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P00186

- CP1A2_MOUSE

UniProt

P00186 - CP1A2_MOUSE

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Protein
Cytochrome P450 1A2
Gene
Cyp1a2, Cyp1a-2
Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Cytochromes P450 are a group of heme-thiolate monooxygenases. In liver microsomes, this enzyme is involved in an NADPH-dependent electron transport pathway. It oxidizes a variety of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics. Most active in catalyzing 2-hydroxylation By similarity.

Catalytic activityi

RH + reduced flavoprotein + O2 = ROH + oxidized flavoprotein + H2O.

Cofactori

Heme group By similarity.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei225 – 2251Substrate By similarity
Metal bindingi456 – 4561Iron (heme axial ligand)

GO - Molecular functioni

  1. aromatase activity Source: UniProtKB-EC
  2. caffeine oxidase activity Source: Ensembl
  3. demethylase activity Source: Ensembl
  4. heme binding Source: UniProtKB
  5. iron ion binding Source: InterPro
  6. oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen Source: MGI

GO - Biological processi

  1. alkaloid metabolic process Source: Ensembl
  2. cellular aromatic compound metabolic process Source: MGI
  3. cellular respiration Source: MGI
  4. cellular response to cadmium ion Source: MGI
  5. dibenzo-p-dioxin metabolic process Source: MGI
  6. drug metabolic process Source: MGI
  7. exogenous drug catabolic process Source: Ensembl
  8. hydrogen peroxide biosynthetic process Source: MGI
  9. lung development Source: MGI
  10. monocarboxylic acid metabolic process Source: Ensembl
  11. monoterpenoid metabolic process Source: Ensembl
  12. oxidative deethylation Source: Ensembl
  13. oxidative demethylation Source: Ensembl
  14. porphyrin-containing compound metabolic process Source: MGI
  15. post-embryonic development Source: MGI
  16. regulation of gene expression Source: MGI
  17. response to estradiol Source: Ensembl
  18. response to lipopolysaccharide Source: Ensembl
  19. steroid catabolic process Source: Ensembl
  20. toxin biosynthetic process Source: Ensembl
  21. toxin metabolic process Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Monooxygenase, Oxidoreductase

Keywords - Ligandi

Heme, Iron, Metal-binding

Enzyme and pathway databases

ReactomeiREACT_196570. Synthesis of epoxy (EET) and dihydroxyeicosatrienoic acids (DHET).
REACT_196572. Synthesis of (16-20)-hydroxyeicosatetraenoic acids (HETE).

Names & Taxonomyi

Protein namesi
Recommended name:
Cytochrome P450 1A2 (EC:1.14.14.1)
Alternative name(s):
CYPIA2
Cytochrome P450-P2
Cytochrome P450-P3
Gene namesi
Name:Cyp1a2
Synonyms:Cyp1a-2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 9

Organism-specific databases

MGIiMGI:88589. Cyp1a2.

Subcellular locationi

GO - Cellular componenti

  1. endoplasmic reticulum membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane, Microsome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed By similarity
Chaini2 – 513512Cytochrome P450 1A2
PRO_0000051654Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi68 – 681O-linked (GlcNAc) By similarity

Keywords - PTMi

Glycoprotein

Proteomic databases

MaxQBiP00186.
PaxDbiP00186.
PRIDEiP00186.

PTM databases

PhosphoSiteiP00186.

Expressioni

Inductioni

By 3-methylcholanthrene (3MC).

Gene expression databases

ArrayExpressiP00186.
BgeeiP00186.
GenevestigatoriP00186.

Interactioni

Protein-protein interaction databases

IntActiP00186. 2 interactions.
MINTiMINT-1870125.

Structurei

3D structure databases

ProteinModelPortaliP00186.
SMRiP00186. Positions 33-512.

Family & Domainsi

Sequence similaritiesi

Belongs to the cytochrome P450 family.

Phylogenomic databases

eggNOGiCOG2124.
GeneTreeiENSGT00750000117317.
HOGENOMiHOG000036991.
HOVERGENiHBG106944.
InParanoidiP00186.
KOiK07409.
OMAiAFILEIY.
OrthoDBiEOG7RBZ85.
PhylomeDBiP00186.
TreeFamiTF105095.

Family and domain databases

Gene3Di1.10.630.10. 1 hit.
InterProiIPR001128. Cyt_P450.
IPR017972. Cyt_P450_CS.
IPR002401. Cyt_P450_E_grp-I.
IPR008066. Cyt_P450_E_grp-I_CYP1.
[Graphical view]
PfamiPF00067. p450. 1 hit.
[Graphical view]
PRINTSiPR00463. EP450I.
PR01683. EP450ICYP1A.
PR00385. P450.
SUPFAMiSSF48264. SSF48264. 1 hit.
PROSITEiPS00086. CYTOCHROME_P450. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P00186-1 [UniParc]FASTAAdd to Basket

« Hide

MAFSQYISLA PELLLATAIF CLVFWMVRAS RTQVPKGLKN PPGPWGLPFI    50
GHMLTVGKNP HLSLTRLSQQ YGDVLQIRIG STPVVVLSGL NTIKQALVRQ 100
GDDFKGRPDL YSFTLITNGK SMTFNPDSGP VWAARRRLAQ DALKSFSIAS 150
DPTSASSCYL EEHVSKEANH LVSKLQKAMA EVGHFEPVSQ VVESVANVIG 200
AMCFGKNFPR KSEEMLNIVN NSKDFVENVT SGNAVDFFPV LRYLPNPALK 250
RFKTFNDNFV LFLQKTVQEH YQDFNKNSIQ DITSALFKHS ENYKDNGGLI 300
PEEKIVNIVN DIFGAGFDTV TTAITWSILL LVTWPNVQRK IHEELDTVVG 350
RDRQPRLSDR PQLPYLEAFI LEIYRYTSFV PFTIPHSTTR DTSLNGFHIP 400
KERCIYINQW QVNHDEKQWK DPFVFRPERF LTNNNSAIDK TQSEKVMLFG 450
LGKRRCIGEI PAKWEVFLFL AILLQHLEFS VPPGVKVDLT PNYGLTMKPG 500
TCEHVQAWPR FSK 513
Length:513
Mass (Da):58,184
Last modified:July 21, 1986 - v1
Checksum:iDC2D7D976B126E3B
GO

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti384 – 3841I → M in P2.

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X01682 Genomic DNA. Translation: CAA25837.1.
X00479 mRNA. Translation: CAA25156.1.
X04283 mRNA. Translation: CAA27832.1.
K02589 mRNA. Translation: AAA37509.1. Sequence problems.
M10022 Genomic DNA. Translation: AAA37508.1.
BC018298 mRNA. Translation: AAH18298.1.
BC054827 mRNA. Translation: AAH54827.1.
CCDSiCCDS23229.1.
PIRiB92495. O4MSM3.
RefSeqiNP_034123.1. NM_009993.3.
UniGeneiMm.15537.

Genome annotation databases

EnsembliENSMUST00000034860; ENSMUSP00000034860; ENSMUSG00000032310.
GeneIDi13077.
KEGGimmu:13077.
UCSCiuc009pvm.1. mouse.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X01682 Genomic DNA. Translation: CAA25837.1 .
X00479 mRNA. Translation: CAA25156.1 .
X04283 mRNA. Translation: CAA27832.1 .
K02589 mRNA. Translation: AAA37509.1 . Sequence problems.
M10022 Genomic DNA. Translation: AAA37508.1 .
BC018298 mRNA. Translation: AAH18298.1 .
BC054827 mRNA. Translation: AAH54827.1 .
CCDSi CCDS23229.1.
PIRi B92495. O4MSM3.
RefSeqi NP_034123.1. NM_009993.3.
UniGenei Mm.15537.

3D structure databases

ProteinModelPortali P00186.
SMRi P00186. Positions 33-512.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

IntActi P00186. 2 interactions.
MINTi MINT-1870125.

Chemistry

ChEMBLi CHEMBL1671611.

PTM databases

PhosphoSitei P00186.

Proteomic databases

MaxQBi P00186.
PaxDbi P00186.
PRIDEi P00186.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000034860 ; ENSMUSP00000034860 ; ENSMUSG00000032310 .
GeneIDi 13077.
KEGGi mmu:13077.
UCSCi uc009pvm.1. mouse.

Organism-specific databases

CTDi 1544.
MGIi MGI:88589. Cyp1a2.

Phylogenomic databases

eggNOGi COG2124.
GeneTreei ENSGT00750000117317.
HOGENOMi HOG000036991.
HOVERGENi HBG106944.
InParanoidi P00186.
KOi K07409.
OMAi AFILEIY.
OrthoDBi EOG7RBZ85.
PhylomeDBi P00186.
TreeFami TF105095.

Enzyme and pathway databases

Reactomei REACT_196570. Synthesis of epoxy (EET) and dihydroxyeicosatrienoic acids (DHET).
REACT_196572. Synthesis of (16-20)-hydroxyeicosatetraenoic acids (HETE).

Miscellaneous databases

NextBioi 283024.
PROi P00186.
SOURCEi Search...

Gene expression databases

ArrayExpressi P00186.
Bgeei P00186.
Genevestigatori P00186.

Family and domain databases

Gene3Di 1.10.630.10. 1 hit.
InterProi IPR001128. Cyt_P450.
IPR017972. Cyt_P450_CS.
IPR002401. Cyt_P450_E_grp-I.
IPR008066. Cyt_P450_E_grp-I_CYP1.
[Graphical view ]
Pfami PF00067. p450. 1 hit.
[Graphical view ]
PRINTSi PR00463. EP450I.
PR01683. EP450ICYP1A.
PR00385. P450.
SUPFAMi SSF48264. SSF48264. 1 hit.
PROSITEi PS00086. CYTOCHROME_P450. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The murine Ah locus. Comparison of the complete cytochrome P1-450 and P3-450 cDNA nucleotide and amino acid sequences."
    Kimura S., Gonzalez F.J., Nebert D.W.
    J. Biol. Chem. 259:10705-10713(1984) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA] (P3).
    Strain: C57BL/6N.
  2. "Mouse cytochrome P3-450: complete cDNA and amino acid sequence."
    Kimura S., Gonzalez F.J., Nebert D.W.
    Nucleic Acids Res. 12:2917-2928(1984) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA] (P3).
    Strain: C57BL/6N.
  3. "Comparison of the flanking regions and introns of the mouse 2,3,7,8-tetrachlorodibenzo-p-dioxin-inducible cytochrome P1-450 and P3-450 genes."
    Gonzalez F.J., Kimura S., Nebert D.W.
    J. Biol. Chem. 260:5040-5049(1985) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA] (P3).
  4. Erratum
    Gonzalez F.J., Kimura S., Nebert D.W.
    J. Biol. Chem. 260:11884-11889(1985)
  5. "Isolation and characterization of full-length mouse cDNA and genomic clones of 3-methylcholanthrene-inducible cytochrome P1-450 and P3-450."
    Gonzalez F.J., McKenzie P.I., Kimura S., Nebert D.W.
    Gene 29:281-292(1984) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA] (P3).
  6. "cDNA and complete amino acid sequence of mouse P2(450): allelic variant of mouse P3(450) gene."
    Kimura S., Nebert D.W.
    Nucleic Acids Res. 14:6765-6766(1986) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (P2).
    Strain: DBA/2N.
    Tissue: Liver.
  7. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (P3).
    Tissue: Liver.
  8. "Amino-terminal sequence and structure of monoclonal antibody immunopurified cytochromes P-450."
    Cheng K.C., Park S.S., Krutzsch H.C., Grantham P.H., Gelboin H.V., Friedman F.K.
    Biochemistry 25:2397-2402(1986) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 2-26.
    Strain: DBA/2.
  9. "Methylation differences in the murine P-1-450 and P-3-450 genes in wild-type and mutant hepatoma cell culture."
    Peterson T.C., Gonzalez F.J., Nebert D.W.
    Biochem. Pharmacol. 35:2107-2114(1986) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 277-315.

Entry informationi

Entry nameiCP1A2_MOUSE
AccessioniPrimary (citable) accession number: P00186
Secondary accession number(s): Q9QWJ4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: July 21, 1986
Last modified: September 3, 2014
This is version 138 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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