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P00187

- CP1A2_RABIT

UniProt

P00187 - CP1A2_RABIT

Protein

Cytochrome P450 1A2

Gene

CYP1A2

Organism
Oryctolagus cuniculus (Rabbit)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 124 (01 Oct 2014)
      Sequence version 3 (23 Jan 2007)
      Previous versions | rss
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    Functioni

    Cytochromes P450 are a group of heme-thiolate monooxygenases. In liver microsomes, this enzyme is involved in an NADPH-dependent electron transport pathway. It oxidizes a variety of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics. Most active in catalyzing 2-hydroxylation.

    Catalytic activityi

    RH + reduced flavoprotein + O2 = ROH + oxidized flavoprotein + H2O.

    Cofactori

    Heme group.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei226 – 2261SubstrateBy similarity
    Sitei403 – 4031Involved in electron transfer with reductase
    Metal bindingi458 – 4581Iron (heme axial ligand)

    GO - Molecular functioni

    1. aromatase activity Source: UniProtKB-EC
    2. heme binding Source: UniProtKB
    3. iron ion binding Source: InterPro

    Keywords - Molecular functioni

    Monooxygenase, Oxidoreductase

    Keywords - Ligandi

    Heme, Iron, Metal-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Cytochrome P450 1A2 (EC:1.14.14.1)
    Alternative name(s):
    CYPIA2
    Cytochrome P450 isozyme 4
    Short name:
    Cytochrome P450 LM4
    Cytochrome P450-PM4
    Gene namesi
    Name:CYP1A2
    OrganismiOryctolagus cuniculus (Rabbit)
    Taxonomic identifieri9986 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresLagomorphaLeporidaeOryctolagus
    ProteomesiUP000001811: Unplaced

    Subcellular locationi

    GO - Cellular componenti

    1. endoplasmic reticulum membrane Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Endoplasmic reticulum, Membrane, Microsome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11Removed1 Publication
    Chaini2 – 516515Cytochrome P450 1A2PRO_0000051655Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi69 – 691O-linked (GlcNAc)By similarity

    Keywords - PTMi

    Glycoprotein

    Expressioni

    Inductioni

    By beta-naphthoflavone and 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD).

    Interactioni

    Protein-protein interaction databases

    STRINGi9986.ENSOCUP00000008665.

    Structurei

    3D structure databases

    ProteinModelPortaliP00187.
    SMRiP00187. Positions 34-514.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni361 – 3655Substrate binding

    Sequence similaritiesi

    Belongs to the cytochrome P450 family.Curated

    Phylogenomic databases

    eggNOGiCOG2124.
    HOGENOMiHOG000036991.
    HOVERGENiHBG106944.

    Family and domain databases

    Gene3Di1.10.630.10. 1 hit.
    InterProiIPR001128. Cyt_P450.
    IPR017972. Cyt_P450_CS.
    IPR002401. Cyt_P450_E_grp-I.
    IPR008066. Cyt_P450_E_grp-I_CYP1.
    [Graphical view]
    PfamiPF00067. p450. 1 hit.
    [Graphical view]
    PRINTSiPR00463. EP450I.
    PR01683. EP450ICYP1A.
    PR00385. P450.
    SUPFAMiSSF48264. SSF48264. 1 hit.
    PROSITEiPS00086. CYTOCHROME_P450. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P00187-1 [UniParc]FASTAAdd to Basket

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    MAMSPAAPLS VTELLLVSAV FCLVFWAVRA SRPKVPKGLK RLPGPWGWPL    50
    LGHLLTLGKN PHVALARLSR RYGDVFQIRL GSTPVVVLSG LDTIKQALVR 100
    QGDDFKGRPD LYSSSFITEG QSMTFSPDSG PVWAARRRLA QDSLKSFSIA 150
    SNPASSSSCY LEEHVSQEAE NLIGRFQELM AAVGRFDPYS QLVVSAARVI 200
    GAMCFGRRFP QGSEEMLDVV RNSSKFVETA SSGSPVDFFP ILRYLPNRPL 250
    QRFKDFNQRF LRFLQKTVRE HYEDFDRNSI QDITGALFKH SEKNSKANSG 300
    LIPQEKIVNL VNDIFGAGFD TITTALSWSL MYLVTNPRRQ RKIQEELDAV 350
    VGRARQPRLS DRPQLPYLEA FILELFRHTS FVPFTIPHST TRDTTLNGFH 400
    IPKECCIFIN QWQINHDPQL WGDPEEFRPE RFLTADGAAI NKPLSEKVTL 450
    FGLGKRRCIG ETLARWEVFL FLAILLQRLE FSVPPGVPVD LTPIYGLTMK 500
    HPRCEHVQAR PRFSDQ 516
    Length:516
    Mass (Da):58,334
    Last modified:January 23, 2007 - v3
    Checksum:i3FD074D74A47C3EF
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti22 – 221C → S AA sequence (PubMed:3949797)Curated
    Sequence conflicti67 – 671R → P in AAA31437. (PubMed:2847925)Curated
    Sequence conflicti70 – 701R → Q AA sequence (PubMed:3949797)Curated
    Sequence conflicti92 – 921D → N AA sequence (PubMed:3949797)Curated
    Sequence conflicti93 – 931T → H in AAA31433. (PubMed:2991917)Curated
    Sequence conflicti121 – 1211Q → H in CAA29171. (PubMed:3667560)Curated
    Sequence conflicti172 – 1721L → F AA sequence (PubMed:3949797)Curated
    Sequence conflicti194 – 1941V → S AA sequence (PubMed:3949797)Curated
    Sequence conflicti208 – 2125RFPQG → FPQGM AA sequence (PubMed:3949797)Curated
    Sequence conflicti208 – 2081R → H in AAA31437. (PubMed:2847925)Curated
    Sequence conflicti208 – 2081R → H in CAA32066. (PubMed:2847925)Curated
    Sequence conflicti208 – 2081R → H in AAA31433. (PubMed:2991917)Curated
    Sequence conflicti247 – 2515NRPLQ → QPNLR AA sequence (PubMed:3949797)Curated
    Sequence conflicti288 – 2881F → I in AAA31433. (PubMed:2991917)Curated
    Sequence conflicti291 – 30212SEKNS…NSGLI → NEMDSMDDGAHV in AAA31433. (PubMed:2991917)CuratedAdd
    BLAST
    Sequence conflicti309 – 3091N → T in AAA31433. (PubMed:2991917)Curated
    Sequence conflicti354 – 3552AR → PG in AAA31437. (PubMed:2847925)Curated
    Sequence conflicti354 – 3552AR → PG in CAA32066. (PubMed:2847925)Curated
    Sequence conflicti358 – 3581R → L in AAA31433. (PubMed:2991917)Curated
    Sequence conflicti359 – 3591L → I AA sequence (PubMed:3949797)Curated
    Sequence conflicti359 – 3591L → I in AAA31433. (PubMed:2991917)Curated
    Sequence conflicti462 – 4621T → I in AAA31433. (PubMed:2991917)Curated
    Sequence conflicti494 – 4941I → T AA sequence (PubMed:3949797)Curated

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti174 – 1741G → S.1 Publication
    Natural varianti233 – 2331G → S.1 Publication
    Natural varianti299 – 2991S → G.1 Publication

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M36538 mRNA. Translation: AAA31437.1.
    X13853 mRNA. Translation: CAA32066.1.
    X05686 mRNA. Translation: CAA29171.1.
    M11728 mRNA. Translation: AAA31433.1.
    PIRiB27821. O4RBBN.
    RefSeqiNP_001164592.1. NM_001171121.1.
    UniGeneiOcu.1943.

    Genome annotation databases

    GeneIDi100328937.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M36538 mRNA. Translation: AAA31437.1 .
    X13853 mRNA. Translation: CAA32066.1 .
    X05686 mRNA. Translation: CAA29171.1 .
    M11728 mRNA. Translation: AAA31433.1 .
    PIRi B27821. O4RBBN.
    RefSeqi NP_001164592.1. NM_001171121.1.
    UniGenei Ocu.1943.

    3D structure databases

    ProteinModelPortali P00187.
    SMRi P00187. Positions 34-514.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 9986.ENSOCUP00000008665.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 100328937.

    Phylogenomic databases

    eggNOGi COG2124.
    HOGENOMi HOG000036991.
    HOVERGENi HBG106944.

    Family and domain databases

    Gene3Di 1.10.630.10. 1 hit.
    InterProi IPR001128. Cyt_P450.
    IPR017972. Cyt_P450_CS.
    IPR002401. Cyt_P450_E_grp-I.
    IPR008066. Cyt_P450_E_grp-I_CYP1.
    [Graphical view ]
    Pfami PF00067. p450. 1 hit.
    [Graphical view ]
    PRINTSi PR00463. EP450I.
    PR01683. EP450ICYP1A.
    PR00385. P450.
    SUPFAMi SSF48264. SSF48264. 1 hit.
    PROSITEi PS00086. CYTOCHROME_P450. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "cDNA cloning and functional expression in yeast Saccharomyces cerevisiae of beta-naphthoflavone-induced rabbit liver P-450 LM4 and LM6."
      Pompon D.
      Eur. J. Biochem. 177:285-293(1988) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], VARIANTS SER-174; SER-233 AND GLY-299.
      Strain: New Zealand white.
    2. "Structural analysis of cloned cDNAs for polycyclic hydrocarbon-inducible forms of rabbit liver microsomal cytochrome P-450."
      Kagawa N., Mihara K., Sato R.
      J. Biochem. 101:1471-1479(1987) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Tissue: Liver.
    3. "Complete amino acid sequence of a cytochrome P-450 isolated from beta-naphthoflavone-induced rabbit liver microsomes. Comparison with phenobarbital-induced and constitutive isozymes and identification of invariant residues."
      Ozols J.
      J. Biol. Chem. 261:3965-3979(1986) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 2-516.
    4. "Cloning and characterization of cDNAs encoding 2,3,7,8-tetrachlorodibenzo-p-dioxin-inducible rabbit mRNAs for cytochrome P-450 isozymes 4 and 6."
      Okino S.T., Quattrochi L.C., Barnes H.J., Osanto S., Griffin K.J., Johnson E.F., Tukey R.H.
      Proc. Natl. Acad. Sci. U.S.A. 82:5310-5314(1985) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 92-515.
    5. "Modification of cytochrome P450 1A2 enzymes by the mechanism-based inactivator 2-ethynylnaphthalene and the photoaffinity label 4-azidobiphenyl."
      Yun C.H., Hammons G.J., Jones G., Martin M.V., Hopkins N.E., Alworth W.L., Guengerich F.P.
      Biochemistry 31:10556-10563(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 176-185.
    6. "On the amino acid sequence of cytochrome P-450 isozyme 4 from rabbit liver microsomes."
      Fujita V.S., Black S.D., Tarr G.E., Koop D.R., Coon M.J.
      Proc. Natl. Acad. Sci. U.S.A. 81:4260-4264(1984) [PubMed] [Europe PMC] [Abstract]
      Cited for: PARTIAL PROTEIN SEQUENCE.

    Entry informationi

    Entry nameiCP1A2_RABIT
    AccessioniPrimary (citable) accession number: P00187
    Secondary accession number(s): Q29526
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 21, 1986
    Last sequence update: January 23, 2007
    Last modified: October 1, 2014
    This is version 124 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3