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P00179 (CP2C5_RABIT) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 109. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Cytochrome P450 2C5

EC=1.14.14.1
Alternative name(s):
CYPIIC5
Cytochrome P450 1
Cytochrome P450IIC5
Progesterone 21-hydroxylase
Gene names
Name:CYP2C5
OrganismOryctolagus cuniculus (Rabbit) [Reference proteome]
Taxonomic identifier9986 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresLagomorphaLeporidaeOryctolagus

Protein attributes

Sequence length487 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Cytochromes P450 are a group of heme-thiolate monooxygenases. In liver microsomes, this enzyme is involved in an NADPH-dependent electron transport pathway. It oxidizes a variety of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics.

Catalytic activity

RH + reduced flavoprotein + O2 = ROH + oxidized flavoprotein + H2O.

Cofactor

Heme group.

Subcellular location

Endoplasmic reticulum membrane By similarity. Microsome membrane By similarity.

Induction

P450 can be induced to high levels in liver and other tissues by various foreign compounds, including drugs, pesticides, and carcinogens.

Miscellaneous

This protein differs from other forms of cytochrome P450 in that it catalyzes the 21-hydroxylation of progesterone, resulting in the formation of deoxycorticosterone.

Sequence similarities

Belongs to the cytochrome P450 family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 487487Cytochrome P450 2C5
PRO_0000051696

Sites

Metal binding4321Iron (heme axial ligand)

Experimental info

Sequence conflict971R → T in AAA31209. Ref.1
Sequence conflict2521Q → E in AAA31209. Ref.1

Secondary structure

.......................................................................... 487
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P00179 [UniParc].

Last modified September 5, 2006. Version 2.
Checksum: ED67B2E255DF5EA0

FASTA48755,275
        10         20         30         40         50         60 
MDPVVVLVLG LCCLLLLSIW KQNSGRGKLP PGPTPFPIIG NILQIDAKDI SKSLTKFSEC 

        70         80         90        100        110        120 
YGPVFTVYLG MKPTVVLHGY EAVKEALVDL GEEFAGRGSV PILEKVSKGL GIAFSNAKTW 

       130        140        150        160        170        180 
KEMRRFSLMT LRNFGMGKRS IEDRIQEEAR CLVEELRKTN ASPCDPTFIL GCAPCNVICS 

       190        200        210        220        230        240 
VIFHNRFDYK DEEFLKLMES LNENVRILSS PWLQVYNNFP ALLDYFPGIH KTLLKNADYI 

       250        260        270        280        290        300 
KNFIMEKVKE HQKLLDVNNP RDFIDCFLIK MEQENNLEFT LESLVIAVSD LFGAGTETTS 

       310        320        330        340        350        360 
TTLRYSLLLL LKHPEVAARV QEEIERVIGR HRSPCMQDRS RMPYTDAVIH EIQRFIDLLP 

       370        380        390        400        410        420 
TNLPHAVTRD VRFRNYFIPK GTDIITSLTS VLHDEKAFPN PKVFDPGHFL DESGNFKKSD 

       430        440        450        460        470        480 
YFMPFSAGKR MCVGEGLARM ELFLFLTSIL QNFKLQSLVE PKDLDITAVV NGFVSVPPSY 


QLCFIPI 

« Hide

References

[1]"Multiple gene-like sequences related to the rabbit hepatic progesterone 21-hydroxylase cytochrome P-450 1."
Tukey R.H., Okino S., Barnes H.J., Griffin K.J., Johnson E.F.
J. Biol. Chem. 260:13347-13354(1985) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]Tukey R.H.
Submitted (FEB-1986) to the EMBL/GenBank/DDBJ databases
Cited for: SEQUENCE REVISION TO 252.
[3]"Characterization of the CYP2C5 gene in 21L III/J rabbits. Allelic variation affects the expression of P450IIC5."
Pendurthi U.R., Lamb J.G., Nguyen N., Johnson E.F., Tukey R.H.
J. Biol. Chem. 265:14662-14668(1990) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: III/J.
Tissue: Liver.
[4]"Mammalian microsomal cytochrome P450 monooxygenase: structural adaptations for membrane binding and functional diversity."
Williams P.A., Cosme J., Sridhar V., Johnson E.F., McRee D.E.
Mol. Cell 5:121-131(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (3.0 ANGSTROMS).
[5]"Structure of a substrate complex of mammalian cytochrome P450 2C5 at 2.3 A resolution: evidence for multiple substrate binding modes."
Wester M.R., Johnson E.F., Marques-Soares C., Dansette P.M., Mansuy D., Stout C.D.
Biochemistry 42:6370-6379(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (2.3 ANGSTROMS) IN COMPLEX WITH SUBSTRATE ANALOG.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M11299 mRNA. Translation: AAA31209.1.
M55664 mRNA. Translation: AAA63461.1.
PIRO4RBP4. A00180.
RefSeqNP_001164397.1. NM_001170926.1.
UniGeneOcu.1857.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1DT6X-ray3.00A27-487[»]
1N6BX-ray2.30A27-487[»]
1NR6X-ray2.10A27-487[»]
ProteinModelPortalP00179.
SMRP00179. Positions 27-487.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING9986.ENSOCUP00000020892.

Chemistry

ChEMBLCHEMBL1907985.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSOCUT00000021366; ENSOCUP00000020892; ENSOCUG00000021501.
GeneID100328549.

Organism-specific databases

CTD100328549.

Phylogenomic databases

eggNOGCOG2124.
GeneTreeENSGT00680000099783.
HOGENOMHOG000036992.
HOVERGENHBG015789.
OMAKHTNITA.
OrthoDBEOG7RBZ85.
TreeFamTF352043.

Enzyme and pathway databases

SABIO-RKP00179.

Family and domain databases

Gene3D1.10.630.10. 1 hit.
InterProIPR001128. Cyt_P450.
IPR017972. Cyt_P450_CS.
IPR002401. Cyt_P450_E_grp-I.
[Graphical view]
PfamPF00067. p450. 1 hit.
[Graphical view]
PRINTSPR00463. EP450I.
PR00385. P450.
SUPFAMSSF48264. SSF48264. 1 hit.
PROSITEPS00086. CYTOCHROME_P450. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceP00179.

Entry information

Entry nameCP2C5_RABIT
AccessionPrimary (citable) accession number: P00179
Secondary accession number(s): Q29511
Entry history
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: September 5, 2006
Last modified: February 19, 2014
This is version 109 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references