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P00187

- CP1A2_RABIT

UniProt

P00187 - CP1A2_RABIT

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Protein

Cytochrome P450 1A2

Gene

CYP1A2

Organism
Oryctolagus cuniculus (Rabbit)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Cytochromes P450 are a group of heme-thiolate monooxygenases. In liver microsomes, this enzyme is involved in an NADPH-dependent electron transport pathway. It oxidizes a variety of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics. Most active in catalyzing 2-hydroxylation.

Catalytic activityi

RH + reduced flavoprotein + O2 = ROH + oxidized flavoprotein + H2O.

Cofactori

hemeBy similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei226 – 2261SubstrateBy similarity
Sitei403 – 4031Involved in electron transfer with reductase
Metal bindingi458 – 4581Iron (heme axial ligand)

GO - Molecular functioni

  1. aromatase activity Source: UniProtKB-EC
  2. heme binding Source: UniProtKB
  3. iron ion binding Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Monooxygenase, Oxidoreductase

Keywords - Ligandi

Heme, Iron, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Cytochrome P450 1A2 (EC:1.14.14.1)
Alternative name(s):
CYPIA2
Cytochrome P450 isozyme 4
Short name:
Cytochrome P450 LM4
Cytochrome P450-PM4
Gene namesi
Name:CYP1A2
OrganismiOryctolagus cuniculus (Rabbit)
Taxonomic identifieri9986 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresLagomorphaLeporidaeOryctolagus
ProteomesiUP000001811: Unplaced

Subcellular locationi

GO - Cellular componenti

  1. endoplasmic reticulum Source: UniProtKB-KW
  2. membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane, Microsome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed1 Publication
Chaini2 – 516515Cytochrome P450 1A2PRO_0000051655Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi69 – 691O-linked (GlcNAc)By similarity

Keywords - PTMi

Glycoprotein

Expressioni

Inductioni

By beta-naphthoflavone and 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD).

Interactioni

Protein-protein interaction databases

STRINGi9986.ENSOCUP00000008665.

Structurei

3D structure databases

ProteinModelPortaliP00187.
SMRiP00187. Positions 34-514.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni361 – 3655Substrate binding

Sequence similaritiesi

Belongs to the cytochrome P450 family.Curated

Phylogenomic databases

eggNOGiCOG2124.
HOGENOMiHOG000036991.
HOVERGENiHBG106944.
InParanoidiP00187.

Family and domain databases

Gene3Di1.10.630.10. 1 hit.
InterProiIPR001128. Cyt_P450.
IPR017972. Cyt_P450_CS.
IPR002401. Cyt_P450_E_grp-I.
IPR008066. Cyt_P450_E_grp-I_CYP1.
[Graphical view]
PfamiPF00067. p450. 1 hit.
[Graphical view]
PRINTSiPR00463. EP450I.
PR01683. EP450ICYP1A.
PR00385. P450.
SUPFAMiSSF48264. SSF48264. 1 hit.
PROSITEiPS00086. CYTOCHROME_P450. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P00187-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MAMSPAAPLS VTELLLVSAV FCLVFWAVRA SRPKVPKGLK RLPGPWGWPL
60 70 80 90 100
LGHLLTLGKN PHVALARLSR RYGDVFQIRL GSTPVVVLSG LDTIKQALVR
110 120 130 140 150
QGDDFKGRPD LYSSSFITEG QSMTFSPDSG PVWAARRRLA QDSLKSFSIA
160 170 180 190 200
SNPASSSSCY LEEHVSQEAE NLIGRFQELM AAVGRFDPYS QLVVSAARVI
210 220 230 240 250
GAMCFGRRFP QGSEEMLDVV RNSSKFVETA SSGSPVDFFP ILRYLPNRPL
260 270 280 290 300
QRFKDFNQRF LRFLQKTVRE HYEDFDRNSI QDITGALFKH SEKNSKANSG
310 320 330 340 350
LIPQEKIVNL VNDIFGAGFD TITTALSWSL MYLVTNPRRQ RKIQEELDAV
360 370 380 390 400
VGRARQPRLS DRPQLPYLEA FILELFRHTS FVPFTIPHST TRDTTLNGFH
410 420 430 440 450
IPKECCIFIN QWQINHDPQL WGDPEEFRPE RFLTADGAAI NKPLSEKVTL
460 470 480 490 500
FGLGKRRCIG ETLARWEVFL FLAILLQRLE FSVPPGVPVD LTPIYGLTMK
510
HPRCEHVQAR PRFSDQ
Length:516
Mass (Da):58,334
Last modified:January 23, 2007 - v3
Checksum:i3FD074D74A47C3EF
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti22 – 221C → S AA sequence (PubMed:3949797)Curated
Sequence conflicti67 – 671R → P in AAA31437. (PubMed:2847925)Curated
Sequence conflicti70 – 701R → Q AA sequence (PubMed:3949797)Curated
Sequence conflicti92 – 921D → N AA sequence (PubMed:3949797)Curated
Sequence conflicti93 – 931T → H in AAA31433. (PubMed:2991917)Curated
Sequence conflicti121 – 1211Q → H in CAA29171. (PubMed:3667560)Curated
Sequence conflicti172 – 1721L → F AA sequence (PubMed:3949797)Curated
Sequence conflicti194 – 1941V → S AA sequence (PubMed:3949797)Curated
Sequence conflicti208 – 2125RFPQG → FPQGM AA sequence (PubMed:3949797)Curated
Sequence conflicti208 – 2081R → H in AAA31437. (PubMed:2847925)Curated
Sequence conflicti208 – 2081R → H in CAA32066. (PubMed:2847925)Curated
Sequence conflicti208 – 2081R → H in AAA31433. (PubMed:2991917)Curated
Sequence conflicti247 – 2515NRPLQ → QPNLR AA sequence (PubMed:3949797)Curated
Sequence conflicti288 – 2881F → I in AAA31433. (PubMed:2991917)Curated
Sequence conflicti291 – 30212SEKNS…NSGLI → NEMDSMDDGAHV in AAA31433. (PubMed:2991917)CuratedAdd
BLAST
Sequence conflicti309 – 3091N → T in AAA31433. (PubMed:2991917)Curated
Sequence conflicti354 – 3552AR → PG in AAA31437. (PubMed:2847925)Curated
Sequence conflicti354 – 3552AR → PG in CAA32066. (PubMed:2847925)Curated
Sequence conflicti358 – 3581R → L in AAA31433. (PubMed:2991917)Curated
Sequence conflicti359 – 3591L → I AA sequence (PubMed:3949797)Curated
Sequence conflicti359 – 3591L → I in AAA31433. (PubMed:2991917)Curated
Sequence conflicti462 – 4621T → I in AAA31433. (PubMed:2991917)Curated
Sequence conflicti494 – 4941I → T AA sequence (PubMed:3949797)Curated

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti174 – 1741G → S.1 Publication
Natural varianti233 – 2331G → S.1 Publication
Natural varianti299 – 2991S → G.1 Publication

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M36538 mRNA. Translation: AAA31437.1.
X13853 mRNA. Translation: CAA32066.1.
X05686 mRNA. Translation: CAA29171.1.
M11728 mRNA. Translation: AAA31433.1.
PIRiB27821. O4RBBN.
RefSeqiNP_001164592.1. NM_001171121.1.
UniGeneiOcu.1943.

Genome annotation databases

GeneIDi100328937.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M36538 mRNA. Translation: AAA31437.1 .
X13853 mRNA. Translation: CAA32066.1 .
X05686 mRNA. Translation: CAA29171.1 .
M11728 mRNA. Translation: AAA31433.1 .
PIRi B27821. O4RBBN.
RefSeqi NP_001164592.1. NM_001171121.1.
UniGenei Ocu.1943.

3D structure databases

ProteinModelPortali P00187.
SMRi P00187. Positions 34-514.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 9986.ENSOCUP00000008665.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 100328937.

Phylogenomic databases

eggNOGi COG2124.
HOGENOMi HOG000036991.
HOVERGENi HBG106944.
InParanoidi P00187.

Family and domain databases

Gene3Di 1.10.630.10. 1 hit.
InterProi IPR001128. Cyt_P450.
IPR017972. Cyt_P450_CS.
IPR002401. Cyt_P450_E_grp-I.
IPR008066. Cyt_P450_E_grp-I_CYP1.
[Graphical view ]
Pfami PF00067. p450. 1 hit.
[Graphical view ]
PRINTSi PR00463. EP450I.
PR01683. EP450ICYP1A.
PR00385. P450.
SUPFAMi SSF48264. SSF48264. 1 hit.
PROSITEi PS00086. CYTOCHROME_P450. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "cDNA cloning and functional expression in yeast Saccharomyces cerevisiae of beta-naphthoflavone-induced rabbit liver P-450 LM4 and LM6."
    Pompon D.
    Eur. J. Biochem. 177:285-293(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], VARIANTS SER-174; SER-233 AND GLY-299.
    Strain: New Zealand white.
  2. "Structural analysis of cloned cDNAs for polycyclic hydrocarbon-inducible forms of rabbit liver microsomal cytochrome P-450."
    Kagawa N., Mihara K., Sato R.
    J. Biochem. 101:1471-1479(1987) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Liver.
  3. "Complete amino acid sequence of a cytochrome P-450 isolated from beta-naphthoflavone-induced rabbit liver microsomes. Comparison with phenobarbital-induced and constitutive isozymes and identification of invariant residues."
    Ozols J.
    J. Biol. Chem. 261:3965-3979(1986) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 2-516.
  4. "Cloning and characterization of cDNAs encoding 2,3,7,8-tetrachlorodibenzo-p-dioxin-inducible rabbit mRNAs for cytochrome P-450 isozymes 4 and 6."
    Okino S.T., Quattrochi L.C., Barnes H.J., Osanto S., Griffin K.J., Johnson E.F., Tukey R.H.
    Proc. Natl. Acad. Sci. U.S.A. 82:5310-5314(1985) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 92-515.
  5. "Modification of cytochrome P450 1A2 enzymes by the mechanism-based inactivator 2-ethynylnaphthalene and the photoaffinity label 4-azidobiphenyl."
    Yun C.H., Hammons G.J., Jones G., Martin M.V., Hopkins N.E., Alworth W.L., Guengerich F.P.
    Biochemistry 31:10556-10563(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 176-185.
  6. "On the amino acid sequence of cytochrome P-450 isozyme 4 from rabbit liver microsomes."
    Fujita V.S., Black S.D., Tarr G.E., Koop D.R., Coon M.J.
    Proc. Natl. Acad. Sci. U.S.A. 81:4260-4264(1984) [PubMed] [Europe PMC] [Abstract]
    Cited for: PARTIAL PROTEIN SEQUENCE.

Entry informationi

Entry nameiCP1A2_RABIT
AccessioniPrimary (citable) accession number: P00187
Secondary accession number(s): Q29526
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: January 23, 2007
Last modified: November 26, 2014
This is version 126 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3