Catalytic activityi

RH + [reduced NADPH--hemoprotein reductase] + O2 = ROH + [oxidized NADPH--hemoprotein reductase] + H2O.By similarity

Cofactori

hemeBy similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei226SubstrateBy similarity1
Sitei403Involved in electron transfer with reductase1
Metal bindingi458Iron (heme axial ligand)1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionMonooxygenase, Oxidoreductase
Biological processLipid metabolism, Steroid metabolism, Sterol metabolism
LigandHeme, Iron, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Cytochrome P450 1A2 (EC:1.14.14.1By similarity)
Alternative name(s):
CYPIA2
Cholesterol 25-hydroxylaseBy similarity
Cytochrome P450 isozyme 4
Short name:
Cytochrome P450 LM4
Cytochrome P450-PM4
Gene namesi
Name:CYP1A2
OrganismiOryctolagus cuniculus (Rabbit)
Taxonomic identifieri9986 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresLagomorphaLeporidaeOryctolagus
Proteomesi
  • UP000001811 Componenti: Unplaced

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane, Microsome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved1 Publication
ChainiPRO_00000516552 – 516Cytochrome P450 1A2Add BLAST515

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi69O-linked (GlcNAc) serineBy similarity1

Keywords - PTMi

Glycoprotein

Expressioni

Inductioni

By beta-naphthoflavone and 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD).

Interactioni

Protein-protein interaction databases

STRINGi9986.ENSOCUP00000008665

Structurei

3D structure databases

ProteinModelPortaliP00187
SMRiP00187
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni361 – 365Substrate binding5

Sequence similaritiesi

Belongs to the cytochrome P450 family.Curated

Phylogenomic databases

eggNOGiKOG0156 Eukaryota
COG2124 LUCA
HOGENOMiHOG000036991
HOVERGENiHBG106944
InParanoidiP00187
KOiK07409

Family and domain databases

Gene3Di1.10.630.10, 1 hit
InterProiView protein in InterPro
IPR001128 Cyt_P450
IPR017972 Cyt_P450_CS
IPR002401 Cyt_P450_E_grp-I
IPR008066 Cyt_P450_E_grp-I_CYP1
IPR036396 Cyt_P450_sf
PfamiView protein in Pfam
PF00067 p450, 1 hit
PRINTSiPR00463 EP450I
PR01683 EP450ICYP1A
PR00385 P450
SUPFAMiSSF48264 SSF48264, 1 hit
PROSITEiView protein in PROSITE
PS00086 CYTOCHROME_P450, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P00187-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAMSPAAPLS VTELLLVSAV FCLVFWAVRA SRPKVPKGLK RLPGPWGWPL
60 70 80 90 100
LGHLLTLGKN PHVALARLSR RYGDVFQIRL GSTPVVVLSG LDTIKQALVR
110 120 130 140 150
QGDDFKGRPD LYSSSFITEG QSMTFSPDSG PVWAARRRLA QDSLKSFSIA
160 170 180 190 200
SNPASSSSCY LEEHVSQEAE NLIGRFQELM AAVGRFDPYS QLVVSAARVI
210 220 230 240 250
GAMCFGRRFP QGSEEMLDVV RNSSKFVETA SSGSPVDFFP ILRYLPNRPL
260 270 280 290 300
QRFKDFNQRF LRFLQKTVRE HYEDFDRNSI QDITGALFKH SEKNSKANSG
310 320 330 340 350
LIPQEKIVNL VNDIFGAGFD TITTALSWSL MYLVTNPRRQ RKIQEELDAV
360 370 380 390 400
VGRARQPRLS DRPQLPYLEA FILELFRHTS FVPFTIPHST TRDTTLNGFH
410 420 430 440 450
IPKECCIFIN QWQINHDPQL WGDPEEFRPE RFLTADGAAI NKPLSEKVTL
460 470 480 490 500
FGLGKRRCIG ETLARWEVFL FLAILLQRLE FSVPPGVPVD LTPIYGLTMK
510
HPRCEHVQAR PRFSDQ
Length:516
Mass (Da):58,334
Last modified:January 23, 2007 - v3
Checksum:i3FD074D74A47C3EF
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti22C → S AA sequence (PubMed:3949797).Curated1
Sequence conflicti67R → P in AAA31437 (PubMed:2847925).Curated1
Sequence conflicti70R → Q AA sequence (PubMed:3949797).Curated1
Sequence conflicti92D → N AA sequence (PubMed:3949797).Curated1
Sequence conflicti93T → H in AAA31433 (PubMed:2991917).Curated1
Sequence conflicti121Q → H in CAA29171 (PubMed:3667560).Curated1
Sequence conflicti172L → F AA sequence (PubMed:3949797).Curated1
Sequence conflicti194V → S AA sequence (PubMed:3949797).Curated1
Sequence conflicti208 – 212RFPQG → FPQGM AA sequence (PubMed:3949797).Curated5
Sequence conflicti208R → H in AAA31437 (PubMed:2847925).Curated1
Sequence conflicti208R → H in CAA32066 (PubMed:2847925).Curated1
Sequence conflicti208R → H in AAA31433 (PubMed:2991917).Curated1
Sequence conflicti247 – 251NRPLQ → QPNLR AA sequence (PubMed:3949797).Curated5
Sequence conflicti288F → I in AAA31433 (PubMed:2991917).Curated1
Sequence conflicti291 – 302SEKNS…NSGLI → NEMDSMDDGAHV in AAA31433 (PubMed:2991917).CuratedAdd BLAST12
Sequence conflicti309N → T in AAA31433 (PubMed:2991917).Curated1
Sequence conflicti354 – 355AR → PG in AAA31437 (PubMed:2847925).Curated2
Sequence conflicti354 – 355AR → PG in CAA32066 (PubMed:2847925).Curated2
Sequence conflicti358R → L in AAA31433 (PubMed:2991917).Curated1
Sequence conflicti359L → I AA sequence (PubMed:3949797).Curated1
Sequence conflicti359L → I in AAA31433 (PubMed:2991917).Curated1
Sequence conflicti462T → I in AAA31433 (PubMed:2991917).Curated1
Sequence conflicti494I → T AA sequence (PubMed:3949797).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural varianti174G → S1 Publication1
Natural varianti233G → S1 Publication1
Natural varianti299S → G1 Publication1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M36538 mRNA Translation: AAA31437.1
X13853 mRNA Translation: CAA32066.1
X05686 mRNA Translation: CAA29171.1
M11728 mRNA Translation: AAA31433.1
PIRiB27821 O4RBBN
RefSeqiNP_001164592.1, NM_001171121.1
UniGeneiOcu.1943

Genome annotation databases

GeneIDi100328937
KEGGiocu:100328937

Similar proteinsi

Entry informationi

Entry nameiCP1A2_RABIT
AccessioniPrimary (citable) accession number: P00187
Secondary accession number(s): Q29526
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: January 23, 2007
Last modified: May 23, 2018
This is version 140 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome
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Main funding by: National Institutes of Health