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P00184 (CP1A1_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 139. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Cytochrome P450 1A1

EC=1.14.14.1
Alternative name(s):
CYPIA1
Cytochrome P450-P1
Gene names
Name:Cyp1a1
Synonyms:Cyp1a-1
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length524 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Cytochromes P450 are a group of heme-thiolate monooxygenases. In liver microsomes, this enzyme is involved in an NADPH-dependent electron transport pathway. It oxidizes a variety of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics.

Catalytic activity

RH + reduced flavoprotein + O2 = ROH + oxidized flavoprotein + H2O.

Cofactor

Heme group By similarity.

Subcellular location

Endoplasmic reticulum membrane; Peripheral membrane protein. Microsome membrane; Peripheral membrane protein.

Induction

By 3-methylcholanthrene (3MC).

Sequence similarities

Belongs to the cytochrome P450 family.

Sequence caution

The sequence AAA37506.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
   Cellular componentEndoplasmic reticulum
Membrane
Microsome
   LigandHeme
Iron
Metal-binding
   Molecular functionMonooxygenase
Oxidoreductase
   Technical termComplete proteome
Direct protein sequencing
Reference proteome
Gene Ontology (GO)
   Biological_process9-cis-retinoic acid biosynthetic process

Inferred from electronic annotation. Source: Ensembl

aging

Inferred from electronic annotation. Source: Ensembl

amine metabolic process

Inferred from mutant phenotype PubMed 17052995. Source: MGI

camera-type eye development

Inferred from electronic annotation. Source: Ensembl

cell proliferation

Inferred from electronic annotation. Source: Ensembl

cellular response to organic cyclic compound

Inferred from sequence orthology PubMed 23275542. Source: MGI

coumarin metabolic process

Inferred from electronic annotation. Source: Ensembl

digestive tract development

Inferred from electronic annotation. Source: Ensembl

drug metabolic process

Inferred from electronic annotation. Source: Ensembl

embryo development ending in birth or egg hatching

Inferred from electronic annotation. Source: Ensembl

flavonoid metabolic process

Inferred from electronic annotation. Source: Ensembl

hepatocyte differentiation

Inferred from electronic annotation. Source: Ensembl

heterocycle metabolic process

Inferred from mutant phenotype PubMed 17052995. Source: MGI

hydrogen peroxide biosynthetic process

Inferred from mutant phenotype PubMed 14980704. Source: MGI

insecticide metabolic process

Inferred from electronic annotation. Source: Ensembl

maternal process involved in parturition

Inferred from electronic annotation. Source: Ensembl

porphyrin-containing compound metabolic process

Inferred from electronic annotation. Source: Ensembl

positive regulation of G1/S transition of mitotic cell cycle

Inferred from electronic annotation. Source: Ensembl

response to antibiotic

Inferred from electronic annotation. Source: Ensembl

response to arsenic-containing substance

Inferred from electronic annotation. Source: Ensembl

response to drug

Inferred from electronic annotation. Source: Ensembl

response to food

Inferred from electronic annotation. Source: Ensembl

response to herbicide

Inferred from electronic annotation. Source: Ensembl

response to hyperoxia

Inferred from electronic annotation. Source: Ensembl

response to hypoxia

Inferred from electronic annotation. Source: Ensembl

response to iron(III) ion

Inferred from electronic annotation. Source: Ensembl

response to lipopolysaccharide

Inferred from electronic annotation. Source: Ensembl

response to nematode

Inferred from electronic annotation. Source: Ensembl

response to toxic substance

Inferred from mutant phenotype PubMed 16377763. Source: MGI

response to virus

Inferred from electronic annotation. Source: Ensembl

response to vitamin A

Inferred from electronic annotation. Source: Ensembl

response to wounding

Inferred from electronic annotation. Source: Ensembl

toxin metabolic process

Inferred from mutant phenotype PubMed 17166882. Source: MGI

   Cellular_componentendoplasmic reticulum membrane

Inferred from electronic annotation. Source: UniProtKB-SubCell

mitochondrion

Inferred from electronic annotation. Source: Ensembl

   Molecular_functionaromatase activity

Inferred from electronic annotation. Source: UniProtKB-EC

demethylase activity

Inferred from electronic annotation. Source: Ensembl

flavonoid 3'-monooxygenase activity

Inferred from electronic annotation. Source: Ensembl

heme binding

Inferred from electronic annotation. Source: InterPro

iron ion binding

Inferred from electronic annotation. Source: InterPro

monooxygenase activity

Inferred from direct assay PubMed 14642533. Source: MGI

oxidoreductase activity, acting on diphenols and related substances as donors

Inferred from electronic annotation. Source: Ensembl

oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen

Inferred from direct assay PubMed 8274012. Source: MGI

steroid hydroxylase activity

Inferred from electronic annotation. Source: Ensembl

vitamin D 24-hydroxylase activity

Inferred from electronic annotation. Source: Ensembl

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 524524Cytochrome P450 1A1
PRO_0000051631

Sites

Metal binding4611Iron (heme axial ligand) By similarity
Binding site2281Substrate By similarity

Sequences

Sequence LengthMass (Da)Tools
P00184 [UniParc].

Last modified February 1, 1996. Version 2.
Checksum: E31D9E9CBA7B2B02

FASTA52459,230
        10         20         30         40         50         60 
MPSMYGLPAF VSATELLLAV TVFCLGFWVV RATRTWVPKG LKTPPGPWGL PFIGHMLTVG 

        70         80         90        100        110        120 
KNPHLSLTRL SQQYGDVLQI RIGSTPVVVL SGLNTIKQAL VRQGDDFKGR PDLYSFTLIT 

       130        140        150        160        170        180 
NGKSMTFNPD SGPVWAARRR LAQNALKSFS IASDPTSASS CYLEEHVSKE ANYLVSKLQK 

       190        200        210        220        230        240 
VMAEVGHFDP YKYLVVSVAN VICAICFGQR YDHDDQELLS IVNLSNEFGE VTGSGYPADF 

       250        260        270        280        290        300 
IPVLRYLPNS SLDAFKDLND KFYSFMKKLI KEHYRTFEKG HIRDITDSLI EHCQDRKLDE 

       310        320        330        340        350        360 
NANVQLSDDK VITIVLDLFG AGFDTVTTAI SWSLMYLVTN PRVQRKIQEE LDTVIGRDRQ 

       370        380        390        400        410        420 
PRLSDRPQLP YLEAFILETF RHSSFVPFTI PHSTTRDTSL NGFYIPKGCC VFVNQWQVNH 

       430        440        450        460        470        480 
DRELWGDPNE FRPERFLTPS GTLDKRLSEK VTLFGLGKRK CIGETIGRSE VFLFLAILLQ 

       490        500        510        520 
QIEFKVSPGE KVDMTPTYGL TLKHARCEHF QVQMRSSGPQ HLQA 

« Hide

References

« Hide 'large scale' references
[1]"The murine Ah locus. Comparison of the complete cytochrome P1-450 and P3-450 cDNA nucleotide and amino acid sequences."
Kimura S., Gonzalez F.J., Nebert D.W.
J. Biol. Chem. 259:10705-10713(1984) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA].
Strain: C57BL/6.
Tissue: Liver.
[2]"Comparison of the flanking regions and introns of the mouse 2,3,7,8-tetrachlorodibenzo-p-dioxin-inducible cytochrome P1-450 and P3-450 genes."
Gonzalez F.J., Kimura S., Nebert D.W.
J. Biol. Chem. 260:5040-5049(1985) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]"Analysis of two benzo[a]pyrene-resistant mutants of the mouse hepatoma Hepa-1 P(1)450 gene via cDNA expression in yeast."
Kimura S., Smith H.H., Hankinson O., Nebert D.W.
EMBO J. 6:1929-1933(1987) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: C57BL/6.
[4]"Isolation and characterization of full-length mouse cDNA and genomic clones of 3-methylcholanthrene-inducible cytochrome P1-450 and P3-450."
Gonzalez F.J., McKenzie P.I., Kimura S., Nebert D.W.
Gene 29:281-292(1984) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[5]"The transcriptional landscape of the mammalian genome."
Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. expand/collapse author list , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: C57BL/6J.
Tissue: Liver.
[6]"Amino-terminal sequence and structure of monoclonal antibody immunopurified cytochromes P-450."
Cheng K.C., Park S.S., Krutzsch H.C., Grantham P.H., Gelboin H.V., Friedman F.K.
Biochemistry 25:2397-2402(1986) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 2-25.
Strain: C57BL/6.
[7]"Methylation differences in the murine P-1-450 and P-3-450 genes in wild-type and mutant hepatoma cell culture."
Peterson T.C., Gonzalez F.J., Nebert D.W.
Biochem. Pharmacol. 35:2107-2114(1986) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 280-321.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
Y00071 mRNA. Translation: CAA68277.1.
X01681 Genomic DNA. Translation: CAA25836.1.
K02588 mRNA. Translation: AAA37506.1. Different initiation.
M10021 Genomic DNA. Translation: AAA37507.1.
AK005000 mRNA. Translation: BAB23734.1.
M25623 Genomic DNA. Translation: AAA39868.1.
CCDSCCDS23230.1.
PIRO4MSM1. A23923.
RefSeqNP_001129531.1. NM_001136059.2.
NP_034122.1. NM_009992.4.
XP_006510873.1. XM_006510810.1.
UniGeneMm.14089.

3D structure databases

ProteinModelPortalP00184.
SMRP00184. Positions 40-516.
ModBaseSearch...
MobiDBSearch...

Chemistry

ChEMBLCHEMBL6171.

PTM databases

PhosphoSiteP00184.

Proteomic databases

PaxDbP00184.
PRIDEP00184.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000034865; ENSMUSP00000034865; ENSMUSG00000032315.
GeneID13076.
KEGGmmu:13076.
UCSCuc009pvn.3. mouse.

Organism-specific databases

CTD1543.
MGIMGI:88588. Cyp1a1.

Phylogenomic databases

eggNOGCOG2124.
HOGENOMHOG000036991.
HOVERGENHBG106944.
InParanoidP00184.
KOK07408.
OMAENANIQV.
OrthoDBEOG7RBZ85.
PhylomeDBP00184.
TreeFamTF105095.

Gene expression databases

ArrayExpressP00184.
BgeeP00184.
GenevestigatorP00184.

Family and domain databases

Gene3D1.10.630.10. 1 hit.
InterProIPR001128. Cyt_P450.
IPR017972. Cyt_P450_CS.
IPR002401. Cyt_P450_E_grp-I.
IPR008066. Cyt_P450_E_grp-I_CYP1.
[Graphical view]
PfamPF00067. p450. 1 hit.
[Graphical view]
PRINTSPR00463. EP450I.
PR01683. EP450ICYP1A.
PR00385. P450.
SUPFAMSSF48264. SSF48264. 1 hit.
PROSITEPS00086. CYTOCHROME_P450. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio283020.
PROP00184.
SOURCESearch...

Entry information

Entry nameCP1A1_MOUSE
AccessionPrimary (citable) accession number: P00184
Secondary accession number(s): Q61455
Entry history
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: February 1, 1996
Last modified: July 9, 2014
This is version 139 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot