Reviewed,
UniProtKB/Swiss-Prot P00185 (CP1A1_RAT)
Last modified
June 16, 2009.
Version 94.
History...
Clusters with 100%,
90%,
50% identity |
Documents (1) |
Third-party data |
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Names and origin
| Protein names | Recommended name: Cytochrome P450 1A1 Short name=CYP1A1 EC=1.14.14.1 Alternative name(s): CYPIA1 P450-C P450MT2 Cleaved into the following 2 chains: 1- Recommended name: Cytochrome P450MT2A 2- Recommended name: Cytochrome P450MT2B | ||||
| Gene names |
| ||||
| Organism | Rattus norvegicus (Rat) | ||||
| Taxonomic identifier | 10116 [NCBI] | ||||
| Taxonomic lineage | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus |
Protein attributes
| Sequence length | 524 AA. |
| Sequence status | Complete. |
| Sequence processing | The displayed sequence is further processed into a mature form. |
| Protein existence | Evidence at protein level. |
General annotation (Comments)
| Function | Cytochromes P450 are a group of heme-thiolate monooxygenases. In liver microsomes, this enzyme is involved in an NADPH-dependent electron transport pathway. It oxidizes a variety of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics. |
| Catalytic activity | RH + reduced flavoprotein + O2 = ROH + oxidized flavoprotein + H2O. |
| Cofactor | Heme group By similarity. |
| Subcellular location | Cytochrome P450 1A1: Cytoplasm. Ref.7 Cytochrome P450MT2A: Endoplasmic reticulum membrane. Mitochondrion membrane. Microsome membrane. Ref.7 Cytochrome P450MT2B: Endoplasmic reticulum. Mitochondrion. Ref.7 |
| Tissue specificity | Liver. |
| Induction | By 3-methylcholanthrene (3MC) and beta-naphthoflavone (BNF). |
| Domain | Contains a chimeric signal that facilitates targeting of the protein to both the endoplasmic reticulum and mitochondria. A 12 amino acid sequence between 33 and 44 functions as a putative mitochondrial-targeting signal. The removal of the first 4- or 32-amino acid residues from the intact protein positions the mitochondrial targeting signal for efficient binding to the mitochondrial import receptors. The membrane-free P4501A1 seems to be more sensible to proteolysis. |
| Post-translational modification | Two forms; MT2A (long form) and MT2B (short form); are produced by NH2-terminal proteolytic cleavage. This cleavage activates a cryptic mitochondrial targeting signal. |
| Sequence similarities | Belongs to the cytochrome P450 family. |
Ontologies
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Chain | 2 – 524 | 523 | Cytochrome P450 1A1 | PRO_0000003564 | |||||
| Chain | 5 – 524 | 520 | Cytochrome P450MT2A | PRO_0000003565 | |||||
| Chain | 33 – 524 | 492 | Cytochrome P450MT2B | PRO_0000003566 | |||||
Regions | |||||||||
| Region | 33 – 44 | 12 | Mitochondrial targeting signal | ||||||
Sites | |||||||||
| Metal binding | 461 | 1 | Iron (heme axial ligand) | ||||||
Amino acid modifications | |||||||||
| Modified residue | 364 | 1 | Phosphoserine By similarity | ||||||
Experimental info | |||||||||
| Mutagenesis | 32 – 33 | 2 | VT → AI: No proteolytic cleavage. | ||||||
| Sequence conflict | 21 – 22 | 2 | TT → VV AA sequence Ref.5 | ||||||
| Sequence conflict | 53 | 1 | I → M in CAA25153. Ref.2 | ||||||
| Sequence conflict | 494 | 1 | M → S in AAA41025. Ref.3 | ||||||
Sequences
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References
| [1] | "Distinct organization of methylcholanthrene- and phenobarbital-inducible cytochrome P-450 genes in the rat." Sogawa K., Gotoh O., Kawajiri K., Fujii-Kuriyama Y. Proc. Natl. Acad. Sci. U.S.A. 81:5066-5070(1984) [PubMed: 6089174] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. |
| [2] | "Nucleotide sequence of a full-length cDNA coding for 3-methylcholanthrene-induced rat liver cytochrome P-450MC." Yabusaki Y., Shimizu M., Murakami H., Nakamura K., Oeda K., Ohkawa H. Nucleic Acids Res. 12:2929-2938(1984) [PubMed: 6324135] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. |
| [3] | "Gene structure and nucleotide sequence for rat cytochrome P-450c." Hines R.N., Levy J.B., Conrad R.D., Iversen P.L., Shen M.-L., Renli A.M., Bresnick E. Arch. Biochem. Biophys. 237:465-476(1985) [PubMed: 3838427] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. |
| [4] | "Amino-terminal sequence and structure of monoclonal antibody immunopurified cytochromes P-450." Cheng K.C., Park S.S., Krutzsch H.C., Grantham P.H., Gelboin H.V., Friedman F.K. Biochemistry 25:2397-2402(1986) [PubMed: 3718958] [Abstract] Cited for: PROTEIN SEQUENCE OF 2-26. |
| [5] | "Effect of nutritional imbalances on cytochrome P-450 isozymes in rat liver." Amelizad Z., Narbonne J.F., Wolf C.R., Robertson L.W., Oesch F. Biochem. Pharmacol. 37:3245-3249(1988) [PubMed: 3041969] [Abstract] Cited for: PROTEIN SEQUENCE OF 2-22. |
| [6] | "Identification and characterization of cytochrome P4501A1 amino acid residues interacting with a radiolabeled photoaffinity diazido-benzphetamine analogue." Cvrk T., Hodek P., Strobel H.W. Arch. Biochem. Biophys. 330:142-152(1996) [PubMed: 8651689] [Abstract] Cited for: PARTIAL PROTEIN SEQUENCE. |
| [7] | "Targeting of NH2-terminal-processed microsomal protein to mitochondria: a novel pathway for the biogenesis of hepatic mitochondrial P450MT2." Addya S., Anandatheerthavarada H.K., Biswas G., Bhagwat S.V., Mullick J., Avadhani N.G. J. Cell Biol. 139:589-599(1997) [PubMed: 9348277] [Abstract] Cited for: SUBCELLULAR LOCATION. Strain: Sprague-Dawley. Tissue: Liver. |
Cross-references
Sequence databases | |
|---|---|
| K02246 Genomic DNA. Translation: AAA41027.1. X00469 mRNA. Translation: CAA25153.1. M26129 Genomic DNA. Translation: AAA41025.1. | |
| IPI | IPI00206995. |
| PIR | O4RTMC. A00185. |
| RefSeq | NP_036672.2. |
| UniGene | Rn.10352 |
3D structure databases | |
| HSSP | HSSP built from PDB template 1N6B based on UniProtKB P00179. |
| ModBase | Search... |
Genome annotation databases | |
| Ensembl | ENSRNOG00000019500. Rattus norvegicus. [Contig view] |
| GeneID | 24296. |
| KEGG | rno:24296. |
Organism-specific databases | |
| RGD | 2458. Cyp1a1. |
Phylogenomic databases | |
| HOVERGEN | P00185. |
| OMA | P00185. KEHYRTF. |
Enzyme and pathway databases | |
| BRENDA | 1.14.14.1. 248. |
Gene expression databases | |
| ArrayExpress | P00185. |
| GermOnline | ENSRNOG00000019500. Rattus norvegicus. |
Family and domain databases | |
| InterPro | IPR001128. Cyt_P450. IPR017973. Cyt_P450_C. IPR017972. Cyt_P450_CS. IPR002401. Cyt_P450_E_grp-I. IPR008066. Cyt_P450_E_grp-I_CYP1. [Graphical view] |
| Gene3D | G3DSA:1.10.630.10. Cyt_P450. 1 hit. |
| PANTHER | PTHR19383. Cyt_P450. 1 hit. PTHR19383:SF63. Cyt_P450_E_grp-I_CYP1. 1 hit. |
| Pfam | PF00067. p450. 1 hit. [Graphical view] |
| PRINTS | PR00463. EP450I. PR01683. EP450ICYP1A. PR00385. P450. |
| PROSITE | PS00086. CYTOCHROME_P450. 1 hit. [Graphical view] |
| ProtoNet | Search... |
Other Resources | |
| NextBio | 602902. |
Entry information
| Entry name | CP1A1_RAT | ||||||||
| Accession | Primary (citable) accession number: P00185 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation project | HPI (Human Proteome Initiative) | ||||||||

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