Reviewed, UniProtKB/Swiss-Prot P00168 (CYB5_ALOSE)

Names and origin

Protein names

Recommended name:
    Cytochrome b5

Gene names

Name:	CYB5A
Synonyms:	CYB5

Organism

Alouatta seniculus (Red howler monkey)

Taxonomic identifier

9503 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Platyrrhini › Atelidae › Alouattinae › Alouatta

Protein attributes

Sequence length	87 AA.
Sequence status	Fragment.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Cytochrome b5 is a membrane bound hemoprotein which function as an electron carrier for several membrane bound oxygenases.
Subcellular location	Endoplasmic reticulum membrane; Single-pass membrane protein; Cytoplasmic side By similarity. Microsome membrane; Single-pass membrane protein; Cytoplasmic side By similarity.
Sequence similarities	Belongs to the cytochrome b5 family. Contains 1 cytochrome b5 heme-binding domain.

Ontologies

Keywords
Biological process	Electron transport Transport
Cellular component	Endoplasmic reticulum Membrane Microsome
Domain	Transmembrane
Ligand	Heme Iron Metal-binding
PTM	Acetylation
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	electron transport chain Inferred from electronic annotation. Source: UniProtKB-KW transport Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	endoplasmic reticulum membrane Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW microsome Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	heme binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – ›87	›87	Cytochrome b5		PRO_0000166007
Regions
	Domain	5 – 81	77	Cytochrome b5 heme-binding
Sites
	Metal binding	40	1	Iron (heme axial ligand)
	Metal binding	64	1	Iron (heme axial ligand)
Amino acid modifications
	Modified residue	15	1	N6-acetyllysine By similarity
Experimental info
	Sequence conflict	11 – 14	4	EEIQ → QEIE AA sequence Ref.1
	Sequence conflict	85 – 87	3	RPK → KPR AA sequence Ref.1
	Non-terminal residue	87	1

Sequences

	Sequence		Length	Mass (Da)
	P00168-1 [UniParc]. Last modified October 1, 1989. Version 2. Checksum: 5B3552315D1A0F5C	FASTA	87	10,032
	10 20 30 40 50 60 SDEAVKYYTL EEIQKHNHSK STWLILHHKV YDLTKFLEEH PGGEEVLREQ AGGDATEDFE 70 80 DVGHSTDARE LSKTYIIGEL HPDDRPK

References

[1]	"Amino acid sequences of tryptic peptides of cytochromes b5 from microsomes of human, monkey, porcine, and chicken liver." Nobrega F.G., Ozols J. J. Biol. Chem. 246:1706-1717(1971) [PubMed: 4993957] [Abstract] Cited for: PROTEIN SEQUENCE.
[2]	"Structure of cytochrome b5 and its topology in the microsomal membrane." Ozols J. Biochim. Biophys. Acta 997:121-130(1989) [PubMed: 2752049] [Abstract] Cited for: SEQUENCE REVISION TO 11; 14; 85 AND 87.

Cross-references

Sequence databases
PIR	S07959.
3D structure databases
HSSP	HSSP built from PDB template 1EHB based on UniProtKB P00171.
SMR	P00168. Positions 3-87.
ModBase	Search...
Phylogenomic databases
HOVERGEN	P00168.
Family and domain databases
InterPro	IPR001199. Cyt_B5. IPR018506. Cyt_B5_heme-BS. [Graphical view]
Gene3D	G3DSA:3.10.120.10. Cyt_B5. 1 hit.
Pfam	PF00173. Cyt-b5. 1 hit. [Graphical view]
PRINTS	PR00363. CYTOCHROMEB5.
ProDom	PD000612. Cyt_B5. 1 hit. [Graphical view] [Entries sharing at least one domain]
PROSITE	PS00191. CYTOCHROME_B5_1. 1 hit. PS50255. CYTOCHROME_B5_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CYB5_ALOSE

Accession

Primary (citable) accession number: P00168

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 21, 1986
Last sequence update:	October 1, 1989
Last modified:	June 16, 2009
This is version 71 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)