P00168 (CYB5_ALOSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Cytochrome b5

Gene names

Name:	CYB5A
Synonyms:	CYB5

Organism

Alouatta seniculus (Red howler monkey)

Taxonomic identifier

9503 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Platyrrhini › Atelidae › Alouattinae › Alouatta

Protein attributes

Sequence length	87 AA.
Sequence status	Fragment.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Cytochrome b5 is a membrane bound hemoprotein which function as an electron carrier for several membrane bound oxygenases.
Subcellular location	Endoplasmic reticulum membrane; Single-pass membrane protein; Cytoplasmic side By similarity. Microsome membrane; Single-pass membrane protein; Cytoplasmic side By similarity.
Sequence similarities	Belongs to the cytochrome b5 family. Contains 1 cytochrome b5 heme-binding domain.

Ontologies

Keywords
Biological process	Electron transport Transport
Cellular component	Endoplasmic reticulum Membrane Microsome
Domain	Transmembrane
Ligand	Heme Iron Metal-binding
PTM	Acetylation
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	electron transport chain Inferred from electronic annotation. Source: UniProtKB-KW transport Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	endoplasmic reticulum membrane Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW microsome Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	heme binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – ›87	›87	Cytochrome b5		PRO_0000166007
Regions
	Domain	5 – 81	77	Cytochrome b5 heme-binding
Sites
	Metal binding	40	1	Iron (heme axial ligand)
	Metal binding	64	1	Iron (heme axial ligand)
Amino acid modifications
	Modified residue	15	1	N6-acetyllysine By similarity
Experimental info
	Sequence conflict	11 – 14	4	EEIQ → QEIE AA sequence Ref.1
	Sequence conflict	85 – 87	3	RPK → KPR AA sequence Ref.1
	Non-terminal residue	87	1

Sequences

	Sequence		Length	Mass (Da)
	P00168 [UniParc]. Last modified October 1, 1989. Version 2. Checksum: 5B3552315D1A0F5C	FASTA	87	10,032
	10 20 30 40 50 60 SDEAVKYYTL EEIQKHNHSK STWLILHHKV YDLTKFLEEH PGGEEVLREQ AGGDATEDFE 70 80 DVGHSTDARE LSKTYIIGEL HPDDRPK

References

[1]	"Amino acid sequences of tryptic peptides of cytochromes b5 from microsomes of human, monkey, porcine, and chicken liver." Nobrega F.G., Ozols J. J. Biol. Chem. 246:1706-1717(1971) [PubMed: 4993957] [Abstract] Cited for: PROTEIN SEQUENCE.
[2]	"Structure of cytochrome b5 and its topology in the microsomal membrane." Ozols J. Biochim. Biophys. Acta 997:121-130(1989) [PubMed: 2752049] [Abstract] Cited for: SEQUENCE REVISION TO 11; 14; 85 AND 87.
+	Additional computationally mapped references.

Cross-references

Sequence databases
PIR	S07959.
3D structure databases
ProteinModelPortal	P00168.
SMR	P00168. Positions 1-87.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR001199. Cyt_B5. IPR018506. Cyt_B5_heme-BS. [Graphical view]
Gene3D	G3DSA:3.10.120.10. Cyt_B5. 1 hit.
Pfam	PF00173. Cyt-b5. 1 hit. [Graphical view]
PRINTS	PR00363. CYTOCHROMEB5.
SUPFAM	SSF55856. Cyt_B5. 1 hit.
PROSITE	PS00191. CYTOCHROME_B5_1. 1 hit. PS50255. CYTOCHROME_B5_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CYB5_ALOSE

Accession

Primary (citable) accession number: P00168

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 21, 1986
Last sequence update:	October 1, 1989
Last modified:	August 10, 2010
This is version 78 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program