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Protein

Cytochrome b5

Gene

CYB5A

Organism
Oryctolagus cuniculus (Rabbit)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Cytochrome b5 is a membrane bound hemoprotein which function as an electron carrier for several membrane bound oxygenases.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi44Iron (heme axial ligand)1
Metal bindingi68Iron (heme axial ligand)1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Electron transport, Transport

Keywords - Ligandi

Heme, Iron, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Cytochrome b5
Gene namesi
Name:CYB5A
Synonyms:CYB5
OrganismiOryctolagus cuniculus (Rabbit)
Taxonomic identifieri9986 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresLagomorphaLeporidaeOryctolagus
Proteomesi
  • UP000001811 Componenti: Chromosome 9

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei109 – 131HelicalSequence analysisAdd BLAST23

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane, Microsome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved1 Publication
ChainiPRO_00001660132 – 134Cytochrome b5Add BLAST133

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylalanine1 Publication1
Modified residuei7N6-acetyllysineBy similarity1
Modified residuei10N6-acetyllysineBy similarity1
Modified residuei19N6-acetyllysineBy similarity1

Keywords - PTMi

Acetylation

Proteomic databases

PRIDEiP00169.

Interactioni

Protein-protein interaction databases

STRINGi9986.ENSOCUP00000013951.

Structurei

Secondary structure

1134
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi10 – 12Combined sources3
Helixi14 – 17Combined sources4
Turni18 – 20Combined sources3
Beta strandi22 – 25Combined sources4
Beta strandi27 – 36Combined sources10
Helixi38 – 40Combined sources3
Turni41 – 43Combined sources3
Beta strandi44 – 46Combined sources3
Helixi49 – 53Combined sources5
Turni54 – 56Combined sources3
Helixi60 – 66Combined sources7
Helixi70 – 79Combined sources10
Beta strandi80 – 84Combined sources5
Helixi86 – 88Combined sources3
Turni89 – 91Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1DO9NMR-A6-99[»]
2M33NMR-A1-134[»]
ProteinModelPortaliP00169.
SMRiP00169.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP00169.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini9 – 85Cytochrome b5 heme-bindingPROSITE-ProRule annotationAdd BLAST77

Sequence similaritiesi

Belongs to the cytochrome b5 family.Curated
Contains 1 cytochrome b5 heme-binding domain.PROSITE-ProRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG0537. Eukaryota.
COG5274. LUCA.
GeneTreeiENSGT00510000046507.
HOGENOMiHOG000039853.
HOVERGENiHBG002653.
InParanoidiP00169.
OMAiFKSTWII.
OrthoDBiEOG091G17G8.
TreeFamiTF314537.

Family and domain databases

Gene3Di3.10.120.10. 1 hit.
InterProiIPR001199. Cyt_B5-like_heme/steroid-bd.
IPR018506. Cyt_B5_heme-BS.
[Graphical view]
PfamiPF00173. Cyt-b5. 1 hit.
[Graphical view]
PRINTSiPR00363. CYTOCHROMEB5.
SMARTiSM01117. Cyt-b5. 1 hit.
[Graphical view]
SUPFAMiSSF55856. SSF55856. 1 hit.
PROSITEiPS00191. CYTOCHROME_B5_1. 1 hit.
PS50255. CYTOCHROME_B5_2. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: P00169-1) [UniParc]FASTAAdd to basket
Also known as: Liver, Membrane-bound

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAAQSDKDVK YYTLEEIKKH NHSKSTWLIL HHKVYDLTKF LEEHPGGEEV
60 70 80 90 100
LREQAGGDAT ENFEDVGHST DARELSKTFI IGELHPDDRS KLSKPMETLI
110 120 130
TTVDSNSSWW TNWVIPAISA LIVALMYRLY MADD
Length:134
Mass (Da):15,349
Last modified:January 23, 2007 - v4
Checksum:iB748AE9D32FA5E46
GO
Isoform 2 (identifier: P00169-2) [UniParc]FASTAAdd to basket
Also known as: Erythrocyte, Cytoplasmic

The sequence of this isoform differs from the canonical sequence as follows:
     98-98: T → P
     99-134: Missing.

Show »
Length:98
Mass (Da):11,232
Checksum:iC3D197FD9C921C1D
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti62N → D AA sequence (PubMed:5709273).Curated1
Sequence conflicti62N → D AA sequence (PubMed:5506260).Curated1
Sequence conflicti104D → N AA sequence (PubMed:500581).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_00124498T → P in isoform 2. 1 Publication1
Alternative sequenceiVSP_00124599 – 134Missing in isoform 2. 1 PublicationAdd BLAST36

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M24844 mRNA. Translation: AAB03878.1.
D10901 mRNA. Translation: BAA01712.1.
PIRiJN0316.
S03373. CBRB5.
RefSeqiNP_001164734.1. NM_001171263.1. [P00169-2]
NP_001164735.1. NM_001171264.1. [P00169-1]
XP_017199148.1. XM_017343659.1. [P00169-1]
UniGeneiOcu.2112.
Ocu.6903.

Genome annotation databases

EnsembliENSOCUT00000016229; ENSOCUP00000013951; ENSOCUG00000016235. [P00169-1]
GeneIDi100328912.
100328915.
KEGGiocu:100328912.
ocu:100328915.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M24844 mRNA. Translation: AAB03878.1.
D10901 mRNA. Translation: BAA01712.1.
PIRiJN0316.
S03373. CBRB5.
RefSeqiNP_001164734.1. NM_001171263.1. [P00169-2]
NP_001164735.1. NM_001171264.1. [P00169-1]
XP_017199148.1. XM_017343659.1. [P00169-1]
UniGeneiOcu.2112.
Ocu.6903.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1DO9NMR-A6-99[»]
2M33NMR-A1-134[»]
ProteinModelPortaliP00169.
SMRiP00169.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9986.ENSOCUP00000013951.

Proteomic databases

PRIDEiP00169.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSOCUT00000016229; ENSOCUP00000013951; ENSOCUG00000016235. [P00169-1]
GeneIDi100328912.
100328915.
KEGGiocu:100328912.
ocu:100328915.

Phylogenomic databases

eggNOGiKOG0537. Eukaryota.
COG5274. LUCA.
GeneTreeiENSGT00510000046507.
HOGENOMiHOG000039853.
HOVERGENiHBG002653.
InParanoidiP00169.
OMAiFKSTWII.
OrthoDBiEOG091G17G8.
TreeFamiTF314537.

Miscellaneous databases

EvolutionaryTraceiP00169.

Family and domain databases

Gene3Di3.10.120.10. 1 hit.
InterProiIPR001199. Cyt_B5-like_heme/steroid-bd.
IPR018506. Cyt_B5_heme-BS.
[Graphical view]
PfamiPF00173. Cyt-b5. 1 hit.
[Graphical view]
PRINTSiPR00363. CYTOCHROMEB5.
SMARTiSM01117. Cyt-b5. 1 hit.
[Graphical view]
SUPFAMiSSF55856. SSF55856. 1 hit.
PROSITEiPS00191. CYTOCHROME_B5_1. 1 hit.
PS50255. CYTOCHROME_B5_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCYB5_RABIT
AccessioniPrimary (citable) accession number: P00169
Secondary accession number(s): Q28726
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: January 23, 2007
Last modified: November 2, 2016
This is version 136 of the entry and version 4 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.