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Protein

Cytochrome P450 1A2

Gene

Cyp1a2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Cytochromes P450 are a group of heme-thiolate monooxygenases. In liver microsomes, this enzyme is involved in an NADPH-dependent electron transport pathway. It oxidizes a variety of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics. Most active in catalyzing 2-hydroxylation.By similarity

Catalytic activityi

RH + [reduced NADPH--hemoprotein reductase] + O2 = ROH + [oxidized NADPH--hemoprotein reductase] + H2O.By similarity

Cofactori

hemeBy similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei225SubstrateBy similarity1
Metal bindingi456Iron (heme axial ligand)1

GO - Molecular functioni

GO - Biological processi

  • alkaloid metabolic process Source: MGI
  • cellular aromatic compound metabolic process Source: MGI
  • cellular respiration Source: MGI
  • cellular response to cadmium ion Source: MGI
  • cellular response to copper ion Source: Ensembl
  • dibenzo-p-dioxin metabolic process Source: MGI
  • drug catabolic process Source: MGI
  • drug metabolic process Source: MGI
  • exogenous drug catabolic process Source: MGI
  • heterocycle metabolic process Source: MGI
  • hydrogen peroxide biosynthetic process Source: MGI
  • lung development Source: MGI
  • monocarboxylic acid metabolic process Source: MGI
  • monoterpenoid metabolic process Source: MGI
  • oxidation-reduction process Source: MGI
  • oxidative deethylation Source: MGI
  • oxidative demethylation Source: MGI
  • porphyrin-containing compound metabolic process Source: MGI
  • post-embryonic development Source: MGI
  • regulation of gene expression Source: MGI
  • response to estradiol Source: Ensembl
  • response to immobilization stress Source: Ensembl
  • response to lipopolysaccharide Source: Ensembl
  • steroid catabolic process Source: MGI
  • toxin biosynthetic process Source: MGI
  • toxin metabolic process Source: MGI

Keywordsi

Molecular functionMonooxygenase, Oxidoreductase
Biological processLipid metabolism, Steroid metabolism, Sterol metabolism
LigandHeme, Iron, Metal-binding

Enzyme and pathway databases

ReactomeiR-MMU-156581 Methylation
R-MMU-211957 Aromatic amines can be N-hydroxylated or N-dealkylated by CYP1A2
R-MMU-2142670 Synthesis of epoxy (EET) and dihydroxyeicosatrienoic acids (DHET)
R-MMU-2142816 Synthesis of (16-20)-hydroxyeicosatetraenoic acids (HETE)
R-MMU-5423646 Aflatoxin activation and detoxification

Names & Taxonomyi

Protein namesi
Recommended name:
Cytochrome P450 1A2 (EC:1.14.14.1By similarity)
Alternative name(s):
CYPIA2
Cholesterol 25-hydroxylaseBy similarity
Cytochrome P450-P2
Cytochrome P450-P3
Gene namesi
Name:Cyp1a2
Synonyms:Cyp1a-2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 9

Organism-specific databases

MGIiMGI:88589 Cyp1a2

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane, Microsome

Pathology & Biotechi

Chemistry databases

ChEMBLiCHEMBL1671611

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00000516542 – 513Cytochrome P450 1A2Add BLAST512

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi68O-linked (GlcNAc) serineBy similarity1

Keywords - PTMi

Glycoprotein

Proteomic databases

MaxQBiP00186
PaxDbiP00186
PRIDEiP00186

PTM databases

iPTMnetiP00186
PhosphoSitePlusiP00186

Expressioni

Inductioni

By 3-methylcholanthrene (3MC).

Gene expression databases

BgeeiENSMUSG00000032310
ExpressionAtlasiP00186 baseline and differential
GenevisibleiP00186 MM

Interactioni

GO - Molecular functioni

Protein-protein interaction databases

IntActiP00186, 2 interactors
MINTiP00186
STRINGi10090.ENSMUSP00000034860

Structurei

3D structure databases

ProteinModelPortaliP00186
SMRiP00186
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the cytochrome P450 family.Curated

Phylogenomic databases

eggNOGiKOG0156 Eukaryota
COG2124 LUCA
GeneTreeiENSGT00900000140831
HOGENOMiHOG000036991
HOVERGENiHBG106944
InParanoidiP00186
KOiK07409
OMAiEHHRQGM
OrthoDBiEOG091G0BT8
PhylomeDBiP00186
TreeFamiTF105095

Family and domain databases

Gene3Di1.10.630.10, 1 hit
InterProiView protein in InterPro
IPR001128 Cyt_P450
IPR017972 Cyt_P450_CS
IPR002401 Cyt_P450_E_grp-I
IPR008066 Cyt_P450_E_grp-I_CYP1
IPR036396 Cyt_P450_sf
PfamiView protein in Pfam
PF00067 p450, 1 hit
PRINTSiPR00463 EP450I
PR01683 EP450ICYP1A
PR00385 P450
SUPFAMiSSF48264 SSF48264, 1 hit
PROSITEiView protein in PROSITE
PS00086 CYTOCHROME_P450, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P00186-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAFSQYISLA PELLLATAIF CLVFWMVRAS RTQVPKGLKN PPGPWGLPFI
60 70 80 90 100
GHMLTVGKNP HLSLTRLSQQ YGDVLQIRIG STPVVVLSGL NTIKQALVRQ
110 120 130 140 150
GDDFKGRPDL YSFTLITNGK SMTFNPDSGP VWAARRRLAQ DALKSFSIAS
160 170 180 190 200
DPTSASSCYL EEHVSKEANH LVSKLQKAMA EVGHFEPVSQ VVESVANVIG
210 220 230 240 250
AMCFGKNFPR KSEEMLNIVN NSKDFVENVT SGNAVDFFPV LRYLPNPALK
260 270 280 290 300
RFKTFNDNFV LFLQKTVQEH YQDFNKNSIQ DITSALFKHS ENYKDNGGLI
310 320 330 340 350
PEEKIVNIVN DIFGAGFDTV TTAITWSILL LVTWPNVQRK IHEELDTVVG
360 370 380 390 400
RDRQPRLSDR PQLPYLEAFI LEIYRYTSFV PFTIPHSTTR DTSLNGFHIP
410 420 430 440 450
KERCIYINQW QVNHDEKQWK DPFVFRPERF LTNNNSAIDK TQSEKVMLFG
460 470 480 490 500
LGKRRCIGEI PAKWEVFLFL AILLQHLEFS VPPGVKVDLT PNYGLTMKPG
510
TCEHVQAWPR FSK
Length:513
Mass (Da):58,184
Last modified:July 21, 1986 - v1
Checksum:iDC2D7D976B126E3B
GO

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural varianti384I → M in P2. 1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X01682 Genomic DNA Translation: CAA25837.1
X00479 mRNA Translation: CAA25156.1
X04283 mRNA Translation: CAA27832.1
K02589 mRNA Translation: AAA37509.1 Sequence problems.
M10022 Genomic DNA Translation: AAA37508.1
BC018298 mRNA Translation: AAH18298.1
BC054827 mRNA Translation: AAH54827.1
CCDSiCCDS23229.1
PIRiB92495 O4MSM3
RefSeqiNP_034123.1, NM_009993.3
UniGeneiMm.15537

Genome annotation databases

EnsembliENSMUST00000034860; ENSMUSP00000034860; ENSMUSG00000032310
GeneIDi13077
KEGGimmu:13077
UCSCiuc009pvm.1 mouse

Keywords - Coding sequence diversityi

Polymorphism

Similar proteinsi

Entry informationi

Entry nameiCP1A2_MOUSE
AccessioniPrimary (citable) accession number: P00186
Secondary accession number(s): Q9QWJ4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: July 21, 1986
Last modified: March 28, 2018
This is version 164 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health