Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

P00178

- CP2B4_RABIT

UniProt

P00178 - CP2B4_RABIT

Protein

Cytochrome P450 2B4

Gene

CYP2B4

Organism
Oryctolagus cuniculus (Rabbit)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 128 (01 Oct 2014)
      Sequence version 1 (21 Jul 1986)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Cytochromes P450 are a group of heme-thiolate monooxygenases. In liver microsomes, this enzyme is involved in an NADPH-dependent electron transport pathway. It oxidizes a variety of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics. In the epoxidation of arachidonic acid it has a unique preference for the 5,6-olefin.

    Catalytic activityi

    RH + reduced flavoprotein + O2 = ROH + oxidized flavoprotein + H2O.

    Cofactori

    Heme group.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi436 – 4361Iron (heme axial ligand)

    GO - Molecular functioni

    1. aromatase activity Source: UniProtKB-EC
    2. heme binding Source: InterPro
    3. iron ion binding Source: InterPro

    Keywords - Molecular functioni

    Monooxygenase, Oxidoreductase

    Keywords - Ligandi

    Heme, Iron, Metal-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Cytochrome P450 2B4 (EC:1.14.14.1)
    Alternative name(s):
    CYPIIB4
    Cytochrome P450 isozyme 2
    Short name:
    Cytochrome P450 LM2
    Cytochrome P450 type B0
    Cytochrome P450 type B1
    Gene namesi
    Name:CYP2B4
    OrganismiOryctolagus cuniculus (Rabbit)
    Taxonomic identifieri9986 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresLagomorphaLeporidaeOryctolagus
    ProteomesiUP000001811: Unplaced

    Subcellular locationi

    GO - Cellular componenti

    1. endoplasmic reticulum membrane Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Endoplasmic reticulum, Membrane, Microsome

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi436 – 4361C → S: Conversion into an NADPH oxidase with negligible monooxygenase activity. 1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 491491Cytochrome P450 2B4PRO_0000051681Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei128 – 1281Phosphoserine; by PKABy similarity

    Keywords - PTMi

    Phosphoprotein

    Expressioni

    Inductioni

    By phenobarbital.

    Structurei

    Secondary structure

    1
    491
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Turni38 – 403
    Helixi43 – 453
    Helixi51 – 6212
    Beta strandi64 – 707
    Beta strandi73 – 786
    Helixi81 – 888
    Turni89 – 957
    Helixi101 – 1044
    Helixi105 – 1084
    Helixi112 – 1154
    Helixi118 – 13417
    Turni135 – 1384
    Helixi139 – 15921
    Turni160 – 1623
    Beta strandi165 – 1673
    Helixi168 – 18417
    Helixi193 – 20513
    Turni206 – 2083
    Helixi213 – 2197
    Helixi221 – 2255
    Helixi231 – 25323
    Beta strandi258 – 2603
    Helixi264 – 27512
    Helixi282 – 31635
    Helixi318 – 33114
    Beta strandi334 – 3363
    Helixi340 – 3456
    Helixi347 – 36014
    Beta strandi362 – 3654
    Beta strandi375 – 3773
    Beta strandi380 – 3823
    Beta strandi387 – 3904
    Helixi392 – 3965
    Turni399 – 4013
    Beta strandi402 – 4043
    Helixi410 – 4134
    Beta strandi418 – 4203
    Helixi432 – 4343
    Helixi439 – 45618
    Beta strandi457 – 4604
    Helixi465 – 4673
    Beta strandi473 – 4808
    Beta strandi486 – 4905

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1PO5X-ray1.60A25-491[»]
    1SUOX-ray1.90A25-491[»]
    2BDMX-ray2.30A25-491[»]
    2Q6NX-ray3.20A/B/C/D/E/F/G25-491[»]
    3G5NX-ray2.50A/B/C/D25-491[»]
    3G93X-ray3.20A/B/C/D25-491[»]
    3KW4X-ray2.67A25-491[»]
    3ME6X-ray3.10A/B/C/D25-491[»]
    3MVRX-ray1.76A/B25-491[»]
    3R1AX-ray3.50A/B/C/D/E/F/G/H25-491[»]
    3R1BX-ray3.00A/B/C/D25-491[»]
    3TK3X-ray2.80A/B/C/D25-491[»]
    3TMZX-ray2.25A25-491[»]
    3UASX-ray2.94A25-491[»]
    4H1NX-ray2.99A25-491[»]
    4JLTX-ray2.14A25-491[»]
    ProteinModelPortaliP00178.
    SMRiP00178. Positions 28-491.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP00178.

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the cytochrome P450 family.Curated

    Phylogenomic databases

    eggNOGiCOG2124.
    HOGENOMiHOG000036992.
    HOVERGENiHBG015789.

    Family and domain databases

    Gene3Di1.10.630.10. 1 hit.
    InterProiIPR001128. Cyt_P450.
    IPR017972. Cyt_P450_CS.
    IPR002401. Cyt_P450_E_grp-I.
    IPR008068. Cyt_P450_E_grp-I_CYP2B-like.
    [Graphical view]
    PfamiPF00067. p450. 1 hit.
    [Graphical view]
    PRINTSiPR00463. EP450I.
    PR01685. EP450ICYP2B.
    PR00385. P450.
    SUPFAMiSSF48264. SSF48264. 1 hit.
    PROSITEiPS00086. CYTOCHROME_P450. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P00178-1 [UniParc]FASTAAdd to Basket

    « Hide

    MEFSLLLLLA FLAGLLLLLF RGHPKAHGRL PPGPSPLPVL GNLLQMDRKG    50
    LLRSFLRLRE KYGDVFTVYL GSRPVVVLCG TDAIREALVD QAEAFSGRGK 100
    IAVVDPIFQG YGVIFANGER WRALRRFSLA TMRDFGMGKR SVEERIQEEA 150
    RCLVEELRKS KGALLDNTLL FHSITSNIIC SIVFGKRFDY KDPVFLRLLD 200
    LFFQSFSLIS SFSSQVFELF PGFLKHFPGT HRQIYRNLQE INTFIGQSVE 250
    KHRATLDPSN PRDFIDVYLL RMEKDKSDPS SEFHHQNLIL TVLSLFFAGT 300
    ETTSTTLRYG FLLMLKYPHV TERVQKEIEQ VIGSHRPPAL DDRAKMPYTD 350
    AVIHEIQRLG DLIPFGVPHT VTKDTQFRGY VIPKNTEVFP VLSSALHDPR 400
    YFETPNTFNP GHFLDANGAL KRNEGFMPFS LGKRICLGEG IARTELFLFF 450
    TTILQNFSIA SPVPPEDIDL TPRESGVGNV PPSYQIRFLA R 491
    Length:491
    Mass (Da):55,713
    Last modified:July 21, 1986 - v1
    Checksum:i0DB943C6CDCF690B
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti91 – 911Q → E AA sequence (PubMed:6833251)Curated
    Sequence conflicti95 – 962FS → SF AA sequence (PubMed:6833251)Curated
    Sequence conflicti99 – 1002Missing AA sequence (PubMed:6833251)Curated
    Sequence conflicti135 – 1362FG → GY AA sequence (PubMed:6833251)Curated
    Sequence conflicti193 – 1931P → K AA sequence (PubMed:6833251)Curated
    Sequence conflicti221 – 2211P → S AA sequence (PubMed:6833251)Curated
    Sequence conflicti303 – 3031T → A AA sequence (PubMed:6833251)Curated
    Sequence conflicti461 – 4655SPVPP → GNLSL AA sequence (PubMed:6833251)Curated

    Polymorphismi

    Types B0 and B1 are probably allelic variants.

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti39 – 391V → I in B1.
    Natural varianti174 – 1741I → V in B1.
    Natural varianti290 – 2901L → I in B1.
    Natural varianti314 – 3141M → L in B1.
    Natural varianti420 – 4201L → M in B1.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M20856 mRNA. Translation: AAA65840.1.
    M20857 mRNA. Translation: AAA31224.1.
    PIRiA00179. O4RBPC.
    S31277.
    RefSeqiNP_001164602.1. NM_001171131.1.
    UniGeneiOcu.1858.
    Ocu.7479.

    Genome annotation databases

    GeneIDi100328948.

    Keywords - Coding sequence diversityi

    Polymorphism

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M20856 mRNA. Translation: AAA65840.1 .
    M20857 mRNA. Translation: AAA31224.1 .
    PIRi A00179. O4RBPC.
    S31277.
    RefSeqi NP_001164602.1. NM_001171131.1.
    UniGenei Ocu.1858.
    Ocu.7479.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1PO5 X-ray 1.60 A 25-491 [» ]
    1SUO X-ray 1.90 A 25-491 [» ]
    2BDM X-ray 2.30 A 25-491 [» ]
    2Q6N X-ray 3.20 A/B/C/D/E/F/G 25-491 [» ]
    3G5N X-ray 2.50 A/B/C/D 25-491 [» ]
    3G93 X-ray 3.20 A/B/C/D 25-491 [» ]
    3KW4 X-ray 2.67 A 25-491 [» ]
    3ME6 X-ray 3.10 A/B/C/D 25-491 [» ]
    3MVR X-ray 1.76 A/B 25-491 [» ]
    3R1A X-ray 3.50 A/B/C/D/E/F/G/H 25-491 [» ]
    3R1B X-ray 3.00 A/B/C/D 25-491 [» ]
    3TK3 X-ray 2.80 A/B/C/D 25-491 [» ]
    3TMZ X-ray 2.25 A 25-491 [» ]
    3UAS X-ray 2.94 A 25-491 [» ]
    4H1N X-ray 2.99 A 25-491 [» ]
    4JLT X-ray 2.14 A 25-491 [» ]
    ProteinModelPortali P00178.
    SMRi P00178. Positions 28-491.
    ModBasei Search...
    MobiDBi Search...

    Chemistry

    ChEMBLi CHEMBL1743542.
    DrugBanki DB04794. Bifonazole.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 100328948.

    Phylogenomic databases

    eggNOGi COG2124.
    HOGENOMi HOG000036992.
    HOVERGENi HBG015789.

    Miscellaneous databases

    EvolutionaryTracei P00178.

    Family and domain databases

    Gene3Di 1.10.630.10. 1 hit.
    InterProi IPR001128. Cyt_P450.
    IPR017972. Cyt_P450_CS.
    IPR002401. Cyt_P450_E_grp-I.
    IPR008068. Cyt_P450_E_grp-I_CYP2B-like.
    [Graphical view ]
    Pfami PF00067. p450. 1 hit.
    [Graphical view ]
    PRINTSi PR00463. EP450I.
    PR01685. EP450ICYP2B.
    PR00385. P450.
    SUPFAMi SSF48264. SSF48264. 1 hit.
    PROSITEi PS00086. CYTOCHROME_P450. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Primary structures of multiple forms of cytochrome P-450 isozyme 2 derived from rabbit pulmonary and hepatic cDNAs."
      Gasser R., Negishi M., Philpot R.M.
      Mol. Pharmacol. 33:22-30(1988) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    2. "Complete amino acid sequence and predicted membrane topology of phenobarbital-induced cytochrome P-450 (isozyme 2) from rabbit liver microsomes."
      Tarr G.E., Black S.D., Fujita V.S., Coon M.J.
      Proc. Natl. Acad. Sci. U.S.A. 80:6552-6556(1983) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE.
    3. "The complete amino acid sequence of rabbit phenobarbital-induced liver microsomal cytochrome P-450."
      Heinemann F.S., Ozols J.
      J. Biol. Chem. 258:4195-4201(1983) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE.
    4. "Cytochrome P-450 isozymes 2 and 5 in rabbit lung and liver. Comparisons of structure and inducibility."
      Parandoosh Z., Fujita V.S., Coon M.J., Philpot R.M.
      Drug Metab. Dispos. 15:59-67(1987) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 1-24.
    5. "Microheterogeneity in the major phenobarbital-inducible forms of rabbit liver microsomal cytochrome P-450 as revealed by nucleotide sequencing of cloned cDNAs."
      Komori M., Imai Y., Tsunasawa S., Sato R.
      Biochemistry 27:73-80(1988) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 301-491 (B1).
    6. "Replacement of active-site cysteine-436 by serine converts cytochrome P450 2B4 into an NADPH oxidase with negligible monooxygenase activity."
      Vatsis K.P., Peng H.-M., Coon M.J.
      J. Inorg. Biochem. 91:542-553(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: MUTAGENESIS OF CYS-436.
    7. Cited for: X-RAY CRYSTALLOGRAPHY (1.6 ANGSTROMS).

    Entry informationi

    Entry nameiCP2B4_RABIT
    AccessioniPrimary (citable) accession number: P00178
    Secondary accession number(s): P00177
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 21, 1986
    Last sequence update: July 21, 1986
    Last modified: October 1, 2014
    This is version 128 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3