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Protein

Cytochrome P450 2B4

Gene

CYP2B4

Organism
Oryctolagus cuniculus (Rabbit)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Cytochromes P450 are a group of heme-thiolate monooxygenases. In liver microsomes, this enzyme is involved in an NADPH-dependent electron transport pathway. It oxidizes a variety of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics. In the epoxidation of arachidonic acid it has a unique preference for the 5,6-olefin.

Catalytic activityi

RH + [reduced NADPH--hemoprotein reductase] + O2 = ROH + [oxidized NADPH--hemoprotein reductase] + H2O.

Cofactori

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi436Iron (heme axial ligand)1

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Monooxygenase, Oxidoreductase

Keywords - Ligandi

Heme, Iron, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Cytochrome P450 2B4 (EC:1.14.14.1)
Alternative name(s):
CYPIIB4
Cytochrome P450 isozyme 2
Short name:
Cytochrome P450 LM2
Cytochrome P450 type B0
Cytochrome P450 type B1
Gene namesi
Name:CYP2B4
OrganismiOryctolagus cuniculus (Rabbit)
Taxonomic identifieri9986 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresLagomorphaLeporidaeOryctolagus
Proteomesi
  • UP000001811 Componenti: Unplaced

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane, Microsome

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi436C → S: Conversion into an NADPH oxidase with negligible monooxygenase activity. 1 Publication1

Chemistry databases

ChEMBLiCHEMBL1743542.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000516811 – 491Cytochrome P450 2B4Add BLAST491

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei128Phosphoserine; by PKABy similarity1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PRIDEiP00178.

Expressioni

Inductioni

By phenobarbital.

Interactioni

Protein-protein interaction databases

STRINGi9986.ENSOCUP00000022188.

Chemistry databases

BindingDBiP00178.

Structurei

Secondary structure

1491
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Turni38 – 40Combined sources3
Helixi43 – 45Combined sources3
Helixi51 – 62Combined sources12
Beta strandi64 – 70Combined sources7
Beta strandi73 – 78Combined sources6
Helixi81 – 88Combined sources8
Turni89 – 95Combined sources7
Helixi101 – 104Combined sources4
Helixi105 – 108Combined sources4
Helixi112 – 115Combined sources4
Helixi118 – 134Combined sources17
Turni135 – 138Combined sources4
Helixi139 – 159Combined sources21
Turni160 – 162Combined sources3
Beta strandi165 – 167Combined sources3
Helixi168 – 184Combined sources17
Helixi193 – 205Combined sources13
Turni206 – 208Combined sources3
Helixi213 – 219Combined sources7
Helixi221 – 225Combined sources5
Helixi231 – 253Combined sources23
Beta strandi258 – 260Combined sources3
Helixi264 – 275Combined sources12
Helixi282 – 316Combined sources35
Helixi318 – 331Combined sources14
Beta strandi334 – 336Combined sources3
Helixi340 – 345Combined sources6
Helixi347 – 360Combined sources14
Beta strandi362 – 365Combined sources4
Beta strandi375 – 377Combined sources3
Beta strandi380 – 382Combined sources3
Beta strandi387 – 390Combined sources4
Helixi392 – 396Combined sources5
Turni399 – 401Combined sources3
Beta strandi402 – 404Combined sources3
Helixi410 – 413Combined sources4
Beta strandi418 – 420Combined sources3
Helixi432 – 434Combined sources3
Helixi439 – 456Combined sources18
Beta strandi457 – 460Combined sources4
Helixi465 – 467Combined sources3
Beta strandi473 – 480Combined sources8
Beta strandi486 – 490Combined sources5

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1PO5X-ray1.60A25-491[»]
1SUOX-ray1.90A25-491[»]
2BDMX-ray2.30A25-491[»]
2Q6NX-ray3.20A/B/C/D/E/F/G25-491[»]
3G5NX-ray2.50A/B/C/D25-491[»]
3G93X-ray3.20A/B/C/D25-491[»]
3KW4X-ray2.67A25-491[»]
3ME6X-ray3.10A/B/C/D25-491[»]
3MVRX-ray1.76A/B25-491[»]
3R1AX-ray3.50A/B/C/D/E/F/G/H25-491[»]
3R1BX-ray3.00A/B/C/D25-491[»]
3TK3X-ray2.80A/B/C/D25-491[»]
3TMZX-ray2.25A25-491[»]
3UASX-ray2.94A25-491[»]
4H1NX-ray2.99A25-491[»]
4JLTX-ray2.14A25-491[»]
4MGJX-ray2.41A30-491[»]
5EM4X-ray3.02A/B1-491[»]
5IUTX-ray2.34A25-491[»]
5IUZX-ray2.73A/B25-491[»]
ProteinModelPortaliP00178.
SMRiP00178.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP00178.

Family & Domainsi

Sequence similaritiesi

Belongs to the cytochrome P450 family.Curated

Phylogenomic databases

eggNOGiKOG0156. Eukaryota.
COG2124. LUCA.
HOGENOMiHOG000036992.
HOVERGENiHBG015789.
InParanoidiP00178.

Family and domain databases

Gene3Di1.10.630.10. 1 hit.
InterProiIPR001128. Cyt_P450.
IPR017972. Cyt_P450_CS.
IPR002401. Cyt_P450_E_grp-I.
IPR008068. Cyt_P450_E_grp-I_CYP2B-like.
[Graphical view]
PfamiPF00067. p450. 1 hit.
[Graphical view]
PRINTSiPR00463. EP450I.
PR01685. EP450ICYP2B.
PR00385. P450.
SUPFAMiSSF48264. SSF48264. 1 hit.
PROSITEiPS00086. CYTOCHROME_P450. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P00178-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEFSLLLLLA FLAGLLLLLF RGHPKAHGRL PPGPSPLPVL GNLLQMDRKG
60 70 80 90 100
LLRSFLRLRE KYGDVFTVYL GSRPVVVLCG TDAIREALVD QAEAFSGRGK
110 120 130 140 150
IAVVDPIFQG YGVIFANGER WRALRRFSLA TMRDFGMGKR SVEERIQEEA
160 170 180 190 200
RCLVEELRKS KGALLDNTLL FHSITSNIIC SIVFGKRFDY KDPVFLRLLD
210 220 230 240 250
LFFQSFSLIS SFSSQVFELF PGFLKHFPGT HRQIYRNLQE INTFIGQSVE
260 270 280 290 300
KHRATLDPSN PRDFIDVYLL RMEKDKSDPS SEFHHQNLIL TVLSLFFAGT
310 320 330 340 350
ETTSTTLRYG FLLMLKYPHV TERVQKEIEQ VIGSHRPPAL DDRAKMPYTD
360 370 380 390 400
AVIHEIQRLG DLIPFGVPHT VTKDTQFRGY VIPKNTEVFP VLSSALHDPR
410 420 430 440 450
YFETPNTFNP GHFLDANGAL KRNEGFMPFS LGKRICLGEG IARTELFLFF
460 470 480 490
TTILQNFSIA SPVPPEDIDL TPRESGVGNV PPSYQIRFLA R
Length:491
Mass (Da):55,713
Last modified:July 21, 1986 - v1
Checksum:i0DB943C6CDCF690B
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti91Q → E AA sequence (PubMed:6833251).Curated1
Sequence conflicti95 – 96FS → SF AA sequence (PubMed:6833251).Curated2
Sequence conflicti99 – 100Missing AA sequence (PubMed:6833251).Curated2
Sequence conflicti135 – 136FG → GY AA sequence (PubMed:6833251).Curated2
Sequence conflicti193P → K AA sequence (PubMed:6833251).Curated1
Sequence conflicti221P → S AA sequence (PubMed:6833251).Curated1
Sequence conflicti303T → A AA sequence (PubMed:6833251).Curated1
Sequence conflicti461 – 465SPVPP → GNLSL AA sequence (PubMed:6833251).Curated5

Polymorphismi

Types B0 and B1 are probably allelic variants.

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural varianti39V → I in B1. 1
Natural varianti174I → V in B1. 1
Natural varianti290L → I in B1. 1
Natural varianti314M → L in B1. 1
Natural varianti420L → M in B1. 1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M20856 mRNA. Translation: AAA65840.1.
M20857 mRNA. Translation: AAA31224.1.
PIRiA00179. O4RBPC.
S31277.
RefSeqiNP_001164602.1. NM_001171131.1.
UniGeneiOcu.1858.
Ocu.7479.

Genome annotation databases

GeneIDi100328948.
KEGGiocu:100328948.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M20856 mRNA. Translation: AAA65840.1.
M20857 mRNA. Translation: AAA31224.1.
PIRiA00179. O4RBPC.
S31277.
RefSeqiNP_001164602.1. NM_001171131.1.
UniGeneiOcu.1858.
Ocu.7479.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1PO5X-ray1.60A25-491[»]
1SUOX-ray1.90A25-491[»]
2BDMX-ray2.30A25-491[»]
2Q6NX-ray3.20A/B/C/D/E/F/G25-491[»]
3G5NX-ray2.50A/B/C/D25-491[»]
3G93X-ray3.20A/B/C/D25-491[»]
3KW4X-ray2.67A25-491[»]
3ME6X-ray3.10A/B/C/D25-491[»]
3MVRX-ray1.76A/B25-491[»]
3R1AX-ray3.50A/B/C/D/E/F/G/H25-491[»]
3R1BX-ray3.00A/B/C/D25-491[»]
3TK3X-ray2.80A/B/C/D25-491[»]
3TMZX-ray2.25A25-491[»]
3UASX-ray2.94A25-491[»]
4H1NX-ray2.99A25-491[»]
4JLTX-ray2.14A25-491[»]
4MGJX-ray2.41A30-491[»]
5EM4X-ray3.02A/B1-491[»]
5IUTX-ray2.34A25-491[»]
5IUZX-ray2.73A/B25-491[»]
ProteinModelPortaliP00178.
SMRiP00178.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9986.ENSOCUP00000022188.

Chemistry databases

BindingDBiP00178.
ChEMBLiCHEMBL1743542.

Proteomic databases

PRIDEiP00178.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi100328948.
KEGGiocu:100328948.

Phylogenomic databases

eggNOGiKOG0156. Eukaryota.
COG2124. LUCA.
HOGENOMiHOG000036992.
HOVERGENiHBG015789.
InParanoidiP00178.

Miscellaneous databases

EvolutionaryTraceiP00178.
PROiP00178.

Family and domain databases

Gene3Di1.10.630.10. 1 hit.
InterProiIPR001128. Cyt_P450.
IPR017972. Cyt_P450_CS.
IPR002401. Cyt_P450_E_grp-I.
IPR008068. Cyt_P450_E_grp-I_CYP2B-like.
[Graphical view]
PfamiPF00067. p450. 1 hit.
[Graphical view]
PRINTSiPR00463. EP450I.
PR01685. EP450ICYP2B.
PR00385. P450.
SUPFAMiSSF48264. SSF48264. 1 hit.
PROSITEiPS00086. CYTOCHROME_P450. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCP2B4_RABIT
AccessioniPrimary (citable) accession number: P00178
Secondary accession number(s): P00177
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: July 21, 1986
Last modified: November 2, 2016
This is version 144 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.