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P00169

- CYB5_RABIT

UniProt

P00169 - CYB5_RABIT

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Protein

Cytochrome b5

Gene

CYB5A

Organism
Oryctolagus cuniculus (Rabbit)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Cytochrome b5 is a membrane bound hemoprotein which function as an electron carrier for several membrane bound oxygenases.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi44 – 441Iron (heme axial ligand)
Metal bindingi68 – 681Iron (heme axial ligand)

GO - Molecular functioni

  1. heme binding Source: InterPro
  2. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. oxidation-reduction process Source: UniProtKB-KW
Complete GO annotation...

Keywords - Biological processi

Electron transport, Transport

Keywords - Ligandi

Heme, Iron, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Cytochrome b5
Gene namesi
Name:CYB5A
Synonyms:CYB5
OrganismiOryctolagus cuniculus (Rabbit)
Taxonomic identifieri9986 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresLagomorphaLeporidaeOryctolagus
ProteomesiUP000001811: Chromosome 9

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei109 – 13123HelicalSequence AnalysisAdd
BLAST

GO - Cellular componenti

  1. endoplasmic reticulum Source: UniProtKB-KW
  2. extracellular vesicular exosome Source: Ensembl
  3. integral component of membrane Source: UniProtKB-KW
  4. mitochondrion Source: Ensembl
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane, Microsome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed1 Publication
Chaini2 – 134133Cytochrome b5PRO_0000166013Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylalanine1 Publication
Modified residuei7 – 71N6-acetyllysineBy similarity
Modified residuei10 – 101N6-acetyllysineBy similarity
Modified residuei19 – 191N6-acetyllysineBy similarity

Keywords - PTMi

Acetylation

Proteomic databases

PRIDEiP00169.

Interactioni

Protein-protein interaction databases

STRINGi9986.ENSOCUP00000013951.

Structurei

Secondary structure

1
134
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi10 – 123Combined sources
Helixi14 – 174Combined sources
Turni18 – 203Combined sources
Beta strandi22 – 254Combined sources
Beta strandi27 – 3610Combined sources
Helixi38 – 403Combined sources
Turni41 – 433Combined sources
Beta strandi44 – 463Combined sources
Helixi49 – 535Combined sources
Turni54 – 563Combined sources
Helixi60 – 667Combined sources
Helixi70 – 7910Combined sources
Beta strandi80 – 845Combined sources
Helixi86 – 883Combined sources
Turni89 – 913Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1DO9NMR-A6-99[»]
2M33NMR-A1-104[»]
ProteinModelPortaliP00169.
SMRiP00169. Positions 6-99.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP00169.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini9 – 8577Cytochrome b5 heme-bindingPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the cytochrome b5 family.Curated
Contains 1 cytochrome b5 heme-binding domain.PROSITE-ProRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG5274.
GeneTreeiENSGT00510000046507.
HOGENOMiHOG000039853.
HOVERGENiHBG002653.
InParanoidiP00169.
OMAiMAEQSDK.
OrthoDBiEOG78H3W2.
TreeFamiTF314537.

Family and domain databases

Gene3Di3.10.120.10. 1 hit.
InterProiIPR001199. Cyt_B5-like_heme/steroid-bd.
IPR018506. Cyt_B5_heme-BS.
[Graphical view]
PfamiPF00173. Cyt-b5. 1 hit.
[Graphical view]
PRINTSiPR00363. CYTOCHROMEB5.
SUPFAMiSSF55856. SSF55856. 1 hit.
PROSITEiPS00191. CYTOCHROME_B5_1. 1 hit.
PS50255. CYTOCHROME_B5_2. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: P00169-1) [UniParc]FASTAAdd to Basket

Also known as: Liver, Membrane-bound

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAAQSDKDVK YYTLEEIKKH NHSKSTWLIL HHKVYDLTKF LEEHPGGEEV
60 70 80 90 100
LREQAGGDAT ENFEDVGHST DARELSKTFI IGELHPDDRS KLSKPMETLI
110 120 130
TTVDSNSSWW TNWVIPAISA LIVALMYRLY MADD
Length:134
Mass (Da):15,349
Last modified:January 23, 2007 - v4
Checksum:iB748AE9D32FA5E46
GO
Isoform 2 (identifier: P00169-2) [UniParc]FASTAAdd to Basket

Also known as: Erythrocyte, Cytoplasmic

The sequence of this isoform differs from the canonical sequence as follows:
     98-98: T → P
     99-134: Missing.

Show »
Length:98
Mass (Da):11,232
Checksum:iC3D197FD9C921C1D
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti62 – 621N → D AA sequence (PubMed:5709273)Curated
Sequence conflicti62 – 621N → D AA sequence (PubMed:5506260)Curated
Sequence conflicti104 – 1041D → N AA sequence (PubMed:500581)Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei98 – 981T → P in isoform 2. 1 PublicationVSP_001244
Alternative sequencei99 – 13436Missing in isoform 2. 1 PublicationVSP_001245Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M24844 mRNA. Translation: AAB03878.1.
D10901 mRNA. Translation: BAA01712.1.
PIRiJN0316.
S03373. CBRB5.
RefSeqiNP_001164734.1. NM_001171263.1. [P00169-2]
NP_001164735.1. NM_001171264.1. [P00169-1]
UniGeneiOcu.2112.
Ocu.6903.

Genome annotation databases

EnsembliENSOCUT00000016229; ENSOCUP00000013951; ENSOCUG00000016235. [P00169-1]
GeneIDi100328912.
100328915.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M24844 mRNA. Translation: AAB03878.1 .
D10901 mRNA. Translation: BAA01712.1 .
PIRi JN0316.
S03373. CBRB5.
RefSeqi NP_001164734.1. NM_001171263.1. [P00169-2 ]
NP_001164735.1. NM_001171264.1. [P00169-1 ]
UniGenei Ocu.2112.
Ocu.6903.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1DO9 NMR - A 6-99 [» ]
2M33 NMR - A 1-104 [» ]
ProteinModelPortali P00169.
SMRi P00169. Positions 6-99.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 9986.ENSOCUP00000013951.

Proteomic databases

PRIDEi P00169.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSOCUT00000016229 ; ENSOCUP00000013951 ; ENSOCUG00000016235 . [P00169-1 ]
GeneIDi 100328912.
100328915.

Phylogenomic databases

eggNOGi COG5274.
GeneTreei ENSGT00510000046507.
HOGENOMi HOG000039853.
HOVERGENi HBG002653.
InParanoidi P00169.
OMAi MAEQSDK.
OrthoDBi EOG78H3W2.
TreeFami TF314537.

Miscellaneous databases

EvolutionaryTracei P00169.

Family and domain databases

Gene3Di 3.10.120.10. 1 hit.
InterProi IPR001199. Cyt_B5-like_heme/steroid-bd.
IPR018506. Cyt_B5_heme-BS.
[Graphical view ]
Pfami PF00173. Cyt-b5. 1 hit.
[Graphical view ]
PRINTSi PR00363. CYTOCHROMEB5.
SUPFAMi SSF55856. SSF55856. 1 hit.
PROSITEi PS00191. CYTOCHROME_B5_1. 1 hit.
PS50255. CYTOCHROME_B5_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "The nucleotide sequence of rabbit liver cytochrome b5 mRNA."
    Dariush N., Fisher C.W., Steggles A.W.
    Protein Seq. Data Anal. 1:351-353(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
  2. "Molecular cloning of rabbit cytochrome b5 genes: evidence for the occurrence of two separate genes encoding the soluble and microsomal forms."
    Takamatsu H., Kozutsumi Y., Suzuki A., Kawasaki T.
    Biochem. Biophys. Res. Commun. 185:845-851(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2).
    Strain: New Zealand white.
  3. "Primary structure of rabbit liver cytochrome b5."
    Tsugita A., Kobayashi M., Kajihara T., Hagihara B.
    J. Biochem. 64:727-730(1968) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 9-46 AND 50-91.
  4. "Comparative study of the primary structures of cytochrome b5 from four species."
    Tsugita A., Kobayashi M., Tani S., Kyo S., Rashid M.A., Yoshida Y., Kajihara T., Hagihara B.
    Proc. Natl. Acad. Sci. U.S.A. 67:442-447(1970) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 7-8 AND 47-49.
  5. "Amino acid sequence of rabbit liver microsomal cytochrome b5."
    Ozols J.
    J. Biol. Chem. 245:4863-4874(1970) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 5-98.
  6. "Primary structure of the membrane-binding segment of rabbit cytochrome b5."
    Kondo K., Tajima S., Sato R., Narita K.
    J. Biochem. 86:1119-1128(1979) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 92-134.
  7. "Amino acid sequence of the membranous segment of rabbit liver cytochrome b5. Methodology for separation of hydrophobic peptides."
    Takagaki Y., Gerber G.E., Nihei K., Khorana H.G.
    J. Biol. Chem. 255:1536-1541(1980) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 99-134.
  8. "Structure of cytochrome b5 and its topology in the microsomal membrane."
    Ozols J.
    Biochim. Biophys. Acta 997:121-130(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 2-11.
  9. "Solution structure of oxidized microsomal rabbit cytochrome b5 factors determining the heterogeneous binding of the heme."
    Banci L., Bertini I., Rosato A., Scacchieri S.
    Eur. J. Biochem. 267:755-766(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: STRUCTURE BY NMR OF 7-100.

Entry informationi

Entry nameiCYB5_RABIT
AccessioniPrimary (citable) accession number: P00169
Secondary accession number(s): Q28726
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: January 23, 2007
Last modified: November 26, 2014
This is version 120 of the entry and version 4 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3