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Protein

Cytochrome b5

Gene

CYB5A

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Cytochrome b5 is a membrane bound hemoprotein which function as an electron carrier for several membrane bound oxygenases.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi44Iron (heme axial ligand)PROSITE-ProRule annotation1 Publication1
Metal bindingi68Iron (heme axial ligand)PROSITE-ProRule annotation1 Publication1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Biological processElectron transport, Transport
LigandHeme, Iron, Metal-binding

Enzyme and pathway databases

ReactomeiR-BTA-196836 Vitamin C (ascorbate) metabolism
SABIO-RKiP00171

Names & Taxonomyi

Protein namesi
Recommended name:
Cytochrome b5
Gene namesi
Name:CYB5A
Synonyms:CYB5
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
Proteomesi
  • UP000009136 Componenti: Chromosome 24

Organism-specific databases

VGNCiVGNC:50260 CYB5A

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei109 – 131HelicalSequence analysisAdd BLAST23

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane, Microsome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved3 Publications
ChainiPRO_00001660082 – 134Cytochrome b5Add BLAST133

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylalanine2 Publications1
Modified residuei7N6-acetyllysineBy similarity1
Modified residuei10N6-acetyllysineBy similarity1
Modified residuei19N6-acetyllysineBy similarity1

Keywords - PTMi

Acetylation

Proteomic databases

PaxDbiP00171
PeptideAtlasiP00171
PRIDEiP00171

PTM databases

iPTMnetiP00171

Expressioni

Gene expression databases

BgeeiENSBTAG00000012012

Interactioni

GO - Molecular functioni

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000015944

Structurei

Secondary structure

1134
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi9 – 12Combined sources4
Helixi14 – 17Combined sources4
Beta strandi21 – 24Combined sources4
Beta strandi25 – 30Combined sources6
Beta strandi33 – 36Combined sources4
Turni38 – 43Combined sources6
Helixi49 – 54Combined sources6
Beta strandi56 – 58Combined sources3
Helixi60 – 65Combined sources6
Helixi70 – 76Combined sources7
Turni77 – 79Combined sources3
Beta strandi80 – 84Combined sources5
Helixi86 – 91Combined sources6

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1CYOX-ray1.50A6-98[»]
1EHBX-ray1.90A8-89[»]
1ES1X-ray2.10A8-89[»]
1F03NMR-A8-89[»]
1F04NMR-A8-89[»]
1HKONMR-A2-105[»]
1I5UNMR-A8-89[»]
1J0QNMR-A8-89[»]
1LQXX-ray1.80A8-89[»]
1LR6X-ray1.90A8-89[»]
1M20X-ray1.80A8-89[»]
1M2IX-ray1.80A8-89[»]
1M2MX-ray1.80A8-89[»]
1M59X-ray1.90A8-89[»]
1NX7NMR-A8-89[»]
1SH4NMR-A8-89[»]
1U9MX-ray2.00A/B/C/D/E/F8-89[»]
1U9UX-ray1.86A8-89[»]
3OZZX-ray1.70B8-89[»]
4HINX-ray2.40A/B/C/D8-89[»]
ProteinModelPortaliP00171
SMRiP00171
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP00171

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini9 – 85Cytochrome b5 heme-bindingPROSITE-ProRule annotationAdd BLAST77

Sequence similaritiesi

Belongs to the cytochrome b5 family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG0537 Eukaryota
COG5274 LUCA
GeneTreeiENSGT00510000046507
HOGENOMiHOG000039853
HOVERGENiHBG002653
InParanoidiP00171
OMAiFKSTWII
OrthoDBiEOG091G17G8
TreeFamiTF314537

Family and domain databases

Gene3Di3.10.120.10, 1 hit
InterProiView protein in InterPro
IPR001199 Cyt_B5-like_heme/steroid-bd
IPR036400 Cyt_B5-like_heme/steroid_sf
IPR018506 Cyt_B5_heme-BS
PfamiView protein in Pfam
PF00173 Cyt-b5, 1 hit
PRINTSiPR00363 CYTOCHROMEB5
SMARTiView protein in SMART
SM01117 Cyt-b5, 1 hit
SUPFAMiSSF55856 SSF55856, 1 hit
PROSITEiView protein in PROSITE
PS00191 CYTOCHROME_B5_1, 1 hit
PS50255 CYTOCHROME_B5_2, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P00171-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAEESSKAVK YYTLEEIQKH NNSKSTWLIL HYKVYDLTKF LEEHPGGEEV
60 70 80 90 100
LREQAGGDAT ENFEDVGHST DARELSKTFI IGELHPDDRS KITKPSESII
110 120 130
TTIDSNPSWW TNWLIPAISA LFVALIYHLY TSEN
Length:134
Mass (Da):15,329
Last modified:January 23, 2007 - v3
Checksum:i7B7B605158D97525
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti2 – 5AEES → ZSZZBA AA sequence (PubMed:4810060).Curated4
Sequence conflicti16 – 18EIQ → QIE AA sequence (PubMed:5391285).Curated3
Sequence conflicti18Q → E AA sequence (PubMed:5272324).Curated1
Sequence conflicti62N → D AA sequence (PubMed:5391285).Curated1
Sequence conflicti62N → D AA sequence (PubMed:5272324).Curated1
Sequence conflicti134N → D AA sequence (PubMed:4810060).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X13617 mRNA Translation: CAA31949.1
M63328, M63326, M63327 Genomic DNA Translation: AAC14455.1 Sequence problems.
L22966 Genomic DNA No translation available.
BC108113 mRNA Translation: AAI08114.1
PIRiA47215 CBBO5
RefSeqiNP_776458.1, NM_174033.3
UniGeneiBt.65097

Genome annotation databases

EnsembliENSBTAT00000015944; ENSBTAP00000015944; ENSBTAG00000012012
GeneIDi281110
KEGGibta:281110

Similar proteinsi

Entry informationi

Entry nameiCYB5_BOVIN
AccessioniPrimary (citable) accession number: P00171
Secondary accession number(s): Q27947, Q28837, Q32PH5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: January 23, 2007
Last modified: May 23, 2018
This is version 166 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

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