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P00179

- CP2C5_RABIT

UniProt

P00179 - CP2C5_RABIT

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Protein
Cytochrome P450 2C5
Gene
CYP2C5
Organism
Oryctolagus cuniculus (Rabbit)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at protein leveli

Functioni

Cytochromes P450 are a group of heme-thiolate monooxygenases. In liver microsomes, this enzyme is involved in an NADPH-dependent electron transport pathway. It oxidizes a variety of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics.

Catalytic activityi

RH + reduced flavoprotein + O2 = ROH + oxidized flavoprotein + H2O.

Cofactori

Heme group.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi432 – 4321Iron (heme axial ligand)

GO - Molecular functioni

  1. aromatase activity Source: UniProtKB-EC
  2. heme binding Source: InterPro
  3. iron ion binding Source: InterPro
Complete GO annotation...

GO - Biological processi

    Complete GO annotation...

    Keywords - Molecular functioni

    Monooxygenase, Oxidoreductase

    Keywords - Ligandi

    Heme, Iron, Metal-binding

    Enzyme and pathway databases

    SABIO-RKP00179.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Cytochrome P450 2C5 (EC:1.14.14.1)
    Alternative name(s):
    CYPIIC5
    Cytochrome P450 1
    Cytochrome P450IIC5
    Progesterone 21-hydroxylase
    Gene namesi
    Name:CYP2C5
    OrganismiOryctolagus cuniculus (Rabbit)
    Taxonomic identifieri9986 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresLagomorphaLeporidaeOryctolagus
    ProteomesiUP000001811: Chromosome 18

    Subcellular locationi

    GO - Cellular componenti

    1. endoplasmic reticulum membrane Source: UniProtKB-SubCell
    Complete GO annotation...

    Keywords - Cellular componenti

    Endoplasmic reticulum, Membrane, Microsome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 487487Cytochrome P450 2C5
    PRO_0000051696Add
    BLAST

    Expressioni

    Inductioni

    P450 can be induced to high levels in liver and other tissues by various foreign compounds, including drugs, pesticides, and carcinogens.

    Interactioni

    Protein-protein interaction databases

    STRINGi9986.ENSOCUP00000020892.

    Structurei

    Secondary structure

    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Turni37 – 393
    Helixi42 – 443
    Helixi50 – 6112
    Beta strandi63 – 7715
    Helixi80 – 878
    Turni88 – 947
    Beta strandi95 – 973
    Helixi103 – 1075
    Beta strandi111 – 1144
    Helixi117 – 13014
    Turni133 – 1364
    Beta strandi137 – 1393
    Helixi141 – 15717
    Turni158 – 1614
    Helixi167 – 18317
    Helixi192 – 20716
    Helixi214 – 2174
    Helixi220 – 2256
    Helixi227 – 25428
    Helixi263 – 27210
    Beta strandi273 – 2753
    Helixi281 – 31232
    Helixi314 – 32714
    Beta strandi330 – 3323
    Helixi336 – 3416
    Helixi343 – 35614
    Beta strandi371 – 3733
    Beta strandi376 – 3783
    Beta strandi383 – 3864
    Helixi388 – 3925
    Turni395 – 3973
    Beta strandi398 – 4003
    Helixi406 – 4094
    Beta strandi412 – 4154
    Helixi428 – 4303
    Helixi435 – 45218
    Beta strandi453 – 4597
    Helixi461 – 4633
    Beta strandi472 – 4765
    Beta strandi482 – 4865

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1DT6X-ray3.00A22-487[»]
    1N6BX-ray2.30A19-487[»]
    1NR6X-ray2.10A19-487[»]
    ProteinModelPortaliP00179.
    SMRiP00179. Positions 27-487.

    Miscellaneous databases

    EvolutionaryTraceiP00179.

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the cytochrome P450 family.

    Phylogenomic databases

    eggNOGiCOG2124.
    GeneTreeiENSGT00680000099783.
    HOGENOMiHOG000036992.
    HOVERGENiHBG015789.
    OMAiHDNTEFP.
    OrthoDBiEOG7RBZ85.
    TreeFamiTF352043.

    Family and domain databases

    Gene3Di1.10.630.10. 1 hit.
    InterProiIPR001128. Cyt_P450.
    IPR017972. Cyt_P450_CS.
    IPR002401. Cyt_P450_E_grp-I.
    [Graphical view]
    PfamiPF00067. p450. 1 hit.
    [Graphical view]
    PRINTSiPR00463. EP450I.
    PR00385. P450.
    SUPFAMiSSF48264. SSF48264. 1 hit.
    PROSITEiPS00086. CYTOCHROME_P450. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P00179-1 [UniParc]FASTAAdd to Basket

    « Hide

    MDPVVVLVLG LCCLLLLSIW KQNSGRGKLP PGPTPFPIIG NILQIDAKDI    50
    SKSLTKFSEC YGPVFTVYLG MKPTVVLHGY EAVKEALVDL GEEFAGRGSV 100
    PILEKVSKGL GIAFSNAKTW KEMRRFSLMT LRNFGMGKRS IEDRIQEEAR 150
    CLVEELRKTN ASPCDPTFIL GCAPCNVICS VIFHNRFDYK DEEFLKLMES 200
    LNENVRILSS PWLQVYNNFP ALLDYFPGIH KTLLKNADYI KNFIMEKVKE 250
    HQKLLDVNNP RDFIDCFLIK MEQENNLEFT LESLVIAVSD LFGAGTETTS 300
    TTLRYSLLLL LKHPEVAARV QEEIERVIGR HRSPCMQDRS RMPYTDAVIH 350
    EIQRFIDLLP TNLPHAVTRD VRFRNYFIPK GTDIITSLTS VLHDEKAFPN 400
    PKVFDPGHFL DESGNFKKSD YFMPFSAGKR MCVGEGLARM ELFLFLTSIL 450
    QNFKLQSLVE PKDLDITAVV NGFVSVPPSY QLCFIPI 487
    Length:487
    Mass (Da):55,275
    Last modified:September 5, 2006 - v2
    Checksum:iED67B2E255DF5EA0
    GO

    Sequence conflict

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti97 – 971R → T in AAA31209. 1 Publication
    Sequence conflicti252 – 2521Q → E in AAA31209. 1 Publication

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M11299 mRNA. Translation: AAA31209.1.
    M55664 mRNA. Translation: AAA63461.1.
    PIRiA00180. O4RBP4.
    RefSeqiNP_001164397.1. NM_001170926.1.
    UniGeneiOcu.1857.

    Genome annotation databases

    EnsembliENSOCUT00000021366; ENSOCUP00000020892; ENSOCUG00000021501.
    GeneIDi100328549.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M11299 mRNA. Translation: AAA31209.1 .
    M55664 mRNA. Translation: AAA63461.1 .
    PIRi A00180. O4RBP4.
    RefSeqi NP_001164397.1. NM_001170926.1.
    UniGenei Ocu.1857.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1DT6 X-ray 3.00 A 22-487 [» ]
    1N6B X-ray 2.30 A 19-487 [» ]
    1NR6 X-ray 2.10 A 19-487 [» ]
    ProteinModelPortali P00179.
    SMRi P00179. Positions 27-487.
    ModBasei Search...

    Protein-protein interaction databases

    STRINGi 9986.ENSOCUP00000020892.

    Chemistry

    ChEMBLi CHEMBL1907985.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSOCUT00000021366 ; ENSOCUP00000020892 ; ENSOCUG00000021501 .
    GeneIDi 100328549.

    Organism-specific databases

    CTDi 100328549.

    Phylogenomic databases

    eggNOGi COG2124.
    GeneTreei ENSGT00680000099783.
    HOGENOMi HOG000036992.
    HOVERGENi HBG015789.
    OMAi HDNTEFP.
    OrthoDBi EOG7RBZ85.
    TreeFami TF352043.

    Enzyme and pathway databases

    SABIO-RK P00179.

    Miscellaneous databases

    EvolutionaryTracei P00179.

    Family and domain databases

    Gene3Di 1.10.630.10. 1 hit.
    InterProi IPR001128. Cyt_P450.
    IPR017972. Cyt_P450_CS.
    IPR002401. Cyt_P450_E_grp-I.
    [Graphical view ]
    Pfami PF00067. p450. 1 hit.
    [Graphical view ]
    PRINTSi PR00463. EP450I.
    PR00385. P450.
    SUPFAMi SSF48264. SSF48264. 1 hit.
    PROSITEi PS00086. CYTOCHROME_P450. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Multiple gene-like sequences related to the rabbit hepatic progesterone 21-hydroxylase cytochrome P-450 1."
      Tukey R.H., Okino S., Barnes H.J., Griffin K.J., Johnson E.F.
      J. Biol. Chem. 260:13347-13354(1985) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    2. Tukey R.H.
      Submitted (FEB-1986) to the EMBL/GenBank/DDBJ databases
      Cited for: SEQUENCE REVISION TO 252.
    3. "Characterization of the CYP2C5 gene in 21L III/J rabbits. Allelic variation affects the expression of P450IIC5."
      Pendurthi U.R., Lamb J.G., Nguyen N., Johnson E.F., Tukey R.H.
      J. Biol. Chem. 265:14662-14668(1990) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: III/J.
      Tissue: Liver.
    4. "Mammalian microsomal cytochrome P450 monooxygenase: structural adaptations for membrane binding and functional diversity."
      Williams P.A., Cosme J., Sridhar V., Johnson E.F., McRee D.E.
      Mol. Cell 5:121-131(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (3.0 ANGSTROMS).
    5. "Structure of a substrate complex of mammalian cytochrome P450 2C5 at 2.3 A resolution: evidence for multiple substrate binding modes."
      Wester M.R., Johnson E.F., Marques-Soares C., Dansette P.M., Mansuy D., Stout C.D.
      Biochemistry 42:6370-6379(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.3 ANGSTROMS) IN COMPLEX WITH SUBSTRATE ANALOG.

    Entry informationi

    Entry nameiCP2C5_RABIT
    AccessioniPrimary (citable) accession number: P00179
    Secondary accession number(s): Q29511
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 21, 1986
    Last sequence update: September 5, 2006
    Last modified: July 9, 2014
    This is version 111 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    This protein differs from other forms of cytochrome P450 in that it catalyzes the 21-hydroxylation of progesterone, resulting in the formation of deoxycorticosterone.

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3

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