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Protein

Cytochrome b5

Gene

CYB5A

Organism
Oryctolagus cuniculus (Rabbit)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Cytochrome b5 is a membrane bound hemoprotein which function as an electron carrier for several membrane bound oxygenases.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi44Iron (heme axial ligand)1
Metal bindingi68Iron (heme axial ligand)1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Biological processElectron transport, Transport
LigandHeme, Iron, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Cytochrome b5
Gene namesi
Name:CYB5A
Synonyms:CYB5
OrganismiOryctolagus cuniculus (Rabbit)
Taxonomic identifieri9986 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresLagomorphaLeporidaeOryctolagus
Proteomesi

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei109 – 131HelicalSequence analysisAdd BLAST23

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane, Microsome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved1 Publication
ChainiPRO_00001660132 – 134Cytochrome b5Add BLAST133

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylalanine1 Publication1
Modified residuei7N6-acetyllysineBy similarity1
Modified residuei10N6-acetyllysineBy similarity1
Modified residuei19N6-acetyllysineBy similarity1

Keywords - PTMi

Acetylation

Proteomic databases

PRIDEiP00169

PTM databases

iPTMnetiP00169

Expressioni

Gene expression databases

BgeeiENSOCUG00000016235

Interactioni

GO - Molecular functioni

Protein-protein interaction databases

STRINGi9986.ENSOCUP00000013951

Structurei

Secondary structure

1134
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi10 – 12Combined sources3
Helixi14 – 17Combined sources4
Turni18 – 20Combined sources3
Beta strandi22 – 25Combined sources4
Beta strandi27 – 36Combined sources10
Helixi38 – 40Combined sources3
Turni41 – 43Combined sources3
Beta strandi44 – 46Combined sources3
Helixi49 – 53Combined sources5
Turni54 – 56Combined sources3
Helixi60 – 66Combined sources7
Helixi70 – 79Combined sources10
Beta strandi80 – 84Combined sources5
Helixi86 – 88Combined sources3
Turni89 – 91Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1DO9NMR-A6-99[»]
2M33NMR-A1-134[»]
ProteinModelPortaliP00169
SMRiP00169
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP00169

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini9 – 85Cytochrome b5 heme-bindingPROSITE-ProRule annotationAdd BLAST77

Sequence similaritiesi

Belongs to the cytochrome b5 family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG0537 Eukaryota
COG5274 LUCA
GeneTreeiENSGT00510000046507
HOGENOMiHOG000039853
HOVERGENiHBG002653
InParanoidiP00169
OMAiFKSTWII
OrthoDBiEOG091G17G8
TreeFamiTF314537

Family and domain databases

Gene3Di3.10.120.10, 1 hit
InterProiView protein in InterPro
IPR001199 Cyt_B5-like_heme/steroid-bd
IPR036400 Cyt_B5-like_heme/steroid_sf
IPR018506 Cyt_B5_heme-BS
PfamiView protein in Pfam
PF00173 Cyt-b5, 1 hit
PRINTSiPR00363 CYTOCHROMEB5
SMARTiView protein in SMART
SM01117 Cyt-b5, 1 hit
SUPFAMiSSF55856 SSF55856, 1 hit
PROSITEiView protein in PROSITE
PS00191 CYTOCHROME_B5_1, 1 hit
PS50255 CYTOCHROME_B5_2, 1 hit

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: P00169-1) [UniParc]FASTAAdd to basket
Also known as: Liver, Membrane-bound

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAAQSDKDVK YYTLEEIKKH NHSKSTWLIL HHKVYDLTKF LEEHPGGEEV
60 70 80 90 100
LREQAGGDAT ENFEDVGHST DARELSKTFI IGELHPDDRS KLSKPMETLI
110 120 130
TTVDSNSSWW TNWVIPAISA LIVALMYRLY MADD
Length:134
Mass (Da):15,349
Last modified:January 23, 2007 - v4
Checksum:iB748AE9D32FA5E46
GO
Isoform 2 (identifier: P00169-2) [UniParc]FASTAAdd to basket
Also known as: Erythrocyte, Cytoplasmic

The sequence of this isoform differs from the canonical sequence as follows:
     98-98: T → P
     99-134: Missing.

Show »
Length:98
Mass (Da):11,232
Checksum:iC3D197FD9C921C1D
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti62N → D AA sequence (PubMed:5709273).Curated1
Sequence conflicti62N → D AA sequence (PubMed:5506260).Curated1
Sequence conflicti104D → N AA sequence (PubMed:500581).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_00124498T → P in isoform 2. 1 Publication1
Alternative sequenceiVSP_00124599 – 134Missing in isoform 2. 1 PublicationAdd BLAST36

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M24844 mRNA Translation: AAB03878.1
D10901 mRNA Translation: BAA01712.1
PIRiJN0316
S03373 CBRB5
RefSeqiNP_001164734.1, NM_001171263.1 [P00169-2]
NP_001164735.1, NM_001171264.1 [P00169-1]
XP_017199148.1, XM_017343659.1 [P00169-1]
UniGeneiOcu.2112
Ocu.6903

Genome annotation databases

EnsembliENSOCUT00000016229; ENSOCUP00000013951; ENSOCUG00000016235 [P00169-1]
GeneIDi100328912
100328915
KEGGiocu:100328912
ocu:100328915

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiCYB5_RABIT
AccessioniPrimary (citable) accession number: P00169
Secondary accession number(s): Q28726
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: January 23, 2007
Last modified: May 23, 2018
This is version 143 of the entry and version 4 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

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