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Protein

Cytochrome P450 1A1

Gene

Cyp1a1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Cytochromes P450 are a group of heme-thiolate monooxygenases. In liver microsomes, this enzyme is involved in an NADPH-dependent electron transport pathway. It oxidizes a variety of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics.

Catalytic activityi

RH + reduced flavoprotein + O2 = ROH + oxidized flavoprotein + H2O.

Cofactori

hemeBy similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei228 – 2281SubstrateBy similarity
Metal bindingi461 – 4611Iron (heme axial ligand)By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Monooxygenase, Oxidoreductase

Keywords - Ligandi

Heme, Iron, Metal-binding

Enzyme and pathway databases

ReactomeiREACT_274299. Xenobiotics.
REACT_295859. Synthesis of epoxy (EET) and dihydroxyeicosatrienoic acids (DHET).
REACT_300263. Synthesis of (16-20)-hydroxyeicosatetraenoic acids (HETE).

Names & Taxonomyi

Protein namesi
Recommended name:
Cytochrome P450 1A1 (EC:1.14.14.1)
Alternative name(s):
CYPIA1
Cytochrome P450-P1
Gene namesi
Name:Cyp1a1
Synonyms:Cyp1a-1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589 Componenti: Chromosome 9

Organism-specific databases

MGIiMGI:88588. Cyp1a1.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane, Microsome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 524524Cytochrome P450 1A1PRO_0000051631Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi71 – 711O-linked (GlcNAc)By similarity

Keywords - PTMi

Glycoprotein

Proteomic databases

MaxQBiP00184.
PaxDbiP00184.
PRIDEiP00184.

PTM databases

PhosphoSiteiP00184.

Expressioni

Inductioni

By 3-methylcholanthrene (3MC).

Gene expression databases

BgeeiP00184.
ExpressionAtlasiP00184. baseline and differential.
GenevisibleiP00184. MM.

Interactioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000034865.

Structurei

3D structure databases

ProteinModelPortaliP00184.
SMRiP00184. Positions 40-516.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the cytochrome P450 family.Curated

Phylogenomic databases

eggNOGiCOG2124.
HOGENOMiHOG000036991.
HOVERGENiHBG106944.
InParanoidiP00184.
KOiK07408.
OrthoDBiEOG7RBZ85.
PhylomeDBiP00184.
TreeFamiTF105095.

Family and domain databases

Gene3Di1.10.630.10. 1 hit.
InterProiIPR001128. Cyt_P450.
IPR017972. Cyt_P450_CS.
IPR002401. Cyt_P450_E_grp-I.
IPR008066. Cyt_P450_E_grp-I_CYP1.
[Graphical view]
PfamiPF00067. p450. 1 hit.
[Graphical view]
PRINTSiPR00463. EP450I.
PR01683. EP450ICYP1A.
PR00385. P450.
SUPFAMiSSF48264. SSF48264. 1 hit.
PROSITEiPS00086. CYTOCHROME_P450. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P00184-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPSMYGLPAF VSATELLLAV TVFCLGFWVV RATRTWVPKG LKTPPGPWGL
60 70 80 90 100
PFIGHMLTVG KNPHLSLTRL SQQYGDVLQI RIGSTPVVVL SGLNTIKQAL
110 120 130 140 150
VRQGDDFKGR PDLYSFTLIT NGKSMTFNPD SGPVWAARRR LAQNALKSFS
160 170 180 190 200
IASDPTSASS CYLEEHVSKE ANYLVSKLQK VMAEVGHFDP YKYLVVSVAN
210 220 230 240 250
VICAICFGQR YDHDDQELLS IVNLSNEFGE VTGSGYPADF IPVLRYLPNS
260 270 280 290 300
SLDAFKDLND KFYSFMKKLI KEHYRTFEKG HIRDITDSLI EHCQDRKLDE
310 320 330 340 350
NANVQLSDDK VITIVLDLFG AGFDTVTTAI SWSLMYLVTN PRVQRKIQEE
360 370 380 390 400
LDTVIGRDRQ PRLSDRPQLP YLEAFILETF RHSSFVPFTI PHSTTRDTSL
410 420 430 440 450
NGFYIPKGCC VFVNQWQVNH DRELWGDPNE FRPERFLTPS GTLDKRLSEK
460 470 480 490 500
VTLFGLGKRK CIGETIGRSE VFLFLAILLQ QIEFKVSPGE KVDMTPTYGL
510 520
TLKHARCEHF QVQMRSSGPQ HLQA
Length:524
Mass (Da):59,230
Last modified:February 1, 1996 - v2
Checksum:iE31D9E9CBA7B2B02
GO

Sequence cautioni

The sequence AAA37506.1 differs from that shown. Reason: Erroneous initiation. Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y00071 mRNA. Translation: CAA68277.1.
X01681 Genomic DNA. Translation: CAA25836.1.
K02588 mRNA. Translation: AAA37506.1. Different initiation.
M10021 Genomic DNA. Translation: AAA37507.1.
AK005000 mRNA. Translation: BAB23734.1.
M25623 Genomic DNA. Translation: AAA39868.1.
CCDSiCCDS23230.1.
PIRiA23923. O4MSM1.
RefSeqiNP_001129531.1. NM_001136059.2.
NP_034122.1. NM_009992.4.
XP_006510873.1. XM_006510810.2.
UniGeneiMm.14089.

Genome annotation databases

EnsembliENSMUST00000034865; ENSMUSP00000034865; ENSMUSG00000032315.
GeneIDi13076.
KEGGimmu:13076.
UCSCiuc009pvn.3. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y00071 mRNA. Translation: CAA68277.1.
X01681 Genomic DNA. Translation: CAA25836.1.
K02588 mRNA. Translation: AAA37506.1. Different initiation.
M10021 Genomic DNA. Translation: AAA37507.1.
AK005000 mRNA. Translation: BAB23734.1.
M25623 Genomic DNA. Translation: AAA39868.1.
CCDSiCCDS23230.1.
PIRiA23923. O4MSM1.
RefSeqiNP_001129531.1. NM_001136059.2.
NP_034122.1. NM_009992.4.
XP_006510873.1. XM_006510810.2.
UniGeneiMm.14089.

3D structure databases

ProteinModelPortaliP00184.
SMRiP00184. Positions 40-516.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000034865.

Chemistry

ChEMBLiCHEMBL6171.

PTM databases

PhosphoSiteiP00184.

Proteomic databases

MaxQBiP00184.
PaxDbiP00184.
PRIDEiP00184.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000034865; ENSMUSP00000034865; ENSMUSG00000032315.
GeneIDi13076.
KEGGimmu:13076.
UCSCiuc009pvn.3. mouse.

Organism-specific databases

CTDi1543.
MGIiMGI:88588. Cyp1a1.

Phylogenomic databases

eggNOGiCOG2124.
HOGENOMiHOG000036991.
HOVERGENiHBG106944.
InParanoidiP00184.
KOiK07408.
OrthoDBiEOG7RBZ85.
PhylomeDBiP00184.
TreeFamiTF105095.

Enzyme and pathway databases

ReactomeiREACT_274299. Xenobiotics.
REACT_295859. Synthesis of epoxy (EET) and dihydroxyeicosatrienoic acids (DHET).
REACT_300263. Synthesis of (16-20)-hydroxyeicosatetraenoic acids (HETE).

Miscellaneous databases

NextBioi283020.
PROiP00184.
SOURCEiSearch...

Gene expression databases

BgeeiP00184.
ExpressionAtlasiP00184. baseline and differential.
GenevisibleiP00184. MM.

Family and domain databases

Gene3Di1.10.630.10. 1 hit.
InterProiIPR001128. Cyt_P450.
IPR017972. Cyt_P450_CS.
IPR002401. Cyt_P450_E_grp-I.
IPR008066. Cyt_P450_E_grp-I_CYP1.
[Graphical view]
PfamiPF00067. p450. 1 hit.
[Graphical view]
PRINTSiPR00463. EP450I.
PR01683. EP450ICYP1A.
PR00385. P450.
SUPFAMiSSF48264. SSF48264. 1 hit.
PROSITEiPS00086. CYTOCHROME_P450. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The murine Ah locus. Comparison of the complete cytochrome P1-450 and P3-450 cDNA nucleotide and amino acid sequences."
    Kimura S., Gonzalez F.J., Nebert D.W.
    J. Biol. Chem. 259:10705-10713(1984) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA].
    Strain: C57BL/6.
    Tissue: Liver.
  2. "Comparison of the flanking regions and introns of the mouse 2,3,7,8-tetrachlorodibenzo-p-dioxin-inducible cytochrome P1-450 and P3-450 genes."
    Gonzalez F.J., Kimura S., Nebert D.W.
    J. Biol. Chem. 260:5040-5049(1985) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  3. "Analysis of two benzo[a]pyrene-resistant mutants of the mouse hepatoma Hepa-1 P(1)450 gene via cDNA expression in yeast."
    Kimura S., Smith H.H., Hankinson O., Nebert D.W.
    EMBO J. 6:1929-1933(1987) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: C57BL/6.
  4. "Isolation and characterization of full-length mouse cDNA and genomic clones of 3-methylcholanthrene-inducible cytochrome P1-450 and P3-450."
    Gonzalez F.J., McKenzie P.I., Kimura S., Nebert D.W.
    Gene 29:281-292(1984) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  5. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J.
    Tissue: Liver.
  6. "Amino-terminal sequence and structure of monoclonal antibody immunopurified cytochromes P-450."
    Cheng K.C., Park S.S., Krutzsch H.C., Grantham P.H., Gelboin H.V., Friedman F.K.
    Biochemistry 25:2397-2402(1986) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 2-25.
    Strain: C57BL/6.
  7. "Methylation differences in the murine P-1-450 and P-3-450 genes in wild-type and mutant hepatoma cell culture."
    Peterson T.C., Gonzalez F.J., Nebert D.W.
    Biochem. Pharmacol. 35:2107-2114(1986) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 280-321.

Entry informationi

Entry nameiCP1A1_MOUSE
AccessioniPrimary (citable) accession number: P00184
Secondary accession number(s): Q61455
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: February 1, 1996
Last modified: June 24, 2015
This is version 148 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.