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Protein

Cytochrome b5

Gene

CYB5A

Organism
Oryctolagus cuniculus (Rabbit)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Cytochrome b5 is a membrane bound hemoprotein which function as an electron carrier for several membrane bound oxygenases.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi44 – 441Iron (heme axial ligand)
Metal bindingi68 – 681Iron (heme axial ligand)

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Electron transport, Transport

Keywords - Ligandi

Heme, Iron, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Cytochrome b5
Gene namesi
Name:CYB5A
Synonyms:CYB5
OrganismiOryctolagus cuniculus (Rabbit)
Taxonomic identifieri9986 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresLagomorphaLeporidaeOryctolagus
Proteomesi
  • UP000001811 Componenti: Chromosome 9

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei109 – 13123HelicalSequence analysisAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane, Microsome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemoved1 Publication
Chaini2 – 134133Cytochrome b5PRO_0000166013Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylalanine1 Publication
Modified residuei7 – 71N6-acetyllysineBy similarity
Modified residuei10 – 101N6-acetyllysineBy similarity
Modified residuei19 – 191N6-acetyllysineBy similarity

Keywords - PTMi

Acetylation

Proteomic databases

PRIDEiP00169.

Interactioni

Protein-protein interaction databases

STRINGi9986.ENSOCUP00000013951.

Structurei

Secondary structure

1
134
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi10 – 123Combined sources
Helixi14 – 174Combined sources
Turni18 – 203Combined sources
Beta strandi22 – 254Combined sources
Beta strandi27 – 3610Combined sources
Helixi38 – 403Combined sources
Turni41 – 433Combined sources
Beta strandi44 – 463Combined sources
Helixi49 – 535Combined sources
Turni54 – 563Combined sources
Helixi60 – 667Combined sources
Helixi70 – 7910Combined sources
Beta strandi80 – 845Combined sources
Helixi86 – 883Combined sources
Turni89 – 913Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1DO9NMR-A6-99[»]
2M33NMR-A1-134[»]
ProteinModelPortaliP00169.
SMRiP00169. Positions 6-99.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP00169.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini9 – 8577Cytochrome b5 heme-bindingPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the cytochrome b5 family.Curated
Contains 1 cytochrome b5 heme-binding domain.PROSITE-ProRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG0537. Eukaryota.
COG5274. LUCA.
GeneTreeiENSGT00510000046507.
HOGENOMiHOG000039853.
HOVERGENiHBG002653.
InParanoidiP00169.
OMAiFKSTWII.
OrthoDBiEOG091G17G8.
TreeFamiTF314537.

Family and domain databases

Gene3Di3.10.120.10. 1 hit.
InterProiIPR001199. Cyt_B5-like_heme/steroid-bd.
IPR018506. Cyt_B5_heme-BS.
[Graphical view]
PfamiPF00173. Cyt-b5. 1 hit.
[Graphical view]
PRINTSiPR00363. CYTOCHROMEB5.
SMARTiSM01117. Cyt-b5. 1 hit.
[Graphical view]
SUPFAMiSSF55856. SSF55856. 1 hit.
PROSITEiPS00191. CYTOCHROME_B5_1. 1 hit.
PS50255. CYTOCHROME_B5_2. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: P00169-1) [UniParc]FASTAAdd to basket
Also known as: Liver, Membrane-bound

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAAQSDKDVK YYTLEEIKKH NHSKSTWLIL HHKVYDLTKF LEEHPGGEEV
60 70 80 90 100
LREQAGGDAT ENFEDVGHST DARELSKTFI IGELHPDDRS KLSKPMETLI
110 120 130
TTVDSNSSWW TNWVIPAISA LIVALMYRLY MADD
Length:134
Mass (Da):15,349
Last modified:January 23, 2007 - v4
Checksum:iB748AE9D32FA5E46
GO
Isoform 2 (identifier: P00169-2) [UniParc]FASTAAdd to basket
Also known as: Erythrocyte, Cytoplasmic

The sequence of this isoform differs from the canonical sequence as follows:
     98-98: T → P
     99-134: Missing.

Show »
Length:98
Mass (Da):11,232
Checksum:iC3D197FD9C921C1D
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti62 – 621N → D AA sequence (PubMed:5709273).Curated
Sequence conflicti62 – 621N → D AA sequence (PubMed:5506260).Curated
Sequence conflicti104 – 1041D → N AA sequence (PubMed:500581).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei98 – 981T → P in isoform 2. 1 PublicationVSP_001244
Alternative sequencei99 – 13436Missing in isoform 2. 1 PublicationVSP_001245Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M24844 mRNA. Translation: AAB03878.1.
D10901 mRNA. Translation: BAA01712.1.
PIRiJN0316.
S03373. CBRB5.
RefSeqiNP_001164734.1. NM_001171263.1. [P00169-2]
NP_001164735.1. NM_001171264.1. [P00169-1]
UniGeneiOcu.2112.
Ocu.6903.

Genome annotation databases

EnsembliENSOCUT00000016229; ENSOCUP00000013951; ENSOCUG00000016235. [P00169-1]
GeneIDi100328912.
100328915.
KEGGiocu:100328912.
ocu:100328915.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M24844 mRNA. Translation: AAB03878.1.
D10901 mRNA. Translation: BAA01712.1.
PIRiJN0316.
S03373. CBRB5.
RefSeqiNP_001164734.1. NM_001171263.1. [P00169-2]
NP_001164735.1. NM_001171264.1. [P00169-1]
UniGeneiOcu.2112.
Ocu.6903.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1DO9NMR-A6-99[»]
2M33NMR-A1-134[»]
ProteinModelPortaliP00169.
SMRiP00169. Positions 6-99.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9986.ENSOCUP00000013951.

Proteomic databases

PRIDEiP00169.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSOCUT00000016229; ENSOCUP00000013951; ENSOCUG00000016235. [P00169-1]
GeneIDi100328912.
100328915.
KEGGiocu:100328912.
ocu:100328915.

Phylogenomic databases

eggNOGiKOG0537. Eukaryota.
COG5274. LUCA.
GeneTreeiENSGT00510000046507.
HOGENOMiHOG000039853.
HOVERGENiHBG002653.
InParanoidiP00169.
OMAiFKSTWII.
OrthoDBiEOG091G17G8.
TreeFamiTF314537.

Miscellaneous databases

EvolutionaryTraceiP00169.

Family and domain databases

Gene3Di3.10.120.10. 1 hit.
InterProiIPR001199. Cyt_B5-like_heme/steroid-bd.
IPR018506. Cyt_B5_heme-BS.
[Graphical view]
PfamiPF00173. Cyt-b5. 1 hit.
[Graphical view]
PRINTSiPR00363. CYTOCHROMEB5.
SMARTiSM01117. Cyt-b5. 1 hit.
[Graphical view]
SUPFAMiSSF55856. SSF55856. 1 hit.
PROSITEiPS00191. CYTOCHROME_B5_1. 1 hit.
PS50255. CYTOCHROME_B5_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCYB5_RABIT
AccessioniPrimary (citable) accession number: P00169
Secondary accession number(s): Q28726
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: January 23, 2007
Last modified: September 7, 2016
This is version 134 of the entry and version 4 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.