Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

P00178

- CP2B4_RABIT

UniProt

P00178 - CP2B4_RABIT

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein

Cytochrome P450 2B4

Gene

CYP2B4

Organism
Oryctolagus cuniculus (Rabbit)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Cytochromes P450 are a group of heme-thiolate monooxygenases. In liver microsomes, this enzyme is involved in an NADPH-dependent electron transport pathway. It oxidizes a variety of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics. In the epoxidation of arachidonic acid it has a unique preference for the 5,6-olefin.

Catalytic activityi

RH + reduced flavoprotein + O2 = ROH + oxidized flavoprotein + H2O.

Cofactori

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi436 – 4361Iron (heme axial ligand)

GO - Molecular functioni

  1. aromatase activity Source: UniProtKB-EC
  2. heme binding Source: InterPro
  3. iron ion binding Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Monooxygenase, Oxidoreductase

Keywords - Ligandi

Heme, Iron, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Cytochrome P450 2B4 (EC:1.14.14.1)
Alternative name(s):
CYPIIB4
Cytochrome P450 isozyme 2
Short name:
Cytochrome P450 LM2
Cytochrome P450 type B0
Cytochrome P450 type B1
Gene namesi
Name:CYP2B4
OrganismiOryctolagus cuniculus (Rabbit)
Taxonomic identifieri9986 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresLagomorphaLeporidaeOryctolagus
ProteomesiUP000001811: Unplaced

Subcellular locationi

GO - Cellular componenti

  1. endoplasmic reticulum Source: UniProtKB-KW
  2. membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane, Microsome

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi436 – 4361C → S: Conversion into an NADPH oxidase with negligible monooxygenase activity. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 491491Cytochrome P450 2B4PRO_0000051681Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei128 – 1281Phosphoserine; by PKABy similarity

Keywords - PTMi

Phosphoprotein

Expressioni

Inductioni

By phenobarbital.

Interactioni

Structurei

Secondary structure

1
491
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Turni38 – 403Combined sources
Helixi43 – 453Combined sources
Helixi51 – 6212Combined sources
Beta strandi64 – 707Combined sources
Beta strandi73 – 786Combined sources
Helixi81 – 888Combined sources
Turni89 – 957Combined sources
Helixi101 – 1044Combined sources
Helixi105 – 1084Combined sources
Helixi112 – 1154Combined sources
Helixi118 – 13417Combined sources
Turni135 – 1384Combined sources
Helixi139 – 15921Combined sources
Turni160 – 1623Combined sources
Beta strandi165 – 1673Combined sources
Helixi168 – 18417Combined sources
Helixi193 – 20513Combined sources
Turni206 – 2083Combined sources
Helixi213 – 2197Combined sources
Helixi221 – 2255Combined sources
Helixi231 – 25323Combined sources
Beta strandi258 – 2603Combined sources
Helixi264 – 27512Combined sources
Helixi282 – 31635Combined sources
Helixi318 – 33114Combined sources
Beta strandi334 – 3363Combined sources
Helixi340 – 3456Combined sources
Helixi347 – 36014Combined sources
Beta strandi362 – 3654Combined sources
Beta strandi375 – 3773Combined sources
Beta strandi380 – 3823Combined sources
Beta strandi387 – 3904Combined sources
Helixi392 – 3965Combined sources
Turni399 – 4013Combined sources
Beta strandi402 – 4043Combined sources
Helixi410 – 4134Combined sources
Beta strandi418 – 4203Combined sources
Helixi432 – 4343Combined sources
Helixi439 – 45618Combined sources
Beta strandi457 – 4604Combined sources
Helixi465 – 4673Combined sources
Beta strandi473 – 4808Combined sources
Beta strandi486 – 4905Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1PO5X-ray1.60A25-491[»]
1SUOX-ray1.90A25-491[»]
2BDMX-ray2.30A25-491[»]
2Q6NX-ray3.20A/B/C/D/E/F/G25-491[»]
3G5NX-ray2.50A/B/C/D25-491[»]
3G93X-ray3.20A/B/C/D25-491[»]
3KW4X-ray2.67A25-491[»]
3ME6X-ray3.10A/B/C/D25-491[»]
3MVRX-ray1.76A/B25-491[»]
3R1AX-ray3.50A/B/C/D/E/F/G/H25-491[»]
3R1BX-ray3.00A/B/C/D25-491[»]
3TK3X-ray2.80A/B/C/D25-491[»]
3TMZX-ray2.25A25-491[»]
3UASX-ray2.94A25-491[»]
4H1NX-ray2.99A25-491[»]
4JLTX-ray2.14A25-491[»]
4MGJX-ray2.41A30-491[»]
ProteinModelPortaliP00178.
SMRiP00178. Positions 28-491.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP00178.

Family & Domainsi

Sequence similaritiesi

Belongs to the cytochrome P450 family.Curated

Phylogenomic databases

eggNOGiCOG2124.
HOGENOMiHOG000036992.
HOVERGENiHBG015789.
InParanoidiP00178.

Family and domain databases

Gene3Di1.10.630.10. 1 hit.
InterProiIPR001128. Cyt_P450.
IPR017972. Cyt_P450_CS.
IPR002401. Cyt_P450_E_grp-I.
IPR008068. Cyt_P450_E_grp-I_CYP2B-like.
[Graphical view]
PfamiPF00067. p450. 1 hit.
[Graphical view]
PRINTSiPR00463. EP450I.
PR01685. EP450ICYP2B.
PR00385. P450.
SUPFAMiSSF48264. SSF48264. 1 hit.
PROSITEiPS00086. CYTOCHROME_P450. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P00178-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MEFSLLLLLA FLAGLLLLLF RGHPKAHGRL PPGPSPLPVL GNLLQMDRKG
60 70 80 90 100
LLRSFLRLRE KYGDVFTVYL GSRPVVVLCG TDAIREALVD QAEAFSGRGK
110 120 130 140 150
IAVVDPIFQG YGVIFANGER WRALRRFSLA TMRDFGMGKR SVEERIQEEA
160 170 180 190 200
RCLVEELRKS KGALLDNTLL FHSITSNIIC SIVFGKRFDY KDPVFLRLLD
210 220 230 240 250
LFFQSFSLIS SFSSQVFELF PGFLKHFPGT HRQIYRNLQE INTFIGQSVE
260 270 280 290 300
KHRATLDPSN PRDFIDVYLL RMEKDKSDPS SEFHHQNLIL TVLSLFFAGT
310 320 330 340 350
ETTSTTLRYG FLLMLKYPHV TERVQKEIEQ VIGSHRPPAL DDRAKMPYTD
360 370 380 390 400
AVIHEIQRLG DLIPFGVPHT VTKDTQFRGY VIPKNTEVFP VLSSALHDPR
410 420 430 440 450
YFETPNTFNP GHFLDANGAL KRNEGFMPFS LGKRICLGEG IARTELFLFF
460 470 480 490
TTILQNFSIA SPVPPEDIDL TPRESGVGNV PPSYQIRFLA R
Length:491
Mass (Da):55,713
Last modified:July 21, 1986 - v1
Checksum:i0DB943C6CDCF690B
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti91 – 911Q → E AA sequence (PubMed:6833251)Curated
Sequence conflicti95 – 962FS → SF AA sequence (PubMed:6833251)Curated
Sequence conflicti99 – 1002Missing AA sequence (PubMed:6833251)Curated
Sequence conflicti135 – 1362FG → GY AA sequence (PubMed:6833251)Curated
Sequence conflicti193 – 1931P → K AA sequence (PubMed:6833251)Curated
Sequence conflicti221 – 2211P → S AA sequence (PubMed:6833251)Curated
Sequence conflicti303 – 3031T → A AA sequence (PubMed:6833251)Curated
Sequence conflicti461 – 4655SPVPP → GNLSL AA sequence (PubMed:6833251)Curated

Polymorphismi

Types B0 and B1 are probably allelic variants.

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti39 – 391V → I in B1.
Natural varianti174 – 1741I → V in B1.
Natural varianti290 – 2901L → I in B1.
Natural varianti314 – 3141M → L in B1.
Natural varianti420 – 4201L → M in B1.

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M20856 mRNA. Translation: AAA65840.1.
M20857 mRNA. Translation: AAA31224.1.
PIRiA00179. O4RBPC.
S31277.
RefSeqiNP_001164602.1. NM_001171131.1.
UniGeneiOcu.1858.
Ocu.7479.

Genome annotation databases

GeneIDi100328948.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M20856 mRNA. Translation: AAA65840.1 .
M20857 mRNA. Translation: AAA31224.1 .
PIRi A00179. O4RBPC.
S31277.
RefSeqi NP_001164602.1. NM_001171131.1.
UniGenei Ocu.1858.
Ocu.7479.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1PO5 X-ray 1.60 A 25-491 [» ]
1SUO X-ray 1.90 A 25-491 [» ]
2BDM X-ray 2.30 A 25-491 [» ]
2Q6N X-ray 3.20 A/B/C/D/E/F/G 25-491 [» ]
3G5N X-ray 2.50 A/B/C/D 25-491 [» ]
3G93 X-ray 3.20 A/B/C/D 25-491 [» ]
3KW4 X-ray 2.67 A 25-491 [» ]
3ME6 X-ray 3.10 A/B/C/D 25-491 [» ]
3MVR X-ray 1.76 A/B 25-491 [» ]
3R1A X-ray 3.50 A/B/C/D/E/F/G/H 25-491 [» ]
3R1B X-ray 3.00 A/B/C/D 25-491 [» ]
3TK3 X-ray 2.80 A/B/C/D 25-491 [» ]
3TMZ X-ray 2.25 A 25-491 [» ]
3UAS X-ray 2.94 A 25-491 [» ]
4H1N X-ray 2.99 A 25-491 [» ]
4JLT X-ray 2.14 A 25-491 [» ]
4MGJ X-ray 2.41 A 30-491 [» ]
ProteinModelPortali P00178.
SMRi P00178. Positions 28-491.
ModBasei Search...
MobiDBi Search...

Chemistry

BindingDBi P00178.
ChEMBLi CHEMBL1743542.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 100328948.

Phylogenomic databases

eggNOGi COG2124.
HOGENOMi HOG000036992.
HOVERGENi HBG015789.
InParanoidi P00178.

Miscellaneous databases

EvolutionaryTracei P00178.

Family and domain databases

Gene3Di 1.10.630.10. 1 hit.
InterProi IPR001128. Cyt_P450.
IPR017972. Cyt_P450_CS.
IPR002401. Cyt_P450_E_grp-I.
IPR008068. Cyt_P450_E_grp-I_CYP2B-like.
[Graphical view ]
Pfami PF00067. p450. 1 hit.
[Graphical view ]
PRINTSi PR00463. EP450I.
PR01685. EP450ICYP2B.
PR00385. P450.
SUPFAMi SSF48264. SSF48264. 1 hit.
PROSITEi PS00086. CYTOCHROME_P450. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Primary structures of multiple forms of cytochrome P-450 isozyme 2 derived from rabbit pulmonary and hepatic cDNAs."
    Gasser R., Negishi M., Philpot R.M.
    Mol. Pharmacol. 33:22-30(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "Complete amino acid sequence and predicted membrane topology of phenobarbital-induced cytochrome P-450 (isozyme 2) from rabbit liver microsomes."
    Tarr G.E., Black S.D., Fujita V.S., Coon M.J.
    Proc. Natl. Acad. Sci. U.S.A. 80:6552-6556(1983) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE.
  3. "The complete amino acid sequence of rabbit phenobarbital-induced liver microsomal cytochrome P-450."
    Heinemann F.S., Ozols J.
    J. Biol. Chem. 258:4195-4201(1983) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE.
  4. "Cytochrome P-450 isozymes 2 and 5 in rabbit lung and liver. Comparisons of structure and inducibility."
    Parandoosh Z., Fujita V.S., Coon M.J., Philpot R.M.
    Drug Metab. Dispos. 15:59-67(1987) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 1-24.
  5. "Microheterogeneity in the major phenobarbital-inducible forms of rabbit liver microsomal cytochrome P-450 as revealed by nucleotide sequencing of cloned cDNAs."
    Komori M., Imai Y., Tsunasawa S., Sato R.
    Biochemistry 27:73-80(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 301-491 (B1).
  6. "Replacement of active-site cysteine-436 by serine converts cytochrome P450 2B4 into an NADPH oxidase with negligible monooxygenase activity."
    Vatsis K.P., Peng H.-M., Coon M.J.
    J. Inorg. Biochem. 91:542-553(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: MUTAGENESIS OF CYS-436.
  7. Cited for: X-RAY CRYSTALLOGRAPHY (1.6 ANGSTROMS).

Entry informationi

Entry nameiCP2B4_RABIT
AccessioniPrimary (citable) accession number: P00178
Secondary accession number(s): P00177
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: July 21, 1986
Last modified: November 26, 2014
This is version 130 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3