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P00169 (CYB5_RABIT) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 117. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Cytochrome b5
Gene names
Name:CYB5A
Synonyms:CYB5
OrganismOryctolagus cuniculus (Rabbit) [Reference proteome]
Taxonomic identifier9986 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresLagomorphaLeporidaeOryctolagus

Protein attributes

Sequence length134 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Cytochrome b5 is a membrane bound hemoprotein which function as an electron carrier for several membrane bound oxygenases.

Subcellular location

Endoplasmic reticulum membrane; Single-pass membrane protein; Cytoplasmic side. Microsome membrane; Single-pass membrane protein; Cytoplasmic side.

Sequence similarities

Belongs to the cytochrome b5 family.

Contains 1 cytochrome b5 heme-binding domain.

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: P00169-1)

Also known as: Liver; Membrane-bound;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: P00169-2)

Also known as: Erythrocyte; Cytoplasmic;

The sequence of this isoform differs from the canonical sequence as follows:
     98-98: T → P
     99-134: Missing.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed Ref.8
Chain2 – 134133Cytochrome b5
PRO_0000166013

Regions

Transmembrane109 – 13123Helical; Potential
Domain9 – 8577Cytochrome b5 heme-binding

Sites

Metal binding441Iron (heme axial ligand)
Metal binding681Iron (heme axial ligand)

Amino acid modifications

Modified residue21N-acetylalanine Ref.8
Modified residue71N6-acetyllysine By similarity
Modified residue101N6-acetyllysine By similarity
Modified residue191N6-acetyllysine By similarity

Natural variations

Alternative sequence981T → P in isoform 2.
VSP_001244
Alternative sequence99 – 13436Missing in isoform 2.
VSP_001245

Experimental info

Sequence conflict621N → D AA sequence Ref.3
Sequence conflict621N → D AA sequence Ref.5
Sequence conflict1041D → N AA sequence Ref.6

Secondary structure

......................... 134
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 (Liver) (Membrane-bound) [UniParc].

Last modified January 23, 2007. Version 4.
Checksum: B748AE9D32FA5E46

FASTA13415,349
        10         20         30         40         50         60 
MAAQSDKDVK YYTLEEIKKH NHSKSTWLIL HHKVYDLTKF LEEHPGGEEV LREQAGGDAT 

        70         80         90        100        110        120 
ENFEDVGHST DARELSKTFI IGELHPDDRS KLSKPMETLI TTVDSNSSWW TNWVIPAISA 

       130 
LIVALMYRLY MADD 

« Hide

Isoform 2 (Erythrocyte) (Cytoplasmic) [UniParc].

Checksum: C3D197FD9C921C1D
Show »

FASTA9811,232

References

[1]"The nucleotide sequence of rabbit liver cytochrome b5 mRNA."
Dariush N., Fisher C.W., Steggles A.W.
Protein Seq. Data Anal. 1:351-353(1988) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
[2]"Molecular cloning of rabbit cytochrome b5 genes: evidence for the occurrence of two separate genes encoding the soluble and microsomal forms."
Takamatsu H., Kozutsumi Y., Suzuki A., Kawasaki T.
Biochem. Biophys. Res. Commun. 185:845-851(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2).
Strain: New Zealand white.
[3]"Primary structure of rabbit liver cytochrome b5."
Tsugita A., Kobayashi M., Kajihara T., Hagihara B.
J. Biochem. 64:727-730(1968) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 9-46 AND 50-91.
[4]"Comparative study of the primary structures of cytochrome b5 from four species."
Tsugita A., Kobayashi M., Tani S., Kyo S., Rashid M.A., Yoshida Y., Kajihara T., Hagihara B.
Proc. Natl. Acad. Sci. U.S.A. 67:442-447(1970) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 7-8 AND 47-49.
[5]"Amino acid sequence of rabbit liver microsomal cytochrome b5."
Ozols J.
J. Biol. Chem. 245:4863-4874(1970) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 5-98.
[6]"Primary structure of the membrane-binding segment of rabbit cytochrome b5."
Kondo K., Tajima S., Sato R., Narita K.
J. Biochem. 86:1119-1128(1979) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 92-134.
[7]"Amino acid sequence of the membranous segment of rabbit liver cytochrome b5. Methodology for separation of hydrophobic peptides."
Takagaki Y., Gerber G.E., Nihei K., Khorana H.G.
J. Biol. Chem. 255:1536-1541(1980) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 99-134.
[8]"Structure of cytochrome b5 and its topology in the microsomal membrane."
Ozols J.
Biochim. Biophys. Acta 997:121-130(1989) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 2-11.
[9]"Solution structure of oxidized microsomal rabbit cytochrome b5 factors determining the heterogeneous binding of the heme."
Banci L., Bertini I., Rosato A., Scacchieri S.
Eur. J. Biochem. 267:755-766(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: STRUCTURE BY NMR OF 7-100.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M24844 mRNA. Translation: AAB03878.1.
D10901 mRNA. Translation: BAA01712.1.
PIRJN0316.
CBRB5. S03373.
RefSeqNP_001164734.1. NM_001171263.1. [P00169-2]
NP_001164735.1. NM_001171264.1. [P00169-1]
UniGeneOcu.2112.
Ocu.6903.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1DO9NMR-A6-99[»]
2M33NMR-A1-104[»]
ProteinModelPortalP00169.
SMRP00169. Positions 6-99.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING9986.ENSOCUP00000013951.

Proteomic databases

PRIDEP00169.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSOCUT00000016229; ENSOCUP00000013951; ENSOCUG00000016235. [P00169-1]
GeneID100328912.
100328915.

Phylogenomic databases

eggNOGCOG5274.
GeneTreeENSGT00510000046507.
HOGENOMHOG000039853.
HOVERGENHBG002653.
OMAMAEQSDK.
OrthoDBEOG78H3W2.
TreeFamTF314537.

Family and domain databases

Gene3D3.10.120.10. 1 hit.
InterProIPR001199. Cyt_B5-like_heme/steroid-bd.
IPR018506. Cyt_B5_heme-BS.
[Graphical view]
PfamPF00173. Cyt-b5. 1 hit.
[Graphical view]
PRINTSPR00363. CYTOCHROMEB5.
SUPFAMSSF55856. SSF55856. 1 hit.
PROSITEPS00191. CYTOCHROME_B5_1. 1 hit.
PS50255. CYTOCHROME_B5_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceP00169.

Entry information

Entry nameCYB5_RABIT
AccessionPrimary (citable) accession number: P00169
Secondary accession number(s): Q28726
Entry history
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: January 23, 2007
Last modified: May 14, 2014
This is version 117 of the entry and version 4 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references