P00174 (CYB5_CHICK) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Cytochrome b5

Gene names

Name:	CYB5A
Synonyms:	CYB5

Organism

Gallus gallus (Chicken)

Taxonomic identifier

9031 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Archosauria › Dinosauria › Saurischia › Theropoda › Coelurosauria › Aves › Neognathae › Galliformes › Phasianidae › Phasianinae › Gallus

Protein attributes

Sequence length	138 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Cytochrome b5 is a membrane bound hemoprotein which function as an electron carrier for several membrane bound oxygenases.
Subcellular location	Endoplasmic reticulum membrane; Single-pass membrane protein; Cytoplasmic side. Microsome membrane; Single-pass membrane protein; Cytoplasmic side.
Sequence similarities	Belongs to the cytochrome b5 family. Contains 1 cytochrome b5 heme-binding domain.

Ontologies

Keywords
Biological process	Electron transport Transport
Cellular component	Endoplasmic reticulum Membrane Microsome
Domain	Transmembrane Transmembrane helix
Ligand	Heme Iron Metal-binding
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological process	electron transport chain Inferred from electronic annotation. Source: UniProtKB-KW transport Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	endoplasmic reticulum membrane Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW microsome Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	heme binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 138	138	Cytochrome b5		PRO_0000166015
Regions
	Transmembrane	114 – 136	23	Helical; Potential
	Domain	14 – 90	77	Cytochrome b5 heme-binding
Sites
	Metal binding	49	1	Iron (heme axial ligand)
	Metal binding	73	1	Iron (heme axial ligand)
Experimental info
	Sequence conflict	12	1	W → E AA sequence Ref.4
	Sequence conflict	27 – 29	3	NSQ → ZSB AA sequence Ref.3
	Sequence conflict	67	1	N → D AA sequence Ref.3
	Sequence conflict	124	1	A → T AA sequence Ref.4
	Sequence conflict	138	1	E → EE AA sequence Ref.4

Sequences

	Sequence		Length	Mass (Da)
	P00174 [UniParc]. Last modified August 1, 1990. Version 4. Checksum: 168F0B87251557A8	FASTA	138	15,545
	10 20 30 40 50 60 MVGSSEAGGE AWRGRYYRLE EVQKHNNSQS TWIIVHHRIY DITKFLDEHP GGEEVLREQA 70 80 90 100 110 120 GGDATENFED VGHSTDARAL SETFIIGELH PDDRPKLQKP AETLITTVQS NSSSWSNWVI 130 PAIAAIIVAL MYRSYMSE

References

[1]	"Soluble and membrane-bound forms of cytochrome b5 are the products of a single gene in chicken." Zhang H., Somerville C. Arch. Biochem. Biophys. 280:412-415(1990) [PubMed: 2369133] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"The primary structure of chicken liver cytochrome b5 deduced from the DNA sequence of a cDNA clone." Zhang H., Somerville C. Arch. Biochem. Biophys. 264:343-347(1988) [PubMed: 3395128] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[3]	"Amino acid sequences of tryptic peptides of cytochromes b5 from microsomes of human, monkey, porcine, and chicken liver." Nobrega F.G., Ozols J. J. Biol. Chem. 246:1706-1717(1971) [PubMed: 4993957] [Abstract] Cited for: PROTEIN SEQUENCE OF 14-97.
[4]	"Structure of cytochrome b5 and its topology in the microsomal membrane." Ozols J. Biochim. Biophys. Acta 997:121-130(1989) [PubMed: 2752049] [Abstract] Cited for: PROTEIN SEQUENCE OF 4-16 AND 89-138.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M32293 mRNA. Translation: AAA48740.1. M18539 mRNA. Translation: AAA48733.1.
IPI	IPI00595035.
PIR	CBCH5. A28811.
RefSeq	NP_001001748.1. NM_001001748.1.
UniGene	Gga.4989.
3D structure databases
ProteinModelPortal	P00174.
SMR	P00174. Positions 15-113.
ModBase	Search...
Proteomic databases
PRIDE	P00174.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSGALT00000022289; ENSGALP00000022250; ENSGALG00000013708.
GeneID	414798.
KEGG	gga:414798.
Organism-specific databases
CTD	1528.
Phylogenomic databases
eggNOG	veNOG19867.
GeneTree	ENSGT00510000046507.
HOGENOM	HBG629516.
HOVERGEN	HBG002653.
InParanoid	P00174.
OMA	AVALMYR.
OrthoDB	EOG4S7JRC.
PhylomeDB	P00174.
Family and domain databases
InterPro	IPR001199. Cyt_B5. IPR018506. Cyt_B5_heme-BS. [Graphical view]
Gene3D	G3DSA:3.10.120.10. Cyt_B5. 1 hit.
Pfam	PF00173. Cyt-b5. 1 hit. [Graphical view]
PRINTS	PR00363. CYTOCHROMEB5.
SUPFAM	SSF55856. Cyt_B5. 1 hit.
PROSITE	PS00191. CYTOCHROME_B5_1. 1 hit. PS50255. CYTOCHROME_B5_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CYB5_CHICK

Accession

Primary (citable) accession number: P00174

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 21, 1986
Last sequence update:	August 1, 1990
Last modified:	September 21, 2011
This is version 105 of the entry and version 4 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program