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Protein

Cytochrome b

Gene

COB

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Component of the ubiquinol-cytochrome c reductase complex (complex III or cytochrome b-c1 complex), which is part of the mitochondrial respiratory chain that generates an electrochemical potential coupled to ATP synthesis. The complex couples electron transfer from ubiquinol to cytochrome c.

Cofactori

hemeBy similarityNote: Binds 2 heme groups non-covalently.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi82 – 821Iron 1 (heme b562 axial ligand)
Metal bindingi96 – 961Iron 2 (heme b566 axial ligand)
Metal bindingi183 – 1831Iron 1 (heme b562 axial ligand)
Metal bindingi197 – 1971Iron 2 (heme b566 axial ligand)

GO - Molecular functioni

GO - Biological processi

  • aerobic respiration Source: SGD
  • hydrogen ion transmembrane transport Source: GOC
  • mitochondrial electron transport, ubiquinol to cytochrome c Source: SGD
Complete GO annotation...

Keywords - Biological processi

Electron transport, Respiratory chain, Transport

Keywords - Ligandi

Heme, Iron, Metal-binding

Enzyme and pathway databases

BioCyciYEAST:Q0105-MONOMER.

Protein family/group databases

TCDBi3.D.3.3.1. the proton-translocating quinol:cytochrome c reductase (qcr) superfamily.

Names & Taxonomyi

Protein namesi
Recommended name:
Cytochrome b
Alternative name(s):
Complex III subunit 3
Complex III subunit CYTB
Complex III subunit III
Cytochrome b-c1 complex subunit 3
Cytochrome b-c1 complex subunit CYTB
Ubiquinol-cytochrome-c reductase complex cytochrome b subunit
Gene namesi
Name:COB
Synonyms:CYTB
Ordered Locus Names:Q0105
Encoded oniMitochondrion
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Mitochondrion

Organism-specific databases

EuPathDBiFungiDB:Q0105.
SGDiS000007270. COB.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei32 – 5221HelicalSequence analysisAdd
BLAST
Transmembranei85 – 10521HelicalSequence analysisAdd
BLAST
Transmembranei116 – 13621HelicalSequence analysisAdd
BLAST
Transmembranei151 – 17121HelicalSequence analysisAdd
BLAST
Transmembranei179 – 19921HelicalSequence analysisAdd
BLAST
Transmembranei225 – 24521HelicalSequence analysisAdd
BLAST
Transmembranei289 – 30921HelicalSequence analysisAdd
BLAST
Transmembranei324 – 34421HelicalSequence analysisAdd
BLAST
Transmembranei350 – 37021HelicalSequence analysisAdd
BLAST

GO - Cellular componenti

  • mitochondrial respiratory chain complex III Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Membrane, Mitochondrion, Mitochondrion inner membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 385385Cytochrome bPRO_0000061769Add
BLAST

Proteomic databases

TopDownProteomicsiP00163.

Interactioni

Subunit structurei

Fungal cytochrome b-c1 complex contains 10 subunits; 3 respiratory subunits, 2 core proteins and 5 low-molecular weight proteins. Cytochrome b-c1 complex is a homodimer.

Protein-protein interaction databases

BioGridi34779. 30 interactions.
IntActiP00163. 2 interactions.
MINTiMINT-4981526.

Structurei

Secondary structure

1
385
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi3 – 64Combined sources
Helixi10 – 178Combined sources
Beta strandi21 – 233Combined sources
Helixi28 – 314Combined sources
Helixi32 – 5120Combined sources
Turni58 – 603Combined sources
Helixi61 – 7010Combined sources
Helixi75 – 10228Combined sources
Turni103 – 1064Combined sources
Turni108 – 1103Combined sources
Helixi111 – 13525Combined sources
Helixi138 – 14912Combined sources
Helixi150 – 1534Combined sources
Turni155 – 1573Combined sources
Helixi158 – 1669Combined sources
Beta strandi168 – 1714Combined sources
Helixi173 – 20432Combined sources
Beta strandi217 – 2204Combined sources
Turni221 – 2233Combined sources
Helixi224 – 24623Combined sources
Turni248 – 2514Combined sources
Helixi254 – 2574Combined sources
Helixi273 – 2753Combined sources
Helixi276 – 2838Combined sources
Beta strandi285 – 2873Combined sources
Helixi288 – 30013Combined sources
Helixi301 – 3044Combined sources
Helixi305 – 3084Combined sources
Beta strandi312 – 3165Combined sources
Helixi320 – 34021Combined sources
Helixi348 – 36417Combined sources
Helixi366 – 38015Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1EZVX-ray2.30C1-385[»]
1KB9X-ray2.30C1-385[»]
1KYOX-ray2.97C/N1-385[»]
1P84X-ray2.50C1-385[»]
2IBZX-ray2.30C1-385[»]
3CX5X-ray1.90C/N1-385[»]
3CXHX-ray2.50C/N1-385[»]
4PD4X-ray3.04C1-385[»]
ProteinModelPortaliP00163.
SMRiP00163. Positions 1-385.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP00163.

Family & Domainsi

Sequence similaritiesi

Belongs to the cytochrome b family.PROSITE-ProRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

GeneTreeiENSGT00390000017948.
HOGENOMiHOG000255206.
InParanoidiP00163.
KOiK00412.
OMAiPEHIVPE.
OrthoDBiEOG78D7VN.

Family and domain databases

Gene3Di1.20.810.10. 1 hit.
InterProiIPR005798. Cyt_b/b6_C.
IPR005797. Cyt_b/b6_N.
IPR027387. Cytb/b6-like.
IPR030689. Cytochrome_b.
IPR016174. Di-haem_cyt_TM.
[Graphical view]
PfamiPF00032. Cytochrom_B_C. 1 hit.
PF00033. Cytochrome_B. 1 hit.
[Graphical view]
PIRSFiPIRSF038885. COB. 1 hit.
SUPFAMiSSF81342. SSF81342. 1 hit.
SSF81648. SSF81648. 1 hit.
PROSITEiPS51003. CYTB_CTER. 1 hit.
PS51002. CYTB_NTER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P00163-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAFRKSNVYL SLVNSYIIDS PQPSSINYWW NMGSLLGLCL VIQIVTGIFM
60 70 80 90 100
AMHYSSNIEL AFSSVEHIMR DVHNGYILRY LHANGASFFF MVMFMHMAKG
110 120 130 140 150
LYYGSYRSPR VTLWNVGVII FILTIATAFL GYCCVYGQMS HWGATVITNL
160 170 180 190 200
FSAIPFVGND IVSWLWGGFS VSNPTIQRFF ALHYLVPFII AAMVIMHLMA
210 220 230 240 250
LHIHGSSNPL GITGNLDRIP MHSYFIFKDL VTVFLFMLIL ALFVFYSPNT
260 270 280 290 300
LGHPDNYIPG NPLVTPASIV PEWYLLPFYA ILRSIPDKLL GVITMFAAIL
310 320 330 340 350
VLLVLPFTDR SVVRGNTFKV LSKFFFFIFV FNFVLLGQIG ACHVEVPYVL
360 370 380
MGQIATFIYF AYFLIIVPVI STIENVLFYI GRVNK
Length:385
Mass (Da):43,656
Last modified:April 23, 2003 - v3
Checksum:i1B1B1616BBC2A798
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti122 – 1221I → T in CAA58861 (PubMed:7737175).Curated
Sequence conflicti122 – 1221I → T in AAA32151 (PubMed:7004642).Curated
Sequence conflicti269 – 2691I → ID in CAA24073 (PubMed:6253454).Curated
Sequence conflicti269 – 2691I → ID no nucleotide entry (PubMed:6383504).Curated

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti131 – 1311G → S in mutant W7 which is respiratory deficient.

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
V00696 Genomic DNA. Translation: CAA24073.2. Sequence problems.
X84042 mRNA. Translation: CAA58861.1.
KP263414 Genomic DNA. Translation: AIZ98890.1.
J01473, J01472 Genomic DNA. Translation: AAA32151.2.
J01475, J01474 Genomic DNA. Translation: AAA32152.2.
PIRiA00159. CBBY.
S78660.
RefSeqiNP_009315.1. NC_001224.1.

Genome annotation databases

EnsemblFungiiQ0105; Q0105; Q0105.
GeneIDi854583.
KEGGisce:Q0105.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
V00696 Genomic DNA. Translation: CAA24073.2. Sequence problems.
X84042 mRNA. Translation: CAA58861.1.
KP263414 Genomic DNA. Translation: AIZ98890.1.
J01473, J01472 Genomic DNA. Translation: AAA32151.2.
J01475, J01474 Genomic DNA. Translation: AAA32152.2.
PIRiA00159. CBBY.
S78660.
RefSeqiNP_009315.1. NC_001224.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1EZVX-ray2.30C1-385[»]
1KB9X-ray2.30C1-385[»]
1KYOX-ray2.97C/N1-385[»]
1P84X-ray2.50C1-385[»]
2IBZX-ray2.30C1-385[»]
3CX5X-ray1.90C/N1-385[»]
3CXHX-ray2.50C/N1-385[»]
4PD4X-ray3.04C1-385[»]
ProteinModelPortaliP00163.
SMRiP00163. Positions 1-385.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi34779. 30 interactions.
IntActiP00163. 2 interactions.
MINTiMINT-4981526.

Protein family/group databases

TCDBi3.D.3.3.1. the proton-translocating quinol:cytochrome c reductase (qcr) superfamily.

Proteomic databases

TopDownProteomicsiP00163.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiQ0105; Q0105; Q0105.
GeneIDi854583.
KEGGisce:Q0105.

Organism-specific databases

EuPathDBiFungiDB:Q0105.
SGDiS000007270. COB.

Phylogenomic databases

GeneTreeiENSGT00390000017948.
HOGENOMiHOG000255206.
InParanoidiP00163.
KOiK00412.
OMAiPEHIVPE.
OrthoDBiEOG78D7VN.

Enzyme and pathway databases

BioCyciYEAST:Q0105-MONOMER.

Miscellaneous databases

EvolutionaryTraceiP00163.
NextBioi977049.
PROiP00163.

Family and domain databases

Gene3Di1.20.810.10. 1 hit.
InterProiIPR005798. Cyt_b/b6_C.
IPR005797. Cyt_b/b6_N.
IPR027387. Cytb/b6-like.
IPR030689. Cytochrome_b.
IPR016174. Di-haem_cyt_TM.
[Graphical view]
PfamiPF00032. Cytochrom_B_C. 1 hit.
PF00033. Cytochrome_B. 1 hit.
[Graphical view]
PIRSFiPIRSF038885. COB. 1 hit.
SUPFAMiSSF81342. SSF81342. 1 hit.
SSF81648. SSF81648. 1 hit.
PROSITEiPS51003. CYTB_CTER. 1 hit.
PS51002. CYTB_NTER. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Assembly of the mitochondrial membrane system. DNA sequence and organization of the cytochrome b gene in Saccharomyces cerevisiae D273-10B."
    Nobrega F.G., Tzagoloff A.
    J. Biol. Chem. 255:9828-9837(1980) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: D273-10B/A21.
  2. "Revision of the nucleotide sequence at the last intron of the mitochondrial apocytochrome b gene in Saccharomyces cerevisiae."
    Bonjardim C.A., Nobrega F.G.
    Braz. J. Med. Biol. Res. 17:17-20(1984) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: D273-10B/A21.
  3. "Limitations to in vivo import of hydrophobic proteins into yeast mitochondria. The case of a cytoplasmically synthesized apocytochrome b."
    Claros M.G., Perea J., Shu Y., Samatey F.A., Popot J.-L., Jacq C.
    Eur. J. Biochem. 228:762-771(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: WR200.
  4. "The complete sequence of the mitochondrial genome of Saccharomyces cerevisiae."
    Foury F., Roganti T., Lecrenier N., Purnelle B.
    FEBS Lett. 440:325-331(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 96604 / S288c / FY1679.
  5. Cited for: GENOME REANNOTATION.
    Strain: ATCC 96604 / S288c / FY1679.
  6. "Sequence of introns and flanking exons in wild-type and box3 mutants of cytochrome b reveals an interlaced splicing protein coded by an intron."
    Lazowska J., Jacq C., Slonimski P.P.
    Cell 22:333-348(1980) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 20-143.
    Strain: ATCC 44821 / 777-3A.
  7. "Splice points of the third intron in the yeast mitochondrial cytochrome b gene."
    Lazowska J., Jacq C., Slonimski P.P.
    Cell 27:12-14(1981) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 144-169.
    Strain: ATCC 44821 / 777-3A.
  8. "Electron-transfer restoration by vitamin K3 in a complex III-deficient mutant of S. cerevisiae and sequence of the corresponding cytochrome b mutation."
    Brivet-Chevillotte P., di Rago J.-P.
    FEBS Lett. 255:5-9(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: MUTANT W7.
  9. "Structure at 2.3 A resolution of the cytochrome bc1 complex from the yeast Saccharomyces cerevisiae co-crystallized with an antibody Fv fragment."
    Hunte C., Koepke J., Lange C., Rossmanith T., Michel H.
    Structure 8:669-684(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.3 ANGSTROMS).
  10. "Crystal structure of the yeast cytochrome bc1 complex with its bound substrate cytochrome c."
    Lange C., Hunte C.
    Proc. Natl. Acad. Sci. U.S.A. 99:2800-2805(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.97 ANGSTROMS).

Entry informationi

Entry nameiCYB_YEAST
AccessioniPrimary (citable) accession number: P00163
Secondary accession number(s): A0A0A7NYK0
, Q35802, Q35807, Q36301, Q9ZZW9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: April 23, 2003
Last modified: May 11, 2016
This is version 150 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Heme 1 (or BL or b562) is low-potential and absorbs at about 562 nm, and heme 2 (or BH or b566) is high-potential and absorbs at about 566 nm.By similarity

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.