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P00169

- CYB5_RABIT

UniProt

P00169 - CYB5_RABIT

Protein

Cytochrome b5

Gene

CYB5A

Organism
Oryctolagus cuniculus (Rabbit)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
  1. Functioni

    Cytochrome b5 is a membrane bound hemoprotein which function as an electron carrier for several membrane bound oxygenases.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi44 – 441Iron (heme axial ligand)
    Metal bindingi68 – 681Iron (heme axial ligand)

    GO - Molecular functioni

    1. heme binding Source: InterPro
    2. metal ion binding Source: UniProtKB-KW

    GO - Biological processi

    1. oxidation-reduction process Source: UniProtKB-KW

    Keywords - Biological processi

    Electron transport, Transport

    Keywords - Ligandi

    Heme, Iron, Metal-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Cytochrome b5
    Gene namesi
    Name:CYB5A
    Synonyms:CYB5
    OrganismiOryctolagus cuniculus (Rabbit)
    Taxonomic identifieri9986 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresLagomorphaLeporidaeOryctolagus
    ProteomesiUP000001811: Chromosome 9

    Subcellular locationi

    GO - Cellular componenti

    1. endoplasmic reticulum membrane Source: UniProtKB-SubCell
    2. integral component of membrane Source: UniProtKB-KW
    3. mitochondrion Source: Ensembl

    Keywords - Cellular componenti

    Endoplasmic reticulum, Membrane, Microsome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11Removed1 Publication
    Chaini2 – 134133Cytochrome b5PRO_0000166013Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei2 – 21N-acetylalanine1 Publication
    Modified residuei7 – 71N6-acetyllysineBy similarity
    Modified residuei10 – 101N6-acetyllysineBy similarity
    Modified residuei19 – 191N6-acetyllysineBy similarity

    Keywords - PTMi

    Acetylation

    Proteomic databases

    PRIDEiP00169.

    Interactioni

    Protein-protein interaction databases

    STRINGi9986.ENSOCUP00000013951.

    Structurei

    Secondary structure

    1
    134
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi10 – 123
    Helixi14 – 174
    Turni18 – 203
    Beta strandi22 – 254
    Beta strandi27 – 3610
    Helixi38 – 403
    Turni41 – 433
    Beta strandi44 – 463
    Helixi49 – 535
    Turni54 – 563
    Helixi60 – 667
    Helixi70 – 7910
    Beta strandi80 – 845
    Helixi86 – 883
    Turni89 – 913

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1DO9NMR-A6-99[»]
    2M33NMR-A1-104[»]
    ProteinModelPortaliP00169.
    SMRiP00169. Positions 6-99.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP00169.

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei109 – 13123HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini9 – 8577Cytochrome b5 heme-bindingPROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the cytochrome b5 family.Curated
    Contains 1 cytochrome b5 heme-binding domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiCOG5274.
    GeneTreeiENSGT00510000046507.
    HOGENOMiHOG000039853.
    HOVERGENiHBG002653.
    OMAiMAEQSDK.
    OrthoDBiEOG78H3W2.
    TreeFamiTF314537.

    Family and domain databases

    Gene3Di3.10.120.10. 1 hit.
    InterProiIPR001199. Cyt_B5-like_heme/steroid-bd.
    IPR018506. Cyt_B5_heme-BS.
    [Graphical view]
    PfamiPF00173. Cyt-b5. 1 hit.
    [Graphical view]
    PRINTSiPR00363. CYTOCHROMEB5.
    SUPFAMiSSF55856. SSF55856. 1 hit.
    PROSITEiPS00191. CYTOCHROME_B5_1. 1 hit.
    PS50255. CYTOCHROME_B5_2. 1 hit.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: P00169-1) [UniParc]FASTAAdd to Basket

    Also known as: Liver, Membrane-bound

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MAAQSDKDVK YYTLEEIKKH NHSKSTWLIL HHKVYDLTKF LEEHPGGEEV    50
    LREQAGGDAT ENFEDVGHST DARELSKTFI IGELHPDDRS KLSKPMETLI 100
    TTVDSNSSWW TNWVIPAISA LIVALMYRLY MADD 134
    Length:134
    Mass (Da):15,349
    Last modified:January 23, 2007 - v4
    Checksum:iB748AE9D32FA5E46
    GO
    Isoform 2 (identifier: P00169-2) [UniParc]FASTAAdd to Basket

    Also known as: Erythrocyte, Cytoplasmic

    The sequence of this isoform differs from the canonical sequence as follows:
         98-98: T → P
         99-134: Missing.

    Show »
    Length:98
    Mass (Da):11,232
    Checksum:iC3D197FD9C921C1D
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti62 – 621N → D AA sequence (PubMed:5709273)Curated
    Sequence conflicti62 – 621N → D AA sequence (PubMed:5506260)Curated
    Sequence conflicti104 – 1041D → N AA sequence (PubMed:500581)Curated

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei98 – 981T → P in isoform 2. 1 PublicationVSP_001244
    Alternative sequencei99 – 13436Missing in isoform 2. 1 PublicationVSP_001245Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M24844 mRNA. Translation: AAB03878.1.
    D10901 mRNA. Translation: BAA01712.1.
    PIRiJN0316.
    S03373. CBRB5.
    RefSeqiNP_001164734.1. NM_001171263.1. [P00169-2]
    NP_001164735.1. NM_001171264.1. [P00169-1]
    UniGeneiOcu.2112.
    Ocu.6903.

    Genome annotation databases

    EnsembliENSOCUT00000016229; ENSOCUP00000013951; ENSOCUG00000016235. [P00169-1]
    GeneIDi100328912.
    100328915.

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M24844 mRNA. Translation: AAB03878.1 .
    D10901 mRNA. Translation: BAA01712.1 .
    PIRi JN0316.
    S03373. CBRB5.
    RefSeqi NP_001164734.1. NM_001171263.1. [P00169-2 ]
    NP_001164735.1. NM_001171264.1. [P00169-1 ]
    UniGenei Ocu.2112.
    Ocu.6903.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1DO9 NMR - A 6-99 [» ]
    2M33 NMR - A 1-104 [» ]
    ProteinModelPortali P00169.
    SMRi P00169. Positions 6-99.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 9986.ENSOCUP00000013951.

    Proteomic databases

    PRIDEi P00169.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSOCUT00000016229 ; ENSOCUP00000013951 ; ENSOCUG00000016235 . [P00169-1 ]
    GeneIDi 100328912.
    100328915.

    Phylogenomic databases

    eggNOGi COG5274.
    GeneTreei ENSGT00510000046507.
    HOGENOMi HOG000039853.
    HOVERGENi HBG002653.
    OMAi MAEQSDK.
    OrthoDBi EOG78H3W2.
    TreeFami TF314537.

    Miscellaneous databases

    EvolutionaryTracei P00169.

    Family and domain databases

    Gene3Di 3.10.120.10. 1 hit.
    InterProi IPR001199. Cyt_B5-like_heme/steroid-bd.
    IPR018506. Cyt_B5_heme-BS.
    [Graphical view ]
    Pfami PF00173. Cyt-b5. 1 hit.
    [Graphical view ]
    PRINTSi PR00363. CYTOCHROMEB5.
    SUPFAMi SSF55856. SSF55856. 1 hit.
    PROSITEi PS00191. CYTOCHROME_B5_1. 1 hit.
    PS50255. CYTOCHROME_B5_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The nucleotide sequence of rabbit liver cytochrome b5 mRNA."
      Dariush N., Fisher C.W., Steggles A.W.
      Protein Seq. Data Anal. 1:351-353(1988) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
    2. "Molecular cloning of rabbit cytochrome b5 genes: evidence for the occurrence of two separate genes encoding the soluble and microsomal forms."
      Takamatsu H., Kozutsumi Y., Suzuki A., Kawasaki T.
      Biochem. Biophys. Res. Commun. 185:845-851(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2).
      Strain: New Zealand white.
    3. "Primary structure of rabbit liver cytochrome b5."
      Tsugita A., Kobayashi M., Kajihara T., Hagihara B.
      J. Biochem. 64:727-730(1968) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 9-46 AND 50-91.
    4. "Comparative study of the primary structures of cytochrome b5 from four species."
      Tsugita A., Kobayashi M., Tani S., Kyo S., Rashid M.A., Yoshida Y., Kajihara T., Hagihara B.
      Proc. Natl. Acad. Sci. U.S.A. 67:442-447(1970) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 7-8 AND 47-49.
    5. "Amino acid sequence of rabbit liver microsomal cytochrome b5."
      Ozols J.
      J. Biol. Chem. 245:4863-4874(1970) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 5-98.
    6. "Primary structure of the membrane-binding segment of rabbit cytochrome b5."
      Kondo K., Tajima S., Sato R., Narita K.
      J. Biochem. 86:1119-1128(1979) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 92-134.
    7. "Amino acid sequence of the membranous segment of rabbit liver cytochrome b5. Methodology for separation of hydrophobic peptides."
      Takagaki Y., Gerber G.E., Nihei K., Khorana H.G.
      J. Biol. Chem. 255:1536-1541(1980) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 99-134.
    8. "Structure of cytochrome b5 and its topology in the microsomal membrane."
      Ozols J.
      Biochim. Biophys. Acta 997:121-130(1989) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 2-11.
    9. "Solution structure of oxidized microsomal rabbit cytochrome b5 factors determining the heterogeneous binding of the heme."
      Banci L., Bertini I., Rosato A., Scacchieri S.
      Eur. J. Biochem. 267:755-766(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: STRUCTURE BY NMR OF 7-100.

    Entry informationi

    Entry nameiCYB5_RABIT
    AccessioniPrimary (citable) accession number: P00169
    Secondary accession number(s): Q28726
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 21, 1986
    Last sequence update: January 23, 2007
    Last modified: October 1, 2014
    This is version 118 of the entry and version 4 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3