P00186 (CP1A2_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Cytochrome P450 1A2
EC=1.14.14.1

Alternative name(s):
CYPIA2
Cytochrome P450-P2
Cytochrome P450-P3

Gene names

Name:	Cyp1a2
Synonyms:	Cyp1a-2

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	513 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Cytochromes P450 are a group of heme-thiolate monooxygenases. In liver microsomes, this enzyme is involved in an NADPH-dependent electron transport pathway. It oxidizes a variety of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics. Most active in catalyzing 2-hydroxylation By similarity.
Catalytic activity	RH + reduced flavoprotein + O2 = ROH + oxidized flavoprotein + H2O.
Cofactor	Heme group By similarity.
Subcellular location	Endoplasmic reticulum membrane; Peripheral membrane protein. Microsome membrane; Peripheral membrane protein.
Induction	By 3-methylcholanthrene (3MC).
Sequence similarities	Belongs to the cytochrome P450 family.

Ontologies

Keywords
Cellular component	Endoplasmic reticulum Membrane Microsome
Coding sequence diversity	Polymorphism
Ligand	Heme Iron Metal-binding
Molecular function	Monooxygenase Oxidoreductase
PTM	Acetylation
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	alkaloid metabolic process Inferred from electronic annotation. Source: Compara cellular respiration Inferred from mutant phenotype PubMed 7761462. Source: MGI cellular response to cadmium ion Inferred from direct assay PubMed 16183037. Source: MGI dibenzo-p-dioxin metabolic process Inferred from mutant phenotype PubMed 16636061PubMed 9240455. Source: MGI drug metabolic process Inferred from mutant phenotype PubMed 8643688. Source: MGI exogenous drug catabolic process Inferred from electronic annotation. Source: Compara hydrogen peroxide biosynthetic process Inferred from mutant phenotype PubMed 14980704. Source: MGI lung development Inferred from mutant phenotype PubMed 7761462. Source: MGI monocarboxylic acid metabolic process Inferred from electronic annotation. Source: Compara monoterpenoid metabolic process Inferred from electronic annotation. Source: Compara oxidative deethylation Inferred from electronic annotation. Source: Compara oxidative demethylation Inferred from electronic annotation. Source: Compara porphyrin-containing compound metabolic process Inferred from mutant phenotype PubMed 11274970PubMed 9461503. Source: MGI post-embryonic development Inferred from mutant phenotype PubMed 7761462. Source: MGI regulation of gene expression Inferred from mutant phenotype PubMed 12438513. Source: MGI response to estradiol stimulus Inferred from electronic annotation. Source: Compara response to lipopolysaccharide Inferred from electronic annotation. Source: Compara response to stress Inferred from electronic annotation. Source: Compara steroid catabolic process Inferred from electronic annotation. Source: Compara toxin biosynthetic process Inferred from electronic annotation. Source: Compara toxin metabolic process Inferred from mutant phenotype PubMed 17166882. Source: MGI
Cellular_component	endoplasmic reticulum membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	aromatase activity Inferred from electronic annotation. Source: EC caffeine oxidase activity Inferred from electronic annotation. Source: Compara demethylase activity Inferred from electronic annotation. Source: Compara electron carrier activity Inferred from electronic annotation. Source: InterPro heme binding Inferred from sequence or structural similarity. Source: UniProtKB iron ion binding Inferred from electronic annotation. Source: InterPro oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen Inferred from direct assay PubMed 8274012. Source: MGI
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed By similarity
	Chain	2 – 513	512	Cytochrome P450 1A2		PRO_0000051654
Sites
	Metal binding	456	1	Iron (heme axial ligand)
	Binding site	225	1	Substrate By similarity
Amino acid modifications
	Modified residue	288	1	N6-acetyllysine Ref.10
Natural variations
	Natural variant	384	1	I → M in P2.

Sequences

	Sequence		Length	Mass (Da)
	P00186 [UniParc]. Last modified July 21, 1986. Version 1. Checksum: DC2D7D976B126E3B	FASTA	513	58,184
	10 20 30 40 50 60 MAFSQYISLA PELLLATAIF CLVFWMVRAS RTQVPKGLKN PPGPWGLPFI GHMLTVGKNP 70 80 90 100 110 120 HLSLTRLSQQ YGDVLQIRIG STPVVVLSGL NTIKQALVRQ GDDFKGRPDL YSFTLITNGK 130 140 150 160 170 180 SMTFNPDSGP VWAARRRLAQ DALKSFSIAS DPTSASSCYL EEHVSKEANH LVSKLQKAMA 190 200 210 220 230 240 EVGHFEPVSQ VVESVANVIG AMCFGKNFPR KSEEMLNIVN NSKDFVENVT SGNAVDFFPV 250 260 270 280 290 300 LRYLPNPALK RFKTFNDNFV LFLQKTVQEH YQDFNKNSIQ DITSALFKHS ENYKDNGGLI 310 320 330 340 350 360 PEEKIVNIVN DIFGAGFDTV TTAITWSILL LVTWPNVQRK IHEELDTVVG RDRQPRLSDR 370 380 390 400 410 420 PQLPYLEAFI LEIYRYTSFV PFTIPHSTTR DTSLNGFHIP KERCIYINQW QVNHDEKQWK 430 440 450 460 470 480 DPFVFRPERF LTNNNSAIDK TQSEKVMLFG LGKRRCIGEI PAKWEVFLFL AILLQHLEFS 490 500 510 VPPGVKVDLT PNYGLTMKPG TCEHVQAWPR FSK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The murine Ah locus. Comparison of the complete cytochrome P1-450 and P3-450 cDNA nucleotide and amino acid sequences." Kimura S., Gonzalez F.J., Nebert D.W. J. Biol. Chem. 259:10705-10713(1984) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA] (P3). Strain: C57BL/6N.
[2]	"Mouse cytochrome P3-450: complete cDNA and amino acid sequence." Kimura S., Gonzalez F.J., Nebert D.W. Nucleic Acids Res. 12:2917-2928(1984) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA] (P3). Strain: C57BL/6N.
[3]	"Comparison of the flanking regions and introns of the mouse 2,3,7,8-tetrachlorodibenzo-p-dioxin-inducible cytochrome P1-450 and P3-450 genes." Gonzalez F.J., Kimura S., Nebert D.W. J. Biol. Chem. 260:5040-5049(1985) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA] (P3).
[4]	Erratum Gonzalez F.J., Kimura S., Nebert D.W. J. Biol. Chem. 260:11884-11889(1985)
[5]	"Isolation and characterization of full-length mouse cDNA and genomic clones of 3-methylcholanthrene-inducible cytochrome P1-450 and P3-450." Gonzalez F.J., McKenzie P.I., Kimura S., Nebert D.W. Gene 29:281-292(1984) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA] (P3).
[6]	"cDNA and complete amino acid sequence of mouse P2(450): allelic variant of mouse P3(450) gene." Kimura S., Nebert D.W. Nucleic Acids Res. 14:6765-6766(1986) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (P2). Strain: DBA/2N. Tissue: Liver.
[7]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (P3). Tissue: Liver.
[8]	"Amino-terminal sequence and structure of monoclonal antibody immunopurified cytochromes P-450." Cheng K.C., Park S.S., Krutzsch H.C., Grantham P.H., Gelboin H.V., Friedman F.K. Biochemistry 25:2397-2402(1986) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 2-26. Strain: DBA/2.
[9]	"Methylation differences in the murine P-1-450 and P-3-450 genes in wild-type and mutant hepatoma cell culture." Peterson T.C., Gonzalez F.J., Nebert D.W. Biochem. Pharmacol. 35:2107-2114(1986) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 277-315.
[10]	"Substrate and functional diversity of lysine acetylation revealed by a proteomics survey." Kim S.C., Sprung R., Chen Y., Xu Y., Ball H., Pei J., Cheng T., Kho Y., Xiao H., Xiao L., Grishin N.V., White M., Yang X.-J., Zhao Y. Mol. Cell 23:607-618(2006) [PubMed] [Europe PMC] [Abstract] Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT LYS-288, MASS SPECTROMETRY. Tissue: Liver.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X01682 Genomic DNA. Translation: CAA25837.1. X00479 mRNA. Translation: CAA25156.1. X04283 mRNA. Translation: CAA27832.1. K02589 mRNA. Translation: AAA37509.1. Sequence problems. M10022 Genomic DNA. Translation: AAA37508.1. BC018298 mRNA. Translation: AAH18298.1. BC054827 mRNA. Translation: AAH54827.1.
IPI	IPI00128287.
PIR	O4MSM3. B92495.
RefSeq	NP_034123.1. NM_009993.3.
UniGene	Mm.15537.
3D structure databases
ProteinModelPortal	P00186.
SMR	P00186. Positions 33-512.
ModBase	Search...
PTM databases
PhosphoSite	P00186.
Proteomic databases
PaxDb	P00186.
PRIDE	P00186.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000034860; ENSMUSP00000034860; ENSMUSG00000032310.
GeneID	13077.
KEGG	mmu:13077.
Organism-specific databases
CTD	1544.
MGI	MGI:88589. Cyp1a2.
Phylogenomic databases
eggNOG	COG2124.
GeneTree	ENSGT00680000099714.
HOGENOM	HOG000036991.
HOVERGEN	HBG106944.
InParanoid	P00186.
KO	K07409.
OMA	KQWKDPF.
OrthoDB	EOG4WSW9D.
Gene expression databases
ArrayExpress	P00186.
Bgee	P00186.
Genevestigator	P00186.
GermOnline	ENSMUSG00000032310. Mus musculus.
Family and domain databases
Gene3D	1.10.630.10. 1 hit.
InterPro	IPR001128. Cyt_P450. IPR017972. Cyt_P450_CS. IPR002401. Cyt_P450_E_grp-I. IPR008066. Cyt_P450_E_grp-I_CYP1. [Graphical view]
Pfam	PF00067. p450. 1 hit. [Graphical view]
PRINTS	PR00463. EP450I. PR01683. EP450ICYP1A. PR00385. P450.
SUPFAM	SSF48264. Cytochrome_P450. 1 hit.
PROSITE	PS00086. CYTOCHROME_P450. 1 hit. [Graphical view]
ProtoNet	Search...
Other
ChEMBL	CHEMBL1671611.
NextBio	283024.
SOURCE	Search...

Entry information

Entry name

CP1A2_MOUSE

Accession

Primary (citable) accession number: P00186
Secondary accession number(s): Q9QWJ4

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 21, 1986
Last sequence update:	July 21, 1986
Last modified:	April 3, 2013
This is version 126 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program