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Protein

Cytochrome P450 2C3

Gene

CYP2C3

Organism
Oryctolagus cuniculus (Rabbit)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Cytochromes P450 are a group of heme-thiolate monooxygenases. In liver microsomes, this enzyme is involved in an NADPH-dependent electron transport pathway. It oxidizes a variety of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics.

Catalytic activityi

RH + reduced flavoprotein + O2 = ROH + oxidized flavoprotein + H2O.

Cofactori

hemeBy similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi434 – 4341Iron (heme axial ligand)

GO - Molecular functioni

  1. aromatase activity Source: UniProtKB-EC
  2. heme binding Source: InterPro
  3. iron ion binding Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Monooxygenase, Oxidoreductase

Keywords - Ligandi

Heme, Iron, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Cytochrome P450 2C3 (EC:1.14.14.1)
Alternative name(s):
CYPIIC3
Cytochrome P450 PBc3
Cytochrome P450 form 3B
Gene namesi
Name:CYP2C3
OrganismiOryctolagus cuniculus (Rabbit)
Taxonomic identifieri9986 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresLagomorphaLeporidaeOryctolagus
ProteomesiUP000001811 Componenti: Unplaced

Subcellular locationi

GO - Cellular componenti

  1. endoplasmic reticulum membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane, Microsome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 489489Cytochrome P450 2C3PRO_0000051694Add
BLAST

Expressioni

Inductioni

Constitutively expressed.

Interactioni

Protein-protein interaction databases

STRINGi9986.ENSOCUP00000016913.

Structurei

3D structure databases

ProteinModelPortaliP00182.
SMRiP00182. Positions 30-489.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the cytochrome P450 family.Curated

Phylogenomic databases

eggNOGiCOG2124.
HOGENOMiHOG000036992.
HOVERGENiHBG015789.
InParanoidiP00182.
KOiK07413.

Family and domain databases

Gene3Di1.10.630.10. 1 hit.
InterProiIPR001128. Cyt_P450.
IPR017972. Cyt_P450_CS.
IPR002401. Cyt_P450_E_grp-I.
[Graphical view]
PfamiPF00067. p450. 1 hit.
[Graphical view]
PRINTSiPR00463. EP450I.
PR00385. P450.
SUPFAMiSSF48264. SSF48264. 1 hit.
PROSITEiPS00086. CYTOCHROME_P450. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P00182-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDLLIILGIC LSCVVLLSLW KKTHGKGKLP PGPTPLPVVG NLLQLETKDI
60 70 80 90 100
NKSLSMLAKE YGSIFTLYFG MKPAVVLYGY EGVIEALIDR GEEFSGRGIF
110 120 130 140 150
PVFDRVTKGL GIVFSSGEKW KETRRFSLTV LRNLGMGKKT IEERIQEEAL
160 170 180 190 200
CLIQALRKTN ASPCDPTFLL FCVPCNVICS VIFQNRFDYD DEKFKTLIKY
210 220 230 240 250
FHENFELLGT PWIQLYNIFP ILHYLPGSHR QLFKNIDGQI KFILEKVQEH
260 270 280 290 300
QESLDSNNPR DFVDHFLIKM EKEKHKKQSE FTMDNLITTI WDVFSAGTDT
310 320 330 340 350
TSNTLKFALL LLLKHPEITA KVQEEIEHVI GRHRSPCMQD RTRMPYTDAV
360 370 380 390 400
MHEIQRYVDL VPTSLPHAVT QDIEFNGYLI PKGTDIIPSL TSVLYDDKEF
410 420 430 440 450
PNPEKFDPGH FLDESGNFKK SDYFMPFSAG KRACVGEGLA RMELFLLLTT
460 470 480
ILQHFTLKPL VDPKDIDPTP VENGFVSVPP SYELCFVPV
Length:489
Mass (Da):55,794
Last modified:December 15, 1998 - v2
Checksum:i860D36E42D554C75
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti49 – 491D → N in AAA31175 (PubMed:2340269).Curated
Sequence conflicti49 – 491D → N in BAA05139 (Ref. 4) Curated
Sequence conflicti82 – 843GVI → TVK in AAA31175 (PubMed:2340269).Curated
Sequence conflicti82 – 843GVI → TVK in BAA05139 (Ref. 4) Curated
Sequence conflicti89 – 891D → Y AA sequence (PubMed:3988762).Curated
Sequence conflicti178 – 1781I → M in BAA05139 (Ref. 4) Curated
Sequence conflicti222 – 2221L → LG AA sequence (PubMed:3988762).Curated
Sequence conflicti256 – 2561S → L in AAA31175 (PubMed:2340269).Curated
Sequence conflicti338 – 3381M → S AA sequence (PubMed:3988762).Curated
Sequence conflicti342 – 3421T → S AA sequence (PubMed:3988762).Curated
Sequence conflicti364 – 3641S → T in BAA05139 (Ref. 4) Curated
Sequence conflicti429 – 4291A → T AA sequence (PubMed:3988762).Curated
Sequence conflicti471 – 4711V → L in BAA05139 (Ref. 4) Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
K01523 mRNA. Translation: AAA31212.1.
M31254
, M31245, M31247, M31248, M31249, M31250, M31251, M31252, M31253 Genomic DNA. Translation: AAA31175.1.
D26152 mRNA. Translation: BAA05139.1.
PIRiA00183. O4RBP3.
RefSeqiNP_001164736.1. NM_001171265.1.
UniGeneiOcu.1852.

Genome annotation databases

GeneIDi100328933.
KEGGiocu:100328933.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
K01523 mRNA. Translation: AAA31212.1.
M31254
, M31245, M31247, M31248, M31249, M31250, M31251, M31252, M31253 Genomic DNA. Translation: AAA31175.1.
D26152 mRNA. Translation: BAA05139.1.
PIRiA00183. O4RBP3.
RefSeqiNP_001164736.1. NM_001171265.1.
UniGeneiOcu.1852.

3D structure databases

ProteinModelPortaliP00182.
SMRiP00182. Positions 30-489.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9986.ENSOCUP00000016913.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi100328933.
KEGGiocu:100328933.

Organism-specific databases

CTDi100328933.

Phylogenomic databases

eggNOGiCOG2124.
HOGENOMiHOG000036992.
HOVERGENiHBG015789.
InParanoidiP00182.
KOiK07413.

Family and domain databases

Gene3Di1.10.630.10. 1 hit.
InterProiIPR001128. Cyt_P450.
IPR017972. Cyt_P450_CS.
IPR002401. Cyt_P450_E_grp-I.
[Graphical view]
PfamiPF00067. p450. 1 hit.
[Graphical view]
PRINTSiPR00463. EP450I.
PR00385. P450.
SUPFAMiSSF48264. SSF48264. 1 hit.
PROSITEiPS00086. CYTOCHROME_P450. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "The complete amino acid sequence of a constitutive form of liver microsomal cytochrome P-450."
    Ozols J., Heinemann F.S., Johnson E.F.
    J. Biol. Chem. 260:5427-5434(1984) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE.
  2. "Isolation and sequence analysis of three cloned cDNAs for rabbit liver proteins that are related to rabbit cytochrome P-450 (form 2), the major phenobarbital-inducible form."
    Leighton J.K., Debrunner-Vossbrinck B.A., Kemper B.
    Biochemistry 23:204-210(1983) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  3. "Structure of the rabbit cytochrome P450IIC3 gene, a constitutive member of the P450IIC subfamily."
    Chan G.
    Biochemistry 29:3743-3750(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  4. "A member of cytochrome P450 2C family which is identical to rabbit P450pc3."
    Noshiro M., Ishida H., Okuda K.
    Submitted (DEC-1993) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: New Zealand white.
    Tissue: Liver.

Entry informationi

Entry nameiCP2C3_RABIT
AccessioniPrimary (citable) accession number: P00182
Secondary accession number(s): Q29509, Q29525
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: December 15, 1998
Last modified: February 4, 2015
This is version 105 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.