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P00186

- CP1A2_MOUSE

UniProt

P00186 - CP1A2_MOUSE

Protein

Cytochrome P450 1A2

Gene

Cyp1a2

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 139 (01 Oct 2014)
      Sequence version 1 (21 Jul 1986)
      Previous versions | rss
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    Functioni

    Cytochromes P450 are a group of heme-thiolate monooxygenases. In liver microsomes, this enzyme is involved in an NADPH-dependent electron transport pathway. It oxidizes a variety of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics. Most active in catalyzing 2-hydroxylation By similarity.By similarity

    Catalytic activityi

    RH + reduced flavoprotein + O2 = ROH + oxidized flavoprotein + H2O.

    Cofactori

    Heme group.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei225 – 2251SubstrateBy similarity
    Metal bindingi456 – 4561Iron (heme axial ligand)

    GO - Molecular functioni

    1. aromatase activity Source: UniProtKB-EC
    2. caffeine oxidase activity Source: Ensembl
    3. demethylase activity Source: Ensembl
    4. heme binding Source: UniProtKB
    5. iron ion binding Source: InterPro
    6. oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen Source: MGI

    GO - Biological processi

    1. alkaloid metabolic process Source: Ensembl
    2. cellular aromatic compound metabolic process Source: MGI
    3. cellular respiration Source: MGI
    4. cellular response to cadmium ion Source: MGI
    5. dibenzo-p-dioxin metabolic process Source: MGI
    6. drug metabolic process Source: MGI
    7. exogenous drug catabolic process Source: Ensembl
    8. hydrogen peroxide biosynthetic process Source: MGI
    9. lung development Source: MGI
    10. monocarboxylic acid metabolic process Source: Ensembl
    11. monoterpenoid metabolic process Source: Ensembl
    12. oxidative deethylation Source: Ensembl
    13. oxidative demethylation Source: Ensembl
    14. porphyrin-containing compound metabolic process Source: MGI
    15. post-embryonic development Source: MGI
    16. regulation of gene expression Source: MGI
    17. response to estradiol Source: Ensembl
    18. response to lipopolysaccharide Source: Ensembl
    19. steroid catabolic process Source: Ensembl
    20. toxin biosynthetic process Source: Ensembl
    21. toxin metabolic process Source: MGI

    Keywords - Molecular functioni

    Monooxygenase, Oxidoreductase

    Keywords - Ligandi

    Heme, Iron, Metal-binding

    Enzyme and pathway databases

    ReactomeiREACT_196570. Synthesis of epoxy (EET) and dihydroxyeicosatrienoic acids (DHET).
    REACT_196572. Synthesis of (16-20)-hydroxyeicosatetraenoic acids (HETE).

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Cytochrome P450 1A2 (EC:1.14.14.1)
    Alternative name(s):
    CYPIA2
    Cytochrome P450-P2
    Cytochrome P450-P3
    Gene namesi
    Name:Cyp1a2
    Synonyms:Cyp1a-2
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 9

    Organism-specific databases

    MGIiMGI:88589. Cyp1a2.

    Subcellular locationi

    GO - Cellular componenti

    1. endoplasmic reticulum membrane Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Endoplasmic reticulum, Membrane, Microsome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11RemovedBy similarity
    Chaini2 – 513512Cytochrome P450 1A2PRO_0000051654Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi68 – 681O-linked (GlcNAc)By similarity

    Keywords - PTMi

    Glycoprotein

    Proteomic databases

    MaxQBiP00186.
    PaxDbiP00186.
    PRIDEiP00186.

    PTM databases

    PhosphoSiteiP00186.

    Expressioni

    Inductioni

    By 3-methylcholanthrene (3MC).

    Gene expression databases

    ArrayExpressiP00186.
    BgeeiP00186.
    GenevestigatoriP00186.

    Interactioni

    Protein-protein interaction databases

    IntActiP00186. 2 interactions.
    MINTiMINT-1870125.

    Structurei

    3D structure databases

    ProteinModelPortaliP00186.
    SMRiP00186. Positions 33-512.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the cytochrome P450 family.Curated

    Phylogenomic databases

    eggNOGiCOG2124.
    GeneTreeiENSGT00750000117317.
    HOGENOMiHOG000036991.
    HOVERGENiHBG106944.
    InParanoidiP00186.
    KOiK07409.
    OMAiAFILEIY.
    OrthoDBiEOG7RBZ85.
    PhylomeDBiP00186.
    TreeFamiTF105095.

    Family and domain databases

    Gene3Di1.10.630.10. 1 hit.
    InterProiIPR001128. Cyt_P450.
    IPR017972. Cyt_P450_CS.
    IPR002401. Cyt_P450_E_grp-I.
    IPR008066. Cyt_P450_E_grp-I_CYP1.
    [Graphical view]
    PfamiPF00067. p450. 1 hit.
    [Graphical view]
    PRINTSiPR00463. EP450I.
    PR01683. EP450ICYP1A.
    PR00385. P450.
    SUPFAMiSSF48264. SSF48264. 1 hit.
    PROSITEiPS00086. CYTOCHROME_P450. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P00186-1 [UniParc]FASTAAdd to Basket

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    MAFSQYISLA PELLLATAIF CLVFWMVRAS RTQVPKGLKN PPGPWGLPFI    50
    GHMLTVGKNP HLSLTRLSQQ YGDVLQIRIG STPVVVLSGL NTIKQALVRQ 100
    GDDFKGRPDL YSFTLITNGK SMTFNPDSGP VWAARRRLAQ DALKSFSIAS 150
    DPTSASSCYL EEHVSKEANH LVSKLQKAMA EVGHFEPVSQ VVESVANVIG 200
    AMCFGKNFPR KSEEMLNIVN NSKDFVENVT SGNAVDFFPV LRYLPNPALK 250
    RFKTFNDNFV LFLQKTVQEH YQDFNKNSIQ DITSALFKHS ENYKDNGGLI 300
    PEEKIVNIVN DIFGAGFDTV TTAITWSILL LVTWPNVQRK IHEELDTVVG 350
    RDRQPRLSDR PQLPYLEAFI LEIYRYTSFV PFTIPHSTTR DTSLNGFHIP 400
    KERCIYINQW QVNHDEKQWK DPFVFRPERF LTNNNSAIDK TQSEKVMLFG 450
    LGKRRCIGEI PAKWEVFLFL AILLQHLEFS VPPGVKVDLT PNYGLTMKPG 500
    TCEHVQAWPR FSK 513
    Length:513
    Mass (Da):58,184
    Last modified:July 21, 1986 - v1
    Checksum:iDC2D7D976B126E3B
    GO

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti384 – 3841I → M in P2.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X01682 Genomic DNA. Translation: CAA25837.1.
    X00479 mRNA. Translation: CAA25156.1.
    X04283 mRNA. Translation: CAA27832.1.
    K02589 mRNA. Translation: AAA37509.1. Sequence problems.
    M10022 Genomic DNA. Translation: AAA37508.1.
    BC018298 mRNA. Translation: AAH18298.1.
    BC054827 mRNA. Translation: AAH54827.1.
    CCDSiCCDS23229.1.
    PIRiB92495. O4MSM3.
    RefSeqiNP_034123.1. NM_009993.3.
    UniGeneiMm.15537.

    Genome annotation databases

    EnsembliENSMUST00000034860; ENSMUSP00000034860; ENSMUSG00000032310.
    GeneIDi13077.
    KEGGimmu:13077.
    UCSCiuc009pvm.1. mouse.

    Keywords - Coding sequence diversityi

    Polymorphism

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X01682 Genomic DNA. Translation: CAA25837.1 .
    X00479 mRNA. Translation: CAA25156.1 .
    X04283 mRNA. Translation: CAA27832.1 .
    K02589 mRNA. Translation: AAA37509.1 . Sequence problems.
    M10022 Genomic DNA. Translation: AAA37508.1 .
    BC018298 mRNA. Translation: AAH18298.1 .
    BC054827 mRNA. Translation: AAH54827.1 .
    CCDSi CCDS23229.1.
    PIRi B92495. O4MSM3.
    RefSeqi NP_034123.1. NM_009993.3.
    UniGenei Mm.15537.

    3D structure databases

    ProteinModelPortali P00186.
    SMRi P00186. Positions 33-512.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi P00186. 2 interactions.
    MINTi MINT-1870125.

    Chemistry

    ChEMBLi CHEMBL1671611.

    PTM databases

    PhosphoSitei P00186.

    Proteomic databases

    MaxQBi P00186.
    PaxDbi P00186.
    PRIDEi P00186.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000034860 ; ENSMUSP00000034860 ; ENSMUSG00000032310 .
    GeneIDi 13077.
    KEGGi mmu:13077.
    UCSCi uc009pvm.1. mouse.

    Organism-specific databases

    CTDi 1544.
    MGIi MGI:88589. Cyp1a2.

    Phylogenomic databases

    eggNOGi COG2124.
    GeneTreei ENSGT00750000117317.
    HOGENOMi HOG000036991.
    HOVERGENi HBG106944.
    InParanoidi P00186.
    KOi K07409.
    OMAi AFILEIY.
    OrthoDBi EOG7RBZ85.
    PhylomeDBi P00186.
    TreeFami TF105095.

    Enzyme and pathway databases

    Reactomei REACT_196570. Synthesis of epoxy (EET) and dihydroxyeicosatrienoic acids (DHET).
    REACT_196572. Synthesis of (16-20)-hydroxyeicosatetraenoic acids (HETE).

    Miscellaneous databases

    NextBioi 283024.
    PROi P00186.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi P00186.
    Bgeei P00186.
    Genevestigatori P00186.

    Family and domain databases

    Gene3Di 1.10.630.10. 1 hit.
    InterProi IPR001128. Cyt_P450.
    IPR017972. Cyt_P450_CS.
    IPR002401. Cyt_P450_E_grp-I.
    IPR008066. Cyt_P450_E_grp-I_CYP1.
    [Graphical view ]
    Pfami PF00067. p450. 1 hit.
    [Graphical view ]
    PRINTSi PR00463. EP450I.
    PR01683. EP450ICYP1A.
    PR00385. P450.
    SUPFAMi SSF48264. SSF48264. 1 hit.
    PROSITEi PS00086. CYTOCHROME_P450. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The murine Ah locus. Comparison of the complete cytochrome P1-450 and P3-450 cDNA nucleotide and amino acid sequences."
      Kimura S., Gonzalez F.J., Nebert D.W.
      J. Biol. Chem. 259:10705-10713(1984) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA] (P3).
      Strain: C57BL/6N.
    2. "Mouse cytochrome P3-450: complete cDNA and amino acid sequence."
      Kimura S., Gonzalez F.J., Nebert D.W.
      Nucleic Acids Res. 12:2917-2928(1984) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA] (P3).
      Strain: C57BL/6N.
    3. "Comparison of the flanking regions and introns of the mouse 2,3,7,8-tetrachlorodibenzo-p-dioxin-inducible cytochrome P1-450 and P3-450 genes."
      Gonzalez F.J., Kimura S., Nebert D.W.
      J. Biol. Chem. 260:5040-5049(1985) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA] (P3).
    4. Erratum
      Gonzalez F.J., Kimura S., Nebert D.W.
      J. Biol. Chem. 260:11884-11889(1985)
    5. "Isolation and characterization of full-length mouse cDNA and genomic clones of 3-methylcholanthrene-inducible cytochrome P1-450 and P3-450."
      Gonzalez F.J., McKenzie P.I., Kimura S., Nebert D.W.
      Gene 29:281-292(1984) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA] (P3).
    6. "cDNA and complete amino acid sequence of mouse P2(450): allelic variant of mouse P3(450) gene."
      Kimura S., Nebert D.W.
      Nucleic Acids Res. 14:6765-6766(1986) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (P2).
      Strain: DBA/2N.
      Tissue: Liver.
    7. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (P3).
      Tissue: Liver.
    8. "Amino-terminal sequence and structure of monoclonal antibody immunopurified cytochromes P-450."
      Cheng K.C., Park S.S., Krutzsch H.C., Grantham P.H., Gelboin H.V., Friedman F.K.
      Biochemistry 25:2397-2402(1986) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 2-26.
      Strain: DBA/2.
    9. "Methylation differences in the murine P-1-450 and P-3-450 genes in wild-type and mutant hepatoma cell culture."
      Peterson T.C., Gonzalez F.J., Nebert D.W.
      Biochem. Pharmacol. 35:2107-2114(1986) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 277-315.

    Entry informationi

    Entry nameiCP1A2_MOUSE
    AccessioniPrimary (citable) accession number: P00186
    Secondary accession number(s): Q9QWJ4
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 21, 1986
    Last sequence update: July 21, 1986
    Last modified: October 1, 2014
    This is version 139 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3