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P00178

- CP2B4_RABIT

UniProt

P00178 - CP2B4_RABIT

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Protein

Cytochrome P450 2B4

Gene
CYP2B4
Organism
Oryctolagus cuniculus (Rabbit)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Cytochromes P450 are a group of heme-thiolate monooxygenases. In liver microsomes, this enzyme is involved in an NADPH-dependent electron transport pathway. It oxidizes a variety of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics. In the epoxidation of arachidonic acid it has a unique preference for the 5,6-olefin.

Catalytic activityi

RH + reduced flavoprotein + O2 = ROH + oxidized flavoprotein + H2O.

Cofactori

Heme group.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi436 – 4361Iron (heme axial ligand)

GO - Molecular functioni

  1. aromatase activity Source: UniProtKB-EC
  2. heme binding Source: InterPro
  3. iron ion binding Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Monooxygenase, Oxidoreductase

Keywords - Ligandi

Heme, Iron, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Cytochrome P450 2B4 (EC:1.14.14.1)
Alternative name(s):
CYPIIB4
Cytochrome P450 isozyme 2
Short name:
Cytochrome P450 LM2
Cytochrome P450 type B0
Cytochrome P450 type B1
Gene namesi
Name:CYP2B4
OrganismiOryctolagus cuniculus (Rabbit)
Taxonomic identifieri9986 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresLagomorphaLeporidaeOryctolagus
ProteomesiUP000001811: Unplaced

Subcellular locationi

GO - Cellular componenti

  1. endoplasmic reticulum membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane, Microsome

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi436 – 4361C → S: Conversion into an NADPH oxidase with negligible monooxygenase activity. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 491491Cytochrome P450 2B4PRO_0000051681Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei128 – 1281Phosphoserine; by PKA By similarity

Keywords - PTMi

Phosphoprotein

Expressioni

Inductioni

By phenobarbital.

Structurei

Secondary structure

1
491
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Turni38 – 403
Helixi43 – 453
Helixi51 – 6212
Beta strandi64 – 707
Beta strandi73 – 786
Helixi81 – 888
Turni89 – 957
Helixi101 – 1044
Helixi105 – 1084
Helixi112 – 1154
Helixi118 – 13417
Turni135 – 1384
Helixi139 – 15921
Turni160 – 1623
Beta strandi165 – 1673
Helixi168 – 18417
Helixi193 – 20513
Turni206 – 2083
Helixi213 – 2197
Helixi221 – 2255
Helixi231 – 25323
Beta strandi258 – 2603
Helixi264 – 27512
Helixi282 – 31635
Helixi318 – 33114
Beta strandi334 – 3363
Helixi340 – 3456
Helixi347 – 36014
Beta strandi362 – 3654
Beta strandi375 – 3773
Beta strandi380 – 3823
Beta strandi387 – 3904
Helixi392 – 3965
Turni399 – 4013
Beta strandi402 – 4043
Helixi410 – 4134
Beta strandi418 – 4203
Helixi432 – 4343
Helixi439 – 45618
Beta strandi457 – 4604
Helixi465 – 4673
Beta strandi473 – 4808
Beta strandi486 – 4905

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1PO5X-ray1.60A25-491[»]
1SUOX-ray1.90A25-491[»]
2BDMX-ray2.30A25-491[»]
2Q6NX-ray3.20A/B/C/D/E/F/G25-491[»]
3G5NX-ray2.50A/B/C/D25-491[»]
3G93X-ray3.20A/B/C/D25-491[»]
3KW4X-ray2.67A25-491[»]
3ME6X-ray3.10A/B/C/D25-491[»]
3MVRX-ray1.76A/B25-491[»]
3R1AX-ray3.50A/B/C/D/E/F/G/H25-491[»]
3R1BX-ray3.00A/B/C/D25-491[»]
3TK3X-ray2.80A/B/C/D25-491[»]
3TMZX-ray2.25A25-491[»]
3UASX-ray2.94A25-491[»]
4H1NX-ray2.99A25-491[»]
4JLTX-ray2.14A25-491[»]
ProteinModelPortaliP00178.
SMRiP00178. Positions 28-491.

Miscellaneous databases

EvolutionaryTraceiP00178.

Family & Domainsi

Sequence similaritiesi

Belongs to the cytochrome P450 family.

Phylogenomic databases

eggNOGiCOG2124.
HOGENOMiHOG000036992.
HOVERGENiHBG015789.

Family and domain databases

Gene3Di1.10.630.10. 1 hit.
InterProiIPR001128. Cyt_P450.
IPR017972. Cyt_P450_CS.
IPR002401. Cyt_P450_E_grp-I.
IPR008068. Cyt_P450_E_grp-I_CYP2B-like.
[Graphical view]
PfamiPF00067. p450. 1 hit.
[Graphical view]
PRINTSiPR00463. EP450I.
PR01685. EP450ICYP2B.
PR00385. P450.
SUPFAMiSSF48264. SSF48264. 1 hit.
PROSITEiPS00086. CYTOCHROME_P450. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P00178-1 [UniParc]FASTAAdd to Basket

« Hide

MEFSLLLLLA FLAGLLLLLF RGHPKAHGRL PPGPSPLPVL GNLLQMDRKG    50
LLRSFLRLRE KYGDVFTVYL GSRPVVVLCG TDAIREALVD QAEAFSGRGK 100
IAVVDPIFQG YGVIFANGER WRALRRFSLA TMRDFGMGKR SVEERIQEEA 150
RCLVEELRKS KGALLDNTLL FHSITSNIIC SIVFGKRFDY KDPVFLRLLD 200
LFFQSFSLIS SFSSQVFELF PGFLKHFPGT HRQIYRNLQE INTFIGQSVE 250
KHRATLDPSN PRDFIDVYLL RMEKDKSDPS SEFHHQNLIL TVLSLFFAGT 300
ETTSTTLRYG FLLMLKYPHV TERVQKEIEQ VIGSHRPPAL DDRAKMPYTD 350
AVIHEIQRLG DLIPFGVPHT VTKDTQFRGY VIPKNTEVFP VLSSALHDPR 400
YFETPNTFNP GHFLDANGAL KRNEGFMPFS LGKRICLGEG IARTELFLFF 450
TTILQNFSIA SPVPPEDIDL TPRESGVGNV PPSYQIRFLA R 491
Length:491
Mass (Da):55,713
Last modified:July 21, 1986 - v1
Checksum:i0DB943C6CDCF690B
GO

Polymorphismi

Types B0 and B1 are probably allelic variants.

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti39 – 391V → I in B1.
Natural varianti174 – 1741I → V in B1.
Natural varianti290 – 2901L → I in B1.
Natural varianti314 – 3141M → L in B1.
Natural varianti420 – 4201L → M in B1.

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti91 – 911Q → E AA sequence 1 Publication
Sequence conflicti95 – 962FS → SF AA sequence 1 Publication
Sequence conflicti99 – 1002Missing AA sequence 1 Publication
Sequence conflicti135 – 1362FG → GY AA sequence 1 Publication
Sequence conflicti193 – 1931P → K AA sequence 1 Publication
Sequence conflicti221 – 2211P → S AA sequence 1 Publication
Sequence conflicti303 – 3031T → A AA sequence 1 Publication
Sequence conflicti461 – 4655SPVPP → GNLSL AA sequence 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M20856 mRNA. Translation: AAA65840.1.
M20857 mRNA. Translation: AAA31224.1.
PIRiA00179. O4RBPC.
S31277.
RefSeqiNP_001164602.1. NM_001171131.1.
UniGeneiOcu.1858.
Ocu.7479.

Genome annotation databases

GeneIDi100328948.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M20856 mRNA. Translation: AAA65840.1 .
M20857 mRNA. Translation: AAA31224.1 .
PIRi A00179. O4RBPC.
S31277.
RefSeqi NP_001164602.1. NM_001171131.1.
UniGenei Ocu.1858.
Ocu.7479.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1PO5 X-ray 1.60 A 25-491 [» ]
1SUO X-ray 1.90 A 25-491 [» ]
2BDM X-ray 2.30 A 25-491 [» ]
2Q6N X-ray 3.20 A/B/C/D/E/F/G 25-491 [» ]
3G5N X-ray 2.50 A/B/C/D 25-491 [» ]
3G93 X-ray 3.20 A/B/C/D 25-491 [» ]
3KW4 X-ray 2.67 A 25-491 [» ]
3ME6 X-ray 3.10 A/B/C/D 25-491 [» ]
3MVR X-ray 1.76 A/B 25-491 [» ]
3R1A X-ray 3.50 A/B/C/D/E/F/G/H 25-491 [» ]
3R1B X-ray 3.00 A/B/C/D 25-491 [» ]
3TK3 X-ray 2.80 A/B/C/D 25-491 [» ]
3TMZ X-ray 2.25 A 25-491 [» ]
3UAS X-ray 2.94 A 25-491 [» ]
4H1N X-ray 2.99 A 25-491 [» ]
4JLT X-ray 2.14 A 25-491 [» ]
ProteinModelPortali P00178.
SMRi P00178. Positions 28-491.
ModBasei Search...
MobiDBi Search...

Chemistry

ChEMBLi CHEMBL1743542.
DrugBanki DB04794. Bifonazole.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 100328948.

Phylogenomic databases

eggNOGi COG2124.
HOGENOMi HOG000036992.
HOVERGENi HBG015789.

Miscellaneous databases

EvolutionaryTracei P00178.

Family and domain databases

Gene3Di 1.10.630.10. 1 hit.
InterProi IPR001128. Cyt_P450.
IPR017972. Cyt_P450_CS.
IPR002401. Cyt_P450_E_grp-I.
IPR008068. Cyt_P450_E_grp-I_CYP2B-like.
[Graphical view ]
Pfami PF00067. p450. 1 hit.
[Graphical view ]
PRINTSi PR00463. EP450I.
PR01685. EP450ICYP2B.
PR00385. P450.
SUPFAMi SSF48264. SSF48264. 1 hit.
PROSITEi PS00086. CYTOCHROME_P450. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Primary structures of multiple forms of cytochrome P-450 isozyme 2 derived from rabbit pulmonary and hepatic cDNAs."
    Gasser R., Negishi M., Philpot R.M.
    Mol. Pharmacol. 33:22-30(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "Complete amino acid sequence and predicted membrane topology of phenobarbital-induced cytochrome P-450 (isozyme 2) from rabbit liver microsomes."
    Tarr G.E., Black S.D., Fujita V.S., Coon M.J.
    Proc. Natl. Acad. Sci. U.S.A. 80:6552-6556(1983) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE.
  3. "The complete amino acid sequence of rabbit phenobarbital-induced liver microsomal cytochrome P-450."
    Heinemann F.S., Ozols J.
    J. Biol. Chem. 258:4195-4201(1983) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE.
  4. "Cytochrome P-450 isozymes 2 and 5 in rabbit lung and liver. Comparisons of structure and inducibility."
    Parandoosh Z., Fujita V.S., Coon M.J., Philpot R.M.
    Drug Metab. Dispos. 15:59-67(1987) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 1-24.
  5. "Microheterogeneity in the major phenobarbital-inducible forms of rabbit liver microsomal cytochrome P-450 as revealed by nucleotide sequencing of cloned cDNAs."
    Komori M., Imai Y., Tsunasawa S., Sato R.
    Biochemistry 27:73-80(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 301-491 (B1).
  6. "Replacement of active-site cysteine-436 by serine converts cytochrome P450 2B4 into an NADPH oxidase with negligible monooxygenase activity."
    Vatsis K.P., Peng H.-M., Coon M.J.
    J. Inorg. Biochem. 91:542-553(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: MUTAGENESIS OF CYS-436.
  7. Cited for: X-RAY CRYSTALLOGRAPHY (1.6 ANGSTROMS).

Entry informationi

Entry nameiCP2B4_RABIT
AccessioniPrimary (citable) accession number: P00178
Secondary accession number(s): P00177
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: July 21, 1986
Last modified: July 9, 2014
This is version 127 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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