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Protein

Cytochrome b5

Gene

CYB5A

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Cytochrome b5 is a membrane bound hemoprotein which function as an electron carrier for several membrane bound oxygenases.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi44Iron (heme axial ligand)PROSITE-ProRule annotation1 Publication1
Metal bindingi68Iron (heme axial ligand)PROSITE-ProRule annotation1 Publication1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Electron transport, Transport

Keywords - Ligandi

Heme, Iron, Metal-binding

Enzyme and pathway databases

ReactomeiR-BTA-196836. Vitamin C (ascorbate) metabolism.

Names & Taxonomyi

Protein namesi
Recommended name:
Cytochrome b5
Gene namesi
Name:CYB5A
Synonyms:CYB5
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
Proteomesi
  • UP000009136 Componenti: Chromosome 24

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei109 – 131HelicalSequence analysisAdd BLAST23

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane, Microsome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved3 Publications
ChainiPRO_00001660082 – 134Cytochrome b5Add BLAST133

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylalanine2 Publications1
Modified residuei7N6-acetyllysineBy similarity1
Modified residuei10N6-acetyllysineBy similarity1
Modified residuei19N6-acetyllysineBy similarity1

Keywords - PTMi

Acetylation

Proteomic databases

PaxDbiP00171.
PeptideAtlasiP00171.
PRIDEiP00171.

Expressioni

Gene expression databases

BgeeiENSBTAG00000012012.

Interactioni

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000015944.

Structurei

Secondary structure

1134
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi9 – 12Combined sources4
Helixi14 – 17Combined sources4
Beta strandi21 – 24Combined sources4
Beta strandi25 – 30Combined sources6
Beta strandi33 – 36Combined sources4
Turni38 – 43Combined sources6
Helixi49 – 54Combined sources6
Beta strandi56 – 58Combined sources3
Helixi60 – 65Combined sources6
Helixi70 – 76Combined sources7
Turni77 – 79Combined sources3
Beta strandi80 – 84Combined sources5
Helixi86 – 91Combined sources6

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1CYOX-ray1.50A6-98[»]
1EHBX-ray1.90A8-89[»]
1ES1X-ray2.10A8-89[»]
1F03NMR-A8-89[»]
1F04NMR-A8-89[»]
1HKONMR-A2-105[»]
1I5UNMR-A8-89[»]
1J0QNMR-A8-89[»]
1LQXX-ray1.80A8-89[»]
1LR6X-ray1.90A8-89[»]
1M20X-ray1.80A8-89[»]
1M2IX-ray1.80A8-89[»]
1M2MX-ray1.80A8-89[»]
1M59X-ray1.90A8-89[»]
1NX7NMR-A8-89[»]
1SH4NMR-A8-89[»]
1U9MX-ray2.00A/B/C/D/E/F8-89[»]
1U9UX-ray1.86A8-89[»]
3OZZX-ray1.70B8-89[»]
4HINX-ray2.40A/B/C/D8-89[»]
ProteinModelPortaliP00171.
SMRiP00171.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP00171.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini9 – 85Cytochrome b5 heme-bindingPROSITE-ProRule annotationAdd BLAST77

Sequence similaritiesi

Belongs to the cytochrome b5 family.Curated
Contains 1 cytochrome b5 heme-binding domain.PROSITE-ProRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG0537. Eukaryota.
COG5274. LUCA.
GeneTreeiENSGT00510000046507.
HOGENOMiHOG000039853.
HOVERGENiHBG002653.
InParanoidiP00171.
OMAiFKSTWII.
OrthoDBiEOG091G17G8.
TreeFamiTF314537.

Family and domain databases

Gene3Di3.10.120.10. 1 hit.
InterProiIPR001199. Cyt_B5-like_heme/steroid-bd.
IPR018506. Cyt_B5_heme-BS.
[Graphical view]
PfamiPF00173. Cyt-b5. 1 hit.
[Graphical view]
PRINTSiPR00363. CYTOCHROMEB5.
SMARTiSM01117. Cyt-b5. 1 hit.
[Graphical view]
SUPFAMiSSF55856. SSF55856. 1 hit.
PROSITEiPS00191. CYTOCHROME_B5_1. 1 hit.
PS50255. CYTOCHROME_B5_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P00171-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAEESSKAVK YYTLEEIQKH NNSKSTWLIL HYKVYDLTKF LEEHPGGEEV
60 70 80 90 100
LREQAGGDAT ENFEDVGHST DARELSKTFI IGELHPDDRS KITKPSESII
110 120 130
TTIDSNPSWW TNWLIPAISA LFVALIYHLY TSEN
Length:134
Mass (Da):15,329
Last modified:January 23, 2007 - v3
Checksum:i7B7B605158D97525
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti2 – 5AEES → ZSZZBA AA sequence (PubMed:4810060).Curated4
Sequence conflicti16 – 18EIQ → QIE AA sequence (PubMed:5391285).Curated3
Sequence conflicti18Q → E AA sequence (PubMed:5272324).Curated1
Sequence conflicti62N → D AA sequence (PubMed:5391285).Curated1
Sequence conflicti62N → D AA sequence (PubMed:5272324).Curated1
Sequence conflicti134N → D AA sequence (PubMed:4810060).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X13617 mRNA. Translation: CAA31949.1.
M63328, M63326, M63327 Genomic DNA. Translation: AAC14455.1. Sequence problems.
L22966 Genomic DNA. No translation available.
BC108113 mRNA. Translation: AAI08114.1.
PIRiA47215. CBBO5.
RefSeqiNP_776458.1. NM_174033.3.
UniGeneiBt.65097.

Genome annotation databases

EnsembliENSBTAT00000015944; ENSBTAP00000015944; ENSBTAG00000012012.
GeneIDi281110.
KEGGibta:281110.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X13617 mRNA. Translation: CAA31949.1.
M63328, M63326, M63327 Genomic DNA. Translation: AAC14455.1. Sequence problems.
L22966 Genomic DNA. No translation available.
BC108113 mRNA. Translation: AAI08114.1.
PIRiA47215. CBBO5.
RefSeqiNP_776458.1. NM_174033.3.
UniGeneiBt.65097.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1CYOX-ray1.50A6-98[»]
1EHBX-ray1.90A8-89[»]
1ES1X-ray2.10A8-89[»]
1F03NMR-A8-89[»]
1F04NMR-A8-89[»]
1HKONMR-A2-105[»]
1I5UNMR-A8-89[»]
1J0QNMR-A8-89[»]
1LQXX-ray1.80A8-89[»]
1LR6X-ray1.90A8-89[»]
1M20X-ray1.80A8-89[»]
1M2IX-ray1.80A8-89[»]
1M2MX-ray1.80A8-89[»]
1M59X-ray1.90A8-89[»]
1NX7NMR-A8-89[»]
1SH4NMR-A8-89[»]
1U9MX-ray2.00A/B/C/D/E/F8-89[»]
1U9UX-ray1.86A8-89[»]
3OZZX-ray1.70B8-89[»]
4HINX-ray2.40A/B/C/D8-89[»]
ProteinModelPortaliP00171.
SMRiP00171.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000015944.

Proteomic databases

PaxDbiP00171.
PeptideAtlasiP00171.
PRIDEiP00171.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSBTAT00000015944; ENSBTAP00000015944; ENSBTAG00000012012.
GeneIDi281110.
KEGGibta:281110.

Organism-specific databases

CTDi1528.

Phylogenomic databases

eggNOGiKOG0537. Eukaryota.
COG5274. LUCA.
GeneTreeiENSGT00510000046507.
HOGENOMiHOG000039853.
HOVERGENiHBG002653.
InParanoidiP00171.
OMAiFKSTWII.
OrthoDBiEOG091G17G8.
TreeFamiTF314537.

Enzyme and pathway databases

ReactomeiR-BTA-196836. Vitamin C (ascorbate) metabolism.

Miscellaneous databases

EvolutionaryTraceiP00171.

Gene expression databases

BgeeiENSBTAG00000012012.

Family and domain databases

Gene3Di3.10.120.10. 1 hit.
InterProiIPR001199. Cyt_B5-like_heme/steroid-bd.
IPR018506. Cyt_B5_heme-BS.
[Graphical view]
PfamiPF00173. Cyt-b5. 1 hit.
[Graphical view]
PRINTSiPR00363. CYTOCHROMEB5.
SMARTiSM01117. Cyt-b5. 1 hit.
[Graphical view]
SUPFAMiSSF55856. SSF55856. 1 hit.
PROSITEiPS00191. CYTOCHROME_B5_1. 1 hit.
PS50255. CYTOCHROME_B5_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCYB5_BOVIN
AccessioniPrimary (citable) accession number: P00171
Secondary accession number(s): Q27947, Q28837, Q32PH5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: January 23, 2007
Last modified: November 2, 2016
This is version 158 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.