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Protein

Camphor 5-monooxygenase

Gene

camC

Organism
Pseudomonas putida (Arthrobacter siderocapsulatus)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Involved in a camphor oxidation system.

Catalytic activityi

+-camphor + reduced putidaredoxin + O2 = (+)-exo-5-hydroxycamphor + oxidized putidaredoxin + H2O.

Cofactori

Pathwayi: (R)-camphor degradation

This protein is involved in the pathway (R)-camphor degradation, which is part of Terpene metabolism.
View all proteins of this organism that are known to be involved in the pathway (R)-camphor degradation and in Terpene metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi358Iron (heme axial ligand)1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionMonooxygenase, Oxidoreductase
LigandHeme, Iron, Metal-binding

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-3021.
BRENDAi1.14.15.1. 5092.
UniPathwayiUPA00719.

Names & Taxonomyi

Protein namesi
Recommended name:
Camphor 5-monooxygenase (EC:1.14.15.1)
Alternative name(s):
Cytochrome P450-cam
Short name:
Cytochrome P450cam
Gene namesi
Name:camC
Synonyms:cyp101
OrganismiPseudomonas putida (Arthrobacter siderocapsulatus)
Taxonomic identifieri303 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Chemistry databases

ChEMBLiCHEMBL5594.
DrugBankiDB02817. 5-Exo-Hydroxycamphor.
DB03627. Adamantane.
DB04032. Adamantane-1-Carboxylic Acid-5-Dimethylamino-Naphthalene-1-Sulfonylamino-Butyl-Amide.
DB03031. Adamantane-1-Carboxylic Acid-5-Dimethylamino-Naphthalene-1-Sulfonylamino-Octyl-Amide.
DB02125. Adamantanone.
DB04501. Camphane.
DB01744. Camphor.
DB01011. Metyrapone.
DB01703. N-(2-Ferrocenylethyl)Maleimide.
DB01826. N-Butyl Isocyanide.
DB03540. Norcamphor.
DB02851. Thiocamphor.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved1 Publication
ChainiPRO_00000522042 – 415Camphor 5-monooxygenaseAdd BLAST414

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
camBP002593EBI-15706256,EBI-15706395

Protein-protein interaction databases

DIPiDIP-29809N.
IntActiP00183. 1 interactor.

Chemistry databases

BindingDBiP00183.

Structurei

Secondary structure

1415
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Turni6 – 8ND:3
Helixi21 – 23ND:3
Beta strandi29 – 31ND:3
Helixi35 – 37ND:3
Helixi39 – 43ND:5
Helixi44 – 47ND:4
Beta strandi48 – 51ND:4
Beta strandi53 – 57ND:5
Helixi59 – 61ND:3
Beta strandi63 – 66ND:4
Helixi69 – 77ND:9
Turni79 – 81ND:3
Beta strandi82 – 86ND:5
Beta strandi88 – 90ND:3
Helixi91 – 96ND:6
Turni100 – 103ND:4
Turni106 – 108ND:3
Helixi109 – 120ND:12
Helixi122 – 143ND:22
Helixi144 – 146ND:3
Beta strandi147 – 150ND:4
Helixi151 – 154ND:4
Turni155 – 157ND:3
Helixi158 – 168ND:11
Helixi172 – 174ND:3
Helixi175 – 186ND:12
Beta strandi190 – 192ND:3
Helixi194 – 214ND:21
Helixi220 – 225ND:6
Beta strandi230 – 233ND:4
Helixi236 – 251ND:16
Helixi253 – 266ND:14
Helixi269 – 277ND:9
Helixi279 – 281ND:3
Helixi282 – 292ND:11
Beta strandi298 – 304ND:7
Beta strandi306 – 308ND:3
Beta strandi311 – 313ND:3
Beta strandi318 – 321ND:4
Helixi323 – 328ND:6
Turni330 – 332ND:3
Beta strandi333 – 335ND:3
Helixi354 – 356ND:3
Helixi361 – 378ND:18
Beta strandi382 – 384ND:3
Beta strandi395 – 397ND:3
Beta strandi399 – 401ND:3
Beta strandi404 – 406ND:3
Helixi409 – 411ND:3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1AKDX-ray1.80A2-415[»]
1C8JX-ray2.10A/B2-415[»]
1CP4X-ray1.90A2-415[»]
1DZ4X-ray1.60A/B2-415[»]
1DZ6X-ray1.90A/B2-415[»]
1DZ8X-ray1.90A/B2-415[»]
1DZ9X-ray1.90A/B2-415[»]
1GEBX-ray2.03A1-415[»]
1GEKX-ray1.70A1-415[»]
1GEMX-ray2.00A1-415[»]
1GJMX-ray2.20A2-415[»]
1IWIX-ray2.00A1-415[»]
1IWJX-ray2.00A1-415[»]
1IWKX-ray2.00A1-415[»]
1J51X-ray2.20A/B/C/D2-415[»]
1K2OX-ray1.65A/B2-415[»]
1LWLX-ray2.20A1-415[»]
1MPWX-ray2.34A/B2-415[»]
1NOOX-ray2.20A2-415[»]
1O76X-ray1.80A/B2-415[»]
1P2YX-ray2.30A2-415[»]
1P7RX-ray2.85A2-415[»]
1PHAX-ray1.63A2-415[»]
1PHBX-ray1.60A2-415[»]
1PHCX-ray1.60A2-415[»]
1PHDX-ray1.60A2-415[»]
1PHEX-ray1.60A2-415[»]
1PHFX-ray1.60A2-415[»]
1PHGX-ray1.60A2-415[»]
1QMQX-ray1.55A2-415[»]
1RE9X-ray1.45A2-415[»]
1RF9X-ray1.80A1-415[»]
1T85X-ray1.80A2-415[»]
1T86X-ray1.90A/B2-415[»]
1T87X-ray1.80A/B2-415[»]
1T88X-ray1.90A/B2-415[»]
1UYUX-ray2.00A/B2-415[»]
1YRCX-ray1.40A2-415[»]
1YRDX-ray1.70A2-415[»]
2A1MX-ray2.10A/B1-415[»]
2A1NX-ray1.90A/B1-415[»]
2A1OX-ray1.55A/B1-415[»]
2CP4X-ray2.10A2-415[»]
2CPPX-ray1.63A2-415[»]
2FE6X-ray1.50A1-415[»]
2FERX-ray1.70A11-415[»]
2FEUX-ray1.70A/B11-415[»]
2FRZX-ray2.10A/B2-415[»]
2GQXX-ray2.10A/B11-415[»]
2GR6X-ray2.30A/B11-415[»]
2H7QX-ray1.50A2-415[»]
2H7RX-ray2.10A2-415[»]
2H7SX-ray2.15A/C2-415[»]
2L8MNMR-A1-415[»]
2LQDNMR-A3-415[»]
2M56NMR-A12-415[»]
2QBLX-ray1.80A1-415[»]
2QBMX-ray1.80A1-415[»]
2QBNX-ray1.75A1-415[»]
2QBOX-ray1.90A1-415[»]
2Z97X-ray1.80A1-415[»]
2ZAWX-ray1.55A1-415[»]
2ZAXX-ray1.60A1-415[»]
2ZUHX-ray1.55A1-415[»]
2ZUIX-ray1.50A1-415[»]
2ZUJX-ray1.60A1-415[»]
2ZWTX-ray1.35A1-415[»]
2ZWUX-ray1.30A1-415[»]
3CP4X-ray2.30A2-415[»]
3CPPX-ray1.90A2-415[»]
3FWFX-ray1.83A/B11-415[»]
3FWGX-ray1.55A/B11-415[»]
3FWIX-ray2.40A11-415[»]
3FWJX-ray1.90A11-415[»]
3L61X-ray1.50A2-415[»]
3L62X-ray1.70A2-415[»]
3L63X-ray1.50A2-415[»]
3OIAX-ray1.65A2-415[»]
3OL5X-ray1.75A2-415[»]
3P6MX-ray2.00A2-415[»]
3P6NX-ray1.70A2-415[»]
3P6OX-ray2.00A2-415[»]
3P6PX-ray1.90A2-415[»]
3P6QX-ray1.95A2-415[»]
3P6RX-ray2.10A2-415[»]
3P6SX-ray2.00A2-415[»]
3P6TX-ray1.90A2-415[»]
3P6UX-ray1.70A2-415[»]
3P6VX-ray2.00A2-415[»]
3P6WX-ray2.10A2-415[»]
3P6XX-ray1.65A2-415[»]
3W9CX-ray2.50A2-415[»]
3WRHX-ray1.62A/E1-415[»]
3WRIX-ray2.90A/B1-415[»]
3WRJX-ray1.85A/E1-415[»]
3WRKX-ray2.61A/D1-415[»]
3WRLX-ray1.65A/E1-415[»]
3WRMX-ray1.95A/F1-415[»]
4CP4X-ray2.10A2-415[»]
4CPPX-ray2.11A2-415[»]
4EK1X-ray1.97A/B2-415[»]
4G3RX-ray2.20A/B2-415[»]
4JWSX-ray2.15A/B1-415[»]
4JWUX-ray2.20A/B1-415[»]
4JX1X-ray2.09A/B/E/F1-415[»]
4KKYX-ray2.00X2-414[»]
4L49X-ray2.13A1-415[»]
4L4AX-ray2.10A1-415[»]
4L4BX-ray2.10A1-415[»]
4L4CX-ray2.20A/B1-415[»]
4L4DX-ray2.10A1-415[»]
4L4EX-ray1.26A1-415[»]
4L4FX-ray1.29A1-415[»]
4L4GX-ray1.55A1-415[»]
5CP4X-ray1.75A2-415[»]
5CPPX-ray2.08A2-415[»]
5GXGX-ray1.70A2-415[»]
5IK1X-ray1.53A10-415[»]
5WK7X-ray1.98A1-415[»]
5WK9X-ray1.98A1-415[»]
6CP4X-ray1.90A2-415[»]
6CPPX-ray1.90A2-415[»]
7CPPX-ray2.00A2-415[»]
8CPPX-ray2.10A2-415[»]
ProteinModelPortaliP00183.
SMRiP00183.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP00183.

Family & Domainsi

Sequence similaritiesi

Belongs to the cytochrome P450 family.IKR:

Phylogenomic databases

KOiK21569.

Family and domain databases

Gene3Di1.10.630.10. 1 hit.
InterProiView protein in InterPro
IPR001128. Cyt_P450.
IPR002397. Cyt_P450_B.
IPR017972. Cyt_P450_CS.
IPR036396. Cyt_P450_sf.
PfamiView protein in Pfam
PF00067. p450. 1 hit.
PRINTSiPR00359. BP450.
PR00385. P450.
SUPFAMiSSF48264. SSF48264. 1 hit.
PROSITEiView protein in PROSITE
PS00086. CYTOCHROME_P450. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P00183-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTTETIQSNA NLAPLPPHVP EHLVFDFDMY NPSNLSAGVQ EAWAVLQESN
60 70 80 90 100
VPDLVWTRCN GGHWIATRGQ LIREAYEDYR HFSSECPFIP REAGEAYDFI
110 120 130 140 150
PTSMDPPEQR QFRALANQVV GMPVVDKLEN RIQELACSLI ESLRPQGQCN
160 170 180 190 200
FTEDYAEPFP IRIFMLLAGL PEEDIPHLKY LTDQMTRPDG SMTFAEAKEA
210 220 230 240 250
LYDYLIPIIE QRRQKPGTDA ISIVANGQVN GRPITSDEAK RMCGLLLVGG
260 270 280 290 300
LDTVVNFLSF SMEFLAKSPE HRQELIERPE RIPAACEELL RRFSLVADGR
310 320 330 340 350
ILTSDYEFHG VQLKKGDQIL LPQMLSGLDE RENACPMHVD FSRQKVSHTT
360 370 380 390 400
FGHGSHLCLG QHLARREIIV TLKEWLTRIP DFSIAPGAQI QHKSGIVSGV
410
QALPLVWDPA TTKAV
Length:415
Mass (Da):46,669
Last modified:January 23, 2007 - v2
Checksum:iE84641B4A65DD2D3
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti56 – 57Missing AA sequence (PubMed:7130171).IKR:2
Sequence conflicti277E → Q AA sequence (PubMed:7130171).IKR:1
Sequence conflicti362H → S AA sequence (PubMed:7130171).IKR:1
Sequence conflicti408D → N AA sequence (PubMed:7130171).IKR:1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M12546 Genomic DNA. Translation: AAA25760.1.
D00528 Genomic DNA. Translation: BAA00412.1.
PIRiA25660. O4PSCP.

Genome annotation databases

KEGGiag:AAA25760.

Similar proteinsi

Entry informationi

Entry nameiCPXA_PSEPU
AccessioniPrimary (citable) accession number: P00183
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: January 23, 2007
Last modified: October 25, 2017
This is version 149 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Direct protein sequencing

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families